Monitoring of germination ability of conidia of Eutypa lata fungus anamorphic stage isolated from grapevine in Serbia

Abstract

The germination of conidia of anamorphic stage of four Eutypa lata isolates (EL117, EL219, EL227, EL310), previously determined at the molecular level, and two reference isolates BX1.10 and 8F obtained from the Institute National de la Recherche Agronomique, INRA, France was monitored in an in vitro experiment. Conidia germination was performed in two methods. The first method of conidia germination was performed by transferring the conidia to YPDA (Yeast Potato Dexstrose Agar), and the second method of germination of conidia, originating from PDA, MA, GWA and WA media, was performed in a drop of sterile water under UV lights for 24 h. By comparing the germination results using the above methods, it was found that the percentage of conidia germination in both cases was very low. Calculation revealed that the average percentage of conidia germination was very low, 0.12%, when germinated on YPDA medium, while the average percentage of conidia germination by exposure to UV light for 15 days was 0.15%.