Application of multiplex RT-PCR for grapevine viruses detection

Abstract

Grapevine, a significant fruit crop globally, is a host of various viruses that negatively affect yield, plant vigor, and fruit quality. Multiplex polymerase chain reaction (mPCR) offers the ability to detect numerous viruses simultaneously. Our study aimed to evaluate the effectiveness of mRT-PCR for the detection of nine grapevine viruses in Serbia, including: grapevine fanleaf virus, grapevine leafroll-associated viruses 1, 2, and 3, grapevine rupestris stem pitting associated virus, grapevine virus A, grapevine virus B, grapevine fleck virus, and arabis mosaic virus. This study confirms mRT-PCR as an efficient method for simultaneous detection of multiple grapevine viruses