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dc.creatorVasilijević, Bojana
dc.creatorVujović, Tatjana
dc.creatorAnđelić, Tatjana
dc.creatorKatanić, Vera
dc.creatorJevremović, Darko
dc.date.accessioned2024-05-13T10:09:23Z
dc.date.available2024-05-13T10:09:23Z
dc.date.issued2024
dc.identifier.isbn978-86-7078-178-8
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/874
dc.description.abstractThe importance of conserving plant species, particularly those crucial for agriculture, has surged to ensure sustainable utilization of biological resources and prevent further loss of plant diversity. Cryopreservation, the most advanced method for plant conservation, offers promise in this regard. It can be employed for conservation per se, or as a potential tool (cryotherapy) for pathogens elimination from infected plants, particularly viruses that threaten agricultural productivity. In the recent study, two vitrification-based cryopreservation methods, D and V cryo-plate, were evaluated for their efficacy in eradicating plum pox virus (PPV) from autochthonous plum cultivars ‘Crvena Ranka’ and ‘Belošljiva’, widely present in the Balkan region. A total of 111 pool samples of in vitro shoots of plums ‘Belošljiva’ and ‘Crvena Ranka’ were tested (65 and 46 samples, respectively). Each sample consisted of an average of 10 plants, in total about 1100 plants. Health status of in vitro shoots originated from both control (non-frozen explants) and cryopreserved explants of infected plums were continuously tested during multiplication for the PPV presence with conventional reverse transcription polymerase chain reaction (RT-PCR). To evaluate these results and obtain the highest efficiency of detection, all samples were further tested using Real-time PCR (qPCR). Using RT-PCR, the PPV was detected in 67 out of 111 tested samples: in 39 samples of plum ‘Belošljiva’ and 28 of plum ‘Crvena Ranka’. Using a qPCR assay, PPV was confirmed in 82 tested samples. Effective PPV elimination from ‘Crvena Ranka’ was achieved through three cryo-treatments, while neither method (D and V-cryoplate) nor the applied treatments led to PPV elimination in ‘Belošljiva’. The qPCR assay demonstrated higher sensitivity compared to conventional RT-PCR, highlighting its potential utility in such assessments. Our findings supported the exceptional specificity and sensitivity of the qPCR technique in detecting PPV in in vitro shoots of the plums ‘Belošljiva’ and ‘Crvena Ranka’ post-cryotherapy.sr
dc.language.isoensr
dc.publisherBelgrade : Serbian Society for Microbiologysr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200215/RS//sr
dc.rightsopenAccesssr
dc.sourceBook of Abstracts : XIII Congress of microbiologists of Serbia "From biotechnology to human and planetary health", 4th-6th April, 2024, Belgrade, Serbiasr
dc.subjectautochthonous plumssr
dc.subjectplum pox virussr
dc.subjectcryotherapysr
dc.subjectqPCRsr
dc.subjectefficiencysr
dc.titleCryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačaksr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.epage72
dc.citation.spage72
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/2390/bitstream_2390.pdf
dc.type.versionpublishedVersionsr


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