Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots
Само за регистроване кориснике
2024
Чланак у часопису (Објављена верзија)
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The purpose of this work was to apply V and D cryo-plate methods for cryopreservation of Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, and Amelanchier alnifolia (Nutt.) M. Roem. and to monitor the multiplication capacity of shoots regenerated from cryopreserved explants. Shoot tips pre-cultured for 1 d at 23°C in the dark on medium containing 0.3 M sucrose were used as explants. Loading was performed in a solution containing 2 M glycerol and 0.8 M sucrose (30 min at room temperature). In the V cryo-plate, dehydration was carried out at room temperature (20 to 50 min) using the following plant vitrification solutions: original PVS2, 90% PVS2 solution, and PVS3. Regarding the D cryo-plate, dehydration was performed in closed glass containers over silica gel for 2, 2.5, or 3 h. In both protocols, rewarming was carried out in a 1.0 M sucrose solution (15 min at 25°C). Regenerated shoots were multiplied and multiplication parameters were monitored after the second subculture. Us...ing the V cryo-plate method, the highest regrowth in highbush blueberry was obtained following 50-min treatment with all three VSs (61.7 to 80.9%). The D cryo-plate method was even more suitable with maximum regrowth of 89.4% achieved after 2.5 h of desiccation. For strawberry, 62.5% was the highest regrowth recorded using PVS3-based V cryo-plate method while 83.3% of regrowth was observed using D cryo-plate protocol. Regrowth of saskatoon reached a maximum of 50% after 50-min treatment with PVS3 while it did not exceed 40% in other treatments. By the second subculture, shoots regenerated from cryopreserved explants regained and even exceeded the multiplication capacity of shoots regenerated from non-cryopreserved explants. This study is the first to present the successful application of the V cryo-plate method in highbush blueberry, as well as the utilization of both V and D cryo-plate methods in saskatoon.
Кључне речи:
Highbush blueberry / strawberry / saskatoon / vitrification solution / desiccationИзвор:
In Vitro Cellular & Developmental Biology - Plant, 2024Издавач:
- Secaucus, N.J. : Springer Science + Business Media
Финансирање / пројекти:
- Operational program Integrated Infrastructure within the project: Demand-driven research for the sustainable and innovative food, Drive4SIFood 313011V336, cofinanced by the European Regional Development Fund
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200215 (Институт за воћарство, Чачак) (RS-MESTD-inst-2020-200215)
Институција/група
Institut za voćarstvoTY - JOUR AU - Vujović, Tatjana AU - Anđelić, Tatjana AU - Marković, Zvjezdana AU - Gajdošova, Alena AU - Hunkova, Julia PY - 2024 UR - https://refri.institut-cacak.org/handle/123456789/858 AB - The purpose of this work was to apply V and D cryo-plate methods for cryopreservation of Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, and Amelanchier alnifolia (Nutt.) M. Roem. and to monitor the multiplication capacity of shoots regenerated from cryopreserved explants. Shoot tips pre-cultured for 1 d at 23°C in the dark on medium containing 0.3 M sucrose were used as explants. Loading was performed in a solution containing 2 M glycerol and 0.8 M sucrose (30 min at room temperature). In the V cryo-plate, dehydration was carried out at room temperature (20 to 50 min) using the following plant vitrification solutions: original PVS2, 90% PVS2 solution, and PVS3. Regarding the D cryo-plate, dehydration was performed in closed glass containers over silica gel for 2, 2.5, or 3 h. In both protocols, rewarming was carried out in a 1.0 M sucrose solution (15 min at 25°C). Regenerated shoots were multiplied and multiplication parameters were monitored after the second subculture. Using the V cryo-plate method, the highest regrowth in highbush blueberry was obtained following 50-min treatment with all three VSs (61.7 to 80.9%). The D cryo-plate method was even more suitable with maximum regrowth of 89.4% achieved after 2.5 h of desiccation. For strawberry, 62.5% was the highest regrowth recorded using PVS3-based V cryo-plate method while 83.3% of regrowth was observed using D cryo-plate protocol. Regrowth of saskatoon reached a maximum of 50% after 50-min treatment with PVS3 while it did not exceed 40% in other treatments. By the second subculture, shoots regenerated from cryopreserved explants regained and even exceeded the multiplication capacity of shoots regenerated from non-cryopreserved explants. This study is the first to present the successful application of the V cryo-plate method in highbush blueberry, as well as the utilization of both V and D cryo-plate methods in saskatoon. PB - Secaucus, N.J. : Springer Science + Business Media T2 - In Vitro Cellular & Developmental Biology - Plant T1 - Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots DO - 10.1007/s11627-023-10399-5 ER -
@article{ author = "Vujović, Tatjana and Anđelić, Tatjana and Marković, Zvjezdana and Gajdošova, Alena and Hunkova, Julia", year = "2024", abstract = "The purpose of this work was to apply V and D cryo-plate methods for cryopreservation of Vaccinium corymbosum ʻToroʼ, Fragaria × ananassa ʻCleryʼ, and Amelanchier alnifolia (Nutt.) M. Roem. and to monitor the multiplication capacity of shoots regenerated from cryopreserved explants. Shoot tips pre-cultured for 1 d at 23°C in the dark on medium containing 0.3 M sucrose were used as explants. Loading was performed in a solution containing 2 M glycerol and 0.8 M sucrose (30 min at room temperature). In the V cryo-plate, dehydration was carried out at room temperature (20 to 50 min) using the following plant vitrification solutions: original PVS2, 90% PVS2 solution, and PVS3. Regarding the D cryo-plate, dehydration was performed in closed glass containers over silica gel for 2, 2.5, or 3 h. In both protocols, rewarming was carried out in a 1.0 M sucrose solution (15 min at 25°C). Regenerated shoots were multiplied and multiplication parameters were monitored after the second subculture. Using the V cryo-plate method, the highest regrowth in highbush blueberry was obtained following 50-min treatment with all three VSs (61.7 to 80.9%). The D cryo-plate method was even more suitable with maximum regrowth of 89.4% achieved after 2.5 h of desiccation. For strawberry, 62.5% was the highest regrowth recorded using PVS3-based V cryo-plate method while 83.3% of regrowth was observed using D cryo-plate protocol. Regrowth of saskatoon reached a maximum of 50% after 50-min treatment with PVS3 while it did not exceed 40% in other treatments. By the second subculture, shoots regenerated from cryopreserved explants regained and even exceeded the multiplication capacity of shoots regenerated from non-cryopreserved explants. This study is the first to present the successful application of the V cryo-plate method in highbush blueberry, as well as the utilization of both V and D cryo-plate methods in saskatoon.", publisher = "Secaucus, N.J. : Springer Science + Business Media", journal = "In Vitro Cellular & Developmental Biology - Plant", title = "Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots", doi = "10.1007/s11627-023-10399-5" }
Vujović, T., Anđelić, T., Marković, Z., Gajdošova, A.,& Hunkova, J.. (2024). Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots. in In Vitro Cellular & Developmental Biology - Plant Secaucus, N.J. : Springer Science + Business Media.. https://doi.org/10.1007/s11627-023-10399-5
Vujović T, Anđelić T, Marković Z, Gajdošova A, Hunkova J. Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots. in In Vitro Cellular & Developmental Biology - Plant. 2024;. doi:10.1007/s11627-023-10399-5 .
Vujović, Tatjana, Anđelić, Tatjana, Marković, Zvjezdana, Gajdošova, Alena, Hunkova, Julia, "Cryopreservation of highbush blueberry, strawberry, and saskatoon using V and D cryo‑plate methods and monitoring of multiplication ability of regenerated shoots" in In Vitro Cellular & Developmental Biology - Plant (2024), https://doi.org/10.1007/s11627-023-10399-5 . .