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The allelic polymorphism of ETR1 gene in Malus Miller species

dc.creatorMarić, Slađana
dc.creatorRadičević, Sanja
dc.creatorMilošević, Nebojša
dc.creatorLukić, Milan
dc.creatorGlišić, Ivana
dc.creatorĐorđević, Milena
dc.date.accessioned2023-12-04T14:09:27Z
dc.date.available2023-12-04T14:09:27Z
dc.date.issued2022
dc.identifier.isbn978-86-7520-548-7
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/820
dc.description.abstractDozrevanje klimakteričnog ploda jabuke praćeno je naglim povećanjem brzine respiracije i produkcije etilena. U proces etilenske percepcije i transdukcije signala uključena je familija receptora lokalizovana na membrane endoplazmatičnog retikuluma. Etilen receptor 1 (ETR1) je jedan od etilenskih receptora, kodiran ETR1 genom. U radu su predstavljeni rezultati proučavanja alelnog polimorfizma ETR1 gena kod devet ex situ kolekcionisanih vrsta roda Malus: Malus × atrosanguinea (Spaeth) Schneid., Malus floribunda Siebold ex Van Houtte, Malus hupehensis (Pamp.) Rehder, Malus × micromalus Makino, Malus niedzwetzkyana Dieck, Malus prunifolia (Willd.) Borkh. var. microcarpa, Malus prunifolia (Willd.) Borkh. var. xanthocarpa, Malus sargentii Rehder i Malus sieboldii (Regel) Rehder. Determinacija ETR1-genotipa ispitivanih vrsta jabuke sprovedena je restrikcionom analizom PCR-om umnoženog genomskog fragmenta (~5.000 bp) sa RsaI, AluI i HinfI enzimima. Nakon digestije PCR proizvoda ETR1 gena sa RsaI enzimom, kod svih proučavanih vrsta jabuke uočeni su polimorfni regioni sa fragmentima od 800 bp (f1), 890 bp (f2), 1.050 bp (f3), 1.130 bp (f4) i 1.300 bp (f5). Restrikcijom sa AluI enzimom dobijen je polimorfni fragment veličine 850 bp (f1), dok je digestijom sa HinfI enzimom detektovan fragment veličine 1.130 bp (f2). U ispitivanom materijalu identifikovano je šest alela – a, b, c, d, f i g, od kojih su dva (aleli f i g) po prvi put otkrivena u okviru ovih istraživanja; utvrđeno je šest alelnih konstitucija ‒ bb, bd, bf, b,a/c, ff i g,a/c/d/g. Dalja karakterizacija novoidentifikovanih f i g alela biće sprovedena njihovim kloniranjem i sekvenciranjem. Od devet analiziranih vrsta, po jedna ima alelnu konstituciju bb, bd i bf, dok je kod dve vrste utvrđen fenotip ff; kod tri vrste identifikovan je alel b, dok bi drugi na osnovu fenotipa mogao biti alel a ili alel c; kod jedne vrste identifikovan je alel g, dok bi drugi mogao biti jedan od alela a, c, d ili g.sr
dc.description.abstractThe ripening of climacteric apple fruit is associated with an upsurge in the rate of respiration and ethylene production. The family of endoplasmic reticulum-localized receptors is involved in ethylene perception and signal transduction. Ethylene Receptor 1 (ETR1) is one of ethylene receptors, encoded by ETR1 gene. This work was aimed to study the allelic polymorphism of ETR1 gene in nine ex situMalus species: Malus × atrosanguinea (Spaeth) Schneid., Malus floribunda Siebold ex Van Houtte, Malus hupehensis (Pamp.) Rehder, Malus × micromalus Makino, Malus niedzwetzkyana Dieck, Malus prunifolia (Willd.) Borkh. var. microcarpa, Malus prunifolia (Willd.) Borkh. var. xanthocarpa, Malus sargentii Rehder and Malus sieboldii (Regel) Rehder. Determination of ETR1-genotype was based on restriction analysis of PCR amplified genomic fragment (~5,000 bp) with RsaI, AluI and HinfI. Upon digestion of the ETR1 product with RsaI, the polymorphic regions with fragments of 800 bp (f1), 890 bp (f2), 1,050 bp (f3), 1,130 bp (f4) and 1,300 bp (f5) were observed. Restriction with AluI and HinfIrevealed the segregating fragments of 850 bp (f1) and 1,130 bp (f2), respectively. The comparison of the polymorphisms resulted in the identification of six ETR1 alleles – a, b, c, d, f and g, among which two (f and g) were revealed in this study for the first time; six allelic constitutions were generated ‒ bb, bd, bf, b,a/c, ff and g,a/c/d/g. Cloning and sequencing of the DNA fragments corresponding to novel alleles will provide their further characterisation. Of the evaluated species, one species each was scored as bb, bd and bf, while the phenotype ff was identified in two species; the allele b was identified in three species and the second allele might be a or c; the allele g was identified in one species and according to the phenotype the second allele might be a, c, d or g.sr
dc.language.isosrsr
dc.language.isoensr
dc.publisherNovi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultetsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200215/RS//sr
dc.rightsopenAccesssr
dc.sourceZbornik apstrakata : 16. kongres voćara i vinogradara Srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbijasr
dc.subjectjabukasr
dc.subjectetilen receptorsr
dc.subjectalelsr
dc.subjecttrajašnost plodasr
dc.subjectapplesr
dc.subjectethylene receptorsr
dc.subjectallelesr
dc.subjectfruit keeping qualitysr
dc.titleAlelni polimorfizam ETR1 gena kod vrsta roda Malus Millersr
dc.titleThe allelic polymorphism of ETR1 gene in Malus Miller speciessr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.epage83
dc.citation.spage82
dc.description.otherČlanovi Programskog odbora 16. kongresa voćara i vinogradara Srbije: Aleksandar Leposavić, Žaklina Karaklajić-Stajić, Sanja Radičević, Tatjana Vujović, Darko Jevremović, Branko Popović, Nebojša Milošević i Slađana Marić. Članovi Organizacionog odbora 16. kongresa voćara i vinogradara Srbije: Marijana Pešaković i Ivana Glišić.sr
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/2050/bitstream_2050.pdf
dc.type.versionpublishedVersionsr


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