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dc.creatorJevremović, Darko
dc.creatorZejak, Dejan
dc.creatorVasilijević, Bojana
dc.creatorLeposavić, Aleksandar
dc.date.accessioned2023-11-22T09:05:35Z
dc.date.available2023-11-22T09:05:35Z
dc.date.issued2023
dc.identifier.isbn978-3-031-47466-8
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/787
dc.description.abstractHighbush blueberries (Vaccinium corymbosum L.) are a recent addition to Montenegro’s fruit producing repertoire. The northern region of the country is home to new, dense plantations. To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars were collected and analyzed. The samples were tested by ELISA for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). Assays were conducted according to the recommended protocols. The samples were further tested for the presence of blueberry mosaic-associated virus and blueberry latent virus using RT-PCR and for the presence of the blueberry red ringspot virus using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was done with pd(N)6 random primers, and PCR was done with virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide. The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars ‘’Duke’, ‘Bluecrop’, and ‘Earlyblue’. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples. This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.sr
dc.language.isoensr
dc.publisherSpringersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200215/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceLecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challengessr
dc.subjectVaccinium corymbosum L.sr
dc.subjectVirusessr
dc.subjectELISAsr
dc.subjectPCRsr
dc.subjectLatent infectionsr
dc.titleDetection of blueberry latent virus on highbush blueberries in Montenegrosr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.epage29
dc.citation.spage23
dc.type.versionpublishedVersionsr


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