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dc.creatorĐorđević, Milena
dc.creatorVujović, Tatjana
dc.creatorCerović, Radosav
dc.creatorGlišić, Ivana
dc.creatorMilošević, Nebojša
dc.creatorMarić, Slađana
dc.creatorRadičević, Sanja
dc.date.accessioned2023-11-09T12:28:41Z
dc.date.available2023-11-09T12:28:41Z
dc.date.issued2021
dc.identifier.isbn978-86-920869-2-2
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/761
dc.description.abstractBreeding programmes often require the necessity for longer pollen storage, whether asynchronous flowering of parental pairs exists (especially if early-flowering genotypes are pollinated with late-flowering), or if there is a temporal, geographical or spatial distance. The objective of this study was to assessed pollen vitality in vitro and in vivo in three plum cultivars (‘Čačanka Lepotica’, ‘Valjevka’ and ‘Valerija’) after one year of storage at +4°C and sub-freezing temperatures (-20°C, -80°C and -196°C). Pollen viability in vitro was determined on germination medium and by staining with fluorescein-diacetate. For in vivo vitality assay, stored pollen was used for pollination of emasculated flowers of ‘Čačanka Lepotica’. Parameters of pollen tubes growth was determined on 3th, 6th and 10th day after pollination. In all cultivars, the lowest rate of pollen viability was observed for that stored at +4°C. Pollen stored at sub-freezing temperatures showed relatively high viability that was reduced from 8.85 to 47.15% in comparison with fresh pollen. Generaly, in vitro germination test showed lower pollen viability compared to fluorescein-diacetate staining method. Pollen viability under in vivo conditions, corellated with the those obtained under in vitro conditions. The growth of pollen tubes, from pollen stored at +4°C, or was absent or it ended mainly in the upper parts of the style. For pollen stored at sub-freezing temperatures, pollen tubes penetrated into the nucelus on the 6th day after pollination in all cultivars. The best dynamic of pollen tube growth of ‘Čačanska Lepotica’ and ‘Valerija’ was observed with pollen stored at -80°C, while in ‘Valјevka’ with that stored at -196°C.sr
dc.language.isoensr
dc.publisherČačak : Fruit Research Institutesr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200215/RS//sr
dc.rightsopenAccesssr
dc.sourceProgramme and Book of Abstract : XII International Symposium of Plum and Prune Genetics, Breeding and Pomology, 14 - 17 September, 2021sr
dc.subjectPrunus domestica L.sr
dc.subjectpollen storagesr
dc.subjectpollen germination in vitrosr
dc.subjectpollen germination in vivosr
dc.titleReproductive ability of plum (Prunus domestica L.) pollen stored at low temperaturessr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.epage56
dc.citation.spage56
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/1798/bitstream_1798.pdf
dc.type.versionpublishedVersionsr


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