Raspberry leaf blotch emaravirus (RLBV) is recently characterized virus infecting Rubus species. Recent studies in Serbia confirmed its wide presence in red raspberry orchards. The objective of our study was to investigate RLBV presence in the plants and its possible effects on pollen performance in vitro in two cultivars (‘Meeker’ and ‘Willamette’) in the region of west Serbia. In both cultivars, pollen vitality and pollen germination were compared between healthy and infected plants. From all tested plants, flowers were picked randomly in late balloon phase. Samples were analyzed on RLBV presence by RT-PCR. Pollen vitality was determined after staining with fluorescin-diacetate while pollen germination was observed after 1h, 3h and 24h incubation time on agar-sucrose medium. In both cultivars, in healthy plants, a high percentage of pollen vitality was got (75,94% in ‘Meeker’, 64,97% in ‘Willamette’). Also, in these plants with longer incubation time pollen germination in vitro incre...ased significantly. In infected plants, a different picture was obtained. With a longer incubation period, pollen grain germination in ‘Willamette’ had slower growth, while the ‘Meeker’ varieties were in decline.
TY - CONF
AU - Đorđevic, Milena
AU - Cerović, Radosav
AU - Leposavić, Aleksandar
AU - Paunović, Svetlana A.
AU - Vujović, Tatjana
AU - Jevremović, Darko
PY - 2019
UR - https://refri.institut-cacak.org/handle/123456789/758
AB - Raspberry leaf blotch emaravirus (RLBV) is recently characterized virus infecting Rubus species. Recent studies in Serbia confirmed its wide presence in red raspberry orchards. The objective of our study was to investigate RLBV presence in the plants and its possible effects on pollen performance in vitro in two cultivars (‘Meeker’ and ‘Willamette’) in the region of west Serbia. In both cultivars, pollen vitality and pollen germination were compared between healthy and infected plants. From all tested plants, flowers were picked randomly in late balloon phase. Samples were analyzed on RLBV presence by RT-PCR. Pollen vitality was determined after staining with fluorescin-diacetate while pollen germination was observed after 1h, 3h and 24h incubation time on agar-sucrose medium. In both cultivars, in healthy plants, a high percentage of pollen vitality was got (75,94% in ‘Meeker’, 64,97% in ‘Willamette’). Also, in these plants with longer incubation time pollen germination in vitro increased significantly. In infected plants, a different picture was obtained. With a longer incubation period, pollen grain germination in ‘Willamette’ had slower growth, while the ‘Meeker’ varieties were in decline.
C3 - The Book of Abstracts and Symposium Programme : 4th Balkan Symposium on Fruit Growing, 14 - 18 September, Istanbul, Turkey
T1 - Effect of Raspberry leaf blotch emaravirus on raspberry (Rubus idaeus L.) pollen performance in vitro
EP - 51
SP - 51
ER -
@conference{
author = "Đorđevic, Milena and Cerović, Radosav and Leposavić, Aleksandar and Paunović, Svetlana A. and Vujović, Tatjana and Jevremović, Darko",
year = "2019",
abstract = "Raspberry leaf blotch emaravirus (RLBV) is recently characterized virus infecting Rubus species. Recent studies in Serbia confirmed its wide presence in red raspberry orchards. The objective of our study was to investigate RLBV presence in the plants and its possible effects on pollen performance in vitro in two cultivars (‘Meeker’ and ‘Willamette’) in the region of west Serbia. In both cultivars, pollen vitality and pollen germination were compared between healthy and infected plants. From all tested plants, flowers were picked randomly in late balloon phase. Samples were analyzed on RLBV presence by RT-PCR. Pollen vitality was determined after staining with fluorescin-diacetate while pollen germination was observed after 1h, 3h and 24h incubation time on agar-sucrose medium. In both cultivars, in healthy plants, a high percentage of pollen vitality was got (75,94% in ‘Meeker’, 64,97% in ‘Willamette’). Also, in these plants with longer incubation time pollen germination in vitro increased significantly. In infected plants, a different picture was obtained. With a longer incubation period, pollen grain germination in ‘Willamette’ had slower growth, while the ‘Meeker’ varieties were in decline.",
journal = "The Book of Abstracts and Symposium Programme : 4th Balkan Symposium on Fruit Growing, 14 - 18 September, Istanbul, Turkey",
title = "Effect of Raspberry leaf blotch emaravirus on raspberry (Rubus idaeus L.) pollen performance in vitro",
pages = "51-51"
}
Đorđevic, M., Cerović, R., Leposavić, A., Paunović, S. A., Vujović, T.,& Jevremović, D.. (2019). Effect of Raspberry leaf blotch emaravirus on raspberry (Rubus idaeus L.) pollen performance in vitro. in The Book of Abstracts and Symposium Programme : 4th Balkan Symposium on Fruit Growing, 14 - 18 September, Istanbul, Turkey, 51-51.
Đorđevic M, Cerović R, Leposavić A, Paunović SA, Vujović T, Jevremović D. Effect of Raspberry leaf blotch emaravirus on raspberry (Rubus idaeus L.) pollen performance in vitro. in The Book of Abstracts and Symposium Programme : 4th Balkan Symposium on Fruit Growing, 14 - 18 September, Istanbul, Turkey. 2019;:51-51..
Đorđevic, Milena, Cerović, Radosav, Leposavić, Aleksandar, Paunović, Svetlana A., Vujović, Tatjana, Jevremović, Darko, "Effect of Raspberry leaf blotch emaravirus on raspberry (Rubus idaeus L.) pollen performance in vitro" in The Book of Abstracts and Symposium Programme : 4th Balkan Symposium on Fruit Growing, 14 - 18 September, Istanbul, Turkey (2019):51-51.
