Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants
Authors
Nikolić, BiljanaVasilijević, Bojana

Ćirić, Ana
Mitić-Ćulafić, Dragana
Cvetković, Stefana
Džamić, Ana
Knežević-Vukčević, Jelena
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Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesi...on of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis.
Keywords:
Juniperus communis / essential oil / post-distillation waste / selective antimicrobial effect / adhesion-inhibition propertiesSource:
Archives of Biological Sciences, 2019, 71, 2, 235-244Publisher:
- Belgrade : Serbian Biological Society
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-173032)
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Institut za voćarstvoTY - JOUR AU - Nikolić, Biljana AU - Vasilijević, Bojana AU - Ćirić, Ana AU - Mitić-Ćulafić, Dragana AU - Cvetković, Stefana AU - Džamić, Ana AU - Knežević-Vukčević, Jelena PY - 2019 UR - https://refri.institut-cacak.org/handle/123456789/635 AB - Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis. PB - Belgrade : Serbian Biological Society T2 - Archives of Biological Sciences T1 - Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants EP - 244 IS - 2 SP - 235 VL - 71 DO - 10.2298/ABS181217005N ER -
@article{ author = "Nikolić, Biljana and Vasilijević, Bojana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Cvetković, Stefana and Džamić, Ana and Knežević-Vukčević, Jelena", year = "2019", abstract = "Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis.", publisher = "Belgrade : Serbian Biological Society", journal = "Archives of Biological Sciences", title = "Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants", pages = "244-235", number = "2", volume = "71", doi = "10.2298/ABS181217005N" }
Nikolić, B., Vasilijević, B., Ćirić, A., Mitić-Ćulafić, D., Cvetković, S., Džamić, A.,& Knežević-Vukčević, J.. (2019). Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants. in Archives of Biological Sciences Belgrade : Serbian Biological Society., 71(2), 235-244. https://doi.org/10.2298/ABS181217005N
Nikolić B, Vasilijević B, Ćirić A, Mitić-Ćulafić D, Cvetković S, Džamić A, Knežević-Vukčević J. Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants. in Archives of Biological Sciences. 2019;71(2):235-244. doi:10.2298/ABS181217005N .
Nikolić, Biljana, Vasilijević, Bojana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Cvetković, Stefana, Džamić, Ana, Knežević-Vukčević, Jelena, "Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants" in Archives of Biological Sciences, 71, no. 2 (2019):235-244, https://doi.org/10.2298/ABS181217005N . .