Show simple item record

dc.creatorNikolić, Biljana
dc.creatorVasilijević, Bojana
dc.creatorKnežević-Vukčević, Jelena
dc.creatorOrčić, Dejan
dc.creatorĆirić, Ana
dc.creatorDzamić, Ana
dc.creatorAnačkov, Goran
dc.creatorRajčević, Nemanja
dc.creatorMitić-Ćulafić, Dragana
dc.date.accessioned2023-10-10T12:33:28Z
dc.date.available2023-10-10T12:33:28Z
dc.date.issued2019
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/634
dc.description.abstractPlant material, collected in July 2014 near the peak Babin Zub, was used to prepare essential oil (EO) and post-distillation waste (PDW). GC-MS determined α- pinen as dominant EO constituent, while LC-MS/MS pointed at rutin and quinic acid as the most abundant in PDW. Strong antioxidativity of PDW was determined in DPPH (IC50 5.27µg/mL) assay. Cytotoxicity against carcinoma cells of lung (A549) and colon (HT-29 and HCT116), and normal fibroblasts (MRC-5), pointed at remarkably higher effectivity of EO (IC50 range 69-160µg/mL). Binary mixtures of EO/PDW with doxorubicin induced clear synergism, being more pronounced in cancer A549 cells than in normal fibroblasts. Microdilution assay performed on selected fungal and bacterial strains showed that PDW induced moderate antimicrobial effect against fungi (MICs and MFCs ranging between 118-900µg/mL) and Listeria monocytogenes (MIC/MBC were 390/740 µg/mL). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in checkerboard assay. High selectivity of PDW against fungi and L. monocytogenes was determined in respect to all used cells. In in vitro adhesion-inhibition assay PDW reduced adhesion of L. monocytogenes to colon cells (29-62% inhibition). Obtained results indicated that J. communis EO could be recommended for further anticancer study, while PDW should be considered as potential antimicrobial.sr
dc.language.isoensr
dc.publisherNiš : University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecologysr
dc.publisherBelgrade: Institute for Nature Conservation of Serbiasr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172058/RS//sr
dc.rightsopenAccesssr
dc.sourceAbstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbiasr
dc.titleNew insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effectsr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.epage141
dc.citation.spage141
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/1189/bitstream_1189.pdf
dc.type.versionpublishedVersionsr


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record