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New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect
Plant material, collected in July 2014 near the peak Babin Zub, was used to
prepare essential oil (EO) and post-distillation waste (PDW). GC-MS determined α-
pinen as dominant EO constituent, while LC-MS/MS pointed at rutin and quinic acid
as the most abundant in PDW. Strong antioxidativity of PDW was determined in
DPPH (IC50 5.27µg/mL) assay. Cytotoxicity against carcinoma cells of lung (A549)
and colon (HT-29 and HCT116), and normal fibroblasts (MRC-5), pointed at
remarkably higher effectivity of EO (IC50 range 69-160µg/mL). Binary mixtures of
EO/PDW with doxorubicin induced clear synergism, being more pronounced in
cancer A549 cells than in normal fibroblasts. Microdilution assay performed on
selected fungal and bacterial strains showed that PDW induced moderate
antimicrobial effect against fungi (MICs and MFCs ranging between 118-900µg/mL)
and Listeria monocytogenes (MIC/MBC were 390/740 µg/mL). Combinations of
EO/PDW with selected antibiotics induced synergistic antili...sterial activity in
checkerboard assay. High selectivity of PDW against fungi and L. monocytogenes was
determined in respect to all used cells. In in vitro adhesion-inhibition assay PDW
reduced adhesion of L. monocytogenes to colon cells (29-62% inhibition). Obtained
results indicated that J. communis EO could be recommended for further anticancer
study, while PDW should be considered as potential antimicrobial.
Source:
Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia, 2019, 141-141
Publisher:
Niš : University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology
Belgrade: Institute for Nature Conservation of Serbia
TY - CONF
AU - Nikolić, Biljana
AU - Vasilijević, Bojana
AU - Knežević-Vukčević, Jelena
AU - Orčić, Dejan
AU - Ćirić, Ana
AU - Dzamić, Ana
AU - Anačkov, Goran
AU - Rajčević, Nemanja
AU - Mitić-Ćulafić, Dragana
PY - 2019
UR - https://refri.institut-cacak.org/handle/123456789/634
AB - Plant material, collected in July 2014 near the peak Babin Zub, was used to
prepare essential oil (EO) and post-distillation waste (PDW). GC-MS determined α-
pinen as dominant EO constituent, while LC-MS/MS pointed at rutin and quinic acid
as the most abundant in PDW. Strong antioxidativity of PDW was determined in
DPPH (IC50 5.27µg/mL) assay. Cytotoxicity against carcinoma cells of lung (A549)
and colon (HT-29 and HCT116), and normal fibroblasts (MRC-5), pointed at
remarkably higher effectivity of EO (IC50 range 69-160µg/mL). Binary mixtures of
EO/PDW with doxorubicin induced clear synergism, being more pronounced in
cancer A549 cells than in normal fibroblasts. Microdilution assay performed on
selected fungal and bacterial strains showed that PDW induced moderate
antimicrobial effect against fungi (MICs and MFCs ranging between 118-900µg/mL)
and Listeria monocytogenes (MIC/MBC were 390/740 µg/mL). Combinations of
EO/PDW with selected antibiotics induced synergistic antilisterial activity in
checkerboard assay. High selectivity of PDW against fungi and L. monocytogenes was
determined in respect to all used cells. In in vitro adhesion-inhibition assay PDW
reduced adhesion of L. monocytogenes to colon cells (29-62% inhibition). Obtained
results indicated that J. communis EO could be recommended for further anticancer
study, while PDW should be considered as potential antimicrobial.
PB - Niš : University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology
PB - Belgrade: Institute for Nature Conservation of Serbia
C3 - Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia
T1 - New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect
EP - 141
SP - 141
ER -
@conference{
author = "Nikolić, Biljana and Vasilijević, Bojana and Knežević-Vukčević, Jelena and Orčić, Dejan and Ćirić, Ana and Dzamić, Ana and Anačkov, Goran and Rajčević, Nemanja and Mitić-Ćulafić, Dragana",
year = "2019",
abstract = "Plant material, collected in July 2014 near the peak Babin Zub, was used toprepare essential oil (EO) and post-distillation waste (PDW). GC-MS determined α-pinen as dominant EO constituent, while LC-MS/MS pointed at rutin and quinic acidas the most abundant in PDW. Strong antioxidativity of PDW was determined inDPPH (IC50 5.27µg/mL) assay. Cytotoxicity against carcinoma cells of lung (A549)and colon (HT-29 and HCT116), and normal fibroblasts (MRC-5), pointed atremarkably higher effectivity of EO (IC50 range 69-160µg/mL). Binary mixtures ofEO/PDW with doxorubicin induced clear synergism, being more pronounced incancer A549 cells than in normal fibroblasts. Microdilution assay performed onselected fungal and bacterial strains showed that PDW induced moderateantimicrobial effect against fungi (MICs and MFCs ranging between 118-900µg/mL)and Listeria monocytogenes (MIC/MBC were 390/740 µg/mL). Combinations ofEO/PDW with selected antibiotics induced synergistic antilisterial activity incheckerboard assay. High selectivity of PDW against fungi and L. monocytogenes wasdetermined in respect to all used cells. In in vitro adhesion-inhibition assay PDWreduced adhesion of L. monocytogenes to colon cells (29-62% inhibition). Obtainedresults indicated that J. communis EO could be recommended for further anticancerstudy, while PDW should be considered as potential antimicrobial.",
publisher = "Niš : University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Belgrade: Institute for Nature Conservation of Serbia",
journal = "Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia",
title = "New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect",
pages = "141-141"
}
Nikolić, B., Vasilijević, B., Knežević-Vukčević, J., Orčić, D., Ćirić, A., Dzamić, A., Anačkov, G., Rajčević, N.,& Mitić-Ćulafić, D.. (2019). New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect. in Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia
Niš : University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology., 141-141.
Nikolić B, Vasilijević B, Knežević-Vukčević J, Orčić D, Ćirić A, Dzamić A, Anačkov G, Rajčević N, Mitić-Ćulafić D. New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect. in Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia. 2019;:141-141..
Nikolić, Biljana, Vasilijević, Bojana, Knežević-Vukčević, Jelena, Orčić, Dejan, Ćirić, Ana, Dzamić, Ana, Anačkov, Goran, Rajčević, Nemanja, Mitić-Ćulafić, Dragana, "New insights into biological potential of Juniperus communis L. var. saxatilis Pall. from Stara Planina Mt: in vitro cytotoxic and antimicrobial effect" in Abstracts : 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20-23.6. 2019, Stara planina, Serbia (2019):141-141.
