Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona
Juniperus communis essential oil and post-destilation waste: antibacterial effect against food contaminants and in vitro cytotoxicity against human colon cells
Аутори
Cvetković, StefanaVasilijević, Bojana
![](/themes/MirageReFRI/images/orcid.png)
Mitić-Ćulafić, Dragana
Knežević-Vukčević, Jelena
Orčić, Dejan
![](/themes/MirageReFRI/images/orcid.png)
Nikolić, Biljana
Конференцијски прилог (Објављена верзија)
![](/themes/MirageReFRI//images/creativecommons/arr.png)
Метаподаци
Приказ свих података о документуАпстракт
Listeria monocytogenes je patogen hrane, izrazito tolerantan prema različitim faktorima spoljašnje sredine, uključujući
temperaturu, što doprinosi čestoj pojavi u namirnicama. Cilj ovog rada bio je ispitivanje antibakterijskog potencijala etarskih
ulja Juniperus communis (JC-EO) i Satureja montana (SM-EO), kao i njihova hemijska karakterizacija. Antibakterijski
efekat je ispitivan prema referentnom ATCC 19111 soju, kao i prema primoizolatima sa junećeg trupa (LMB), lososa (LMS)
i odvodnog tunela vode u klanici (LMT). Primenjeni su mikrodilucioni testovi (MIC i metoda šahovske table) i metoda
vremenski zavisne inhibicije rasta, koja se izvodi u makro-volumenima.
GC-MS analiza je ukazala da su dominantni sastojci JC-EO α-pinen (47,8%) i sabinen (11,0%), a SM-EO karvakrol (30.7%)
i timol (18.0%). Minimalne inhibitorne koncentracije (MIC) oba ulja iznosile su 0,5-1%, dok su minimalne baktericidne
koncentracije bile 1-4% za JC-EO i 1% za SM-EO. Metoda šahovske table, primenjena za a...nalizu kombinovanog dejstva
oba ulja, ukazala je na sve tipove interakcije u slučaju primoizolata i pretežno indiferentni efekat prema ATCC 19111 soju.
Metoda vremenski zavisne inhibicije rasta potvrdila je dobijene tipove interakcije. Pored toga, analizom kriva inhibicije
rasta i izračunavanjem stopa rasta za svaku od njih, utvrđene su koncentracije ulja koje su dovele do odsustva i rasta i
inhibicije (MIC vrednosti kriva, cMIC). One su bile značajno niže od vrednosti MIC dobijenih u mikrodilucionoj metodi,
što se pripisuje različitim uslovima aerobioze ostvarenim u mikro i makro volumenima, odnosno metaboličkim razlikama
koje ih prate.
U zaključku možemo konstatovati da etarska ulja J. communis i S. montana mogu efikasno inhibirati rast L. monocytogenes.
Dalja evaluacija njihovog antibakterijskog potencijala in vitro i in situ u hrani može se preporučiti za buduća istraživanja.
Listeria monocytogenes is a food pathogen widely tolerant to different environmental factors including temperature. This is
the main factor contributing to frequent food contamination. The aim of this study was to examine the antibacterial effect of
essential oils of Juniperus communis (JC-EO) and Satureja montana (SM-EO) against L. monocytogenes, as well as to
determine their chemical composition. Antibacterial effect was determined against reference ATCC 19111 and three L.
monocytogenes primoisolates: LMB, LMS and LMT, isolated from beef carcass, salmon and slaughterhouse water drainage
tunnel, respectively. It was estimated in microdilution MIC and checkerboard assays, and in time kill assay, performed in
macro-volumes.
The chemical analysis, determined by GC-MS, revealed that the most abundant constituents were α-pinene (47.8%) and
sabinene (11.0%) in JC-EO, and carvacrol (30.7%) and thymol (18.0%) in SM-EO. Minimal inhibitory concentrations
(MICs) of both EOs were in the ...range 0.5-1%. Minimal bactericidal concentrations were determined to be 1-4% for JC-EO,
and 1% for SM-EO. Checkerboard assay, performed in order to analyze the combined effect of the oils, revealed all types
of interactions for tested primoisolates, and mostly indifferent effect against ATCC 19111 strain. Time kill assay confirmed
the obtained mode of interactions. In addition, calculation of growth rates was used to estimate the concentrations inducing
neither growth, nor killing effect (curve MIC values, cMICs). They were multifold lower compared to MICs obtained in
microdilution assay. This could be adressed to different aerobiosis realized in micro- and macro-volumes, and consequent
metabolic differences.
In conclusion, J. communis and S. montana essential oils and their combinations could be the efficient vehicles inhibiting
the growth of L. monocytogenes. Further evaluation of their antibacterial potential, both in vitro and in situ in foods, could
be suggested.
Извор:
Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd, 2018, HZP1 / FHP1-Издавач:
- Beograd : Univerzitet u Beogradu
Институција/група
Institut za voćarstvoTY - CONF AU - Cvetković, Stefana AU - Vasilijević, Bojana AU - Mitić-Ćulafić, Dragana AU - Knežević-Vukčević, Jelena AU - Orčić, Dejan AU - Nikolić, Biljana PY - 2018 UR - https://refri.institut-cacak.org/handle/123456789/630 AB - Listeria monocytogenes je patogen hrane, izrazito tolerantan prema različitim faktorima spoljašnje sredine, uključujući temperaturu, što doprinosi čestoj pojavi u namirnicama. Cilj ovog rada bio je ispitivanje antibakterijskog potencijala etarskih ulja Juniperus communis (JC-EO) i Satureja montana (SM-EO), kao i njihova hemijska karakterizacija. Antibakterijski efekat je ispitivan prema referentnom ATCC 19111 soju, kao i prema primoizolatima sa junećeg trupa (LMB), lososa (LMS) i odvodnog tunela vode u klanici (LMT). Primenjeni su mikrodilucioni testovi (MIC i metoda šahovske table) i metoda vremenski zavisne inhibicije rasta, koja se izvodi u makro-volumenima. GC-MS analiza je ukazala da su dominantni sastojci JC-EO α-pinen (47,8%) i sabinen (11,0%), a SM-EO karvakrol (30.7%) i timol (18.0%). Minimalne inhibitorne koncentracije (MIC) oba ulja iznosile su 0,5-1%, dok su minimalne baktericidne koncentracije bile 1-4% za JC-EO i 1% za SM-EO. Metoda šahovske table, primenjena za analizu kombinovanog dejstva oba ulja, ukazala je na sve tipove interakcije u slučaju primoizolata i pretežno indiferentni efekat prema ATCC 19111 soju. Metoda vremenski zavisne inhibicije rasta potvrdila je dobijene tipove interakcije. Pored toga, analizom kriva inhibicije rasta i izračunavanjem stopa rasta za svaku od njih, utvrđene su koncentracije ulja koje su dovele do odsustva i rasta i inhibicije (MIC vrednosti kriva, cMIC). One su bile značajno niže od vrednosti MIC dobijenih u mikrodilucionoj metodi, što se pripisuje različitim uslovima aerobioze ostvarenim u mikro i makro volumenima, odnosno metaboličkim razlikama koje ih prate. U zaključku možemo konstatovati da etarska ulja J. communis i S. montana mogu efikasno inhibirati rast L. monocytogenes. Dalja evaluacija njihovog antibakterijskog potencijala in vitro i in situ u hrani može se preporučiti za buduća istraživanja. AB - Listeria monocytogenes is a food pathogen widely tolerant to different environmental factors including temperature. This is the main factor contributing to frequent food contamination. The aim of this study was to examine the antibacterial effect of essential oils of Juniperus communis (JC-EO) and Satureja montana (SM-EO) against L. monocytogenes, as well as to determine their chemical composition. Antibacterial effect was determined against reference ATCC 19111 and three L. monocytogenes primoisolates: LMB, LMS and LMT, isolated from beef carcass, salmon and slaughterhouse water drainage tunnel, respectively. It was estimated in microdilution MIC and checkerboard assays, and in time kill assay, performed in macro-volumes. The chemical analysis, determined by GC-MS, revealed that the most abundant constituents were α-pinene (47.8%) and sabinene (11.0%) in JC-EO, and carvacrol (30.7%) and thymol (18.0%) in SM-EO. Minimal inhibitory concentrations (MICs) of both EOs were in the range 0.5-1%. Minimal bactericidal concentrations were determined to be 1-4% for JC-EO, and 1% for SM-EO. Checkerboard assay, performed in order to analyze the combined effect of the oils, revealed all types of interactions for tested primoisolates, and mostly indifferent effect against ATCC 19111 strain. Time kill assay confirmed the obtained mode of interactions. In addition, calculation of growth rates was used to estimate the concentrations inducing neither growth, nor killing effect (curve MIC values, cMICs). They were multifold lower compared to MICs obtained in microdilution assay. This could be adressed to different aerobiosis realized in micro- and macro-volumes, and consequent metabolic differences. In conclusion, J. communis and S. montana essential oils and their combinations could be the efficient vehicles inhibiting the growth of L. monocytogenes. Further evaluation of their antibacterial potential, both in vitro and in situ in foods, could be suggested. PB - Beograd : Univerzitet u Beogradu C3 - Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd T1 - Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona T1 - Juniperus communis essential oil and post-destilation waste: antibacterial effect against food contaminants and in vitro cytotoxicity against human colon cells SP - HZP1 / FHP1 ER -
@conference{ author = "Cvetković, Stefana and Vasilijević, Bojana and Mitić-Ćulafić, Dragana and Knežević-Vukčević, Jelena and Orčić, Dejan and Nikolić, Biljana", year = "2018", abstract = "Listeria monocytogenes je patogen hrane, izrazito tolerantan prema različitim faktorima spoljašnje sredine, uključujućitemperaturu, što doprinosi čestoj pojavi u namirnicama. Cilj ovog rada bio je ispitivanje antibakterijskog potencijala etarskihulja Juniperus communis (JC-EO) i Satureja montana (SM-EO), kao i njihova hemijska karakterizacija. Antibakterijskiefekat je ispitivan prema referentnom ATCC 19111 soju, kao i prema primoizolatima sa junećeg trupa (LMB), lososa (LMS)i odvodnog tunela vode u klanici (LMT). Primenjeni su mikrodilucioni testovi (MIC i metoda šahovske table) i metodavremenski zavisne inhibicije rasta, koja se izvodi u makro-volumenima.GC-MS analiza je ukazala da su dominantni sastojci JC-EO α-pinen (47,8%) i sabinen (11,0%), a SM-EO karvakrol (30.7%)i timol (18.0%). Minimalne inhibitorne koncentracije (MIC) oba ulja iznosile su 0,5-1%, dok su minimalne baktericidnekoncentracije bile 1-4% za JC-EO i 1% za SM-EO. Metoda šahovske table, primenjena za analizu kombinovanog dejstvaoba ulja, ukazala je na sve tipove interakcije u slučaju primoizolata i pretežno indiferentni efekat prema ATCC 19111 soju.Metoda vremenski zavisne inhibicije rasta potvrdila je dobijene tipove interakcije. Pored toga, analizom kriva inhibicijerasta i izračunavanjem stopa rasta za svaku od njih, utvrđene su koncentracije ulja koje su dovele do odsustva i rasta iinhibicije (MIC vrednosti kriva, cMIC). One su bile značajno niže od vrednosti MIC dobijenih u mikrodilucionoj metodi,što se pripisuje različitim uslovima aerobioze ostvarenim u mikro i makro volumenima, odnosno metaboličkim razlikamakoje ih prate.U zaključku možemo konstatovati da etarska ulja J. communis i S. montana mogu efikasno inhibirati rast L. monocytogenes.Dalja evaluacija njihovog antibakterijskog potencijala in vitro i in situ u hrani može se preporučiti za buduća istraživanja., Listeria monocytogenes is a food pathogen widely tolerant to different environmental factors including temperature. This isthe main factor contributing to frequent food contamination. The aim of this study was to examine the antibacterial effect ofessential oils of Juniperus communis (JC-EO) and Satureja montana (SM-EO) against L. monocytogenes, as well as todetermine their chemical composition. Antibacterial effect was determined against reference ATCC 19111 and three L.monocytogenes primoisolates: LMB, LMS and LMT, isolated from beef carcass, salmon and slaughterhouse water drainagetunnel, respectively. It was estimated in microdilution MIC and checkerboard assays, and in time kill assay, performed inmacro-volumes.The chemical analysis, determined by GC-MS, revealed that the most abundant constituents were α-pinene (47.8%) andsabinene (11.0%) in JC-EO, and carvacrol (30.7%) and thymol (18.0%) in SM-EO. Minimal inhibitory concentrations(MICs) of both EOs were in the range 0.5-1%. Minimal bactericidal concentrations were determined to be 1-4% for JC-EO,and 1% for SM-EO. Checkerboard assay, performed in order to analyze the combined effect of the oils, revealed all typesof interactions for tested primoisolates, and mostly indifferent effect against ATCC 19111 strain. Time kill assay confirmedthe obtained mode of interactions. In addition, calculation of growth rates was used to estimate the concentrations inducingneither growth, nor killing effect (curve MIC values, cMICs). They were multifold lower compared to MICs obtained inmicrodilution assay. This could be adressed to different aerobiosis realized in micro- and macro-volumes, and consequentmetabolic differences.In conclusion, J. communis and S. montana essential oils and their combinations could be the efficient vehicles inhibitingthe growth of L. monocytogenes. Further evaluation of their antibacterial potential, both in vitro and in situ in foods, couldbe suggested.", publisher = "Beograd : Univerzitet u Beogradu", journal = "Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd", title = "Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona, Juniperus communis essential oil and post-destilation waste: antibacterial effect against food contaminants and in vitro cytotoxicity against human colon cells", pages = "HZP1 / FHP1" }
Cvetković, S., Vasilijević, B., Mitić-Ćulafić, D., Knežević-Vukčević, J., Orčić, D.,& Nikolić, B.. (2018). Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona. in Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd Beograd : Univerzitet u Beogradu., HZP1 / FHP1.
Cvetković S, Vasilijević B, Mitić-Ćulafić D, Knežević-Vukčević J, Orčić D, Nikolić B. Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona. in Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd. 2018;:HZP1 / FHP1..
Cvetković, Stefana, Vasilijević, Bojana, Mitić-Ćulafić, Dragana, Knežević-Vukčević, Jelena, Orčić, Dejan, Nikolić, Biljana, "Etarsko ulje i postdestilacioni ostatak Juniperus communis: antibakterijski efekat prema kontaminentima hrane i in vitro citotoksični potencijal na humanim ćelijama kolona" in Program; i zbornik radova : UNIFOOD CONFERENCE, 5 i 6 oktobar 2018, Beograd (2018):HZP1 / FHP1.