Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations
Authors
Nikolić, BiljanaManojlović, Dragica
Dramićanin, Miroslav
Miletić, Vesna
Knežević-Vukčević, Jelena
Vasilijević, Bojana
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Mitić-Ćulafić, Dragana
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Resin-based composites are used in dentistry as restorative materials or adhesives. Their
unbound components may diffuse through dentin into the pulp or be swallowed with saliva;
in both cases they could be systemically distributed by blood circulation. To prevent possible
adverse effects of such uptake, the study of biocompatibility is extremely important. In
this work we studied cytotoxicity and genotoxicity of dental materials on human fetal lung
fibroblasts MRC-5, by MTT and comet assay. Tested materials were novel urethane -based
monomer FIT and the conventional one BisGMA, co-monomer TEGDMA, and photoinitiator
systems - the new Lucirin TPO (TPO), and conventional photoiniator camphor quinone (CQ)
and its co-iniator DMAEMA. They were tested individually and in mixtures of composites
that are actually being used in dental practice: FIT+TEGDMA and CQ+DMAEMA (C-
1), FIT+TEGDMA and TPO (C-2), BisGMA+TEGDMA and CQ+DMAEMA (C-3), and
BisGMA+TEGDMA and TPO (C-4).
Results obtai...ned revealed that cytotoxicity and genotoxicity increased in the following
orders: TEGDMA<DMAEMA<CQ<FIT<TPO<BisGMA, and TEGDMA< CQ<FIT<BisGMA<TPO,
respectively. Moreover, DMAEMA was not genotoxic (up to 75 µg mL-1). Concerning the
effects of the composite mixtures, the manner in which cytotoxicity increased was C-2<C-
1<C-3<C-4, while the pattern in which genotoxicity increased was C-2<C-3<C-1<C-4. The
results obtained favored the novel monomer FIT, due to lower cytotoxicity and genotoxicity
than the conventional monomer BisGMA. On the contrary, new photoiniator TPO could not
be recommended, due to the multifold higher genotoxicity than both CQ and DMAEMA.
Results support the importance of genotoxicity testing, due to different ranges of genotoxic
and cytotoxic activity. Furthermore, the study of complex composites seems to be of special
interest, due to the fact that both antagonism and synergism were observed for certain
mixtures.
Keywords:
Biocompatibility / resin-based composites / MTT assay / comet assaySource:
Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia, 2019, 300, 117-117Publisher:
- Belgrade : Serbian Genetic Society
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Institut za voćarstvoTY - CONF AU - Nikolić, Biljana AU - Manojlović, Dragica AU - Dramićanin, Miroslav AU - Miletić, Vesna AU - Knežević-Vukčević, Jelena AU - Vasilijević, Bojana AU - Mitić-Ćulafić, Dragana PY - 2019 UR - https://refri.institut-cacak.org/handle/123456789/621 AB - Resin-based composites are used in dentistry as restorative materials or adhesives. Their unbound components may diffuse through dentin into the pulp or be swallowed with saliva; in both cases they could be systemically distributed by blood circulation. To prevent possible adverse effects of such uptake, the study of biocompatibility is extremely important. In this work we studied cytotoxicity and genotoxicity of dental materials on human fetal lung fibroblasts MRC-5, by MTT and comet assay. Tested materials were novel urethane -based monomer FIT and the conventional one BisGMA, co-monomer TEGDMA, and photoinitiator systems - the new Lucirin TPO (TPO), and conventional photoiniator camphor quinone (CQ) and its co-iniator DMAEMA. They were tested individually and in mixtures of composites that are actually being used in dental practice: FIT+TEGDMA and CQ+DMAEMA (C- 1), FIT+TEGDMA and TPO (C-2), BisGMA+TEGDMA and CQ+DMAEMA (C-3), and BisGMA+TEGDMA and TPO (C-4). Results obtained revealed that cytotoxicity and genotoxicity increased in the following orders: TEGDMA<DMAEMA<CQ<FIT<TPO<BisGMA, and TEGDMA< CQ<FIT<BisGMA<TPO, respectively. Moreover, DMAEMA was not genotoxic (up to 75 µg mL-1). Concerning the effects of the composite mixtures, the manner in which cytotoxicity increased was C-2<C- 1<C-3<C-4, while the pattern in which genotoxicity increased was C-2<C-3<C-1<C-4. The results obtained favored the novel monomer FIT, due to lower cytotoxicity and genotoxicity than the conventional monomer BisGMA. On the contrary, new photoiniator TPO could not be recommended, due to the multifold higher genotoxicity than both CQ and DMAEMA. Results support the importance of genotoxicity testing, due to different ranges of genotoxic and cytotoxic activity. Furthermore, the study of complex composites seems to be of special interest, due to the fact that both antagonism and synergism were observed for certain mixtures. PB - Belgrade : Serbian Genetic Society C3 - Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia T1 - Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations EP - 117 SP - 117 VL - 300 ER -
@conference{ author = "Nikolić, Biljana and Manojlović, Dragica and Dramićanin, Miroslav and Miletić, Vesna and Knežević-Vukčević, Jelena and Vasilijević, Bojana and Mitić-Ćulafić, Dragana", year = "2019", abstract = "Resin-based composites are used in dentistry as restorative materials or adhesives. Theirunbound components may diffuse through dentin into the pulp or be swallowed with saliva;in both cases they could be systemically distributed by blood circulation. To prevent possibleadverse effects of such uptake, the study of biocompatibility is extremely important. Inthis work we studied cytotoxicity and genotoxicity of dental materials on human fetal lungfibroblasts MRC-5, by MTT and comet assay. Tested materials were novel urethane -basedmonomer FIT and the conventional one BisGMA, co-monomer TEGDMA, and photoinitiatorsystems - the new Lucirin TPO (TPO), and conventional photoiniator camphor quinone (CQ)and its co-iniator DMAEMA. They were tested individually and in mixtures of compositesthat are actually being used in dental practice: FIT+TEGDMA and CQ+DMAEMA (C-1), FIT+TEGDMA and TPO (C-2), BisGMA+TEGDMA and CQ+DMAEMA (C-3), andBisGMA+TEGDMA and TPO (C-4).Results obtained revealed that cytotoxicity and genotoxicity increased in the followingorders: TEGDMA<DMAEMA<CQ<FIT<TPO<BisGMA, and TEGDMA< CQ<FIT<BisGMA<TPO,respectively. Moreover, DMAEMA was not genotoxic (up to 75 µg mL-1). Concerning theeffects of the composite mixtures, the manner in which cytotoxicity increased was C-2<C-1<C-3<C-4, while the pattern in which genotoxicity increased was C-2<C-3<C-1<C-4. Theresults obtained favored the novel monomer FIT, due to lower cytotoxicity and genotoxicitythan the conventional monomer BisGMA. On the contrary, new photoiniator TPO could notbe recommended, due to the multifold higher genotoxicity than both CQ and DMAEMA.Results support the importance of genotoxicity testing, due to different ranges of genotoxicand cytotoxic activity. Furthermore, the study of complex composites seems to be of specialinterest, due to the fact that both antagonism and synergism were observed for certainmixtures.", publisher = "Belgrade : Serbian Genetic Society", journal = "Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia", title = "Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations", pages = "117-117", volume = "300" }
Nikolić, B., Manojlović, D., Dramićanin, M., Miletić, V., Knežević-Vukčević, J., Vasilijević, B.,& Mitić-Ćulafić, D.. (2019). Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations. in Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia Belgrade : Serbian Genetic Society., 300, 117-117.
Nikolić B, Manojlović D, Dramićanin M, Miletić V, Knežević-Vukčević J, Vasilijević B, Mitić-Ćulafić D. Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations. in Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia. 2019;300:117-117..
Nikolić, Biljana, Manojlović, Dragica, Dramićanin, Miroslav, Miletić, Vesna, Knežević-Vukčević, Jelena, Vasilijević, Bojana, Mitić-Ćulafić, Dragana, "Biocompatibility of resin-based composites: comparative analysis of cytotoxicity and genotoxicity of constituents and their combinations" in Book of abstract: 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, Serbia, 300 (2019):117-117.