In vitro antilisterial effect of essential oils by modeling of growth curve MIC values
Authors
Nikolić, BiljanaVasilijević, Bojana

Jovanović, Marina
Knežević-Vukčević, Jelena
Djekic, Ilija
Cvetković, Stefana
Djukanović, Stefana
Mitić-Ćulafić, Dragana
Conference object (Published version)

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Background: Listeria monocytogenes is food contaminant being widely tolerant to different
environmental factors. It is one of the most frequent causative agents of deaths among foodborne
pathogens.
Objectives: The aim was to examine the in vitro antilisterial effect of essential oils (EOs) of Juniperus
communis and Satureja montana.
Methods: EOs were chemically analyzed by GC/GC-MS. Antilisterial effect against ATCC19111 strain
and three primoisolates was determined in microdilution, checkerboard and time-kill assays. Results
of time-kill assay were used not only to analyze the type of interaction between EOs, but also to
determine the growth rates of curves obtained for different concentrations of test substances. By
plotting of growth rates as a function of applied concentrations, growth curve MIC was determined
as the concentration inducing neither the progression nor the regression of bacterial population.
Results: MIC values determined in microdilution assay and by mode...ling of time-kill assay growth
curves were in the ranges 0.5-1% and 0.03-0.14%, respectively. Multifold higher values of MIC
obtained in microdilution assay could be addressed to the feature that L. monocytogenes is more
resistant to different stressors in reduced oxygen environment. In addition, synergism of both EOs in
mixture treatment of isolates was determined in checkerboard and time-kill assays. In conclusion, J.
communis and S. montana EOs and their combinations could be the efficient inhibitors of the L.
monocytogenes growth. However, the fact that its susceptibility to antimicrobials is influenced with
environmental conditions requires the use of different experimental approaches in order to better
explore it.
Source:
Abstract Book : 8th Congress of European Microbiologists, 7-11 July, 2019, Glasgow, Scotland, 2019, 1553-1553Publisher:
- Delft : Federation of European Microbiological Societies