Detection of blueberry latent virus on highbush blueberries in Montenegro
Apstrakt
The highbush blueberry (Vaccinium corymbosum L.) is a relatively new fruit species for fruit growing in Montenegro. Established plantations are located in the northern part of the country.
To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars (‘Aurora’, ‘Bluecrop’, ‘Brigita Blue’, ‘Duke’, and ‘Earlyblue’) were collected and analyzed.
The samples were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). The samples were further tested for the presence of blueberry mosaic-associated virus (BlMaV) and blueberry latent virus (BlLV) using RT-PCR and for the presence of the blueberry red ringspot virus (BRRV) using PCR. Extraction of total nucle...ic acids was performed with 2% CTAB buffer. Reverse transcription was performed using pd(N)6 random primers, and PCR reactions were performed using virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide.
The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars 'Duke', 'Bluecrop', and 'Earlyblue'. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples.
This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.
Ključne reči:
Vaccinium corymbosum L. / viruses / ELISA / PCR / latencyIzvor:
Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina, 2022, 68-68Izdavač:
- Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences
Institucija/grupa
Institut za voćarstvoTY - CONF AU - Jevremović, Darko AU - Zejak, Dejan AU - Vasilijević, Bojana AU - Leposavić, Aleksandar PY - 2022 UR - https://refri.institut-cacak.org/handle/123456789/564 AB - The highbush blueberry (Vaccinium corymbosum L.) is a relatively new fruit species for fruit growing in Montenegro. Established plantations are located in the northern part of the country. To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars (‘Aurora’, ‘Bluecrop’, ‘Brigita Blue’, ‘Duke’, and ‘Earlyblue’) were collected and analyzed. The samples were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). The samples were further tested for the presence of blueberry mosaic-associated virus (BlMaV) and blueberry latent virus (BlLV) using RT-PCR and for the presence of the blueberry red ringspot virus (BRRV) using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was performed using pd(N)6 random primers, and PCR reactions were performed using virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide. The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars 'Duke', 'Bluecrop', and 'Earlyblue'. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples. This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country. PB - Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences C3 - Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina T1 - Detection of blueberry latent virus on highbush blueberries in Montenegro EP - 68 SP - 68 ER -
@conference{ author = "Jevremović, Darko and Zejak, Dejan and Vasilijević, Bojana and Leposavić, Aleksandar", year = "2022", abstract = "The highbush blueberry (Vaccinium corymbosum L.) is a relatively new fruit species for fruit growing in Montenegro. Established plantations are located in the northern part of the country.To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars (‘Aurora’, ‘Bluecrop’, ‘Brigita Blue’, ‘Duke’, and ‘Earlyblue’) were collected and analyzed.The samples were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). The samples were further tested for the presence of blueberry mosaic-associated virus (BlMaV) and blueberry latent virus (BlLV) using RT-PCR and for the presence of the blueberry red ringspot virus (BRRV) using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was performed using pd(N)6 random primers, and PCR reactions were performed using virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide.The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars 'Duke', 'Bluecrop', and 'Earlyblue'. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples.This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.", publisher = "Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences", journal = "Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina", title = "Detection of blueberry latent virus on highbush blueberries in Montenegro", pages = "68-68" }
Jevremović, D., Zejak, D., Vasilijević, B.,& Leposavić, A.. (2022). Detection of blueberry latent virus on highbush blueberries in Montenegro. in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences., 68-68.
Jevremović D, Zejak D, Vasilijević B, Leposavić A. Detection of blueberry latent virus on highbush blueberries in Montenegro. in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina. 2022;:68-68..
Jevremović, Darko, Zejak, Dejan, Vasilijević, Bojana, Leposavić, Aleksandar, "Detection of blueberry latent virus on highbush blueberries in Montenegro" in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina (2022):68-68.