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Production of highbush blueberry nuclear stock in Serbia

Производство на нуклеарен материал от високохрастовидна американска боровинка в Сърбия

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2018
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Authors
Jevremović, Darko
Leposavić, Aleksandar
Paunović, Svetlana A.
Article (Published version)
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Abstract
The highbush blueberry (Vaccinium corymbosum L.) is becoming increasingly important small fruit crop in Serbian agriculture. According to some estimations, highbush blueberries are cultivated on approximately 700 ha in Serbia. In 2015, a program for the production of certified highbush blueberry pathogen-tested material has started in the Fruit Research Institute in Čačak. A total of 52 candidate clones of 10 cultivars were selected during 2015 and 2016. Plants were planted in a sterile medium and put in the insect-proof screen-house. Since 2016, plants were regularly visually checked for the presence of bacteria, fungi and other diseases or unknown symptoms. All plants were annually tested by ELISA for the presence of Blueberry shock virus, Blueberry mottle virus, Blueberry scorch virus, Blueberry shoestring virus, Tomato ringspot virus, Tobacco ringspot virus and Peach rosette mosaic virus; and by PCR on Blueberry red ringspot virus, Blueberry mosaic-associated virus and phytoplasmas.... Samples from candidate clones were also indexed on the herbaceous indicator Cucumis sativus. None of these pathogens have been detected in tested candidate clones so far.

Високохрастовидната американска боровинка (Vaccinium corymbosum L.) става все поважна ягодоплодна култура в сръбското селско стопанство. Според някои оценки, храстовидната боровинка се отглеждат на около 700 ha в Сърбия. През 2015 г. в Изследователски институт по овощарство в Чачак започна програма за производство на сертифициран материал, pathogen-tested. През 2015 и 2016 г. са селектирани общо 52 кандидат-клона от 10 сорта. Растенията са засадени в стерилна среда, поставени под мрежи за защита от насекоми. От 2016 г. Растенията редовно се проверяват визуално за наличие на бактериални, гъбни и други болести или неизвестни симптоми. Всички растения ежегодно са изследвани, чрез ELISA, за наличието на Blueberry shock virus, Blueberry mottle virus, Blueberry scorch virus, Blueberry shoestring virus, Tomato ringspot virus, Tobacco ringspot virus и Peach rosette mosaic virus и чрез PCR за Blueberry red ringspot virus, мозаечен вирус и фитоплазми. Проби от кандидатa-клоновете са индексирани ...също и върху тревистия индикатор Cucumis sativus. Нито един от тези патогени не е открит досега в изследваните кандидат-клонове.

Keywords:
highbush blueberry / planting material / certification / viruses / phytoplasmas / високохрастовидна американска боровинка / посадъчен материал / сертифициране / вируси / фитоплазма
Source:
Journal of Mountain Agriculture on the Balkans, 2018, 21, 5, 207-213
Publisher:
  • Troyan : Research Institute of Mountain Stockbreeding and Agriculture
Funding / projects:
  • Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia

ISSN: 1311-0489

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URI
https://refri.institut-cacak.org/handle/123456789/523
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  • Radovi istraživača / Researchers’ publications
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Institut za voćarstvo

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