Detection of peach latent mosaic viroid by RT-PCR and real-time PCR
Abstract
Peach latent mosaic viroid (PLMVd) is economically the most important viroid
infecting stone fruits. In sensitive peach cultivars PLMVd induces mosaic and
calico on the leaves and deformed fruits with cracked suture. Early and accurate
detection of PLMVd is one of the main measures for its control. In our study we
have evaluated the efficiency of RT-PCR and quantitative Real-time PCR methods
for the detection of PLMVd isolates collected from peach and nectarine orchards
in Serbia. RT-PCR assay proved to be efficient for PLMVd detection, but some of
the isolates were on the limit of detection. Real-time RCR was characterized by
higher sensitivity in the detection of all tested isolates. Both assays can be
recommended for use, but for testing the material within the certification of peach
planting material Real-time PCR should be used.
Keywords:
peach / PLMVd / reverse transcription / PCR / Real-time PCRSource:
Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia, 2022, 309-316Publisher:
- Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences