Study of Eutypa lata isolates originating from Serbia

Abstract

The potential influence of different types of light on the growth and sporulation of Eutypa lata isolates was assessed, and detection at the molecular level was also performed. By applying in vitro observations, the influence of different types of light was monitored, (24 h exposure to UV light; alternating 12 h UV and 12 h darkness; alternating 12 h artificial fluorescent light and 12 h darkness), on the radial growth and sporulation of the anamorphic stage of three isolates of Eutypa lata fungus (EL117, EL153 and EL199) by comparison with two reference isolates of Eutypa lata, BX1.10 and 8F, obtained from the INRA, France. After the molecular detection using of the specific primer pair Lata 1/ Lata 2.2, all the studied isolates were found to belong to the species Eutypa lata (syn. Eutypa armeniacae), anamorph Libertella blepharis. It was also found that the most suitable type of light for radial growth and sporulation of the studied isolates is exposure to 24 h UV light for 30 days.

U ovom radu je proučavan jedan od uzročnika odumiranja čokota vinove loze gljiva Eutypa lata. Praćen je uticaj različitih tipova svetlosti na porast i sporulaciju izolata Eutipa lata, a izvršena je i detekcija na molekularnom nivou. Naime, u in vitro uslovima praćen je uticaj različitih tipova svetlosti (24 h izlaganje uticaju UV svetla (UV); alternativno, 12 h UV i 12 h tama (UV-T); alternativno, 12 h veštačko fluorescentno svetlo i 12 h tama (ST)) na radijalni porast i sporulaciju anamorfnog stadijuma tri izolata gljive Eutypa lata (EL117, EL153 and EL199) upoređivanjem sa dva referentna izolata Eutypa lata BX1.10 i 8F dobijenih sa Institute National de la Recherche Agronomique, INRA, France. Takođe je urađena detekcija izolata na molekularnom nivou primenom specifičnog para prajmera Lata 1/Lata 2.2. Nakon sprovedene molekularne detekcije, utvrđeno je da svi proučavani izolati pripadaju vrsti Eutypa lata (syn. Eutypa armeniacae), anamorf Libertella blepharis. Takođe je ustanovljeno da je najpogodniji tip svetlosti za radijalni porast i sporulaciju, proučavanih izolata izlaganje, 24 h UV svetlu u trajanju od 30 dana.