Application of qPCR for plum pox virus detection during cryotherapy
Abstract
For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryotreated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR assay showed higher sensitivity in comparison to conventional RT-PCR.
Keywords:
plum pox virus / qPCR / cryotherapy / efficiencySource:
Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023, 2023, 283-288Publisher:
- Kragujevac : University, Faculty of Agronomy in Čačak
Funding / projects:
- CryoPlum - Conservation and Plum Pox Virus Eradication From Serbian Autochthonous Plum Genotypes Using Cryotechniques (RS-6062279)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200215 (Fruit Research Institute, Čačak) (RS-200215)