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Application of qPCR for plum pox virus detection during cryotherapy

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2023
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Authors
Jevremović, Darko
Vasilijević, Bojana
Anđelić, Tatjana
Vujović, Tatjana
Conference object (Published version)
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Abstract
For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryotreated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR assay showed higher sensitivity in comparison to conventional RT-PCR.
Keywords:
plum pox virus / qPCR / cryotherapy / efficiency
Source:
Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023, 2023, 283-288
Publisher:
  • Kragujevac : University, Faculty of Agronomy in Čačak
Funding / projects:
  • CryoPlum - Conservation and Plum Pox Virus Eradication From Serbian Autochthonous Plum Genotypes Using Cryotechniques (RS-6062279)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200215 (Fruit Research Institute, Čačak) (RS-200215)

DOI: 10.46793/SBT28.283J

ISBN: 978-86-87611-88-7

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URI
https://refri.institut-cacak.org/handle/123456789/499
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  • Radovi istraživača / Researchers’ publications
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Institut za voćarstvo

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