Show simple item record

dc.creatorKolarević, Tijana
dc.creatorMilincić, Danijel D.
dc.creatorVujović, Tatjana
dc.creatorGašić, Uroš M.
dc.creatorProkić, Ljiljana
dc.creatorKostić, Aleksandar Z.
dc.creatorCerović, Radosav
dc.creatorStanojević, Slađana P.
dc.creatorTesić, Živoslav Lj.
dc.creatorPesić, Mirjana B.
dc.date.accessioned2023-04-11T12:49:23Z
dc.date.available2023-04-11T12:49:23Z
dc.date.issued2021
dc.identifier.issn2311-7524
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/452
dc.description.abstractThe aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry 'Cacanska Bestrna' and blueberry 'Toro'. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA(3) ('Cacanska Bestrna') or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA ('Toro'). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D ('Cacanska Bestrna') or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA ('Toro'). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS(& BULL;+) and DPPH & BULL; scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS(& BULL;+) and ten to seventeen times lower DPPH & BULL; scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.en
dc.publisherBasel : MDPI
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200116/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200215/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceHorticulturae
dc.subjectVaccinium corymbosum L.en
dc.subjecttissue culture plantsen
dc.subjectRubus subg. Rubus Watsonen
dc.subjectradical scavenging activitiesen
dc.subjectphenolicsen
dc.subjectopen-field plantsen
dc.subjectin vitro callus cultureen
dc.subjectferric reducing poweren
dc.titlePhenolic compounds and antioxidant properties of field-grown and in vitro leaves, and calluses in blackberry and blueberryen
dc.typearticle
dc.rights.licenseBY
dc.citation.issue11
dc.citation.other7(11): -
dc.citation.rankM21
dc.citation.volume7
dc.identifier.doi10.3390/horticulturae7110420
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/160/449.pdf
dc.identifier.rcubconv_1635
dc.identifier.scopus2-s2.0-85118184182
dc.identifier.wos000726220100001
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record