Investigation of S-incompatibility breakdown in 'Karina' sweet cherry
Abstract
The paper presents results of a two-year study of self-(in)compatibility in 'Karina', previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of 'Karina' were selfed and pollinated with the nominally cross-incompatible cultivars 'Germersdorfer 1' and 'Lambert' at the beginning of full flowering. In the same way, the pollen of 'Karina' was used to pollinate 'Germersdorfer 1' and 'Lambert'. PCR analysis to determine S-genotypes of 'Karina', 'Germersdorfer 1' and 'Lambert' using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, 'Karina' behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with 'Germersdorfer 1' and 'Lambert'. In contrast, 'Germersdorfer 1' and 'Lambert' did not set ...any fruit when pollinated with 'Karina'. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of 'Karina' after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis.
Keywords:
S-genotype / Prunus avium L. / pollination / incompatible pollen tubes / fruit setSource:
Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future, 2021, 1308, 53-60Publisher:
- International Society for Horticultural Science, Leuven 1
Funding / projects:
- Development and preservation of genetic potential of temperate zone fruits (RS-MESTD-Technological Development (TD or TR)-31064)
DOI: 10.17660/ActaHortic.2021.1308.9
ISSN: 0567-7572
WoS: 000711941600009
Scopus: 2-s2.0-85105093567
Collections
Institution/Community
Institut za voćarstvoTY - CONF AU - Radičević, Sanja AU - Marić, Slađana AU - Cerović, Radosav AU - Đorđević, Milena PY - 2021 UR - https://refri.institut-cacak.org/handle/123456789/447 AB - The paper presents results of a two-year study of self-(in)compatibility in 'Karina', previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of 'Karina' were selfed and pollinated with the nominally cross-incompatible cultivars 'Germersdorfer 1' and 'Lambert' at the beginning of full flowering. In the same way, the pollen of 'Karina' was used to pollinate 'Germersdorfer 1' and 'Lambert'. PCR analysis to determine S-genotypes of 'Karina', 'Germersdorfer 1' and 'Lambert' using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, 'Karina' behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with 'Germersdorfer 1' and 'Lambert'. In contrast, 'Germersdorfer 1' and 'Lambert' did not set any fruit when pollinated with 'Karina'. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of 'Karina' after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis. PB - International Society for Horticultural Science, Leuven 1 C3 - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future T1 - Investigation of S-incompatibility breakdown in 'Karina' sweet cherry EP - 60 SP - 53 VL - 1308 DO - 10.17660/ActaHortic.2021.1308.9 UR - conv_1624 ER -
@conference{ author = "Radičević, Sanja and Marić, Slađana and Cerović, Radosav and Đorđević, Milena", year = "2021", abstract = "The paper presents results of a two-year study of self-(in)compatibility in 'Karina', previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of 'Karina' were selfed and pollinated with the nominally cross-incompatible cultivars 'Germersdorfer 1' and 'Lambert' at the beginning of full flowering. In the same way, the pollen of 'Karina' was used to pollinate 'Germersdorfer 1' and 'Lambert'. PCR analysis to determine S-genotypes of 'Karina', 'Germersdorfer 1' and 'Lambert' using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, 'Karina' behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with 'Germersdorfer 1' and 'Lambert'. In contrast, 'Germersdorfer 1' and 'Lambert' did not set any fruit when pollinated with 'Karina'. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of 'Karina' after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis.", publisher = "International Society for Horticultural Science, Leuven 1", journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future", title = "Investigation of S-incompatibility breakdown in 'Karina' sweet cherry", pages = "60-53", volume = "1308", doi = "10.17660/ActaHortic.2021.1308.9", url = "conv_1624" }
Radičević, S., Marić, S., Cerović, R.,& Đorđević, M.. (2021). Investigation of S-incompatibility breakdown in 'Karina' sweet cherry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future International Society for Horticultural Science, Leuven 1., 1308, 53-60. https://doi.org/10.17660/ActaHortic.2021.1308.9 conv_1624
Radičević S, Marić S, Cerović R, Đorđević M. Investigation of S-incompatibility breakdown in 'Karina' sweet cherry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future. 2021;1308:53-60. doi:10.17660/ActaHortic.2021.1308.9 conv_1624 .
Radičević, Sanja, Marić, Slađana, Cerović, Radosav, Đorđević, Milena, "Investigation of S-incompatibility breakdown in 'Karina' sweet cherry" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future, 1308 (2021):53-60, https://doi.org/10.17660/ActaHortic.2021.1308.9 ., conv_1624 .