In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes
Abstract
Autochthonous plum genotypes have immeasurable genetic and cultural value in Serbia. The paper deals with initial steps for establishment of an efficient protocol for micropropagation of selected eight autochthonous plum genotypes. The clonal selection has been carried out within cultivars 'Crvena Ranka' (six genotypes), 'Crnošljiva' (one genotype) and 'Metlaš' (one genotype) originated from heterogeneous local populations in western Serbia. Two surface-sterilizing procedures containing sodium hypochlorite or mercuric chloride as the main sterilizing agents, were tested for establishment of aseptic cultures. Single-node cuttings were cultured on Murashige and Skoog (MS) medium containing 2 mg L-1 BA, 0.5 mg L-1 IBA and 0.1 mg L-1 GA3 for rosette initiation. After four weeks of culture, frequency of explants with and without developed leaf rosettes and as well as contaminated and necrotic explants after each sterilization procedure were recorded. Significantly better results for contami...nation control and rosette initiation were achieved when surface sterilization was done with mercuric chloride in comparison to sodium hypochlorite in all genotypes, except 'Metlaš'. All genotypes can be successfully multiplied for at least five subcultures on MS medium with 1 mg L-1 BA, 0.1 mg L-1 NAA and 0.1 mg L-1 GA3. For most of the genotypes, constant increase in shoot formation capacity was observed over repeated subculturing. Further research is necessary to evaluate rooting and acclimatization stages in the micropropagation protocol of selected genotypes.
Keywords:
Subculturing / Prunus domestica L. / Multiplication capacity in vitro / Aseptic cultureSource:
Acta Horticulturae, 2021, 1322, 179-185Publisher:
- International Society for Horticultural Science
Funding / projects:
- This study was funded by the Ministry of Education, Science and Technological Developmentof the Republicof Serbia
Collections
Institution/Community
Institut za voćarstvoTY - CONF AU - Vujović, Tatjana AU - Jevremović, Darko AU - Glišić, Ivana AU - Milošević, Nebojša AU - Anđelić, Tatjana PY - 2021 UR - https://refri.institut-cacak.org/handle/123456789/438 AB - Autochthonous plum genotypes have immeasurable genetic and cultural value in Serbia. The paper deals with initial steps for establishment of an efficient protocol for micropropagation of selected eight autochthonous plum genotypes. The clonal selection has been carried out within cultivars 'Crvena Ranka' (six genotypes), 'Crnošljiva' (one genotype) and 'Metlaš' (one genotype) originated from heterogeneous local populations in western Serbia. Two surface-sterilizing procedures containing sodium hypochlorite or mercuric chloride as the main sterilizing agents, were tested for establishment of aseptic cultures. Single-node cuttings were cultured on Murashige and Skoog (MS) medium containing 2 mg L-1 BA, 0.5 mg L-1 IBA and 0.1 mg L-1 GA3 for rosette initiation. After four weeks of culture, frequency of explants with and without developed leaf rosettes and as well as contaminated and necrotic explants after each sterilization procedure were recorded. Significantly better results for contamination control and rosette initiation were achieved when surface sterilization was done with mercuric chloride in comparison to sodium hypochlorite in all genotypes, except 'Metlaš'. All genotypes can be successfully multiplied for at least five subcultures on MS medium with 1 mg L-1 BA, 0.1 mg L-1 NAA and 0.1 mg L-1 GA3. For most of the genotypes, constant increase in shoot formation capacity was observed over repeated subculturing. Further research is necessary to evaluate rooting and acclimatization stages in the micropropagation protocol of selected genotypes. PB - International Society for Horticultural Science C3 - Acta Horticulturae T1 - In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes EP - 185 SP - 179 VL - 1322 DO - 10.17660/ActaHortic.2021.1322.26 UR - conv_1663 ER -
@conference{ author = "Vujović, Tatjana and Jevremović, Darko and Glišić, Ivana and Milošević, Nebojša and Anđelić, Tatjana", year = "2021", abstract = "Autochthonous plum genotypes have immeasurable genetic and cultural value in Serbia. The paper deals with initial steps for establishment of an efficient protocol for micropropagation of selected eight autochthonous plum genotypes. The clonal selection has been carried out within cultivars 'Crvena Ranka' (six genotypes), 'Crnošljiva' (one genotype) and 'Metlaš' (one genotype) originated from heterogeneous local populations in western Serbia. Two surface-sterilizing procedures containing sodium hypochlorite or mercuric chloride as the main sterilizing agents, were tested for establishment of aseptic cultures. Single-node cuttings were cultured on Murashige and Skoog (MS) medium containing 2 mg L-1 BA, 0.5 mg L-1 IBA and 0.1 mg L-1 GA3 for rosette initiation. After four weeks of culture, frequency of explants with and without developed leaf rosettes and as well as contaminated and necrotic explants after each sterilization procedure were recorded. Significantly better results for contamination control and rosette initiation were achieved when surface sterilization was done with mercuric chloride in comparison to sodium hypochlorite in all genotypes, except 'Metlaš'. All genotypes can be successfully multiplied for at least five subcultures on MS medium with 1 mg L-1 BA, 0.1 mg L-1 NAA and 0.1 mg L-1 GA3. For most of the genotypes, constant increase in shoot formation capacity was observed over repeated subculturing. Further research is necessary to evaluate rooting and acclimatization stages in the micropropagation protocol of selected genotypes.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes", pages = "185-179", volume = "1322", doi = "10.17660/ActaHortic.2021.1322.26", url = "conv_1663" }
Vujović, T., Jevremović, D., Glišić, I., Milošević, N.,& Anđelić, T.. (2021). In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes. in Acta Horticulturae International Society for Horticultural Science., 1322, 179-185. https://doi.org/10.17660/ActaHortic.2021.1322.26 conv_1663
Vujović T, Jevremović D, Glišić I, Milošević N, Anđelić T. In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes. in Acta Horticulturae. 2021;1322:179-185. doi:10.17660/ActaHortic.2021.1322.26 conv_1663 .
Vujović, Tatjana, Jevremović, Darko, Glišić, Ivana, Milošević, Nebojša, Anđelić, Tatjana, "In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes" in Acta Horticulturae, 1322 (2021):179-185, https://doi.org/10.17660/ActaHortic.2021.1322.26 ., conv_1663 .