Morphological and molecular identification of Eutypa lata on grapevine in Serbia
Samo za registrovane korisnike
2019
Autori
Živković, SanjaVasić, Tanja
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Ivanović, Milan
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Jevremović, Darko
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Marković, Jordan
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Trkulja, Vojislav
Članak u časopisu (Objavljena verzija)
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions.... Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.
Ključne reči:
Vitis vinifera L / PCR / Eutypa lata / DiebackIzvor:
Journal of Plant Diseases and Protection, 2019, 126, 5, 479-483Izdavač:
- Springer Heidelberg, Heidelberg
DOI: 10.1007/s41348-019-00238-4
ISSN: 1861-3829
WoS: 000491548600014
Scopus: 2-s2.0-85068128514
Institucija/grupa
Institut za voćarstvoTY - JOUR AU - Živković, Sanja AU - Vasić, Tanja AU - Ivanović, Milan AU - Jevremović, Darko AU - Marković, Jordan AU - Trkulja, Vojislav PY - 2019 UR - https://refri.institut-cacak.org/handle/123456789/409 AB - During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata. PB - Springer Heidelberg, Heidelberg T2 - Journal of Plant Diseases and Protection T1 - Morphological and molecular identification of Eutypa lata on grapevine in Serbia EP - 483 IS - 5 SP - 479 VL - 126 DO - 10.1007/s41348-019-00238-4 UR - conv_1595 ER -
@article{ author = "Živković, Sanja and Vasić, Tanja and Ivanović, Milan and Jevremović, Darko and Marković, Jordan and Trkulja, Vojislav", year = "2019", abstract = "During a survey from 2004 to 2016, symptoms of grapevine dieback were observed in six vineyards in Serbia. Symptoms initially appeared as small, chlorotic, and necrotic spots along the rim of the leaves, deformation of leafs, and the appearance of shortened shoots, often with the so-called zigzag internodes. Over time, partial or complete dying of the vines developed. Symptomatic samples were collected and submitted to laboratory analysis. Based on the morphological characterization, the isolated fungus was initially identified as belonging to Eutypa species. Pathogenicity tests showed that 47 selected isolates caused tissue necrosis around the site of inoculation, chlorosis, and deformation of the leaves, along with the appearance of tiny, necrotic spots on the periphery of the leaf that fall off over time. Inoculated cuttings were stunted, dwarf-like with zigzag internodes. Molecular identification was done with sequence and phylogenetic analysis of ITS, TUB, and RPB2 genomic regions. Based on the phylogenetic analysis, all isolated fungi were determined as Eutypa lata.", publisher = "Springer Heidelberg, Heidelberg", journal = "Journal of Plant Diseases and Protection", title = "Morphological and molecular identification of Eutypa lata on grapevine in Serbia", pages = "483-479", number = "5", volume = "126", doi = "10.1007/s41348-019-00238-4", url = "conv_1595" }
Živković, S., Vasić, T., Ivanović, M., Jevremović, D., Marković, J.,& Trkulja, V.. (2019). Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection Springer Heidelberg, Heidelberg., 126(5), 479-483. https://doi.org/10.1007/s41348-019-00238-4 conv_1595
Živković S, Vasić T, Ivanović M, Jevremović D, Marković J, Trkulja V. Morphological and molecular identification of Eutypa lata on grapevine in Serbia. in Journal of Plant Diseases and Protection. 2019;126(5):479-483. doi:10.1007/s41348-019-00238-4 conv_1595 .
Živković, Sanja, Vasić, Tanja, Ivanović, Milan, Jevremović, Darko, Marković, Jordan, Trkulja, Vojislav, "Morphological and molecular identification of Eutypa lata on grapevine in Serbia" in Journal of Plant Diseases and Protection, 126, no. 5 (2019):479-483, https://doi.org/10.1007/s41348-019-00238-4 ., conv_1595 .