The polymorphism of ACS1 and ACO1 genes in some Malus species
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2019
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The allelic polymorphism of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase that catalyze the last two reactions in the ethylene biosynthetic pathway, were analyzed in the following nine Malus species from the ex situ collection of the Fruit Research Institute (FRI), Cacak, Republic of Serbia, i.e., Malus x atrosanguinea (Spaeth) Schneid., Malus floribunda Siebold ex Van Houtte, Malus hupehensis (Pamp.) Rehder, Malus x micromalus Makino, Malus niedzwetzkyana Dieck, Malus prunifolia (Willd.) Borkh. var. microcarpa, Malus prunifolia (Willd.) Borkh. var. xanthocarpa, Malus sargentii Rehder and Malus sieboldii (Regel) Rehder. The alleles were revealed by polymerase chain reaction (PCR) for ACS1 gene and additional restriction analysis of PCR product for ACO1 gene with two restriction enzymes (BamH1 and RsaI). The PCR product of 490 bp corresponded to the ACS1-1 allele and homozygosity of this allele was identified in all of the assessed Malus species. The comparison of polym...orphisms observed upon the restriction analysis [BamH1 - two segregating fragments of 700 bp (f(1) fragment) and 760 bp (f(2) fragment); RsaI - three segregating fragments of 530 and 680 bp (together considered as f(1) fragment), 1,270 bp (f(2) fragment) and 1,050 bp (f(3) fragment)] resulted in the identification of four alleles of ACO1 gene - a, b, c and d, generating five genotypes (dd - four species, ac - one species, ad - two species, bd - one species and cd - one species). This study confirmed that allele d of the ACO1 gene has been detected only in the Malus wild species so far.
Ključne reči:
wild species / ethylene / apple / allele / ACC synthase / ACC oxidaseIzvor:
III International Symposium on Horticultural Crop Wild Relatives, 2019, 1259, 13-18Izdavač:
- International Society for Horticultural Science, Leuven 1
Finansiranje / projekti:
- Stvaranje i očuvanje genetičkog potencijala kontinentalnih vrsta voćaka (RS-MESTD-Technological Development (TD or TR)-31064)
DOI: 10.17660/ActaHortic.2019.1259.3
ISSN: 0567-7572
WoS: 000712368500003
Scopus: 2-s2.0-85076395213
Institucija/grupa
Institut za voćarstvoTY - CONF AU - Marić, Slađana AU - Radičević, Sanja AU - Milošević, Nebojša AU - Lukić, Milan AU - Đorđević, Milena PY - 2019 UR - https://refri.institut-cacak.org/handle/123456789/397 AB - The allelic polymorphism of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase that catalyze the last two reactions in the ethylene biosynthetic pathway, were analyzed in the following nine Malus species from the ex situ collection of the Fruit Research Institute (FRI), Cacak, Republic of Serbia, i.e., Malus x atrosanguinea (Spaeth) Schneid., Malus floribunda Siebold ex Van Houtte, Malus hupehensis (Pamp.) Rehder, Malus x micromalus Makino, Malus niedzwetzkyana Dieck, Malus prunifolia (Willd.) Borkh. var. microcarpa, Malus prunifolia (Willd.) Borkh. var. xanthocarpa, Malus sargentii Rehder and Malus sieboldii (Regel) Rehder. The alleles were revealed by polymerase chain reaction (PCR) for ACS1 gene and additional restriction analysis of PCR product for ACO1 gene with two restriction enzymes (BamH1 and RsaI). The PCR product of 490 bp corresponded to the ACS1-1 allele and homozygosity of this allele was identified in all of the assessed Malus species. The comparison of polymorphisms observed upon the restriction analysis [BamH1 - two segregating fragments of 700 bp (f(1) fragment) and 760 bp (f(2) fragment); RsaI - three segregating fragments of 530 and 680 bp (together considered as f(1) fragment), 1,270 bp (f(2) fragment) and 1,050 bp (f(3) fragment)] resulted in the identification of four alleles of ACO1 gene - a, b, c and d, generating five genotypes (dd - four species, ac - one species, ad - two species, bd - one species and cd - one species). This study confirmed that allele d of the ACO1 gene has been detected only in the Malus wild species so far. PB - International Society for Horticultural Science, Leuven 1 C3 - III International Symposium on Horticultural Crop Wild Relatives T1 - The polymorphism of ACS1 and ACO1 genes in some Malus species EP - 18 SP - 13 VL - 1259 DO - 10.17660/ActaHortic.2019.1259.3 UR - conv_1631 ER -
@conference{ author = "Marić, Slađana and Radičević, Sanja and Milošević, Nebojša and Lukić, Milan and Đorđević, Milena", year = "2019", abstract = "The allelic polymorphism of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase that catalyze the last two reactions in the ethylene biosynthetic pathway, were analyzed in the following nine Malus species from the ex situ collection of the Fruit Research Institute (FRI), Cacak, Republic of Serbia, i.e., Malus x atrosanguinea (Spaeth) Schneid., Malus floribunda Siebold ex Van Houtte, Malus hupehensis (Pamp.) Rehder, Malus x micromalus Makino, Malus niedzwetzkyana Dieck, Malus prunifolia (Willd.) Borkh. var. microcarpa, Malus prunifolia (Willd.) Borkh. var. xanthocarpa, Malus sargentii Rehder and Malus sieboldii (Regel) Rehder. The alleles were revealed by polymerase chain reaction (PCR) for ACS1 gene and additional restriction analysis of PCR product for ACO1 gene with two restriction enzymes (BamH1 and RsaI). The PCR product of 490 bp corresponded to the ACS1-1 allele and homozygosity of this allele was identified in all of the assessed Malus species. The comparison of polymorphisms observed upon the restriction analysis [BamH1 - two segregating fragments of 700 bp (f(1) fragment) and 760 bp (f(2) fragment); RsaI - three segregating fragments of 530 and 680 bp (together considered as f(1) fragment), 1,270 bp (f(2) fragment) and 1,050 bp (f(3) fragment)] resulted in the identification of four alleles of ACO1 gene - a, b, c and d, generating five genotypes (dd - four species, ac - one species, ad - two species, bd - one species and cd - one species). This study confirmed that allele d of the ACO1 gene has been detected only in the Malus wild species so far.", publisher = "International Society for Horticultural Science, Leuven 1", journal = "III International Symposium on Horticultural Crop Wild Relatives", title = "The polymorphism of ACS1 and ACO1 genes in some Malus species", pages = "18-13", volume = "1259", doi = "10.17660/ActaHortic.2019.1259.3", url = "conv_1631" }
Marić, S., Radičević, S., Milošević, N., Lukić, M.,& Đorđević, M.. (2019). The polymorphism of ACS1 and ACO1 genes in some Malus species. in III International Symposium on Horticultural Crop Wild Relatives International Society for Horticultural Science, Leuven 1., 1259, 13-18. https://doi.org/10.17660/ActaHortic.2019.1259.3 conv_1631
Marić S, Radičević S, Milošević N, Lukić M, Đorđević M. The polymorphism of ACS1 and ACO1 genes in some Malus species. in III International Symposium on Horticultural Crop Wild Relatives. 2019;1259:13-18. doi:10.17660/ActaHortic.2019.1259.3 conv_1631 .
Marić, Slađana, Radičević, Sanja, Milošević, Nebojša, Lukić, Milan, Đorđević, Milena, "The polymorphism of ACS1 and ACO1 genes in some Malus species" in III International Symposium on Horticultural Crop Wild Relatives, 1259 (2019):13-18, https://doi.org/10.17660/ActaHortic.2019.1259.3 ., conv_1631 .