Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis
2018
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Аутори
Vasilijević, BojanaKnezević-Vukcević, Jelena
Mitić-Culafić, Dragana
Orčić, Dejan
Francisković, Marina
Srdić-Rajić, Tatjana
Jovanović, Marina
Nikolić, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined cc-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g(-1)) and quinic acid (11.1 mg g-1) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC50 was 5.27 mu g mL(-1)), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC50 values were 69.4 mu g mL-1 and 120 mu g mL(-1) for EO, and 1.27 mg mL(-1) and 2.86 mg mL(-1) for PDW, respectively. PDW was genotoxic (0.3 mg mL(-1) and 1 mg mL(-1) in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL(-1)). In mixtures with doxorubicin... cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects.
Извор:
Food and Chemical Toxicology, 2018, 112, 118-125Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1016/j.fct.2017.12.044
ISSN: 0278-6915
PubMed: 29287791
WoS: 000425568900013
Scopus: 2-s2.0-85039849250
Институција/група
Institut za voćarstvoTY - JOUR AU - Vasilijević, Bojana AU - Knezević-Vukcević, Jelena AU - Mitić-Culafić, Dragana AU - Orčić, Dejan AU - Francisković, Marina AU - Srdić-Rajić, Tatjana AU - Jovanović, Marina AU - Nikolić, Biljana PY - 2018 UR - https://refri.institut-cacak.org/handle/123456789/379 AB - Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined cc-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g(-1)) and quinic acid (11.1 mg g-1) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC50 was 5.27 mu g mL(-1)), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC50 values were 69.4 mu g mL-1 and 120 mu g mL(-1) for EO, and 1.27 mg mL(-1) and 2.86 mg mL(-1) for PDW, respectively. PDW was genotoxic (0.3 mg mL(-1) and 1 mg mL(-1) in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL(-1)). In mixtures with doxorubicin cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis EP - 125 SP - 118 VL - 112 DO - 10.1016/j.fct.2017.12.044 UR - conv_1576 ER -
@article{ author = "Vasilijević, Bojana and Knezević-Vukcević, Jelena and Mitić-Culafić, Dragana and Orčić, Dejan and Francisković, Marina and Srdić-Rajić, Tatjana and Jovanović, Marina and Nikolić, Biljana", year = "2018", abstract = "Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined cc-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g(-1)) and quinic acid (11.1 mg g-1) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC50 was 5.27 mu g mL(-1)), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC50 values were 69.4 mu g mL-1 and 120 mu g mL(-1) for EO, and 1.27 mg mL(-1) and 2.86 mg mL(-1) for PDW, respectively. PDW was genotoxic (0.3 mg mL(-1) and 1 mg mL(-1) in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL(-1)). In mixtures with doxorubicin cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis", pages = "125-118", volume = "112", doi = "10.1016/j.fct.2017.12.044", url = "conv_1576" }
Vasilijević, B., Knezević-Vukcević, J., Mitić-Culafić, D., Orčić, D., Francisković, M., Srdić-Rajić, T., Jovanović, M.,& Nikolić, B.. (2018). Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 112, 118-125. https://doi.org/10.1016/j.fct.2017.12.044 conv_1576
Vasilijević B, Knezević-Vukcević J, Mitić-Culafić D, Orčić D, Francisković M, Srdić-Rajić T, Jovanović M, Nikolić B. Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis. in Food and Chemical Toxicology. 2018;112:118-125. doi:10.1016/j.fct.2017.12.044 conv_1576 .
Vasilijević, Bojana, Knezević-Vukcević, Jelena, Mitić-Culafić, Dragana, Orčić, Dejan, Francisković, Marina, Srdić-Rajić, Tatjana, Jovanović, Marina, Nikolić, Biljana, "Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis" in Food and Chemical Toxicology, 112 (2018):118-125, https://doi.org/10.1016/j.fct.2017.12.044 ., conv_1576 .