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Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis

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2018
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Authors
Vasilijević, Bojana
Knezević-Vukcević, Jelena
Mitić-Culafić, Dragana
Orčić, Dejan
Francisković, Marina
Srdić-Rajić, Tatjana
Jovanović, Marina
Nikolić, Biljana
Article (Published version)
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Abstract
Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined cc-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g(-1)) and quinic acid (11.1 mg g-1) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC50 was 5.27 mu g mL(-1)), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC50 values were 69.4 mu g mL-1 and 120 mu g mL(-1) for EO, and 1.27 mg mL(-1) and 2.86 mg mL(-1) for PDW, respectively. PDW was genotoxic (0.3 mg mL(-1) and 1 mg mL(-1) in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL(-1)). In mixtures with doxorubicin... cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects.

Source:
Food and Chemical Toxicology, 2018, 112, 118-125
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-172058)

DOI: 10.1016/j.fct.2017.12.044

ISSN: 0278-6915

PubMed: 29287791

WoS: 000425568900013

Scopus: 2-s2.0-85039849250
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URI
https://refri.institut-cacak.org/handle/123456789/379
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Institut za voćarstvo

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