Prisustvo virusa američke visokožbunaste borovnice (Vaccinium corymbosum L.) u Srbiji

Abstract

Ispitivanje prisustva virusa američke visokožbunaste borovnice (Vaccinium corymbosum L.) u Srbiji sprovedeno je u periodu 2011-2015. godine. Sakupljen je i analiziran 81 uzorak lišća borovnice iz 15 lokaliteta na prisustvo 8 virusa. Serološki ELISA test je primenjen za ispitivanje uzoraka na prisustvo: virusa sprženosti borovnice (Blueberryscorch virus - BlScV), virusa šoka borovnice (Blueberry shock virus - BlShV), virusa nitavosti lišća borovnice (Blueberry shoestring virus - BSSV), virusa šarenila lista borovnice (Blueberry leaf mottle virus - BLMoV), virusa prstenaste pegavosti duvana (Tobacco ringspot virus - TRSV) i virusa prstenaste pegavosti paradajza (Tomato ringspot virus - ToRSV). Na prisustvo virusa crvene prstenaste pegavosti borovnice (Blueberry red ringspot virus - BRRV) uzorci su analizirani PCR metodom, a na prisustvo virusa mozaika borovnice (Blueberry mosaic-associated virus - BlMaV) RT-PCR metodom. Rezultati ispitivanja su potvrdili prisustvo BlMaV u 8 (9,9%) i BRRV u 1 (1,2%) uzorku. Prisustvo BlScV, BlShV, BLMoV, BSSV, TRSV i ToRSV nije dokazano ni u jednom ispitivanom uzorku.

A large-scale survey for highbush blueberry (Vaccinium corymbosum L.) viruses in Serbia was performed from 2011 to 2015. A total of 81 leaf samples from 15 locations were collected and analyzed for the presence of 8 viruses. Serological ELISA assay was performed to determine the presence of: Blueberry scorch virus (BlScV), Blueberry shock virus (BlShV), Blueberry shoestring virus (BSSV), Blueberry leaf mottle virus (BLMoV), Tobacco ringspot virus (TRSV) and Tomato ringspot virus (ToRSV). All samples were tested for the presence of Blueberry red ringspot virus (BRRV) by PCR and for Blueberry mosaic-associated virus (BlMaV) by RT-PCR test. The analyses confirmed the presence of BlMaV in 8 (9.9%) samples and BRRV in 1 (1.2%) sample. No BlScV, BlShV, BLMoV, BSSV, TRSV or ToRSV viruses were detected in any of the analyzed samples.