Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
Abstract
In vitro shoot tips of Serbian autochthonous plums 'Sitnica' (Prunus domestica L.) and 'Crvena Ranka' (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum 'Sitnica', with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading tr...eatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. 'Crvena Ranka' exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3-81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.
Keywords:
vitrification solution / Prunus insititia L. / Prunus domestica L. / in vitro shoot tips / dehydration / cryopreservationSource:
Biologia, 2015, 70, 10, 1359-1365Publisher:
- Springer, New York
Funding / projects:
DOI: 10.1515/biolog-2015-0162
ISSN: 0006-3088
WoS: 000365800900010
Scopus: 2-s2.0-84950104664
Collections
Institution/Community
Institut za voćarstvoTY - JOUR AU - Vujović, Tatjana AU - Ružić, Đurđina AU - Cerović, Radosav PY - 2015 UR - https://refri.institut-cacak.org/handle/123456789/338 AB - In vitro shoot tips of Serbian autochthonous plums 'Sitnica' (Prunus domestica L.) and 'Crvena Ranka' (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum 'Sitnica', with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. 'Crvena Ranka' exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3-81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation. PB - Springer, New York T2 - Biologia T1 - Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification EP - 1365 IS - 10 SP - 1359 VL - 70 DO - 10.1515/biolog-2015-0162 UR - conv_1537 ER -
@article{ author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav", year = "2015", abstract = "In vitro shoot tips of Serbian autochthonous plums 'Sitnica' (Prunus domestica L.) and 'Crvena Ranka' (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum 'Sitnica', with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. 'Crvena Ranka' exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3-81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.", publisher = "Springer, New York", journal = "Biologia", title = "Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification", pages = "1365-1359", number = "10", volume = "70", doi = "10.1515/biolog-2015-0162", url = "conv_1537" }
Vujović, T., Ružić, Đ.,& Cerović, R.. (2015). Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification. in Biologia Springer, New York., 70(10), 1359-1365. https://doi.org/10.1515/biolog-2015-0162 conv_1537
Vujović T, Ružić Đ, Cerović R. Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification. in Biologia. 2015;70(10):1359-1365. doi:10.1515/biolog-2015-0162 conv_1537 .
Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification" in Biologia, 70, no. 10 (2015):1359-1365, https://doi.org/10.1515/biolog-2015-0162 ., conv_1537 .