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Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure

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Authors
Ružić, Đurđina
Vujović, Tatjana
Cerović, Radosav
Article (Published version)
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Abstract
In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the P...VS A3-based vitrification technique for a long-term storage of this genotype.

Keywords:
vitrification solutions / Prunus cerasus x Prunus canescens / liquid nitrogen
Source:
Horticultural Science, 2014, 41, 2, 55-63
Publisher:
  • Czech Academy Agricultural Sciences, Prague

DOI: 10.17221/234/2013-HORTSCI

ISSN: 0862-867X

WoS: 000338096300002

Scopus: 2-s2.0-84902440320
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URI
https://refri.institut-cacak.org/handle/123456789/326
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Institut za voćarstvo

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