Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.)
Апстракт
The allelic polymorphism and inheritance of MdACS1 and MdACO1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: Golden Delicious'xJames Grieve', Golden Delicious'xJonathan', Cox's Orange Pippin'xGolden Delicious', Cox's Orange Pippin'xJonathan'. Polymorphisms were detected by PCR and the use of two restriction enzymes (BamH1 and RsaI). In addition, new primers were designed for the further discrimination of MdACO1 alleles. Two alleles of MdACS1 gene (MdACS1-1 and MdACS1-2) and three alleles of MdACO1 gene (a, b and c) were detected. Cloning and sequencing of MdACO1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a, b and c belonged to the MdACO1 gene. It is apparent that polymorphisms within the MdACS1 and MdACO1 genes could aid cultivar genotyping and identification and, furth...ermore, that the MdACS1-2/2 genotype is correlated with a long apple storage life.
Кључне речи:
fruit ripening / ethylene / apple / 1-aminocyclopropane-1-carboxylate synthase gene / 1-aminocyclopropane-1-carboxylate oxidase geneИзвор:
Plant Breeding, 2014, 133, 1, 108-114Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Стварање и очување генетичког потенцијала континенталних врста воћака (RS-MESTD-Technological Development (TD or TR)-31064)
DOI: 10.1111/pbr.12124
ISSN: 0179-9541
WoS: 000330800600015
Scopus: 2-s2.0-84892481797
Институција/група
Institut za voćarstvoTY - JOUR AU - Marić, Slađana AU - Lukić, Milan PY - 2014 UR - https://refri.institut-cacak.org/handle/123456789/321 AB - The allelic polymorphism and inheritance of MdACS1 and MdACO1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: Golden Delicious'xJames Grieve', Golden Delicious'xJonathan', Cox's Orange Pippin'xGolden Delicious', Cox's Orange Pippin'xJonathan'. Polymorphisms were detected by PCR and the use of two restriction enzymes (BamH1 and RsaI). In addition, new primers were designed for the further discrimination of MdACO1 alleles. Two alleles of MdACS1 gene (MdACS1-1 and MdACS1-2) and three alleles of MdACO1 gene (a, b and c) were detected. Cloning and sequencing of MdACO1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a, b and c belonged to the MdACO1 gene. It is apparent that polymorphisms within the MdACS1 and MdACO1 genes could aid cultivar genotyping and identification and, furthermore, that the MdACS1-2/2 genotype is correlated with a long apple storage life. PB - Wiley, Hoboken T2 - Plant Breeding T1 - Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.) EP - 114 IS - 1 SP - 108 VL - 133 DO - 10.1111/pbr.12124 UR - conv_1515 ER -
@article{ author = "Marić, Slađana and Lukić, Milan", year = "2014", abstract = "The allelic polymorphism and inheritance of MdACS1 and MdACO1 genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: Golden Delicious'xJames Grieve', Golden Delicious'xJonathan', Cox's Orange Pippin'xGolden Delicious', Cox's Orange Pippin'xJonathan'. Polymorphisms were detected by PCR and the use of two restriction enzymes (BamH1 and RsaI). In addition, new primers were designed for the further discrimination of MdACO1 alleles. Two alleles of MdACS1 gene (MdACS1-1 and MdACS1-2) and three alleles of MdACO1 gene (a, b and c) were detected. Cloning and sequencing of MdACO1 alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles a, b and c belonged to the MdACO1 gene. It is apparent that polymorphisms within the MdACS1 and MdACO1 genes could aid cultivar genotyping and identification and, furthermore, that the MdACS1-2/2 genotype is correlated with a long apple storage life.", publisher = "Wiley, Hoboken", journal = "Plant Breeding", title = "Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.)", pages = "114-108", number = "1", volume = "133", doi = "10.1111/pbr.12124", url = "conv_1515" }
Marić, S.,& Lukić, M.. (2014). Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.). in Plant Breeding Wiley, Hoboken., 133(1), 108-114. https://doi.org/10.1111/pbr.12124 conv_1515
Marić S, Lukić M. Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.). in Plant Breeding. 2014;133(1):108-114. doi:10.1111/pbr.12124 conv_1515 .
Marić, Slađana, Lukić, Milan, "Allelic polymorphism and inheritance of MdACS1 and MdAC01 genes in apple (Malus x domestica Borkh.)" in Plant Breeding, 133, no. 1 (2014):108-114, https://doi.org/10.1111/pbr.12124 ., conv_1515 .