Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică
Effect of jasmonic acid on in vitro multiplication of low vigorous Pear and cherry rootstocks
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The capacity of Jasmonic acid (JA) to improve in vitro multiplication phase in micropropagated shoots of low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6 respectively, was studied. The experiment was performed during the multiplication phase and it included 23 media types containing Murashige and Skoog (1962) macro, micro salts and organic complex supplemented with JA at four concentrations (1, 2.3, 5 and 10 μM) alone and in combination with benzyl adenine - BA (4.4 μM), indole-3-butyric acid - IBA (1, 2.30, 2.46, 5 and 10 μM), a-napthyl acetic acid -NAA (1, 2.30, 2.68, 5, 10 μM), and indole-3-acetic acid - IAA (1, 2.30, 2.85, 5, 10 μM). The multiplication parameters, such as multiplication index, length of axial and lateral shoots, FW and DW of shoots as well as quality of multiplied shoots were monitored. The highest multiplication rate in both genotypes was obtained on medium with BA (4.4 μM) in combination with IBA (1 and 2.3 μM). The longest axial and lateral shoot...s were also obtained on media with BA in combination with JA or other three auxins used. The main characteristics of the JA effect (used alone) were big shoots, large green leaves, small quantity of firm, nodular callus as well as occurrence of rooting. The obtained results suggest that JA used alone should not be used to improve the multiplication process, but only in combination with BA and IBA or NAA for the high quality plantlets which is important for the rooting phase, to skip elongation phase.
Keywords:
Rootstocks / Multiplication / Jasmonic acid / In vitro / portaltoi / acid Jasmonic / in vitro / multimplicareSource:
Fruit Growing Research, 2013, 29, 1, 106-112Publisher:
- Research Institute for Fruit Growing
Funding / projects:
- Development and preservation of genetic potential of temperate zone fruits (RS-31064)
- Stvaranje i proučavanje novih genotipova voćaka i uvođenje savremenih biotehnologija gajenja i prerade voća (RS-20013)
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Institut za voćarstvoTY - JOUR AU - Ružić, Đurđina AU - Vujović, Tatjana AU - Cerović, Radosav PY - 2013 UR - https://refri.institut-cacak.org/handle/123456789/292 AB - The capacity of Jasmonic acid (JA) to improve in vitro multiplication phase in micropropagated shoots of low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6 respectively, was studied. The experiment was performed during the multiplication phase and it included 23 media types containing Murashige and Skoog (1962) macro, micro salts and organic complex supplemented with JA at four concentrations (1, 2.3, 5 and 10 μM) alone and in combination with benzyl adenine - BA (4.4 μM), indole-3-butyric acid - IBA (1, 2.30, 2.46, 5 and 10 μM), a-napthyl acetic acid -NAA (1, 2.30, 2.68, 5, 10 μM), and indole-3-acetic acid - IAA (1, 2.30, 2.85, 5, 10 μM). The multiplication parameters, such as multiplication index, length of axial and lateral shoots, FW and DW of shoots as well as quality of multiplied shoots were monitored. The highest multiplication rate in both genotypes was obtained on medium with BA (4.4 μM) in combination with IBA (1 and 2.3 μM). The longest axial and lateral shoots were also obtained on media with BA in combination with JA or other three auxins used. The main characteristics of the JA effect (used alone) were big shoots, large green leaves, small quantity of firm, nodular callus as well as occurrence of rooting. The obtained results suggest that JA used alone should not be used to improve the multiplication process, but only in combination with BA and IBA or NAA for the high quality plantlets which is important for the rooting phase, to skip elongation phase. PB - Research Institute for Fruit Growing T2 - Fruit Growing Research T1 - Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică T1 - Effect of jasmonic acid on in vitro multiplication of low vigorous Pear and cherry rootstocks EP - 112 IS - 1 SP - 106 VL - 29 UR - conv_1716 ER -
@article{ author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav", year = "2013", abstract = "The capacity of Jasmonic acid (JA) to improve in vitro multiplication phase in micropropagated shoots of low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6 respectively, was studied. The experiment was performed during the multiplication phase and it included 23 media types containing Murashige and Skoog (1962) macro, micro salts and organic complex supplemented with JA at four concentrations (1, 2.3, 5 and 10 μM) alone and in combination with benzyl adenine - BA (4.4 μM), indole-3-butyric acid - IBA (1, 2.30, 2.46, 5 and 10 μM), a-napthyl acetic acid -NAA (1, 2.30, 2.68, 5, 10 μM), and indole-3-acetic acid - IAA (1, 2.30, 2.85, 5, 10 μM). The multiplication parameters, such as multiplication index, length of axial and lateral shoots, FW and DW of shoots as well as quality of multiplied shoots were monitored. The highest multiplication rate in both genotypes was obtained on medium with BA (4.4 μM) in combination with IBA (1 and 2.3 μM). The longest axial and lateral shoots were also obtained on media with BA in combination with JA or other three auxins used. The main characteristics of the JA effect (used alone) were big shoots, large green leaves, small quantity of firm, nodular callus as well as occurrence of rooting. The obtained results suggest that JA used alone should not be used to improve the multiplication process, but only in combination with BA and IBA or NAA for the high quality plantlets which is important for the rooting phase, to skip elongation phase.", publisher = "Research Institute for Fruit Growing", journal = "Fruit Growing Research", title = "Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică, Effect of jasmonic acid on in vitro multiplication of low vigorous Pear and cherry rootstocks", pages = "112-106", number = "1", volume = "29", url = "conv_1716" }
Ružić, Đ., Vujović, T.,& Cerović, R.. (2013). Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică. in Fruit Growing Research Research Institute for Fruit Growing., 29(1), 106-112. conv_1716
Ružić Đ, Vujović T, Cerović R. Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică. in Fruit Growing Research. 2013;29(1):106-112. conv_1716 .
Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică" in Fruit Growing Research, 29, no. 1 (2013):106-112, conv_1716 .