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Determination of S-genotype and ACS1-genotype in apple seedlings developed at Fruit Research Institute - Čačak

dc.creatorMarić, Slađana
dc.creatorLukić, Milan
dc.date.accessioned2023-04-11T12:33:53Z
dc.date.available2023-04-11T12:33:53Z
dc.date.issued2013
dc.identifier.issn1820-5054
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/282
dc.description.abstractU radu su predstavljeni rezultati determinacije S-genotipa i ACS1 -genotipa roditeljskih sorti i sejanaca jabuke dobijenih samooprašivanjem sorte Gala Must i potpuno kompatibilnim ukrštanjem sorti Fuji Kiku 8 x Topaz u Institutu za voćarstvo u Čačku. Aleli S-RNaze i ACS1 gena identifikovani su lančanom reakcijom polimeraze. Za roditeljske sorte utvrđeno je da su homozigotne za alel ACS1-2 (ACS1-2/ACS1-2), kao i da su sledećih S alelnih konstitucija: Gala Must - S2S5, Fuji Kiku 8 - S1S9 i Topaz - S2S5. Analiza sedam sejanaca dobijenih samooprašivanjem sorte Gala Must pokazala je da tri sejanca imaju alelnu konstituciju S2S2, dva S2S5 i dva S5S5, kao i da sejanci homozigotni za S5 alel ne pripadaju navedenom potomstvu. Od deset sejanaca dobijenih iz ukrštanja Fuji Kiku 8 (S1S9) x Topaz (S2S5), jedan sejanac ima konstituciju S2S2, tri S2S5, pet S2S9 i jedan S2Sx alelnu konstituciju. Determinacija ACS1-genotipa pokazala je da su svi ispitivani sejanci homozigotni za alel ACS1-2, izuzev sejanaca GMxGM-2 i GMxGM-6 za koje je utvrđeno da su heterozigoti (ACS1-1/ACS1-2).sr
dc.description.abstractThe paper presents the results of determination of S-genotype and ACS1 -genotype in parental cultivars and seedlings derived from the progenies obtained after self-pollination of apple cultivar 'Gala Must' and from the fully compatible cross 'Fuji Kiku 8' x 'Topaz' at Fruit Research Institute - Čacak. Alleles of S-RNase and ACS1 genes were identified by polymerase chain rection (PCR). The parental cultivars were homozygous for allele ACS1-2 (ACS1-2/ACS1-2), and the following S-genotypes were determined: 'Gala Must' - S2S5, 'Fuji Kiku 8' - S1S9 and 'Topaz' - S2S5. The analysis showed that the selfed progeny of 'Gala Must' segregated 3 S2S2 : 2 S2S5 : 2 S5S5, as well as that the seedlings homozygous for S5 allele do not belong to the selfed progeny. Of ten seedlings derived from the cross 'Fuji Kiku 8' (S2S9) x 'Topaz' (S2S5), one was S1S2, three were S1S5, five were S2S9 and one was S2Sx. Determination of ACS1 -genotype showed that evaluated seedlings were homozygous for allele ACS1-2, with two exceptions, i.e. GMxGM-2 and GMxGM-6. These seedlings were expected to be ACS1-2/ACS1-2, but are observed to be ACS1-1/ACS1-2 genotype.en
dc.publisherNaučno voćarsko društvo Srbije, Čačak
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31064/RS//
dc.rightsopenAccess
dc.sourceVoćarstvo
dc.subjectS-RNazasr
dc.subjectsejanacsr
dc.subjectMalus x domesticasr
dc.subjectACS1sr
dc.subjectS-RNaseen
dc.subjectseedlingen
dc.subjectMalus x domesticaen
dc.subjectACS1en
dc.titleDeterminacija S-genotipa i ACS1-genotipa sejanaca jabuke stvorenih u Institutu za voćarstvo - Čačaksr
dc.titleDetermination of S-genotype and ACS1-genotype in apple seedlings developed at Fruit Research Institute - Čačaken
dc.typearticle
dc.rights.licenseARR
dc.citation.epage86
dc.citation.issue183-184
dc.citation.other47(183-184): 79-86
dc.citation.rankM51
dc.citation.spage79
dc.citation.volume47
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/88/279.pdf
dc.identifier.rcubconv_1333
dc.type.versionpublishedVersion


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