Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.)
Abstract
Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' and 'Goldtraube'. All media contained 0.5 mg/L zeatin applied either alone or combined with 0.1, 1 and 5 mg/L IBA. In vitro rooting was induced using mAN medium supplemented with 0.8 mg/L IBA and 4 g/L activated charcoal. The results obtained showed that mAN medium is more suitable for in vitro multiplication of the selected highbush blueberry cultivars than MS medium. Low concentration of IBA ( LT = 1 mg/L) added in zeatin-supplemented mAN medium increases shoot multiplication efficiency of highbush blueberries in vitro and can be recommended for large-scale propagation of high quality plants. MS medium induced partial or full necrosis of stems and leaves, which was more pronounced on media containing zeatin combined with increasing concentration of IBA. Rooting capacity of shoots varied widely among the ...tested blueberry cultivars. The highest rooting and acclimatisation rates were achieved in 'Goldtraube' (82.8 and 91.8% respectively), and the lowest (10 and 66.7% respectively) were in 'Berkeley'.
Keywords:
rooting / nutritive media / multiplication / highbush blueberry / acclimatisationSource:
Acta Horticulturae, 2012, 926, 265-272Publisher:
- International Society for Horticultural Science
Funding / projects:
DOI: 10.17660/ActaHortic.2012.926.36
ISSN: 0567-7572
WoS: 000314966400036
Scopus: 2-s2.0-84863613774
Collections
Institution/Community
Institut za voćarstvoTY - CONF AU - Ružić, Đurđina AU - Vujović, Tatjana AU - Cerović, Radosav AU - Ostrolucka, M. G. AU - Gajdosova, Alena PY - 2012 UR - https://refri.institut-cacak.org/handle/123456789/263 AB - Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' and 'Goldtraube'. All media contained 0.5 mg/L zeatin applied either alone or combined with 0.1, 1 and 5 mg/L IBA. In vitro rooting was induced using mAN medium supplemented with 0.8 mg/L IBA and 4 g/L activated charcoal. The results obtained showed that mAN medium is more suitable for in vitro multiplication of the selected highbush blueberry cultivars than MS medium. Low concentration of IBA ( LT = 1 mg/L) added in zeatin-supplemented mAN medium increases shoot multiplication efficiency of highbush blueberries in vitro and can be recommended for large-scale propagation of high quality plants. MS medium induced partial or full necrosis of stems and leaves, which was more pronounced on media containing zeatin combined with increasing concentration of IBA. Rooting capacity of shoots varied widely among the tested blueberry cultivars. The highest rooting and acclimatisation rates were achieved in 'Goldtraube' (82.8 and 91.8% respectively), and the lowest (10 and 66.7% respectively) were in 'Berkeley'. PB - International Society for Horticultural Science C3 - Acta Horticulturae T1 - Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.) EP - 272 SP - 265 VL - 926 DO - 10.17660/ActaHortic.2012.926.36 UR - conv_1745 ER -
@conference{ author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav and Ostrolucka, M. G. and Gajdosova, Alena", year = "2012", abstract = "Murashige and Skoog medium (MS) and modified Anderson's Rhododendron medium (mAN) were compared for in vitro shoot multiplication of three highbush blueberries 'Berkeley', 'Bluecrop' and 'Goldtraube'. All media contained 0.5 mg/L zeatin applied either alone or combined with 0.1, 1 and 5 mg/L IBA. In vitro rooting was induced using mAN medium supplemented with 0.8 mg/L IBA and 4 g/L activated charcoal. The results obtained showed that mAN medium is more suitable for in vitro multiplication of the selected highbush blueberry cultivars than MS medium. Low concentration of IBA ( LT = 1 mg/L) added in zeatin-supplemented mAN medium increases shoot multiplication efficiency of highbush blueberries in vitro and can be recommended for large-scale propagation of high quality plants. MS medium induced partial or full necrosis of stems and leaves, which was more pronounced on media containing zeatin combined with increasing concentration of IBA. Rooting capacity of shoots varied widely among the tested blueberry cultivars. The highest rooting and acclimatisation rates were achieved in 'Goldtraube' (82.8 and 91.8% respectively), and the lowest (10 and 66.7% respectively) were in 'Berkeley'.", publisher = "International Society for Horticultural Science", journal = "Acta Horticulturae", title = "Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.)", pages = "272-265", volume = "926", doi = "10.17660/ActaHortic.2012.926.36", url = "conv_1745" }
Ružić, Đ., Vujović, T., Cerović, R., Ostrolucka, M. G.,& Gajdosova, A.. (2012). Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.). in Acta Horticulturae International Society for Horticultural Science., 926, 265-272. https://doi.org/10.17660/ActaHortic.2012.926.36 conv_1745
Ružić Đ, Vujović T, Cerović R, Ostrolucka MG, Gajdosova A. Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.). in Acta Horticulturae. 2012;926:265-272. doi:10.17660/ActaHortic.2012.926.36 conv_1745 .
Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, Ostrolucka, M. G., Gajdosova, Alena, "Micropropagation In Vitro of Highbush Blueberry (Vaccinium corymbosum L.)" in Acta Horticulturae, 926 (2012):265-272, https://doi.org/10.17660/ActaHortic.2012.926.36 ., conv_1745 .