The capacity of regeneration of adventitious shoots from leaf explants was studied in sour cherry 'aanski Rubin' (Prunus cerasus L.) and cherry rootstock Gisela 5 (P. cerasus x P. canescens). Regeneration assay included thirty different combinations of plant growth regulators. 6-benzyladenine (BA) and thidiazuron (TDZ) were applied either individually or each combined with different concentrations of indole-3-butyric acid, alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). 'aanski Rubin' showed higher regeneration capacity in comparison with Gisela 5 regarding the total number of treatments inducing regeneration as well as the highest frequency of regeneration achieved. In both genotypes, 8.9 mu M BA was more effective than both 4.5 and 9.0 mu M TDZ in inducing adventitious regeneration, but only when combined with auxins. The highest frequency of regeneration (20.8 %) in 'aanski Rubin' was achieved on medium supplemented with 8.9 mu M BA combined with 5.4 m...u M NAA, while in Gisela 5 the highest value (8.3 %) was obtained when BA was combined with 4.5 mu M 2,4-D. Flow cytometry combined with 4'-6-diamidino-2-phenylindole staining was employed to estimate DNA ploidy levels and relative nuclear DNA content in adventitious regeneration-derived shoots, in vitro shoots of axillary origin and in vivo control plants from open field. No significant differences in nuclear DNA content were detected among plants of different origin. Chromosome counting in root tip meristems also showed normal tetraploid chromosome number (2n = 4x = 32) in 'aanski Rubin' shoots and normal triploid chromosome number (2n = 3x = 24) in Gisela 5 shoots regenerated in vitro. The results obtained suggest that no major genetic instability occurred during adventitious regeneration under the described experimental conditions.
TY - JOUR
AU - Vujović, Tatjana
AU - Cerović, Radosav
AU - Ružić, Đurđina
PY - 2012
UR - https://refri.institut-cacak.org/handle/123456789/230
AB - The capacity of regeneration of adventitious shoots from leaf explants was studied in sour cherry 'aanski Rubin' (Prunus cerasus L.) and cherry rootstock Gisela 5 (P. cerasus x P. canescens). Regeneration assay included thirty different combinations of plant growth regulators. 6-benzyladenine (BA) and thidiazuron (TDZ) were applied either individually or each combined with different concentrations of indole-3-butyric acid, alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). 'aanski Rubin' showed higher regeneration capacity in comparison with Gisela 5 regarding the total number of treatments inducing regeneration as well as the highest frequency of regeneration achieved. In both genotypes, 8.9 mu M BA was more effective than both 4.5 and 9.0 mu M TDZ in inducing adventitious regeneration, but only when combined with auxins. The highest frequency of regeneration (20.8 %) in 'aanski Rubin' was achieved on medium supplemented with 8.9 mu M BA combined with 5.4 mu M NAA, while in Gisela 5 the highest value (8.3 %) was obtained when BA was combined with 4.5 mu M 2,4-D. Flow cytometry combined with 4'-6-diamidino-2-phenylindole staining was employed to estimate DNA ploidy levels and relative nuclear DNA content in adventitious regeneration-derived shoots, in vitro shoots of axillary origin and in vivo control plants from open field. No significant differences in nuclear DNA content were detected among plants of different origin. Chromosome counting in root tip meristems also showed normal tetraploid chromosome number (2n = 4x = 32) in 'aanski Rubin' shoots and normal triploid chromosome number (2n = 3x = 24) in Gisela 5 shoots regenerated in vitro. The results obtained suggest that no major genetic instability occurred during adventitious regeneration under the described experimental conditions.
PB - Springer, Dordrecht
T2 - Plant Cell Tissue and Organ Culture
T1 - Ploidy level stability of adventitious shoots of sour cherry 'aanski Rubin' and Gisela 5 cherry rootstock
EP - 333
IS - 3
SP - 323
VL - 111
DO - 10.1007/s11240-012-0197-7
UR - conv_1451
ER -
@article{
author = "Vujović, Tatjana and Cerović, Radosav and Ružić, Đurđina",
year = "2012",
abstract = "The capacity of regeneration of adventitious shoots from leaf explants was studied in sour cherry 'aanski Rubin' (Prunus cerasus L.) and cherry rootstock Gisela 5 (P. cerasus x P. canescens). Regeneration assay included thirty different combinations of plant growth regulators. 6-benzyladenine (BA) and thidiazuron (TDZ) were applied either individually or each combined with different concentrations of indole-3-butyric acid, alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). 'aanski Rubin' showed higher regeneration capacity in comparison with Gisela 5 regarding the total number of treatments inducing regeneration as well as the highest frequency of regeneration achieved. In both genotypes, 8.9 mu M BA was more effective than both 4.5 and 9.0 mu M TDZ in inducing adventitious regeneration, but only when combined with auxins. The highest frequency of regeneration (20.8 %) in 'aanski Rubin' was achieved on medium supplemented with 8.9 mu M BA combined with 5.4 mu M NAA, while in Gisela 5 the highest value (8.3 %) was obtained when BA was combined with 4.5 mu M 2,4-D. Flow cytometry combined with 4'-6-diamidino-2-phenylindole staining was employed to estimate DNA ploidy levels and relative nuclear DNA content in adventitious regeneration-derived shoots, in vitro shoots of axillary origin and in vivo control plants from open field. No significant differences in nuclear DNA content were detected among plants of different origin. Chromosome counting in root tip meristems also showed normal tetraploid chromosome number (2n = 4x = 32) in 'aanski Rubin' shoots and normal triploid chromosome number (2n = 3x = 24) in Gisela 5 shoots regenerated in vitro. The results obtained suggest that no major genetic instability occurred during adventitious regeneration under the described experimental conditions.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Ploidy level stability of adventitious shoots of sour cherry 'aanski Rubin' and Gisela 5 cherry rootstock",
pages = "333-323",
number = "3",
volume = "111",
doi = "10.1007/s11240-012-0197-7",
url = "conv_1451"
}
Vujović, T., Cerović, R.,& Ružić, Đ.. (2012). Ploidy level stability of adventitious shoots of sour cherry 'aanski Rubin' and Gisela 5 cherry rootstock. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 111(3), 323-333.
https://doi.org/10.1007/s11240-012-0197-7
conv_1451
Vujović T, Cerović R, Ružić Đ. Ploidy level stability of adventitious shoots of sour cherry 'aanski Rubin' and Gisela 5 cherry rootstock. in Plant Cell Tissue and Organ Culture. 2012;111(3):323-333.
doi:10.1007/s11240-012-0197-7
conv_1451 .
Vujović, Tatjana, Cerović, Radosav, Ružić, Đurđina, "Ploidy level stability of adventitious shoots of sour cherry 'aanski Rubin' and Gisela 5 cherry rootstock" in Plant Cell Tissue and Organ Culture, 111, no. 3 (2012):323-333,
https://doi.org/10.1007/s11240-012-0197-7 .,
conv_1451 .
