In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks
Апстракт
In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found i...n successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.
Кључне речи:
rooting / plum / pear / micropropagation / cherryИзвор:
Horticultural Science, 2012, 39, 3, 101-107Издавач:
- Czech Academy Agricultural Sciences, Prague
Финансирање / пројекти:
- Стварање и проучавање нових генотипова воћака и увођење савремених биотехнологија гајења и прераде воћа (RS-MESTD-MPN2006-2010-20013)
- Стварање и очување генетичког потенцијала континенталних врста воћака (RS-MESTD-Technological Development (TD or TR)-31064)
DOI: 10.17221/208/2011-HORTSCI
ISSN: 0862-867X
WoS: 000307974300001
Scopus: 2-s2.0-84866109812
Институција/група
Institut za voćarstvoTY - JOUR AU - Vujović, Tatjana AU - Ružić, Đurđina AU - Cerović, Radosav PY - 2012 UR - https://refri.institut-cacak.org/handle/123456789/228 AB - In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse. PB - Czech Academy Agricultural Sciences, Prague T2 - Horticultural Science T1 - In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks EP - 107 IS - 3 SP - 101 VL - 39 DO - 10.17221/208/2011-HORTSCI UR - conv_1449 ER -
@article{ author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav", year = "2012", abstract = "In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Horticultural Science", title = "In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks", pages = "107-101", number = "3", volume = "39", doi = "10.17221/208/2011-HORTSCI", url = "conv_1449" }
Vujović, T., Ružić, Đ.,& Cerović, R.. (2012). In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. in Horticultural Science Czech Academy Agricultural Sciences, Prague., 39(3), 101-107. https://doi.org/10.17221/208/2011-HORTSCI conv_1449
Vujović T, Ružić Đ, Cerović R. In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. in Horticultural Science. 2012;39(3):101-107. doi:10.17221/208/2011-HORTSCI conv_1449 .
Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks" in Horticultural Science, 39, no. 3 (2012):101-107, https://doi.org/10.17221/208/2011-HORTSCI ., conv_1449 .