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Raspberry bushy dwarf virus: A grapevine pathogen in Serbia

dc.creatorJevremović, Darko
dc.creatorPaunović, Svetlana A.
dc.date.accessioned2023-04-11T12:26:38Z
dc.date.available2023-04-11T12:26:38Z
dc.date.issued2011
dc.identifier.issn1820-3949
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/212
dc.description.abstractPregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom analizom fragmenta iz 5' regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove loze ovim virusom saopšten je u Sloveniji 2003. godine.sr
dc.description.abstractDuring a field survey in 2005 in vineyards and grapevine nurseries at localities in central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment in 5' part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity with the compared RBDV sequence originating from raspberry. The finding of Raspberry bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine worldwide. The first natural infection of grapevine with this virus was reported in Slovenia in 2003.en
dc.publisherInstitut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
dc.relationProjekat Ministarstva nauke Republike Srbije, br. 20013: Development and study of newly bred fruit genotypes, and introduction of modern biotechnologies of fruit growing and processing
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.sourcePesticidi i fitomedicina
dc.subjectvirus žbunaste kržljavosti malinesr
dc.subjectvinova lozasr
dc.subjectnested-PCRsr
dc.subjectdetekcijasr
dc.subjectDAS-ELISAsr
dc.subjectraspberry bushy dwarf virusen
dc.subjectnested-PCRen
dc.subjectgrapevineen
dc.subjectdetectionen
dc.subjectDAS-ELISAen
dc.titleVirus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbijisr
dc.titleRaspberry bushy dwarf virus: A grapevine pathogen in Serbiaen
dc.typearticle
dc.rights.licenseBY-SA
dc.citation.epage60
dc.citation.issue1
dc.citation.other26(1): 55-60
dc.citation.rankM24
dc.citation.spage55
dc.citation.volume26
dc.identifier.doi10.2298/PIF1101055J
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/61/209.pdf
dc.identifier.rcubconv_1296
dc.type.versionpublishedVersion


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