Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji
Raspberry bushy dwarf virus: A grapevine pathogen in Serbia
Апстракт
Pregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom analizom fragmenta iz 5' regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove loze ovim virusom saopšten je u Sloveniji 2003. godine.
During a field survey in 2005 in vineyards and grapevine nurseries at localities in central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment in 5' part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity with the compared RBDV sequence originating from raspberry. The finding of Raspberry bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine worldwide. The first natural infection of grapevine with this virus was reported in Slovenia in 2003.
Кључне речи:
virus žbunaste kržljavosti maline / vinova loza / nested-PCR / detekcija / DAS-ELISA / raspberry bushy dwarf virus / nested-PCR / grapevine / detection / DAS-ELISAИзвор:
Pesticidi i fitomedicina, 2011, 26, 1, 55-60Издавач:
- Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
Финансирање / пројекти:
- Projekat Ministarstva nauke Republike Srbije, br. 20013: Development and study of newly bred fruit genotypes, and introduction of modern biotechnologies of fruit growing and processing
Институција/група
Institut za voćarstvoTY - JOUR AU - Jevremović, Darko AU - Paunović, Svetlana A. PY - 2011 UR - https://refri.institut-cacak.org/handle/123456789/212 AB - Pregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom analizom fragmenta iz 5' regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove loze ovim virusom saopšten je u Sloveniji 2003. godine. AB - During a field survey in 2005 in vineyards and grapevine nurseries at localities in central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment in 5' part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity with the compared RBDV sequence originating from raspberry. The finding of Raspberry bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine worldwide. The first natural infection of grapevine with this virus was reported in Slovenia in 2003. PB - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd T2 - Pesticidi i fitomedicina T1 - Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji T1 - Raspberry bushy dwarf virus: A grapevine pathogen in Serbia EP - 60 IS - 1 SP - 55 VL - 26 DO - 10.2298/PIF1101055J UR - conv_1296 ER -
@article{ author = "Jevremović, Darko and Paunović, Svetlana A.", year = "2011", abstract = "Pregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom analizom fragmenta iz 5' regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove loze ovim virusom saopšten je u Sloveniji 2003. godine., During a field survey in 2005 in vineyards and grapevine nurseries at localities in central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment in 5' part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity with the compared RBDV sequence originating from raspberry. The finding of Raspberry bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine worldwide. The first natural infection of grapevine with this virus was reported in Slovenia in 2003.", publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd", journal = "Pesticidi i fitomedicina", title = "Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji, Raspberry bushy dwarf virus: A grapevine pathogen in Serbia", pages = "60-55", number = "1", volume = "26", doi = "10.2298/PIF1101055J", url = "conv_1296" }
Jevremović, D.,& Paunović, S. A.. (2011). Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji. in Pesticidi i fitomedicina Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(1), 55-60. https://doi.org/10.2298/PIF1101055J conv_1296
Jevremović D, Paunović SA. Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji. in Pesticidi i fitomedicina. 2011;26(1):55-60. doi:10.2298/PIF1101055J conv_1296 .
Jevremović, Darko, Paunović, Svetlana A., "Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) - patogen vinove loze u Srbiji" in Pesticidi i fitomedicina, 26, no. 1 (2011):55-60, https://doi.org/10.2298/PIF1101055J ., conv_1296 .
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