Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants
Само за регистроване кориснике
2010
Чланак у часопису (Објављена верзија)
![](/themes/MirageReFRI//images/creativecommons/arr.png)
Метаподаци
Приказ свих података о документуАпстракт
Adventitious shoot regeneration from leaves of blackberry cultivar aanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N (6)-benzylaminopurine (BAP) (2.0 mg l(-1)) or thidiazuron (TDZ) (1.0 and 2.0 mg l(-1)) alone, or either of them combined with indol-3-butyric acid (IBA), alpha-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l(-1)). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l(-1), applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses... were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of aanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3',5,5'-tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection.
Кључне речи:
Isoperoxidase / Flow cytometry / Cytokinins / Chromosome number / Auxins / Adventitious organogenesisИзвор:
Plant Growth Regulation, 2010, 61, 3, 265-275Издавач:
- Springer, Dordrecht
DOI: 10.1007/s10725-010-9474-9
ISSN: 0167-6903
WoS: 000278411500006
Scopus: 2-s2.0-77953479780
Институција/група
Institut za voćarstvoTY - JOUR AU - Vujović, Tatjana AU - Ružić, Đurđina AU - Cerović, Radosav AU - Surlan-Momirović, Gordana PY - 2010 UR - https://refri.institut-cacak.org/handle/123456789/180 AB - Adventitious shoot regeneration from leaves of blackberry cultivar aanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N (6)-benzylaminopurine (BAP) (2.0 mg l(-1)) or thidiazuron (TDZ) (1.0 and 2.0 mg l(-1)) alone, or either of them combined with indol-3-butyric acid (IBA), alpha-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l(-1)). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l(-1), applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of aanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3',5,5'-tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection. PB - Springer, Dordrecht T2 - Plant Growth Regulation T1 - Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants EP - 275 IS - 3 SP - 265 VL - 61 DO - 10.1007/s10725-010-9474-9 UR - conv_1386 ER -
@article{ author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav and Surlan-Momirović, Gordana", year = "2010", abstract = "Adventitious shoot regeneration from leaves of blackberry cultivar aanska Bestrna was examined using 30 different combinations of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS) medium containing either N (6)-benzylaminopurine (BAP) (2.0 mg l(-1)) or thidiazuron (TDZ) (1.0 and 2.0 mg l(-1)) alone, or either of them combined with indol-3-butyric acid (IBA), alpha-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l(-1)). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations of plant growth regulators. TDZ at 1.0 mg l(-1), applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in root tip meristems, and flow cytometry analysis indicated that adventitious shoots of aanska Bestrna are tetraploid with 2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated plants). These differences were visible with 3,3',5,5'-tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection.", publisher = "Springer, Dordrecht", journal = "Plant Growth Regulation", title = "Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants", pages = "275-265", number = "3", volume = "61", doi = "10.1007/s10725-010-9474-9", url = "conv_1386" }
Vujović, T., Ružić, Đ., Cerović, R.,& Surlan-Momirović, G.. (2010). Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants. in Plant Growth Regulation Springer, Dordrecht., 61(3), 265-275. https://doi.org/10.1007/s10725-010-9474-9 conv_1386
Vujović T, Ružić Đ, Cerović R, Surlan-Momirović G. Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants. in Plant Growth Regulation. 2010;61(3):265-275. doi:10.1007/s10725-010-9474-9 conv_1386 .
Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, Surlan-Momirović, Gordana, "Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants" in Plant Growth Regulation, 61, no. 3 (2010):265-275, https://doi.org/10.1007/s10725-010-9474-9 ., conv_1386 .