Uticaj temperatura na formiranje kalusa pri različitim varijantama stratifikovanja oraha

Abstract

Ispitivan je uticaj različitih varijanti stratifikovanja na početak formiranja kalusa kod pet sorti i selekcija oraha: Šeinovo, Ovčar, Elit, G-139 i G-286. Temperature stratifikale od 28-29°C tokom 2004. godine inicarale su raniji početak formiranja kalusa u odnosu na temperature od 26-28°C tokom 2003. godine u svim varijantama stratifikovanja. Kalusiranje je prvo nastupilo kod varijante sa parafinisanjem plemke i spojnog mesta, stratifikovane strugotinom do spojnog mesta i prekrivene polietilenskom folijom. Zatim kod varijante sa parafinisanjem plemke i spojnog mesta, stratifikovane strugotinom do vrha plemke. Najkasniji početak kalusiranja nastupio je kod varijante bez parafinisanja kalemova potpuno prekrivene četinarskom strugotinom tj. do vrha plemke. Posmatrano po sortama i selekcijama, kalus se najpre pojavio kod sorte Šeinovo, selekcija Ovčar i G-286, a kasnije kod selekcija G-139 i Elit.

In this paper the effect of different stratification variants on callus formation onset in five walnut cultivars and selections ('Šeinovo', 'Ovčar', 'Elit', G-139 and G-286) was studied. In all variants of stratification, temperatures 28-29°C during 2004 initiated earlier onset of callus formation as compared to those 2003 (26-28°C). Callusing was first observed in the variant that included dipping the scion and union into paraffin with the stratification in the sawdust up to the union and covering with polyethylene foil. Callusing was then observed in the variant that included dipping the scion and union into paraffin, with the stratification in the sawdust up to the scion top. Callusing was observed latest in the variant without paraffin by complete covering with coniferous sawdust. Classified by cultivars and selections, callus formation was first observed in 'Šeinovo' and selections 'Ovčar' and 'G-286', whereas it was only later recorded in selections 'G- 139' and 'Elit'.