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Establishment of aseptic culture in vitro for new vegetative rootstocks for cherry, pear and plum

dc.creatorRužić, Đurđina
dc.creatorCerović, Radosav
dc.creatorVujović, Tatjana
dc.date.accessioned2023-04-11T12:22:21Z
dc.date.available2023-04-11T12:22:21Z
dc.date.issued2010
dc.identifier.issn1820-5054
dc.identifier.urihttps://refri.institut-cacak.org/handle/123456789/174
dc.description.abstractProcedura sterilizacije eksplantata, kao prva faza mikropropagacije je veoma važna za uspešnost organogeneze, posebno ako se početni eksplantati uzimaju iz otvorenog polja, odnosno voćnjaka, ili iz staklara i mrežanika. Standardna procedura površinske sterilizacije, vremenski modifikovana je primenjena na podlogama Gisela 5, Pyrodwarf i Fereley Jaspi. Dve vrste eksplantata su korišćene: pupoljci sa grančica držanih u laboratorijskim uslovima i sa grančica poreklom iz mrežanika. Najveći % indukovanih lisnih rozeta dobijen je uzimanjem eksplantata iz laboratorijskih uslova, posebno kod podloge Gisela 5, 82,7%.sr
dc.description.abstractExplant sterilization procedure, the initial phase of micropropagation, is very important for the successful organogenesis outcome, especially when initial explants are collected from open field (a planting) or glass house/screen house. Standard procedure of surface sterilization, modified in terms of time, has been applied on Gisela, Pyrodward and Fereley Jaspi rootstocks. Two types of explants were used: buds taken from twigs maintained in laboratory, and from those maintained in a screen house. The highest rate (%) of induced leaf rosettes was obtained from laboratory explants of Gisela 5 (82.7%). Besides type and method of taking explants, which are major factors of successful establishment of sterile culture, the method of surface sterilization, modified in terms of time, has also proved a significant segment. In the procedure of establishment of aseptic culture experience plays an important role. It varies among laboratories, as each micropropagation laboratory introduces new modifications. Nevertheless, any innovation represents a very important contribution to the success of this modern method of plant/fruit propagation which can be widely applied, particularly in commercial laboratories.en
dc.publisherNaučno voćarsko društvo Srbije, Čačak
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/20013/RS//
dc.rightsopenAccess
dc.sourceVoćarstvo
dc.subjectvrsta eksplantatasr
dc.subjectprocedura sterilizacijesr
dc.subjectnove podloge voćakasr
dc.subjectindukcija lisne rozetesr
dc.subjecttype of explantsen
dc.subjectsterilization procedureen
dc.subjectnew fruit rootstocksen
dc.subjectinitiation of leaf rosetteen
dc.titleUspostavljanje aseptične kulture in vitro novih vegetativnih podloga za trešnju, krušku i šljivusr
dc.titleEstablishment of aseptic culture in vitro for new vegetative rootstocks for cherry, pear and plumen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage41
dc.citation.issue169-170
dc.citation.other44(169-170): 35-41
dc.citation.rankM51
dc.citation.spage35
dc.citation.volume44
dc.identifier.fulltexthttp://refri.institut-cacak.org/bitstream/id/36/171.pdf
dc.identifier.rcubconv_1316
dc.type.versionpublishedVersion


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