The Polymorphism of the Genes Involved in Ethylene Biosynthesis and Perception in Apple

Abstract

Apple (Malus x domestica Borkh.) is a climacteric fruit whose ripening is associated with a burst of autocatalytic production of hormone ethylene. Although the ethylene biosynthetic pathway is well characterised, understanding of the molecular mechanisms underlying ethylene perception and signal transduction pathway is limited. Little information is available about the functional relevance of allelic polymorphism of the genes involved in ethylene biosynthesis and perception. We have studied polymorphism in the ACS1, ACO1 and ETR1 genes, encoding for ACC synthase, ACC oxidase and ethylene receptor respectively, in apple cultivars characterised by different ripening time and storage capability. Two alleles of ACS1 (ACS1-1 and ACS1-2), five alleles of ACO1 (a, b, c, d and n) and five alleles of ETR1 gene (a, b, c, d and e) were detected. The position of all three genes on the apple genetic map was determined.