In this study a spot nested RT-PCR assay was developed for the detection of Apple stem pitting virus (ASPV). A one step RT-PCR for the generic detection of foveaviruses using degenerate primers that target a conserved region of the RNA-dependent RNA polymerase (RdRp) gene was followed by a nested PCR that amplifies a 312 bp ASPV specific product. The method is rapid, simple and displays high sensitivity and broad detection range, overcoming the virus molecular variability. The optimum sampling conditions for reliable virus detection were also investigated. ASPV was detected throughout the year in different plant tissues of affected trees, thus the method could be used for routine screening and in certification schemes of pome fruits. ASPV was detected in quince orchards in Greece in all trees that were tested, showing a fruit deformation disorder. Sequencing and phylogenetic analysis of amplicons generated by RT-PCR from plant tissue affected with the deformation disease indicated that... the agent responsible was a variant of ASPV.
TY - JOUR
AU - Mathioudakis, M. M.
AU - Maliogka, V. I.
AU - Dovas, C. I.
AU - Paunović, Svetlana A.
AU - Katis, N I.
PY - 2009
UR - https://refri.institut-cacak.org/handle/123456789/148
AB - In this study a spot nested RT-PCR assay was developed for the detection of Apple stem pitting virus (ASPV). A one step RT-PCR for the generic detection of foveaviruses using degenerate primers that target a conserved region of the RNA-dependent RNA polymerase (RdRp) gene was followed by a nested PCR that amplifies a 312 bp ASPV specific product. The method is rapid, simple and displays high sensitivity and broad detection range, overcoming the virus molecular variability. The optimum sampling conditions for reliable virus detection were also investigated. ASPV was detected throughout the year in different plant tissues of affected trees, thus the method could be used for routine screening and in certification schemes of pome fruits. ASPV was detected in quince orchards in Greece in all trees that were tested, showing a fruit deformation disorder. Sequencing and phylogenetic analysis of amplicons generated by RT-PCR from plant tissue affected with the deformation disease indicated that the agent responsible was a variant of ASPV.
PB - Wiley, Hoboken
T2 - Plant Pathology
T1 - Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease
EP - 236
IS - 2
SP - 228
VL - 58
DO - 10.1111/j.1365-3059.2008.01952.x
UR - conv_1375
ER -
@article{
author = "Mathioudakis, M. M. and Maliogka, V. I. and Dovas, C. I. and Paunović, Svetlana A. and Katis, N I.",
year = "2009",
abstract = "In this study a spot nested RT-PCR assay was developed for the detection of Apple stem pitting virus (ASPV). A one step RT-PCR for the generic detection of foveaviruses using degenerate primers that target a conserved region of the RNA-dependent RNA polymerase (RdRp) gene was followed by a nested PCR that amplifies a 312 bp ASPV specific product. The method is rapid, simple and displays high sensitivity and broad detection range, overcoming the virus molecular variability. The optimum sampling conditions for reliable virus detection were also investigated. ASPV was detected throughout the year in different plant tissues of affected trees, thus the method could be used for routine screening and in certification schemes of pome fruits. ASPV was detected in quince orchards in Greece in all trees that were tested, showing a fruit deformation disorder. Sequencing and phylogenetic analysis of amplicons generated by RT-PCR from plant tissue affected with the deformation disease indicated that the agent responsible was a variant of ASPV.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease",
pages = "236-228",
number = "2",
volume = "58",
doi = "10.1111/j.1365-3059.2008.01952.x",
url = "conv_1375"
}
Mathioudakis, M. M., Maliogka, V. I., Dovas, C. I., Paunović, S. A.,& Katis, N. I.. (2009). Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease. in Plant Pathology
Wiley, Hoboken., 58(2), 228-236.
https://doi.org/10.1111/j.1365-3059.2008.01952.x
conv_1375
Mathioudakis MM, Maliogka VI, Dovas CI, Paunović SA, Katis NI. Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease. in Plant Pathology. 2009;58(2):228-236.
doi:10.1111/j.1365-3059.2008.01952.x
conv_1375 .
Mathioudakis, M. M., Maliogka, V. I., Dovas, C. I., Paunović, Svetlana A., Katis, N I., "Reliable RT-PCR detection of Apple stem pitting virus in pome fruits and its association with quince fruit deformation disease" in Plant Pathology, 58, no. 2 (2009):228-236,
https://doi.org/10.1111/j.1365-3059.2008.01952.x .,
conv_1375 .
