TY  - CONF
AU  - Vasić, Tanja
AU  - Živković, Sanja
AU  - Katanić, Vera
AU  - Vasilijević, Bojana
AU  - Jevremović, Darko
AU  - Marković, Jordan
PY  - 2024-03
UR  - https://refri.institut-cacak.org/handle/123456789/869
AB  - What often happens that weeds are not controlled after harvesting
the cultivated crop. Thus, they continue to grow unhindered on agricultural
land, becoming dangerous hosts for a large number of plant diseases and
sources of constant infection. So far, there have been no systematic studies of
the mycopopulation of weed seeds in Serbia. In this paper, we present the
results of preliminary research. Weed seeds samples were collected during the
summer and autumn vegetation of 2023. Isolation was performed using
standard phytopathological methods. In these studies of the mycopopulation of
weed seeds, a total of 500 seeds originating from ten weed species were
examined. Seven genera of fungi were determined in this way: Penicillium,
Fusarium, Alternaria, Cladosporium, Epicoccum, Rhizopus and Mucor
PB  - Čačak : University of Kragujevac, Serbia Faculty of Agronomy in Cacak
C3  - Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)
T1  - Mycopopulation on seed of weed plants in organic vegetable production crop
EP  - 322
SP  - 317
DO  - 10.46793/SBT29.41TV
ER  - 

@conference{
author = "Vasić, Tanja and Živković, Sanja and Katanić, Vera and Vasilijević, Bojana and Jevremović, Darko and Marković, Jordan",
year = "2024-03",
abstract = "What often happens that weeds are not controlled after harvestingthe cultivated crop. Thus, they continue to grow unhindered on agriculturalland, becoming dangerous hosts for a large number of plant diseases andsources of constant infection. So far, there have been no systematic studies ofthe mycopopulation of weed seeds in Serbia. In this paper, we present theresults of preliminary research. Weed seeds samples were collected during thesummer and autumn vegetation of 2023. Isolation was performed usingstandard phytopathological methods. In these studies of the mycopopulation ofweed seeds, a total of 500 seeds originating from ten weed species wereexamined. Seven genera of fungi were determined in this way: Penicillium,Fusarium, Alternaria, Cladosporium, Epicoccum, Rhizopus and Mucor",
publisher = "Čačak : University of Kragujevac, Serbia Faculty of Agronomy in Cacak",
journal = "Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)",
title = "Mycopopulation on seed of weed plants in organic vegetable production crop",
pages = "322-317",
doi = "10.46793/SBT29.41TV"
}

Vasić, T., Živković, S., Katanić, V., Vasilijević, B., Jevremović, D.,& Marković, J.. (2024-03). Mycopopulation on seed of weed plants in organic vegetable production crop. in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)
Čačak : University of Kragujevac, Serbia Faculty of Agronomy in Cacak., 317-322.
https://doi.org/10.46793/SBT29.41TV

Vasić T, Živković S, Katanić V, Vasilijević B, Jevremović D, Marković J. Mycopopulation on seed of weed plants in organic vegetable production crop. in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia). 2024;:317-322.
doi:10.46793/SBT29.41TV .

Vasić, Tanja, Živković, Sanja, Katanić, Vera, Vasilijević, Bojana, Jevremović, Darko, Marković, Jordan, "Mycopopulation on seed of weed plants in organic vegetable production crop" in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia) (2024-03):317-322,
https://doi.org/10.46793/SBT29.41TV . .

TY  - CONF
AU  - Vasilijević, Bojana
AU  - Vujović, Tatjana
AU  - Anđelić, Tatjana
AU  - Katanić, Vera
AU  - Jevremović, Darko
PY  - 2024
UR  - https://refri.institut-cacak.org/handle/123456789/874
AB  - The importance of conserving plant species, particularly those crucial for agriculture, has surged
to ensure sustainable utilization of biological resources and prevent further loss of plant diversity. Cryopreservation, the most advanced method for plant conservation, offers promise in this
regard. It can be employed for conservation per
se, or as a potential tool (cryotherapy) for pathogens elimination from infected plants, particularly viruses that threaten agricultural productivity. In the recent study, two vitrification-based
cryopreservation methods, D and V cryo-plate,
were evaluated for their efficacy in eradicating
plum pox virus (PPV) from autochthonous plum
cultivars ‘Crvena Ranka’ and ‘Belošljiva’, widely
present in the Balkan region. A total of 111 pool
samples of in vitro shoots of plums ‘Belošljiva’
and ‘Crvena Ranka’ were tested (65 and 46 samples, respectively). Each sample consisted of an
average of 10 plants, in total about 1100 plants.
Health status of in vitro shoots originated from
both control (non-frozen explants) and cryopreserved explants of infected plums were continuously tested during multiplication for the PPV
presence with conventional reverse transcription
polymerase chain reaction (RT-PCR). To evaluate
these results and obtain the highest efficiency of
detection, all samples were further tested using
Real-time PCR (qPCR). Using RT-PCR, the PPV was
detected in 67 out of 111 tested samples: in 39
samples of plum ‘Belošljiva’ and 28 of plum ‘Crvena Ranka’. Using a qPCR assay, PPV was confirmed
in 82 tested samples. Effective PPV elimination
from ‘Crvena Ranka’ was achieved through three
cryo-treatments, while neither method (D and
V-cryoplate) nor the applied treatments led to
PPV elimination in ‘Belošljiva’. The qPCR assay
demonstrated higher sensitivity compared to
conventional RT-PCR, highlighting its potential
utility in such assessments. Our findings supported the exceptional specificity and sensitivity of
the qPCR technique in detecting PPV in in vitro
shoots of the plums ‘Belošljiva’ and ‘Crvena Ranka’ post-cryotherapy.
PB  - Belgrade : Serbian Society for Microbiology
C3  - Book of Abstracts : XIII Congress of microbiologists of Serbia  "From biotechnology to human and planetary health", 4th-6th April, 2024, Belgrade, Serbia
T1  - Cryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačak
EP  - 72
SP  - 72
ER  - 

@conference{
author = "Vasilijević, Bojana and Vujović, Tatjana and Anđelić, Tatjana and Katanić, Vera and Jevremović, Darko",
year = "2024",
abstract = "The importance of conserving plant species, particularly those crucial for agriculture, has surgedto ensure sustainable utilization of biological resources and prevent further loss of plant diversity. Cryopreservation, the most advanced method for plant conservation, offers promise in thisregard. It can be employed for conservation perse, or as a potential tool (cryotherapy) for pathogens elimination from infected plants, particularly viruses that threaten agricultural productivity. In the recent study, two vitrification-basedcryopreservation methods, D and V cryo-plate,were evaluated for their efficacy in eradicatingplum pox virus (PPV) from autochthonous plumcultivars ‘Crvena Ranka’ and ‘Belošljiva’, widelypresent in the Balkan region. A total of 111 poolsamples of in vitro shoots of plums ‘Belošljiva’and ‘Crvena Ranka’ were tested (65 and 46 samples, respectively). Each sample consisted of anaverage of 10 plants, in total about 1100 plants.Health status of in vitro shoots originated fromboth control (non-frozen explants) and cryopreserved explants of infected plums were continuously tested during multiplication for the PPVpresence with conventional reverse transcriptionpolymerase chain reaction (RT-PCR). To evaluatethese results and obtain the highest efficiency ofdetection, all samples were further tested usingReal-time PCR (qPCR). Using RT-PCR, the PPV wasdetected in 67 out of 111 tested samples: in 39samples of plum ‘Belošljiva’ and 28 of plum ‘Crvena Ranka’. Using a qPCR assay, PPV was confirmedin 82 tested samples. Effective PPV eliminationfrom ‘Crvena Ranka’ was achieved through threecryo-treatments, while neither method (D andV-cryoplate) nor the applied treatments led toPPV elimination in ‘Belošljiva’. The qPCR assaydemonstrated higher sensitivity compared toconventional RT-PCR, highlighting its potentialutility in such assessments. Our findings supported the exceptional specificity and sensitivity ofthe qPCR technique in detecting PPV in in vitroshoots of the plums ‘Belošljiva’ and ‘Crvena Ranka’ post-cryotherapy.",
publisher = "Belgrade : Serbian Society for Microbiology",
journal = "Book of Abstracts : XIII Congress of microbiologists of Serbia  'From biotechnology to human and planetary health', 4th-6th April, 2024, Belgrade, Serbia",
title = "Cryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačak",
pages = "72-72"
}

Vasilijević, B., Vujović, T., Anđelić, T., Katanić, V.,& Jevremović, D.. (2024). Cryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačak. in Book of Abstracts : XIII Congress of microbiologists of Serbia  "From biotechnology to human and planetary health", 4th-6th April, 2024, Belgrade, Serbia
Belgrade : Serbian Society for Microbiology., 72-72.

Vasilijević B, Vujović T, Anđelić T, Katanić V, Jevremović D. Cryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačak. in Book of Abstracts : XIII Congress of microbiologists of Serbia  "From biotechnology to human and planetary health", 4th-6th April, 2024, Belgrade, Serbia. 2024;:72-72..

Vasilijević, Bojana, Vujović, Tatjana, Anđelić, Tatjana, Katanić, Vera, Jevremović, Darko, "Cryopreservation-based strategy for PPV eradication in autochthonous plums: insights from Fruit Research Institute Čačak" in Book of Abstracts : XIII Congress of microbiologists of Serbia  "From biotechnology to human and planetary health", 4th-6th April, 2024, Belgrade, Serbia (2024):72-72.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Popović, Branko
PY  - 2024
UR  - https://refri.institut-cacak.org/handle/123456789/873
AB  - Plum pox virus (PPV), a potyvirus from the family Potyviridae, is the most detrimental virus of stone fruit species, causing Sharka disease. PPV can significantly decrease fruit quality and yield in sensitive cultivars. It was reported for the first time in Serbia on plums in 1935. Out of the ten identified, only three major PPV strains are present in Serbia: PPV-M, PPV-D, and PPV-Rec. To explore the occurrence and presence of PPV strains in apricot in Serbia, field surveys were carried out from 2008 to 2022. Visual inspections confirmed that a very low percentage of the trees exhibit the leaf symptoms of Sharka. A total of 282 fresh leaf samples were collected from different locations across the country. Leaf samples were analyzed by immunocapture - reverse transcription - polymerase chain reaction (IC-RT-PCR) using strain-specific primers targeting two genomic regions (Nter CP and CI). PPV was confirmed in 86 samples (30.5%). PPV-Rec was the most prevalent strain (52 samples), followed by PPV-M (21 samples) and PPV-D (19 samples). Mixed infection (PPV-Rec + PPV-D) was confirmed in six samples. Although widely present in plums, PPV was less frequently found in apricots in Serbia. The presented results confirmed the wide distribution and dominant presence of PPV-Rec isolates in apricots in Serbia. The genetic diversity of the PPV isolates was evaluated by sequencing a PCR fragment located in the CterNib-Nter CP and CI coding regions for selected isolates.
PB  - Montfavet : INRAE - GAFL, France
C3  - Book of Аbstracts : I International Symposium on Apricot and Plum, April 22-26, Avignon, France
T1  - Molecular characterization of plum pox virus isolates in apricot in Serbia
EP  - 33
SP  - 32
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Popović, Branko",
year = "2024",
abstract = "Plum pox virus (PPV), a potyvirus from the family Potyviridae, is the most detrimental virus of stone fruit species, causing Sharka disease. PPV can significantly decrease fruit quality and yield in sensitive cultivars. It was reported for the first time in Serbia on plums in 1935. Out of the ten identified, only three major PPV strains are present in Serbia: PPV-M, PPV-D, and PPV-Rec. To explore the occurrence and presence of PPV strains in apricot in Serbia, field surveys were carried out from 2008 to 2022. Visual inspections confirmed that a very low percentage of the trees exhibit the leaf symptoms of Sharka. A total of 282 fresh leaf samples were collected from different locations across the country. Leaf samples were analyzed by immunocapture - reverse transcription - polymerase chain reaction (IC-RT-PCR) using strain-specific primers targeting two genomic regions (Nter CP and CI). PPV was confirmed in 86 samples (30.5%). PPV-Rec was the most prevalent strain (52 samples), followed by PPV-M (21 samples) and PPV-D (19 samples). Mixed infection (PPV-Rec + PPV-D) was confirmed in six samples. Although widely present in plums, PPV was less frequently found in apricots in Serbia. The presented results confirmed the wide distribution and dominant presence of PPV-Rec isolates in apricots in Serbia. The genetic diversity of the PPV isolates was evaluated by sequencing a PCR fragment located in the CterNib-Nter CP and CI coding regions for selected isolates.",
publisher = "Montfavet : INRAE - GAFL, France",
journal = "Book of Аbstracts : I International Symposium on Apricot and Plum, April 22-26, Avignon, France",
title = "Molecular characterization of plum pox virus isolates in apricot in Serbia",
pages = "33-32"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Popović, B.. (2024). Molecular characterization of plum pox virus isolates in apricot in Serbia. in Book of Аbstracts : I International Symposium on Apricot and Plum, April 22-26, Avignon, France
Montfavet : INRAE - GAFL, France., 32-33.

Jevremović D, Vasilijević B, Katanić V, Popović B. Molecular characterization of plum pox virus isolates in apricot in Serbia. in Book of Аbstracts : I International Symposium on Apricot and Plum, April 22-26, Avignon, France. 2024;:32-33..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Popović, Branko, "Molecular characterization of plum pox virus isolates in apricot in Serbia" in Book of Аbstracts : I International Symposium on Apricot and Plum, April 22-26, Avignon, France (2024):32-33.

TY  - CONF
AU  - Jevremović, Darko
AU  - Leposavić, Aleksandar
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
PY  - 2024
UR  - https://refri.institut-cacak.org/handle/123456789/872
AB  - Do danas je u svetskoj literaturi opisano preko 30 virusnih bolesti maline. Simptomi ovih obolјenja se ispolјavaju na različitim bilјnim organima. Najuočlјiviji su na listovima i plodovima. Na listovima se javlјa mozaik, crtičavost, šarenilo, hloroza i žutilo liske, nekroza lisnih nerava, uvijanje liske, skraćenje lisnih nerava i druge deformacije. Plodovi zaraženih bilјaka osetlјivih sorti su sitniji, sa manjim brojem koštunica i deformisani što smanjuje njihovu komercijalnu vrednost.
Ekonomski najznačajnije virusi maline su: virus nekroze crne maline (black raspberry necrosis virus, BRNV), virus žute mrežavosti rubusa (Rubus yellow net virus, RYNV), virus šarenila lista maline (raspberry leaf mottle virus, RLMV), virus žbunaste kržlјavosti maline (raspberry bushy dwarf virus, RBDV) i virus mrlјavosti lista maline (raspberry leaf blotch virus, RLBV).
Od navedenih virusa, u Srbiji je najrasprostranjeniji virus mrlјavosti lista maline koji je utvrđen u svim lokalitetima gde se gaji malina. Simptomi koje izaziva RLBV (šarenilo lišća, mrlјavost i pegavost) su identični sa oštećenjima koje prouzrokuje eriofidna grinja lista maline (Phyllocoptes gracilis). Kod sorte Vilamet u uslovima jake zaraze može dovesti do smanjenja mase ploda do 30%. Po značaju, izdvaja se i virus žbunaste kržlјavosti maline koji kod osetlјivih sorti (Miker) uslovlјava pojavu rehulјavih plodova. Virus nekroze crne maline ne izaziva vidlјive simptome kod komercijalno gajenih sorti crvene maline, a kod nas je dominantno potvrđen kod sorte Fertodi zamatos. Virus žute mrežavosti rubusa i virus šarenila lista maline su prisutni u velikom broju malinjaka, ali u pojedinačnim infekcijama ne izazivaju vidlјive simptome na sortama maline koje se gaje kod nas. Mešane infekcije, sa dva ili više virusa, su česte u malinjacima i kod tolerantnih sorti mogu izazvati simptome na listovima i plodovima i dovesti do smanjenja prinosa. Latentni virus maline (raspberry latent virus, RpLV) je osim u SAD, potvrđen samo u Srbiji. RpLV u mešanim infekcijama sa RBDV i RLMV može izazvati pojavu rehulјavih plodova. Rehulјavost plodova je sve češća pojava u malinjacima u našoj zemlјi, a uzroci su genetske prirode i/ili virusne infekcije. U toku su ispitivanja primarnog uzroka ove pojave.  
Virusne bolesti se ne mogu lečiti i osnovne mere kontrole su: korišćenje zdravog sadnog materijala, prostorna izolacija zasada, izbor otpornih i tolerantnih sorti, krčenje i uništavanje zaraženih bilјaka i kontrola vektora.
PB  - Beograd : Institut za primenu nauke u poljoprivredi
C3  - Zbornik apstrakata: III stručni skup "Savremene tehnologije i tendencije razvoja poljoprivrede", april 2024. godine, Čačak
T1  - Najznačajnije virusne bolesti maline
EP  - 2
SP  - 1
ER  - 

@conference{
author = "Jevremović, Darko and Leposavić, Aleksandar and Vasilijević, Bojana and Katanić, Vera",
year = "2024",
abstract = "Do danas je u svetskoj literaturi opisano preko 30 virusnih bolesti maline. Simptomi ovih obolјenja se ispolјavaju na različitim bilјnim organima. Najuočlјiviji su na listovima i plodovima. Na listovima se javlјa mozaik, crtičavost, šarenilo, hloroza i žutilo liske, nekroza lisnih nerava, uvijanje liske, skraćenje lisnih nerava i druge deformacije. Plodovi zaraženih bilјaka osetlјivih sorti su sitniji, sa manjim brojem koštunica i deformisani što smanjuje njihovu komercijalnu vrednost.Ekonomski najznačajnije virusi maline su: virus nekroze crne maline (black raspberry necrosis virus, BRNV), virus žute mrežavosti rubusa (Rubus yellow net virus, RYNV), virus šarenila lista maline (raspberry leaf mottle virus, RLMV), virus žbunaste kržlјavosti maline (raspberry bushy dwarf virus, RBDV) i virus mrlјavosti lista maline (raspberry leaf blotch virus, RLBV).Od navedenih virusa, u Srbiji je najrasprostranjeniji virus mrlјavosti lista maline koji je utvrđen u svim lokalitetima gde se gaji malina. Simptomi koje izaziva RLBV (šarenilo lišća, mrlјavost i pegavost) su identični sa oštećenjima koje prouzrokuje eriofidna grinja lista maline (Phyllocoptes gracilis). Kod sorte Vilamet u uslovima jake zaraze može dovesti do smanjenja mase ploda do 30%. Po značaju, izdvaja se i virus žbunaste kržlјavosti maline koji kod osetlјivih sorti (Miker) uslovlјava pojavu rehulјavih plodova. Virus nekroze crne maline ne izaziva vidlјive simptome kod komercijalno gajenih sorti crvene maline, a kod nas je dominantno potvrđen kod sorte Fertodi zamatos. Virus žute mrežavosti rubusa i virus šarenila lista maline su prisutni u velikom broju malinjaka, ali u pojedinačnim infekcijama ne izazivaju vidlјive simptome na sortama maline koje se gaje kod nas. Mešane infekcije, sa dva ili više virusa, su česte u malinjacima i kod tolerantnih sorti mogu izazvati simptome na listovima i plodovima i dovesti do smanjenja prinosa. Latentni virus maline (raspberry latent virus, RpLV) je osim u SAD, potvrđen samo u Srbiji. RpLV u mešanim infekcijama sa RBDV i RLMV može izazvati pojavu rehulјavih plodova. Rehulјavost plodova je sve češća pojava u malinjacima u našoj zemlјi, a uzroci su genetske prirode i/ili virusne infekcije. U toku su ispitivanja primarnog uzroka ove pojave.  Virusne bolesti se ne mogu lečiti i osnovne mere kontrole su: korišćenje zdravog sadnog materijala, prostorna izolacija zasada, izbor otpornih i tolerantnih sorti, krčenje i uništavanje zaraženih bilјaka i kontrola vektora.",
publisher = "Beograd : Institut za primenu nauke u poljoprivredi",
journal = "Zbornik apstrakata: III stručni skup 'Savremene tehnologije i tendencije razvoja poljoprivrede', april 2024. godine, Čačak",
title = "Najznačajnije virusne bolesti maline",
pages = "2-1"
}

Jevremović, D., Leposavić, A., Vasilijević, B.,& Katanić, V.. (2024). Najznačajnije virusne bolesti maline. in Zbornik apstrakata: III stručni skup "Savremene tehnologije i tendencije razvoja poljoprivrede", april 2024. godine, Čačak
Beograd : Institut za primenu nauke u poljoprivredi., 1-2.

Jevremović D, Leposavić A, Vasilijević B, Katanić V. Najznačajnije virusne bolesti maline. in Zbornik apstrakata: III stručni skup "Savremene tehnologije i tendencije razvoja poljoprivrede", april 2024. godine, Čačak. 2024;:1-2..

Jevremović, Darko, Leposavić, Aleksandar, Vasilijević, Bojana, Katanić, Vera, "Najznačajnije virusne bolesti maline" in Zbornik apstrakata: III stručni skup "Savremene tehnologije i tendencije razvoja poljoprivrede", april 2024. godine, Čačak (2024):1-2.

TY  - CONF
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Živković, Sanja
AU  - Vasić, Tanja
AU  - Kovačević, Stefan
AU  - Jevremović, Darko
PY  - 2024
UR  - https://refri.institut-cacak.org/handle/123456789/870
AB  - Grapevine, a significant fruit crop globally, is a host of various
viruses that negatively affect yield, plant vigor, and fruit quality. Multiplex
polymerase chain reaction (mPCR) offers the ability to detect numerous viruses
simultaneously. Our study aimed to evaluate the effectiveness of mRT-PCR for
the detection of nine grapevine viruses in Serbia, including: grapevine fanleaf
virus, grapevine leafroll-associated viruses 1, 2, and 3, grapevine rupestris stem
pitting associated virus, grapevine virus A, grapevine virus B, grapevine fleck
virus, and arabis mosaic virus. This study confirms mRT-PCR as an efficient
method for simultaneous detection of multiple grapevine viruses
PB  - University of Kragujevac, Serbia Faculty of Agronomy in Cacak
PB  - Čačak : University of Kragujevac, Faculty of Agronomy in Čačak
C3  - Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)
T1  - Application of multiplex RT-PCR for grapevine viruses detection
EP  - 146
SP  - 137
DO  - 10.46793/SBT29.18BV
ER  - 

@conference{
author = "Vasilijević, Bojana and Katanić, Vera and Živković, Sanja and Vasić, Tanja and Kovačević, Stefan and Jevremović, Darko",
year = "2024",
abstract = "Grapevine, a significant fruit crop globally, is a host of variousviruses that negatively affect yield, plant vigor, and fruit quality. Multiplexpolymerase chain reaction (mPCR) offers the ability to detect numerous virusessimultaneously. Our study aimed to evaluate the effectiveness of mRT-PCR forthe detection of nine grapevine viruses in Serbia, including: grapevine fanleafvirus, grapevine leafroll-associated viruses 1, 2, and 3, grapevine rupestris stempitting associated virus, grapevine virus A, grapevine virus B, grapevine fleckvirus, and arabis mosaic virus. This study confirms mRT-PCR as an efficientmethod for simultaneous detection of multiple grapevine viruses",
publisher = "University of Kragujevac, Serbia Faculty of Agronomy in Cacak, Čačak : University of Kragujevac, Faculty of Agronomy in Čačak",
journal = "Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)",
title = "Application of multiplex RT-PCR for grapevine viruses detection",
pages = "146-137",
doi = "10.46793/SBT29.18BV"
}

Vasilijević, B., Katanić, V., Živković, S., Vasić, T., Kovačević, S.,& Jevremović, D.. (2024). Application of multiplex RT-PCR for grapevine viruses detection. in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia)
University of Kragujevac, Serbia Faculty of Agronomy in Cacak., 137-146.
https://doi.org/10.46793/SBT29.18BV

Vasilijević B, Katanić V, Živković S, Vasić T, Kovačević S, Jevremović D. Application of multiplex RT-PCR for grapevine viruses detection. in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia). 2024;:137-146.
doi:10.46793/SBT29.18BV .

Vasilijević, Bojana, Katanić, Vera, Živković, Sanja, Vasić, Tanja, Kovačević, Stefan, Jevremović, Darko, "Application of multiplex RT-PCR for grapevine viruses detection" in Proceedings : 2nd International Symposium on Biotechnology, 14–15 March 2024, Čačak (Serbia) (2024):137-146,
https://doi.org/10.46793/SBT29.18BV . .

TY  - JOUR
AU  - Miletić, Nemanja
AU  - Mitić, Milan
AU  - Popović, Branko
AU  - Petković, Marko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Jevremović, Darko
PY  - 2024
UR  - https://refri.institut-cacak.org/handle/123456789/859
AB  - The aim of this study was to determine the changes in chemical composition of fresh red raspberry ‘Willamette’ fruits caused by the presence of raspberry leaf blotch emaravirus (RLBV). In three experimental orchards of ‘Willamette’ raspberry, fruits were harvested from RLBV-free and RLBV-infected plants in 2019 and 2020. Fruits were collected at appropriate maturity stages and further analyzed in terms of total phenolics, total anthocyanins, and selected individual phenolics. In all three experimental orchards, the phenolic profiles of the infected and uninfected fruit samples were considerably different during both studied years. Nonetheless, the intensity of the modifications varied greatly depending on the location and harvest year. Statistical analysis revealed that the influence of RLBV infection on the studied features was undeniable, although the influences of weather conditions and soil composition outweighed the influence of RLBV. Taking into consideration all the experimental and statistical data, it can be concluded that RLBV had an impact on the phenolic profile of raspberry ‘Willamette’ fruits, while sensitivity to environmental conditions and soil composition is emphasized.
PB  - MDPI : Basel, Switzerland
T2  - Horticulturae
T1  - Chemical composition of healthy and raspberry leaf blotch emaravirus-infected red raspberry 'Willamette' fruits
IS  - 2
SP  - 187
VL  - 10
DO  - 10.3390/horticulturae10020187
ER  - 

@article{
author = "Miletić, Nemanja and Mitić, Milan and Popović, Branko and Petković, Marko and Vasilijević, Bojana and Katanić, Vera and Jevremović, Darko",
year = "2024",
abstract = "The aim of this study was to determine the changes in chemical composition of fresh red raspberry ‘Willamette’ fruits caused by the presence of raspberry leaf blotch emaravirus (RLBV). In three experimental orchards of ‘Willamette’ raspberry, fruits were harvested from RLBV-free and RLBV-infected plants in 2019 and 2020. Fruits were collected at appropriate maturity stages and further analyzed in terms of total phenolics, total anthocyanins, and selected individual phenolics. In all three experimental orchards, the phenolic profiles of the infected and uninfected fruit samples were considerably different during both studied years. Nonetheless, the intensity of the modifications varied greatly depending on the location and harvest year. Statistical analysis revealed that the influence of RLBV infection on the studied features was undeniable, although the influences of weather conditions and soil composition outweighed the influence of RLBV. Taking into consideration all the experimental and statistical data, it can be concluded that RLBV had an impact on the phenolic profile of raspberry ‘Willamette’ fruits, while sensitivity to environmental conditions and soil composition is emphasized.",
publisher = "MDPI : Basel, Switzerland",
journal = "Horticulturae",
title = "Chemical composition of healthy and raspberry leaf blotch emaravirus-infected red raspberry 'Willamette' fruits",
number = "2",
pages = "187",
volume = "10",
doi = "10.3390/horticulturae10020187"
}

Miletić, N., Mitić, M., Popović, B., Petković, M., Vasilijević, B., Katanić, V.,& Jevremović, D.. (2024). Chemical composition of healthy and raspberry leaf blotch emaravirus-infected red raspberry 'Willamette' fruits. in Horticulturae
MDPI : Basel, Switzerland., 10(2), 187.
https://doi.org/10.3390/horticulturae10020187

Miletić N, Mitić M, Popović B, Petković M, Vasilijević B, Katanić V, Jevremović D. Chemical composition of healthy and raspberry leaf blotch emaravirus-infected red raspberry 'Willamette' fruits. in Horticulturae. 2024;10(2):187.
doi:10.3390/horticulturae10020187 .

Miletić, Nemanja, Mitić, Milan, Popović, Branko, Petković, Marko, Vasilijević, Bojana, Katanić, Vera, Jevremović, Darko, "Chemical composition of healthy and raspberry leaf blotch emaravirus-infected red raspberry 'Willamette' fruits" in Horticulturae, 10, no. 2 (2024):187,
https://doi.org/10.3390/horticulturae10020187 . .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/868
AB  - Kruška je jedna od najrasprostranjenijih vrsta voćaka u svetu. U odnosu
na koštičave i jagodaste vrste voćaka, krušku i ostale jabučaste vrste, zaražava manji broj virusa i viroida. Virusi kod većine komercijalnih sorti kruške ne izazivaju simptome na lišću, plodovima i drugim biljnim organima. Kod osetljivih sorti, simptomi se javljaju samo na listovima i plodovima. Široko rasprostanjeni i ekonomski značajni virusi kruške su: virus jamičavosti stabla jabuke (apple stem pitting virus, ASPV), virus brazdavosti stabla jabuke (apple stem grooving virus, ASGV), virus hlorotične lisne pegavosti jabuke (apple chlorotic leaf spot virus, ACLSV) i virus mozaika jabuke (apple mosaic virus, ApMV). Od viroida, po štetnosti se izdvajaju viroid mehuravih rak rana kruške (pear blister canker viroid, PBCVd) i viroid plutavosti pokožice ploda jabuke (apple scar skin viroid, ASSVd).
AB  - One of the most common fruit species in the world is the pears. Compared
to stone and small fruit trees, pears and other pome fruits are infected by a lesser number of viruses and viroids. The majority of commercial pear cultivars do not exhibit symptoms on their leaves, fruits, or other plant parts due to viruses. Only foliage and fruits show symptoms in susceptible cultivars. Apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and apple mosaic virus (ApMV) are widespread and economically significant pear viruses. Pear blister canker viroid (PBCVd) and apple scar skin viroid (ASSVd) are the most dangerous viroids infecting pears.
PB  - Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine
T2  - Biljni Lekar
T1  - Virusi i viroidi kruške
T1  - Viruses and viroids infecting pears
EP  - 774
IS  - 6
SP  - 764
VL  - 51
DO  - 10.5937/BiljLek2306764J
ER  - 

@article{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera",
year = "2023",
abstract = "Kruška je jedna od najrasprostranjenijih vrsta voćaka u svetu. U odnosuna koštičave i jagodaste vrste voćaka, krušku i ostale jabučaste vrste, zaražava manji broj virusa i viroida. Virusi kod većine komercijalnih sorti kruške ne izazivaju simptome na lišću, plodovima i drugim biljnim organima. Kod osetljivih sorti, simptomi se javljaju samo na listovima i plodovima. Široko rasprostanjeni i ekonomski značajni virusi kruške su: virus jamičavosti stabla jabuke (apple stem pitting virus, ASPV), virus brazdavosti stabla jabuke (apple stem grooving virus, ASGV), virus hlorotične lisne pegavosti jabuke (apple chlorotic leaf spot virus, ACLSV) i virus mozaika jabuke (apple mosaic virus, ApMV). Od viroida, po štetnosti se izdvajaju viroid mehuravih rak rana kruške (pear blister canker viroid, PBCVd) i viroid plutavosti pokožice ploda jabuke (apple scar skin viroid, ASSVd)., One of the most common fruit species in the world is the pears. Comparedto stone and small fruit trees, pears and other pome fruits are infected by a lesser number of viruses and viroids. The majority of commercial pear cultivars do not exhibit symptoms on their leaves, fruits, or other plant parts due to viruses. Only foliage and fruits show symptoms in susceptible cultivars. Apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and apple mosaic virus (ApMV) are widespread and economically significant pear viruses. Pear blister canker viroid (PBCVd) and apple scar skin viroid (ASSVd) are the most dangerous viroids infecting pears.",
publisher = "Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine",
journal = "Biljni Lekar",
title = "Virusi i viroidi kruške, Viruses and viroids infecting pears",
pages = "774-764",
number = "6",
volume = "51",
doi = "10.5937/BiljLek2306764J"
}

Jevremović, D., Vasilijević, B.,& Katanić, V.. (2023). Virusi i viroidi kruške. in Biljni Lekar
Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za fitomedicinu i zaštitu životne sredine., 51(6), 764-774.
https://doi.org/10.5937/BiljLek2306764J

Jevremović D, Vasilijević B, Katanić V. Virusi i viroidi kruške. in Biljni Lekar. 2023;51(6):764-774.
doi:10.5937/BiljLek2306764J .

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, "Virusi i viroidi kruške" in Biljni Lekar, 51, no. 6 (2023):764-774,
https://doi.org/10.5937/BiljLek2306764J . .

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Paunović, Svetlana A
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/844
AB  - Tri glavna (PPV-M, PPV-D i PPV-Rec), od deset poznatih sojeva virusa šarke šlјive
(plum pox virus, PPV) prisutno je u zasadima koštičavih vrsta voćaka u Srbiji. U zasadima šljive u našoj zemlji dominantno je prisutan PPV-Rec soj. Cilj ovog istraživanja je utvrđivanje vremenske i prostorne brzine širenja izolata PPV-Rec i PPV-D sojeva u zasadu šljive. Istraživanje je sprovedeno u oglednom zasadu šljive sorte Čačanska lepotica (400
stabala posađenih na rastojanju 4x3,5 m) koji je podignut 2008. godine. Po četiri stabla koja se nalaze u sredini zasada su veštački inokulisana sa odabranim PPV-D i PPV-Rec izolatima. Inokulisana stabla su predstavljala interni izvor infekcije. Sva stabla Prunus vrsta koja se nalaze u bližoj okolini voćnjaka su precizno mapirana, testirana na PPV i sva zaražena stabla su smatrana eksternim izvorom infekcije. Izolati PPV (interni i eksterni izvori zaraze) su delimično sekvencirani (n-ter CP region genoma) u cilju praćenja izvora zaraze u početnim godinama istraživanja. U periodu 2008−2023. godine sva stabla u voćnjaku su redovno vizuelno pregledana na
prisustvo simptoma i testirana IC-RT-PCR metodom na prisustvo PPV. Za detekciju i karakterizaciju izolata korišćeni su PPV-soj specifični prajmeri kojima je kroz četiri odvojene PCR reakcije omogućena karakterizacija izolata. Na početku eksperimenta relativni odnos stabala zaraženih PPV-D/PPV-Rec (uklјučujući interne i eksterne izvore) bio je 1:3. Prvi slučajevi zaraze u voćnjaku utvrđeni su 2009. godine (3 stabla), a rezultati sekvenciranja su potvrdili da zaraza potiče iz eksternih izvora. Sa intenzivnim porastom stabala, zaražena stabla u zasadu su postala primaran izvor novih infekcija koje su detektovane u narednim godinama. U 2023. godini potvrđeno je ukupno 326 zaraženih stabala: 29 PPV-D, 275 PPV-Rec i 22 stabla sa mešanom infekcijom (PPV-D+PPV-Rec). U 2023. godini relativni odnos zaraženih stabala u zasadu PPV-D/PPV-Rec je 1:9,4. Rezultati petnaestogodišnjih istraživanja su potvrdili da se PPV-Rec soj u zasadu šljive
značajno brže prenosi lisnim vašima od PPV-D soja. PPV-Rec izolati su se pokazali konkurentnijim i predstavljaju potencijalno veću opasnost za pojavu epidemija u zasadima šlјive u odnosu na PPV-D izolate.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova : XVII simpozijum o zaštiti bilja,  27-30. novembar 2023., Zlatibor, Srbija
T1  - Virus šarke šljive: 15 godina praćenja širenja PPV-Rec i PPV-D sojeva u zasadu šljive
EP  - 9
SP  - 8
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Paunović, Svetlana A",
year = "2023",
abstract = "Tri glavna (PPV-M, PPV-D i PPV-Rec), od deset poznatih sojeva virusa šarke šlјive(plum pox virus, PPV) prisutno je u zasadima koštičavih vrsta voćaka u Srbiji. U zasadima šljive u našoj zemlji dominantno je prisutan PPV-Rec soj. Cilj ovog istraživanja je utvrđivanje vremenske i prostorne brzine širenja izolata PPV-Rec i PPV-D sojeva u zasadu šljive. Istraživanje je sprovedeno u oglednom zasadu šljive sorte Čačanska lepotica (400stabala posađenih na rastojanju 4x3,5 m) koji je podignut 2008. godine. Po četiri stabla koja se nalaze u sredini zasada su veštački inokulisana sa odabranim PPV-D i PPV-Rec izolatima. Inokulisana stabla su predstavljala interni izvor infekcije. Sva stabla Prunus vrsta koja se nalaze u bližoj okolini voćnjaka su precizno mapirana, testirana na PPV i sva zaražena stabla su smatrana eksternim izvorom infekcije. Izolati PPV (interni i eksterni izvori zaraze) su delimično sekvencirani (n-ter CP region genoma) u cilju praćenja izvora zaraze u početnim godinama istraživanja. U periodu 2008−2023. godine sva stabla u voćnjaku su redovno vizuelno pregledana naprisustvo simptoma i testirana IC-RT-PCR metodom na prisustvo PPV. Za detekciju i karakterizaciju izolata korišćeni su PPV-soj specifični prajmeri kojima je kroz četiri odvojene PCR reakcije omogućena karakterizacija izolata. Na početku eksperimenta relativni odnos stabala zaraženih PPV-D/PPV-Rec (uklјučujući interne i eksterne izvore) bio je 1:3. Prvi slučajevi zaraze u voćnjaku utvrđeni su 2009. godine (3 stabla), a rezultati sekvenciranja su potvrdili da zaraza potiče iz eksternih izvora. Sa intenzivnim porastom stabala, zaražena stabla u zasadu su postala primaran izvor novih infekcija koje su detektovane u narednim godinama. U 2023. godini potvrđeno je ukupno 326 zaraženih stabala: 29 PPV-D, 275 PPV-Rec i 22 stabla sa mešanom infekcijom (PPV-D+PPV-Rec). U 2023. godini relativni odnos zaraženih stabala u zasadu PPV-D/PPV-Rec je 1:9,4. Rezultati petnaestogodišnjih istraživanja su potvrdili da se PPV-Rec soj u zasadu šljiveznačajno brže prenosi lisnim vašima od PPV-D soja. PPV-Rec izolati su se pokazali konkurentnijim i predstavljaju potencijalno veću opasnost za pojavu epidemija u zasadima šlјive u odnosu na PPV-D izolate.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova : XVII simpozijum o zaštiti bilja,  27-30. novembar 2023., Zlatibor, Srbija",
title = "Virus šarke šljive: 15 godina praćenja širenja PPV-Rec i PPV-D sojeva u zasadu šljive",
pages = "9-8"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Paunović, S. A.. (2023). Virus šarke šljive: 15 godina praćenja širenja PPV-Rec i PPV-D sojeva u zasadu šljive. in Zbornik rezimea radova : XVII simpozijum o zaštiti bilja,  27-30. novembar 2023., Zlatibor, Srbija
Beograd: Društvo za zaštitu bilja Srbije., 8-9.

Jevremović D, Vasilijević B, Katanić V, Paunović SA. Virus šarke šljive: 15 godina praćenja širenja PPV-Rec i PPV-D sojeva u zasadu šljive. in Zbornik rezimea radova : XVII simpozijum o zaštiti bilja,  27-30. novembar 2023., Zlatibor, Srbija. 2023;:8-9..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Paunović, Svetlana A, "Virus šarke šljive: 15 godina praćenja širenja PPV-Rec i PPV-D sojeva u zasadu šljive" in Zbornik rezimea radova : XVII simpozijum o zaštiti bilja,  27-30. novembar 2023., Zlatibor, Srbija (2023):8-9.

TY  - JOUR
AU  - Živković, Sanja
AU  - Vasić, Tanja
AU  - Mihajlović, Biljana
AU  - Katanić, Vera
AU  - Vasilijević, Bojana
AU  - Jevremović, Darko
AU  - Debasis, Mitra
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/843
AB  - The research conducted in this study aimed to investigate the impact of retorted beech charcoal on the shoot number and length of the highbush blueberry cultivar ‘Duke’. The experimental trial was established using randomized block system, consisting of three treatments and a control group. The control plants were potted in plastic containers filled with a mixture of sawdust and a substrate designed for commercial blueberry growing by Klasman. The plants in the treatment groups were potted similarly but with the addition of retorted beech charcoal in the root zone and around the root neck. Three different quantities of charcoal were used: 150 g (T1), 100 g (T2) and 50 g (T3). The number and length of shoots were monitored over the two-year period. The results indicated a positive influence of retorted beech charcoal on the number and length of shoots. Specifically, the plants in the treatment groups exhibited longer and more numerous shoots compared to the control group. Furthermore, the results varied depending on the quantity of retorted beech charcoal applied. The treatment with 150 g of retorted beech charcoal recorded the highest average length and number of shoots. Additionally, in both the treatment and control groups, a greater number and length of shoots were observed in the second year of the study.
AB  - Istraživanja obuhvaćena ovim radom sprovedena su u cilju proučavanja uticaja primene retortnog bukovog uglja na broj i dužinu jednogodišnjih prirasta kod biljaka visokožbunaste borovnice sorte Duke. Eksperimentalni ogled bio je postavljen po dizajnu slučajnog blok sistema i uključivao je tri tretmana i kontrolu. Biljke koje su poslužile kao kontola zasađene su u plastične saksije napunjene mešavinom strugotine i komercijalnog supstrata za gajenje borovnice čiji proizvođač Klasman. Biljke iz tretmana su posađene na isti način ali uz dodatak retortnog bukovog uglja u zoni korena i korenovog vrata biljaka u količini od 150 g (T1), 100 g (T2) i 50 g (T3). Broj i dužina jednogodišnjih prirasta praćeni su tokom dve godine. Dobijeni rezultati su pokazali da retortni bukov ugalj pozitivno utiče na broj i dužinu jednogodišnjih prirasta. Naime, biljke u tretmanu su imale veću dužinu i broj jednogodišnjih prirasta u odnosu na kontrolne biljke. Utvrđene su i razlike u dobijenim rezultatima u zavisnosti od primenjene količine retortnog bukovog uglja. Najveća prosečna dužina i broj jednogodišnjih prirasta zabeleženi su u tretmanu sa 150 g retortnog bukovog uglja. Veći broj i veća dužina jednogodišnjih prirasta u svim tretmanima i kontroli zabeleženi su u drugoj godini proučavanja.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo
T1  - Cultivating organic American highbush blueberries with retorted beech charcoal (RBC)
T1  - Organska proizvodnja američke visokožbunaste borovnice korišćenjem retortnog bukovog uglja
EP  - 130
IS  - 217-218
SP  - 125
VL  - 57
DO  - https://doi.org/10.18485/pomology.2023.57.217_218.5
ER  - 

@article{
author = "Živković, Sanja and Vasić, Tanja and Mihajlović, Biljana and Katanić, Vera and Vasilijević, Bojana and Jevremović, Darko and Debasis, Mitra",
year = "2023",
abstract = "The research conducted in this study aimed to investigate the impact of retorted beech charcoal on the shoot number and length of the highbush blueberry cultivar ‘Duke’. The experimental trial was established using randomized block system, consisting of three treatments and a control group. The control plants were potted in plastic containers filled with a mixture of sawdust and a substrate designed for commercial blueberry growing by Klasman. The plants in the treatment groups were potted similarly but with the addition of retorted beech charcoal in the root zone and around the root neck. Three different quantities of charcoal were used: 150 g (T1), 100 g (T2) and 50 g (T3). The number and length of shoots were monitored over the two-year period. The results indicated a positive influence of retorted beech charcoal on the number and length of shoots. Specifically, the plants in the treatment groups exhibited longer and more numerous shoots compared to the control group. Furthermore, the results varied depending on the quantity of retorted beech charcoal applied. The treatment with 150 g of retorted beech charcoal recorded the highest average length and number of shoots. Additionally, in both the treatment and control groups, a greater number and length of shoots were observed in the second year of the study., Istraživanja obuhvaćena ovim radom sprovedena su u cilju proučavanja uticaja primene retortnog bukovog uglja na broj i dužinu jednogodišnjih prirasta kod biljaka visokožbunaste borovnice sorte Duke. Eksperimentalni ogled bio je postavljen po dizajnu slučajnog blok sistema i uključivao je tri tretmana i kontrolu. Biljke koje su poslužile kao kontola zasađene su u plastične saksije napunjene mešavinom strugotine i komercijalnog supstrata za gajenje borovnice čiji proizvođač Klasman. Biljke iz tretmana su posađene na isti način ali uz dodatak retortnog bukovog uglja u zoni korena i korenovog vrata biljaka u količini od 150 g (T1), 100 g (T2) i 50 g (T3). Broj i dužina jednogodišnjih prirasta praćeni su tokom dve godine. Dobijeni rezultati su pokazali da retortni bukov ugalj pozitivno utiče na broj i dužinu jednogodišnjih prirasta. Naime, biljke u tretmanu su imale veću dužinu i broj jednogodišnjih prirasta u odnosu na kontrolne biljke. Utvrđene su i razlike u dobijenim rezultatima u zavisnosti od primenjene količine retortnog bukovog uglja. Najveća prosečna dužina i broj jednogodišnjih prirasta zabeleženi su u tretmanu sa 150 g retortnog bukovog uglja. Veći broj i veća dužina jednogodišnjih prirasta u svim tretmanima i kontroli zabeleženi su u drugoj godini proučavanja.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo",
title = "Cultivating organic American highbush blueberries with retorted beech charcoal (RBC), Organska proizvodnja američke visokožbunaste borovnice korišćenjem retortnog bukovog uglja",
pages = "130-125",
number = "217-218",
volume = "57",
doi = "https://doi.org/10.18485/pomology.2023.57.217_218.5"
}

Živković, S., Vasić, T., Mihajlović, B., Katanić, V., Vasilijević, B., Jevremović, D.,& Debasis, M.. (2023). Cultivating organic American highbush blueberries with retorted beech charcoal (RBC). in Voćarstvo
Čačak : Naučno voćarsko društvo Srbije., 57(217-218), 125-130.
https://doi.org/https://doi.org/10.18485/pomology.2023.57.217_218.5

Živković S, Vasić T, Mihajlović B, Katanić V, Vasilijević B, Jevremović D, Debasis M. Cultivating organic American highbush blueberries with retorted beech charcoal (RBC). in Voćarstvo. 2023;57(217-218):125-130.
doi:https://doi.org/10.18485/pomology.2023.57.217_218.5 .

Živković, Sanja, Vasić, Tanja, Mihajlović, Biljana, Katanić, Vera, Vasilijević, Bojana, Jevremović, Darko, Debasis, Mitra, "Cultivating organic American highbush blueberries with retorted beech charcoal (RBC)" in Voćarstvo, 57, no. 217-218 (2023):125-130,
https://doi.org/https://doi.org/10.18485/pomology.2023.57.217_218.5 . .

TY  - JOUR
AU  - Živković, Sanja
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Katanić, Vera
AU  - Mitrović, Olga
AU  - Vasilijević, Bojana
AU  - Debasis, Mitra
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/842
AB  - Analizom korovske flore u mladim zasadima maline u selu Osreci zabeleženo je prisustvo ukupno 26 korovskih vrsta [7 korovskih (segetalnih), 13 korovsko-ruderalnih, 1 ruderalna i 5 korova livada i pašnjaka]. Većina evidentiranih korova cvetala je od juna do septembra. U konstatovanoj korovskoj flori dominirali su predstavnici klase Magnoliopsida (dikotile, širokolisni korovi) i to 24 vrste, dok su iz klase Liliopsida (monokotile, uskolisni korovi), bile prisutne samo dve vrste. Analiza životnih formi korova u zasadima maline pokazala je blagu dominaciju hemikriptofita (38,46%) u odnosu na geofite (30,77%) i terofite (26,92%). Zakorovljenost nije bila velika, jer je na svakih deset do petnaest dana vršeno međuredno kultiviranje, a u redovima, oko biljaka, su ručno odstranjivani korovi. Bez obzira na mehaničko odstranjivanje korova diverzitet korovske flore nije bio zanemarljiv. U svim posmatranim mladim zasadima maline najučestaliji korovi bili su: Salvia verticillata L., Lathyrus latifolius L., Cirsium arvense (L.) Scop., Equisetum arvense L., Pteridium aquilinum (L.) Kuhn., Polygonum convolvulus L., Convolvulus arvensis L., Chenopodium album L.
AB  - The analysis of the weed flora in the young raspberry plantations in the village of Osreci revealed the presence of a total of 26 weed species [7 weed (segetal), 13 weed-ruderal, 1 ruderal and 5 weeds of meadows and pastures]. The most of the identified weeds flowered from June to September. The recorded weed flora is dominated by representatives of the class Magnoliopsida (dicotyledons, broad-leaved weeds) with 24 species, while only two species are present from the class Liliopsida (monocotyledons, narrow-leaved weeds). The analysis of weed life forms in raspberry plantations shows a slight dominance of hemicryptophytes (38.46%) compared to geophytes (30.77%) and therophytes (26.92%). The number of weeds was not high, because inter-row cultivation was done every ten to fifteen days, and weeds were manually removed in the rows, around the plants. Regardless of the mechanical removal of weeds, the diversity of the weed flora was not negligible. In all observed young raspberry plantations, the most frequent weeds are: Salvia verticillata L., Lathyrus latifolius L., Cirsium arvense (L.) Scop., Equisetum arvense L., Pteridium aquilinum (L.) Kuhn., Polygonum convolvulus L., Convolvulus arvensis L., Chenopodium album L.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo
T1  - Floristička analiza korova u organskim zasadima maline
T1  - Floristic analysis of weeds in raspberry plantations under organic production
EP  - 124
IS  - 217-218
SP  - 117
VL  - 57
DO  - https://doi.org/10.18485/pomology.2023.57.217_218.4
ER  - 

@article{
author = "Živković, Sanja and Vasić, Tanja and Jevremović, Darko and Katanić, Vera and Mitrović, Olga and Vasilijević, Bojana and Debasis, Mitra",
year = "2023",
abstract = "Analizom korovske flore u mladim zasadima maline u selu Osreci zabeleženo je prisustvo ukupno 26 korovskih vrsta [7 korovskih (segetalnih), 13 korovsko-ruderalnih, 1 ruderalna i 5 korova livada i pašnjaka]. Većina evidentiranih korova cvetala je od juna do septembra. U konstatovanoj korovskoj flori dominirali su predstavnici klase Magnoliopsida (dikotile, širokolisni korovi) i to 24 vrste, dok su iz klase Liliopsida (monokotile, uskolisni korovi), bile prisutne samo dve vrste. Analiza životnih formi korova u zasadima maline pokazala je blagu dominaciju hemikriptofita (38,46%) u odnosu na geofite (30,77%) i terofite (26,92%). Zakorovljenost nije bila velika, jer je na svakih deset do petnaest dana vršeno međuredno kultiviranje, a u redovima, oko biljaka, su ručno odstranjivani korovi. Bez obzira na mehaničko odstranjivanje korova diverzitet korovske flore nije bio zanemarljiv. U svim posmatranim mladim zasadima maline najučestaliji korovi bili su: Salvia verticillata L., Lathyrus latifolius L., Cirsium arvense (L.) Scop., Equisetum arvense L., Pteridium aquilinum (L.) Kuhn., Polygonum convolvulus L., Convolvulus arvensis L., Chenopodium album L., The analysis of the weed flora in the young raspberry plantations in the village of Osreci revealed the presence of a total of 26 weed species [7 weed (segetal), 13 weed-ruderal, 1 ruderal and 5 weeds of meadows and pastures]. The most of the identified weeds flowered from June to September. The recorded weed flora is dominated by representatives of the class Magnoliopsida (dicotyledons, broad-leaved weeds) with 24 species, while only two species are present from the class Liliopsida (monocotyledons, narrow-leaved weeds). The analysis of weed life forms in raspberry plantations shows a slight dominance of hemicryptophytes (38.46%) compared to geophytes (30.77%) and therophytes (26.92%). The number of weeds was not high, because inter-row cultivation was done every ten to fifteen days, and weeds were manually removed in the rows, around the plants. Regardless of the mechanical removal of weeds, the diversity of the weed flora was not negligible. In all observed young raspberry plantations, the most frequent weeds are: Salvia verticillata L., Lathyrus latifolius L., Cirsium arvense (L.) Scop., Equisetum arvense L., Pteridium aquilinum (L.) Kuhn., Polygonum convolvulus L., Convolvulus arvensis L., Chenopodium album L.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo",
title = "Floristička analiza korova u organskim zasadima maline, Floristic analysis of weeds in raspberry plantations under organic production",
pages = "124-117",
number = "217-218",
volume = "57",
doi = "https://doi.org/10.18485/pomology.2023.57.217_218.4"
}

Živković, S., Vasić, T., Jevremović, D., Katanić, V., Mitrović, O., Vasilijević, B.,& Debasis, M.. (2023). Floristička analiza korova u organskim zasadima maline. in Voćarstvo
Čačak : Naučno voćarsko društvo Srbije., 57(217-218), 117-124.
https://doi.org/https://doi.org/10.18485/pomology.2023.57.217_218.4

Živković S, Vasić T, Jevremović D, Katanić V, Mitrović O, Vasilijević B, Debasis M. Floristička analiza korova u organskim zasadima maline. in Voćarstvo. 2023;57(217-218):117-124.
doi:https://doi.org/10.18485/pomology.2023.57.217_218.4 .

Živković, Sanja, Vasić, Tanja, Jevremović, Darko, Katanić, Vera, Mitrović, Olga, Vasilijević, Bojana, Debasis, Mitra, "Floristička analiza korova u organskim zasadima maline" in Voćarstvo, 57, no. 217-218 (2023):117-124,
https://doi.org/https://doi.org/10.18485/pomology.2023.57.217_218.4 . .

TY  - CONF
AU  - Jevremović, Darko
AU  - Zejak, Dejan
AU  - Vasilijević, Bojana
AU  - Leposavić, Aleksandar
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/787
AB  - Highbush blueberries (Vaccinium corymbosum L.) are a recent addition to Montenegro’s fruit producing repertoire. The northern region of the country is home to new, dense plantations. To investigate the presence of viruses in blueberry plantations in Montenegro
during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars were collected and analyzed. The samples were tested by ELISA for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). Assays were conducted according to the recommended protocols. The samples were further tested for the presence of blueberry mosaic-associated virus and blueberry latent virus using RT-PCR and for the presence of the blueberry red ringspot virus using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was done with pd(N)6 random primers, and PCR was done with virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide. The presence of blueberry latent virus was confirmed in 10 blueberry samples
(59%) in cultivars ‘’Duke’, ‘Bluecrop’, and ‘Earlyblue’. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples. This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.
PB  - Springer
C3  - Lecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challenges
T1  - Detection of blueberry latent virus on highbush blueberries in Montenegro
EP  - 29
SP  - 23
ER  - 

@conference{
author = "Jevremović, Darko and Zejak, Dejan and Vasilijević, Bojana and Leposavić, Aleksandar",
year = "2023",
abstract = "Highbush blueberries (Vaccinium corymbosum L.) are a recent addition to Montenegro’s fruit producing repertoire. The northern region of the country is home to new, dense plantations. To investigate the presence of viruses in blueberry plantations in Montenegroduring 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars were collected and analyzed. The samples were tested by ELISA for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). Assays were conducted according to the recommended protocols. The samples were further tested for the presence of blueberry mosaic-associated virus and blueberry latent virus using RT-PCR and for the presence of the blueberry red ringspot virus using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was done with pd(N)6 random primers, and PCR was done with virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide. The presence of blueberry latent virus was confirmed in 10 blueberry samples(59%) in cultivars ‘’Duke’, ‘Bluecrop’, and ‘Earlyblue’. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples. This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.",
publisher = "Springer",
journal = "Lecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challenges",
title = "Detection of blueberry latent virus on highbush blueberries in Montenegro",
pages = "29-23"
}

Jevremović, D., Zejak, D., Vasilijević, B.,& Leposavić, A.. (2023). Detection of blueberry latent virus on highbush blueberries in Montenegro. in Lecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challenges
Springer., 23-29.

Jevremović D, Zejak D, Vasilijević B, Leposavić A. Detection of blueberry latent virus on highbush blueberries in Montenegro. in Lecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challenges. 2023;:23-29..

Jevremović, Darko, Zejak, Dejan, Vasilijević, Bojana, Leposavić, Aleksandar, "Detection of blueberry latent virus on highbush blueberries in Montenegro" in Lecture Notes in Bioengineering : 32nd Scientific-Expert Conference of Agriculture and Food Industry, Local Food Production Systems in the Era of Global Challenges (2023):23-29.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Popović, Nenad
AU  - Katanić, Vera
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/515
AB  - Healthy and true-to-type plant planting material is essential for successful agricultural production. Vegetatively propagated species, such as fruits, are particularly endangered as many pathogens are transmitted through this type of propagation. The certification program for the production of fruit planting material in Serbia started in the mid-2000s. This was the pioneer effort in the country to establish the program and obtain plant material that satisfied all international and newly established national plant material production standards. Since 2005, a total of 26 plum cultivars were introduced into the program. The certification was based on the evaluation of the candidate clones and included the analysis of plants for the presence of prescribed pathogens and their pomological observations. As a result, a nuclear stock of 25 plums, including domestic, autochthonous, and foreign cultivars with a total number of 155 plants was obtained at the Fruit Research Institute, Čačak.
PB  - Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences
C3  - Proceedings : 5th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, Vrnjačka Banja, Serbia 29 – 30. Jun, 2023.
T1  - Achievements in the certification of plum planting material in Serbia
EP  - 32
SP  - 28
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Popović, Nenad and Katanić, Vera",
year = "2023",
abstract = "Healthy and true-to-type plant planting material is essential for successful agricultural production. Vegetatively propagated species, such as fruits, are particularly endangered as many pathogens are transmitted through this type of propagation. The certification program for the production of fruit planting material in Serbia started in the mid-2000s. This was the pioneer effort in the country to establish the program and obtain plant material that satisfied all international and newly established national plant material production standards. Since 2005, a total of 26 plum cultivars were introduced into the program. The certification was based on the evaluation of the candidate clones and included the analysis of plants for the presence of prescribed pathogens and their pomological observations. As a result, a nuclear stock of 25 plums, including domestic, autochthonous, and foreign cultivars with a total number of 155 plants was obtained at the Fruit Research Institute, Čačak.",
publisher = "Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences",
journal = "Proceedings : 5th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, Vrnjačka Banja, Serbia 29 – 30. Jun, 2023.",
title = "Achievements in the certification of plum planting material in Serbia",
pages = "32-28"
}

Jevremović, D., Vasilijević, B., Popović, N.,& Katanić, V.. (2023). Achievements in the certification of plum planting material in Serbia. in Proceedings : 5th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, Vrnjačka Banja, Serbia 29 – 30. Jun, 2023.
Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences., 28-32.

Jevremović D, Vasilijević B, Popović N, Katanić V. Achievements in the certification of plum planting material in Serbia. in Proceedings : 5th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, Vrnjačka Banja, Serbia 29 – 30. Jun, 2023.. 2023;:28-32..

Jevremović, Darko, Vasilijević, Bojana, Popović, Nenad, Katanić, Vera, "Achievements in the certification of plum planting material in Serbia" in Proceedings : 5th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, Vrnjačka Banja, Serbia 29 – 30. Jun, 2023. (2023):28-32.

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Anđelić, Tatjana
AU  - Vasilijević, Bojana
AU  - Jevremović, Darko
AU  - Engelmann, Florent
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/501
AB  - The objective of this study is to assess the suitability of vitrification cryo-plate (V cryo-plate) and dehydration cryo-plate (D cryo-plate) methods for the long-term conservation of eight autochthonous Prunus domestica L. genotypes originating from the Balkan Peninsula region. In vitro shoot tips were briefly pre-cultured for 1 day at 23 °C in the dark on a medium containing 0.3 M sucrose and then embedded in calcium alginate gel within the wells of the aluminum cryo-plates. In the V cryo-plate protocol, dehydration was carried out at room temperature using the following vitrification solutions: original plant vitrification solution 2 (PVS2) and 90% PVS2 solution (for 20 and 40 min) and plant vitrification solution 3 (PVS3) (for 60 and 80 min). In the D cryo-plate protocol, desiccation was performed for 2, 2.5, or 3 h over silica gel at 23 °C. The effect of different treatments was evaluated by monitoring the regrowth of both non-frozen and cryo-preserved explants. After cryo-preservation, five genotypes achieved regrowth rates over 40% in at least one of the applied protocols, while two genotypes showed regrowth rates of around 10%. A significant improvement in regrowth success for all genotypes using both cryo-plate methods was achieved by pre-culturing shoot tips for 7 days on a medium containing 0.5 M sucrose in complete darkness at 4 °C. Shoots regenerated from cryo-preserved explants were further monitored in vitro. By the third subculture, they had not only regained but had even exceeded the multiplication capacity (index of multiplication, length of axial, and lateral shoots) of shoots regenerated from dissection controls. Following multiplication, the cryo-preserved shoots were successfully rooted and rooting ability was assessed by monitoring the percentage of rooting, number and length of roots, and height of rooted plantlets.
PB  - Basel, Switzerland : MDPI
T2  - Plants
T1  - Cryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens
SP  - 3108
VL  - 12
DO  - 10.3390/plants12173108
ER  - 

@article{
author = "Vujović, Tatjana and Anđelić, Tatjana and Vasilijević, Bojana and Jevremović, Darko and Engelmann, Florent",
year = "2023",
abstract = "The objective of this study is to assess the suitability of vitrification cryo-plate (V cryo-plate) and dehydration cryo-plate (D cryo-plate) methods for the long-term conservation of eight autochthonous Prunus domestica L. genotypes originating from the Balkan Peninsula region. In vitro shoot tips were briefly pre-cultured for 1 day at 23 °C in the dark on a medium containing 0.3 M sucrose and then embedded in calcium alginate gel within the wells of the aluminum cryo-plates. In the V cryo-plate protocol, dehydration was carried out at room temperature using the following vitrification solutions: original plant vitrification solution 2 (PVS2) and 90% PVS2 solution (for 20 and 40 min) and plant vitrification solution 3 (PVS3) (for 60 and 80 min). In the D cryo-plate protocol, desiccation was performed for 2, 2.5, or 3 h over silica gel at 23 °C. The effect of different treatments was evaluated by monitoring the regrowth of both non-frozen and cryo-preserved explants. After cryo-preservation, five genotypes achieved regrowth rates over 40% in at least one of the applied protocols, while two genotypes showed regrowth rates of around 10%. A significant improvement in regrowth success for all genotypes using both cryo-plate methods was achieved by pre-culturing shoot tips for 7 days on a medium containing 0.5 M sucrose in complete darkness at 4 °C. Shoots regenerated from cryo-preserved explants were further monitored in vitro. By the third subculture, they had not only regained but had even exceeded the multiplication capacity (index of multiplication, length of axial, and lateral shoots) of shoots regenerated from dissection controls. Following multiplication, the cryo-preserved shoots were successfully rooted and rooting ability was assessed by monitoring the percentage of rooting, number and length of roots, and height of rooted plantlets.",
publisher = "Basel, Switzerland : MDPI",
journal = "Plants",
title = "Cryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens",
pages = "3108",
volume = "12",
doi = "10.3390/plants12173108"
}

Vujović, T., Anđelić, T., Vasilijević, B., Jevremović, D.,& Engelmann, F.. (2023). Cryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens. in Plants
Basel, Switzerland : MDPI., 12, 3108.
https://doi.org/10.3390/plants12173108

Vujović T, Anđelić T, Vasilijević B, Jevremović D, Engelmann F. Cryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens. in Plants. 2023;12:3108.
doi:10.3390/plants12173108 .

Vujović, Tatjana, Anđelić, Tatjana, Vasilijević, Bojana, Jevremović, Darko, Engelmann, Florent, "Cryopreservation of indigenous plums and monitoring of multiplication and rooting capacity of shoots obtained from cryopreserved specimens" in Plants, 12 (2023):3108,
https://doi.org/10.3390/plants12173108 . .

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Anđelić, Tatjana
AU  - Vujović, Tatjana
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/499
AB  - For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryotreated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR assay showed higher sensitivity in comparison to conventional RT-PCR.
PB  - Kragujevac : University, Faculty of Agronomy
in Čačak
C3  - Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023
T1  - Application of qPCR for plum pox virus detection during cryotherapy
EP  - 288
SP  - 283
DO  - 10.46793/SBT28.283J
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Anđelić, Tatjana and Vujović, Tatjana",
year = "2023",
abstract = "For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryotreated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR assay showed higher sensitivity in comparison to conventional RT-PCR.",
publisher = "Kragujevac : University, Faculty of Agronomyin Čačak",
journal = "Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023",
title = "Application of qPCR for plum pox virus detection during cryotherapy",
pages = "288-283",
doi = "10.46793/SBT28.283J"
}

Jevremović, D., Vasilijević, B., Anđelić, T.,& Vujović, T.. (2023). Application of qPCR for plum pox virus detection during cryotherapy. in Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023
Kragujevac : University, Faculty of Agronomy
in Čačak., 283-288.
https://doi.org/10.46793/SBT28.283J

Jevremović D, Vasilijević B, Anđelić T, Vujović T. Application of qPCR for plum pox virus detection during cryotherapy. in Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023. 2023;:283-288.
doi:10.46793/SBT28.283J .

Jevremović, Darko, Vasilijević, Bojana, Anđelić, Tatjana, Vujović, Tatjana, "Application of qPCR for plum pox virus detection during cryotherapy" in Proceedings : 1st International Symposium on Biotechnology, 17–18 March 2023 (2023):283-288,
https://doi.org/10.46793/SBT28.283J . .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Anđelić, Tatjana
AU  - Vujović, Tatjana
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/495
AB  - Conservation of plant species, particularly of those important for agriculture, has now reached a very high importance in order to ensure sustainable utilization of biological resources by preventing further losses of plant diversity. The most advanced method for plant conservation is cryopreservation. Cryotherapy that relies on cryopreservation procedure, alone or in combination with other techniques, can be used for pathogen elimination from infected plants. Viral diseases pose a significant threat to the agriculture, decreasing yields and crop quality. In this work, two cryotherapy methods (D and V cryo-plate) were evaluated for plum pox virus (PPV) eradication from autochthonous plums (Prunus domestica L.) 'Crvena Ranka' and 'Belosljiva', widely present under different local names on the Balkan peninsula. Nine different cryo-plate treatments were performed per genotype. Cryopreservation was effective in PPV elimination from variety 'Crvena Ranka' in three cryo-treatments. PPV elimination was, however, not achieved in variety 'Belosljiva' by any of the two methods (D and V-cryoplate) and treatments applied.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars
EP  - 538
IS  - 3
SP  - 529
VL  - 152
DO  - 10.1007/s11240-022-02425-y
UR  - conv_1651
ER  - 

@article{
author = "Jevremović, Darko and Vasilijević, Bojana and Anđelić, Tatjana and Vujović, Tatjana",
year = "2023",
abstract = "Conservation of plant species, particularly of those important for agriculture, has now reached a very high importance in order to ensure sustainable utilization of biological resources by preventing further losses of plant diversity. The most advanced method for plant conservation is cryopreservation. Cryotherapy that relies on cryopreservation procedure, alone or in combination with other techniques, can be used for pathogen elimination from infected plants. Viral diseases pose a significant threat to the agriculture, decreasing yields and crop quality. In this work, two cryotherapy methods (D and V cryo-plate) were evaluated for plum pox virus (PPV) eradication from autochthonous plums (Prunus domestica L.) 'Crvena Ranka' and 'Belosljiva', widely present under different local names on the Balkan peninsula. Nine different cryo-plate treatments were performed per genotype. Cryopreservation was effective in PPV elimination from variety 'Crvena Ranka' in three cryo-treatments. PPV elimination was, however, not achieved in variety 'Belosljiva' by any of the two methods (D and V-cryoplate) and treatments applied.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars",
pages = "538-529",
number = "3",
volume = "152",
doi = "10.1007/s11240-022-02425-y",
url = "conv_1651"
}

Jevremović, D., Vasilijević, B., Anđelić, T.,& Vujović, T.. (2023). Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 152(3), 529-538.
https://doi.org/10.1007/s11240-022-02425-y
conv_1651

Jevremović D, Vasilijević B, Anđelić T, Vujović T. Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars. in Plant Cell Tissue and Organ Culture. 2023;152(3):529-538.
doi:10.1007/s11240-022-02425-y
conv_1651 .

Jevremović, Darko, Vasilijević, Bojana, Anđelić, Tatjana, Vujović, Tatjana, "Effect of D and V cryo-plate methods for plum pox virus eradication from two plum cultivars" in Plant Cell Tissue and Organ Culture, 152, no. 3 (2023):529-538,
https://doi.org/10.1007/s11240-022-02425-y .,
conv_1651 .

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Paunović, Svetlana A.
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/560
AB  - Little cherry virus 1 (LChV-1, genus Velarivirus) and little cherry virus 2 (LChV-2, genus
Ampelovirus), members of the family Closteroviridae, are considered important cherry pathogens
worldwide. In susceptible sweet and sour cherry cultivars, LChV-1 and LChV-2 can cause severe
yield losses and reduce fruit quality. Beside cherries, both viruses were found in other stone fruit
species: plums, apricots, peaches, and almonds. The aim of our study was to access the
presence of LChV-1 and LChV-2 in major Prunus species cultivated in Serbia.
During 2017−2020 202 stone fruit samples (67 sweet cherries, 17 sour cherries, 53 apricots, 38
plums, and 27 peaches) were collected from several regions of Serbia. All samples were analyzed
with reverse transcription polymerase chain reaction (RT-PCR). TNA was extracted from fresh
leaves with a 2% CTAB buffer. Reverse transcription was performed using random hexamer
primers. PCR reactions were performed using the LCV1U/LCV1L primer pair that amplify a 419 bp
fragment of the LChV-1 3' non-translated region and LCV2UP2/LCV2LO2 for the amplification of
the 438-bp fragment of the LChV-2 methyltransferase gene, respectively. PCR products were
analyzed by agarose gel electrophoresis. Selected isolates of both viruses (10 LChV-1, and one
LChV-2) were sequenced and phylogenetically analyzed.
The presence of LChV-1 was confirmed in 18 analyzed samples (8.9%), and LChV-2 in only one
sweet cherry sample (0.5%). LChV-1 was detected in 10 sweet cherries, five apricots, and three
plums. Analyzed viruses were not detected in any of the tested sour cherry and peach samples.
Serbian LChV-1 isolates showed 76.10–100% nucleotide (nt) identity. A phylogenetic analysis
identified Serbian LChV-1 isolates from plums and sweet cherries as members of the G5
phylogenetic group. Two apricot isolates belong to the G3 phylogenetic group and one isolate to
the G5 group.
This report describes the first report of little cherry virus 1 infecting plums in Serbia and confirms
the presence of isolates from G3 and G5 phylogroups in Serbian stone fruit orchards. Out of all
tested isolates, little cherry virus 2 was confirmed in only one sweet cherry tree.
PB  - Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health
C3  - Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
T1  - Occurrence of two little cherry viruses in stone fruits in Serbia
EP  - 78
SP  - 78
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Paunović, Svetlana A.",
year = "2023",
abstract = "Little cherry virus 1 (LChV-1, genus Velarivirus) and little cherry virus 2 (LChV-2, genusAmpelovirus), members of the family Closteroviridae, are considered important cherry pathogensworldwide. In susceptible sweet and sour cherry cultivars, LChV-1 and LChV-2 can cause severeyield losses and reduce fruit quality. Beside cherries, both viruses were found in other stone fruitspecies: plums, apricots, peaches, and almonds. The aim of our study was to access thepresence of LChV-1 and LChV-2 in major Prunus species cultivated in Serbia.During 2017−2020 202 stone fruit samples (67 sweet cherries, 17 sour cherries, 53 apricots, 38plums, and 27 peaches) were collected from several regions of Serbia. All samples were analyzedwith reverse transcription polymerase chain reaction (RT-PCR). TNA was extracted from freshleaves with a 2% CTAB buffer. Reverse transcription was performed using random hexamerprimers. PCR reactions were performed using the LCV1U/LCV1L primer pair that amplify a 419 bpfragment of the LChV-1 3' non-translated region and LCV2UP2/LCV2LO2 for the amplification ofthe 438-bp fragment of the LChV-2 methyltransferase gene, respectively. PCR products wereanalyzed by agarose gel electrophoresis. Selected isolates of both viruses (10 LChV-1, and oneLChV-2) were sequenced and phylogenetically analyzed.The presence of LChV-1 was confirmed in 18 analyzed samples (8.9%), and LChV-2 in only onesweet cherry sample (0.5%). LChV-1 was detected in 10 sweet cherries, five apricots, and threeplums. Analyzed viruses were not detected in any of the tested sour cherry and peach samples.Serbian LChV-1 isolates showed 76.10–100% nucleotide (nt) identity. A phylogenetic analysisidentified Serbian LChV-1 isolates from plums and sweet cherries as members of the G5phylogenetic group. Two apricot isolates belong to the G3 phylogenetic group and one isolate tothe G5 group.This report describes the first report of little cherry virus 1 infecting plums in Serbia and confirmsthe presence of isolates from G3 and G5 phylogroups in Serbian stone fruit orchards. Out of alltested isolates, little cherry virus 2 was confirmed in only one sweet cherry tree.",
publisher = "Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health",
journal = "Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023",
title = "Occurrence of two little cherry viruses in stone fruits in Serbia",
pages = "78-78"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Paunović, S. A.. (2023). Occurrence of two little cherry viruses in stone fruits in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health., 78-78.

Jevremović D, Vasilijević B, Katanić V, Paunović SA. Occurrence of two little cherry viruses in stone fruits in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023. 2023;:78-78..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Paunović, Svetlana A., "Occurrence of two little cherry viruses in stone fruits in Serbia" in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023 (2023):78-78.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Paunović, Svetlana A.
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/559
AB  - Stone fruits (plums, apricots, peaches, and cherries) are economically important species for Serbian agriculture. These fruits harbor numerous viruses that can decrease yield and fruit quality. Cherry virus A (CVA), a member of the genus Capillovirus, was discovered during the cloning of the little cherry agent. CVA is widely distributed in more than 10 countries in sweet and sour cherries, but also in other Prunus hosts. So far, CVA has been confirmed in Serbia in sweet and sour cherries. A total of 106 leaf samples from 31 orchards in 22 locations were collected and examined to determine the prevalence of CVA in stone fruit orchards in Serbia. Great majority of leaf samples were symptomless, but some exhibited symptoms of chlorotic mottling, necrotic spots, and vein necrosis. Samples were tested by reverse transcription polymerase chain reaction (RT-PCR) using a virus-specific primer pair CVA-fw1/CVA-rev1 that amplify a fragment of the movement protein (MP) gene. PCR products were analyzed by electrophoresis in 1.5% agarose gel and stained with ethidium-bromide. To further access diversity of the detected isolates, we sequenced a partial MP of four detected isolates. The results of the RT-PCR revealed that 13 samples (12.3%) were infected with CVA. An expected 302 bp fragment was obtained in eight tested samples of sweet cherry, three apricot, and two plums. No positive amplification was evidenced in analyzed sour cherry and peach samples. CVA was detected in both asymptomatic and symptomatic samples. In all positive samples with symptoms, mixed infection with other viruses (prune dwarf virus, little cherry virus 1, and cherry green ring mottle virus) was detected. It is still unclear whether CVA is associated with any of the observed symptoms. The nucleotide sequences of the isolates showed 89.7–99.6% identity. To the best of our knowledge this is the first report of cherry virus A in apricots and plums in Serbia.
PB  - Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health
C3  - Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
T1  - Molecular detection and characterization of cherry virus a in Prunus species in Serbia
EP  - 79
SP  - 79
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Paunović, Svetlana A.",
year = "2023",
abstract = "Stone fruits (plums, apricots, peaches, and cherries) are economically important species for Serbian agriculture. These fruits harbor numerous viruses that can decrease yield and fruit quality. Cherry virus A (CVA), a member of the genus Capillovirus, was discovered during the cloning of the little cherry agent. CVA is widely distributed in more than 10 countries in sweet and sour cherries, but also in other Prunus hosts. So far, CVA has been confirmed in Serbia in sweet and sour cherries. A total of 106 leaf samples from 31 orchards in 22 locations were collected and examined to determine the prevalence of CVA in stone fruit orchards in Serbia. Great majority of leaf samples were symptomless, but some exhibited symptoms of chlorotic mottling, necrotic spots, and vein necrosis. Samples were tested by reverse transcription polymerase chain reaction (RT-PCR) using a virus-specific primer pair CVA-fw1/CVA-rev1 that amplify a fragment of the movement protein (MP) gene. PCR products were analyzed by electrophoresis in 1.5% agarose gel and stained with ethidium-bromide. To further access diversity of the detected isolates, we sequenced a partial MP of four detected isolates. The results of the RT-PCR revealed that 13 samples (12.3%) were infected with CVA. An expected 302 bp fragment was obtained in eight tested samples of sweet cherry, three apricot, and two plums. No positive amplification was evidenced in analyzed sour cherry and peach samples. CVA was detected in both asymptomatic and symptomatic samples. In all positive samples with symptoms, mixed infection with other viruses (prune dwarf virus, little cherry virus 1, and cherry green ring mottle virus) was detected. It is still unclear whether CVA is associated with any of the observed symptoms. The nucleotide sequences of the isolates showed 89.7–99.6% identity. To the best of our knowledge this is the first report of cherry virus A in apricots and plums in Serbia.",
publisher = "Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health",
journal = "Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023",
title = "Molecular detection and characterization of cherry virus a in Prunus species in Serbia",
pages = "79-79"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Paunović, S. A.. (2023). Molecular detection and characterization of cherry virus a in Prunus species in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health., 79-79.

Jevremović D, Vasilijević B, Katanić V, Paunović SA. Molecular detection and characterization of cherry virus a in Prunus species in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023. 2023;:79-79..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Paunović, Svetlana A., "Molecular detection and characterization of cherry virus a in Prunus species in Serbia" in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023 (2023):79-79.

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Anđelić, Tatjana
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
PY  - 2022
AB  - Krioprezervacija predstavlja savremenu metodu za dugotrajno čuvanje biljnog materijala na ultra niskim temperaturama (tečni azot, -196 °C). Ova metoda je komplementarna konvencionalnim metodama čuvanja jer značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima (ex situ). Program primene ove strategije za dugotrajno očuvanje biljnih genetičkih resursa iniciran je i u Institutu za voćarstvo, Čaćak u okviru Odeljenja za fiziologiju voćaka pre više od jedne decenije. Istraživanja su fokusirana na optimizaciju protokola za različite in vitro tehnike krioprezervacije koje se baziraju na direktnom potapanju biljnog materijala u tečni azot i njihovu primenu u čuvanju genetičkih resursa kontinentalnih voćaka. Primenjene tehnike podrazumavaju primenu hemijske (vitrifikacija, „droplet“ vitrifikacija, „V cryo-plate“) ili fizičke dehidratacije (inkapsulacija-dehidracija, „D cryo-plate“) biljnog materijala pre potapanja u tečni azot. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike i sorte kupine Čaćanska bestrna. Ispitivanje primene tehnike vitrifikacija kod vrsta roda Prunus i Malus pokazalo je da se značajno povećanje kapaciteta regeneracije vrhova izdanaka podloge za trešnju Gisela 5 i sorte jabuke Gala Must posle otapanja iz tečnog azota može postići variranjem dužine dehidratacije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije postignuta je ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidratacije i dužine osmotske rehidratacije kod predstavnika rodova Prunus (autohtone sorte šljive Crvena Ranka, Sitnica i Požegača, džanarika, podloga za tešnju Gisela 5), Malus (jabuka Gala Must) i Rubus (kupina Čačanska bestrna). Poslednjih godina, inicirana su takođe i istraživanja primene novorazvijenih „V cryo-plate“ i „D cryoplate“ tehnika krioprezervacije. Ove tehnike omogućavaju prevazila`enje izvesnih ograničenja vezanih za nedovoljnu dehidrataciju biljnog tkiva, oštećenje i gubitak biljnog materijala tokom pripreme za krioprezervacione protokole, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem navedenih tehnika uspešno su krioprezervirani vrhovi izdanaka različitih genotipova autohtonih šjiva (Crvena Ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge za trešnju Gisela 5, sorte jagode Clery, borovnice Toro i mongolske borovnice (Amelanchier alnifolia) koji su gajeni u uslovima kulture in vitro. Dosadašnji rezultati postignuti u ovim istraživanjima predstavljaju značajan doprinos uspostavljanju optimizirane tehnologije dugotrajnog čuvanja biljnog materijala primenom različitih tehnika krioprezervacije. Ovaj pregled istraživanja daje dobru osnovu za dalju optimizaciju protokola koje će biti moguće direktno primeniti u budući program uspostavljanja in vitro banke gena (germplazme) voćnih biljnih vrsta koje se gaje u Srbiji., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’, and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods. Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.
AB  - Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’,
and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods.
Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo
T1  - Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak
T1  - Long-term conservation of fruit tree germplasm using different in vitro cryopreservation techniques at Fruit Research Institute, Čačak
EP  - 92
IS  - 213-214
SP  - 79
VL  - 56
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Anđelić, Tatjana and Jevremović, Darko and Vasilijević, Bojana",
year = "2022",
abstract = "Krioprezervacija predstavlja savremenu metodu za dugotrajno čuvanje biljnog materijala na ultra niskim temperaturama (tečni azot, -196 °C). Ova metoda je komplementarna konvencionalnim metodama čuvanja jer značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima (ex situ). Program primene ove strategije za dugotrajno očuvanje biljnih genetičkih resursa iniciran je i u Institutu za voćarstvo, Čaćak u okviru Odeljenja za fiziologiju voćaka pre više od jedne decenije. Istraživanja su fokusirana na optimizaciju protokola za različite in vitro tehnike krioprezervacije koje se baziraju na direktnom potapanju biljnog materijala u tečni azot i njihovu primenu u čuvanju genetičkih resursa kontinentalnih voćaka. Primenjene tehnike podrazumavaju primenu hemijske (vitrifikacija, „droplet“ vitrifikacija, „V cryo-plate“) ili fizičke dehidratacije (inkapsulacija-dehidracija, „D cryo-plate“) biljnog materijala pre potapanja u tečni azot. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike i sorte kupine Čaćanska bestrna. Ispitivanje primene tehnike vitrifikacija kod vrsta roda Prunus i Malus pokazalo je da se značajno povećanje kapaciteta regeneracije vrhova izdanaka podloge za trešnju Gisela 5 i sorte jabuke Gala Must posle otapanja iz tečnog azota može postići variranjem dužine dehidratacije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije postignuta je ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidratacije i dužine osmotske rehidratacije kod predstavnika rodova Prunus (autohtone sorte šljive Crvena Ranka, Sitnica i Požegača, džanarika, podloga za tešnju Gisela 5), Malus (jabuka Gala Must) i Rubus (kupina Čačanska bestrna). Poslednjih godina, inicirana su takođe i istraživanja primene novorazvijenih „V cryo-plate“ i „D cryoplate“ tehnika krioprezervacije. Ove tehnike omogućavaju prevazila`enje izvesnih ograničenja vezanih za nedovoljnu dehidrataciju biljnog tkiva, oštećenje i gubitak biljnog materijala tokom pripreme za krioprezervacione protokole, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem navedenih tehnika uspešno su krioprezervirani vrhovi izdanaka različitih genotipova autohtonih šjiva (Crvena Ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge za trešnju Gisela 5, sorte jagode Clery, borovnice Toro i mongolske borovnice (Amelanchier alnifolia) koji su gajeni u uslovima kulture in vitro. Dosadašnji rezultati postignuti u ovim istraživanjima predstavljaju značajan doprinos uspostavljanju optimizirane tehnologije dugotrajnog čuvanja biljnog materijala primenom različitih tehnika krioprezervacije. Ovaj pregled istraživanja daje dobru osnovu za dalju optimizaciju protokola koje će biti moguće direktno primeniti u budući program uspostavljanja in vitro banke gena (germplazme) voćnih biljnih vrsta koje se gaje u Srbiji., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’, and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods. Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’,and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods.Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo",
title = "Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak, Long-term conservation of fruit tree germplasm using different in vitro cryopreservation techniques at Fruit Research Institute, Čačak",
pages = "92-79",
number = "213-214",
volume = "56"
}

Vujović, T., Ružić, Đ., Anđelić, T., Jevremović, D.,& Vasilijević, B.. (2022). Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak. in Voćarstvo
Čačak : Naučno voćarsko društvo Srbije., 56(213-214), 79-92.

Vujović T, Ružić Đ, Anđelić T, Jevremović D, Vasilijević B. Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak. in Voćarstvo. 2022;56(213-214):79-92..

Vujović, Tatjana, Ružić, Đurđina, Anđelić, Tatjana, Jevremović, Darko, Vasilijević, Bojana, "Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak" in Voćarstvo, 56, no. 213-214 (2022):79-92.

TY  - PAT
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Živković, Sanja
AU  - Vasilijević, Bojana
AU  - Marković, Jordan
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/568
AB  - A strain of the phytopathogenic fungus Neopestalotiopsis clavispora was detected and completely morphologically and molecularly characterized in the Phytopathology Laboratory of the Fruit Research Institute, Čačak. The strain is deposited in the Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands, CBS strain database (strain RS-PC-67, #CBS 149249), as the only strain of this species from Serbia. It is used as one of the reference isolates of N. clavispora for the study of phytopathogenic fungi from this genus from 2022 at the Fruit Research Institute, Čačak and at the Faculty of Agriculture of the University of Belgrade. At the international level, the strain was commercially realized in 2022.
T1  - RS-PC-67 : Strain Neopestaloptiopsis clavispora
ER  - 

@misc{
author = "Vasić, Tanja and Jevremović, Darko and Živković, Sanja and Vasilijević, Bojana and Marković, Jordan",
year = "2022",
abstract = "A strain of the phytopathogenic fungus Neopestalotiopsis clavispora was detected and completely morphologically and molecularly characterized in the Phytopathology Laboratory of the Fruit Research Institute, Čačak. The strain is deposited in the Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands, CBS strain database (strain RS-PC-67, #CBS 149249), as the only strain of this species from Serbia. It is used as one of the reference isolates of N. clavispora for the study of phytopathogenic fungi from this genus from 2022 at the Fruit Research Institute, Čačak and at the Faculty of Agriculture of the University of Belgrade. At the international level, the strain was commercially realized in 2022.",
title = "RS-PC-67 : Strain Neopestaloptiopsis clavispora"
}

Vasić, T., Jevremović, D., Živković, S., Vasilijević, B.,& Marković, J.. (2022). RS-PC-67 : Strain Neopestaloptiopsis clavispora. .

Vasić T, Jevremović D, Živković S, Vasilijević B, Marković J. RS-PC-67 : Strain Neopestaloptiopsis clavispora. 2022;..

Vasić, Tanja, Jevremović, Darko, Živković, Sanja, Vasilijević, Bojana, Marković, Jordan, "RS-PC-67 : Strain Neopestaloptiopsis clavispora" (2022).

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/519
AB  - Peach latent mosaic viroid (PLMVd) is economically the most important viroid
infecting stone fruits. In sensitive peach cultivars PLMVd induces mosaic and
calico on the leaves and deformed fruits with cracked suture. Early and accurate
detection of PLMVd is one of the main measures for its control. In our study we
have evaluated the efficiency of RT-PCR and quantitative Real-time PCR methods
for the detection of PLMVd isolates collected from peach and nectarine orchards
in Serbia. RT-PCR assay proved to be efficient for PLMVd detection, but some of
the isolates were on the limit of detection. Real-time RCR was characterized by
higher sensitivity in the detection of all tested isolates. Both assays can be
recommended for use, but for testing the material within the certification of peach
planting material Real-time PCR should be used.
PB  - Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences
C3  - Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia
T1  - Detection of peach latent mosaic viroid by RT-PCR and real-time PCR
EP  - 316
SP  - 309
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana",
year = "2022",
abstract = "Peach latent mosaic viroid (PLMVd) is economically the most important viroidinfecting stone fruits. In sensitive peach cultivars PLMVd induces mosaic andcalico on the leaves and deformed fruits with cracked suture. Early and accuratedetection of PLMVd is one of the main measures for its control. In our study wehave evaluated the efficiency of RT-PCR and quantitative Real-time PCR methodsfor the detection of PLMVd isolates collected from peach and nectarine orchardsin Serbia. RT-PCR assay proved to be efficient for PLMVd detection, but some ofthe isolates were on the limit of detection. Real-time RCR was characterized byhigher sensitivity in the detection of all tested isolates. Both assays can berecommended for use, but for testing the material within the certification of peachplanting material Real-time PCR should be used.",
publisher = "Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences",
journal = "Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia",
title = "Detection of peach latent mosaic viroid by RT-PCR and real-time PCR",
pages = "316-309"
}

Jevremović, D.,& Vasilijević, B.. (2022). Detection of peach latent mosaic viroid by RT-PCR and real-time PCR. in Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia
Belgrade : The Balkans Scientific Center of the Russian Academy of Natural Sciences., 309-316.

Jevremović D, Vasilijević B. Detection of peach latent mosaic viroid by RT-PCR and real-time PCR. in Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia. 2022;:309-316..

Jevremović, Darko, Vasilijević, Bojana, "Detection of peach latent mosaic viroid by RT-PCR and real-time PCR" in Proceedings : 4th International Scientific Conference Modern Trends In Agricultural Production, Rural Devalopment, Agro-economy, Cooperatives And Environmental Protection, 29–30. Jun 2022, Vrnjačka Banja, Serbia (2022):309-316.

TY  - CONF
AU  - Jevremović, Darko
AU  - Zejak, Dejan
AU  - Vasilijević, Bojana
AU  - Leposavić, Aleksandar
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/564
AB  - The highbush blueberry (Vaccinium corymbosum L.) is a relatively new fruit species for fruit growing in Montenegro. Established plantations are located in the northern part of the country.
To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars (‘Aurora’, ‘Bluecrop’, ‘Brigita Blue’, ‘Duke’, and ‘Earlyblue’) were collected and analyzed.
The samples were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). The samples were further tested for the presence of blueberry mosaic-associated virus (BlMaV) and blueberry latent virus (BlLV) using RT-PCR and for the presence of the blueberry red ringspot virus (BRRV) using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was performed using pd(N)6 random primers, and PCR reactions were performed using virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide.
The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars 'Duke', 'Bluecrop', and 'Earlyblue'. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples.
This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.
PB  - Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences
C3  - Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina
T1  - Detection of blueberry latent virus on highbush blueberries in Montenegro
EP  - 68
SP  - 68
ER  - 

@conference{
author = "Jevremović, Darko and Zejak, Dejan and Vasilijević, Bojana and Leposavić, Aleksandar",
year = "2022",
abstract = "The highbush blueberry (Vaccinium corymbosum L.) is a relatively new fruit species for fruit growing in Montenegro. Established plantations are located in the northern part of the country.To investigate the presence of viruses in blueberry plantations in Montenegro during 2022, visual inspections of plantations were carried out. Seventeen leaf samples of five cultivars (‘Aurora’, ‘Bluecrop’, ‘Brigita Blue’, ‘Duke’, and ‘Earlyblue’) were collected and analyzed.The samples were tested by enzyme-linked immunosorbent assay (ELISA) for the presence of: blueberry shock virus (BlShV), blueberry scorch virus (BlScV), blueberry shoestring virus (BSSV), blueberry leaf mottle virus (BLMoV), tomato ringspot virus (ToRSV), and tobacco ringspot virus (TRSV). The samples were further tested for the presence of blueberry mosaic-associated virus (BlMaV) and blueberry latent virus (BlLV) using RT-PCR and for the presence of the blueberry red ringspot virus (BRRV) using PCR. Extraction of total nucleic acids was performed with 2% CTAB buffer. Reverse transcription was performed using pd(N)6 random primers, and PCR reactions were performed using virus-specific primers. PCR products were analyzed by electrophoresis in a 1.5% agarose gel and stained with ethidium bromide.The presence of blueberry latent virus was confirmed in 10 blueberry samples (59%) in cultivars 'Duke', 'Bluecrop', and 'Earlyblue'. Virus identification was confirmed by sequencing the amplified fusion protein gene fragments of all 10 isolates. No other viruses were confirmed in the tested samples.This is the first survey for virus presence in blueberries in Montenegro and the first finding of blueberry latent virus in the country.",
publisher = "Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences",
journal = "Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina",
title = "Detection of blueberry latent virus on highbush blueberries in Montenegro",
pages = "68-68"
}

Jevremović, D., Zejak, D., Vasilijević, B.,& Leposavić, A.. (2022). Detection of blueberry latent virus on highbush blueberries in Montenegro. in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina
Sarajevo : University of Sarajevo Faculty of Agriculture and Food Sciences., 68-68.

Jevremović D, Zejak D, Vasilijević B, Leposavić A. Detection of blueberry latent virus on highbush blueberries in Montenegro. in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina. 2022;:68-68..

Jevremović, Darko, Zejak, Dejan, Vasilijević, Bojana, Leposavić, Aleksandar, "Detection of blueberry latent virus on highbush blueberries in Montenegro" in Book of Abstracts : 32nd International Scientific-Expert Conference of Agriculture and Food Industry, 1-2 December 2022, Sarajevo, Bosnia and Herzegovina (2022):68-68.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Paunović, Svetlana A.
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/775
AB  - Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 11 viruses (plum pox virus, prune dwarf virus, prunus necrotic ringspot virus, apple mosaic virus, cherry leaf roll virus, cherry necrotic rusty mottle virus, cherry rasp leaf virus, little cherry virus-1, little cherry virus-2, tomato black ring virus and tomato ringspot virus) and peach latent mosaic viroid. Molecular RT-PCR test was used for the detection of each pathogen with virus/viroid-specific primers. Analysis confirmed the presence of plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of peach latent mosaic viroid infection was 5.9%. No other viruses were detected in collected samples.
PB  - Limassol: Cyprus University of Technology & Aristotle University of Thessaloniki
C3  - Book of Аbstracts : 10th International Peach Symposium, 30 May-3 June,  2022, Naoussa, Greece
T1  - Viruses and viroids infecting peaches in Serbia
EP  - 114
SP  - 114
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Paunović, Svetlana A.",
year = "2022",
abstract = "Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 11 viruses (plum pox virus, prune dwarf virus, prunus necrotic ringspot virus, apple mosaic virus, cherry leaf roll virus, cherry necrotic rusty mottle virus, cherry rasp leaf virus, little cherry virus-1, little cherry virus-2, tomato black ring virus and tomato ringspot virus) and peach latent mosaic viroid. Molecular RT-PCR test was used for the detection of each pathogen with virus/viroid-specific primers. Analysis confirmed the presence of plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of peach latent mosaic viroid infection was 5.9%. No other viruses were detected in collected samples.",
publisher = "Limassol: Cyprus University of Technology & Aristotle University of Thessaloniki",
journal = "Book of Аbstracts : 10th International Peach Symposium, 30 May-3 June,  2022, Naoussa, Greece",
title = "Viruses and viroids infecting peaches in Serbia",
pages = "114-114"
}

Jevremović, D., Vasilijević, B.,& Paunović, S. A.. (2022). Viruses and viroids infecting peaches in Serbia. in Book of Аbstracts : 10th International Peach Symposium, 30 May-3 June,  2022, Naoussa, Greece
Limassol: Cyprus University of Technology & Aristotle University of Thessaloniki., 114-114.

Jevremović D, Vasilijević B, Paunović SA. Viruses and viroids infecting peaches in Serbia. in Book of Аbstracts : 10th International Peach Symposium, 30 May-3 June,  2022, Naoussa, Greece. 2022;:114-114..

Jevremović, Darko, Vasilijević, Bojana, Paunović, Svetlana A., "Viruses and viroids infecting peaches in Serbia" in Book of Аbstracts : 10th International Peach Symposium, 30 May-3 June,  2022, Naoussa, Greece (2022):114-114.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Paunović, Svetlana A.
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/476
AB  - Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 12 viruses (Plum pox virus, Prune dwarf virus, Prunus necrotic ringspot virus, Apple chlorotic leafspot virus, Apple mosaic virus, Cherry leaf roll virus, Cherry necrotic rusty mottle virus, Cherry rasp leaf virus, Little cherry virus-1, Little cherry virus-2, Tomato black ring virus and Tomato ringspot virus) and Peach latent mosaic viroid. DAS-ELISA and molecular RT-PCR test were used for the detection with virus/viroid-specific primers. Analysis confirmed the presence of Plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of Peach latent mosaic viroid infection was 5,9%. No other viruses were detected among the collected samples. This study confirmed very low infection rate of viruses and viroids in tested peach samples.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Viruses and viroids infecting peaches in Serbia
EP  - 500
SP  - 495
VL  - 1352
DO  - 10.17660/ActaHortic.2022.1352.67
UR  - conv_1656
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Paunović, Svetlana A.",
year = "2022",
abstract = "Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 12 viruses (Plum pox virus, Prune dwarf virus, Prunus necrotic ringspot virus, Apple chlorotic leafspot virus, Apple mosaic virus, Cherry leaf roll virus, Cherry necrotic rusty mottle virus, Cherry rasp leaf virus, Little cherry virus-1, Little cherry virus-2, Tomato black ring virus and Tomato ringspot virus) and Peach latent mosaic viroid. DAS-ELISA and molecular RT-PCR test were used for the detection with virus/viroid-specific primers. Analysis confirmed the presence of Plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of Peach latent mosaic viroid infection was 5,9%. No other viruses were detected among the collected samples. This study confirmed very low infection rate of viruses and viroids in tested peach samples.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Viruses and viroids infecting peaches in Serbia",
pages = "500-495",
volume = "1352",
doi = "10.17660/ActaHortic.2022.1352.67",
url = "conv_1656"
}

Jevremović, D., Vasilijević, B.,& Paunović, S. A.. (2022). Viruses and viroids infecting peaches in Serbia. in Acta Horticulturae
International Society for Horticultural Science., 1352, 495-500.
https://doi.org/10.17660/ActaHortic.2022.1352.67
conv_1656

Jevremović D, Vasilijević B, Paunović SA. Viruses and viroids infecting peaches in Serbia. in Acta Horticulturae. 2022;1352:495-500.
doi:10.17660/ActaHortic.2022.1352.67
conv_1656 .

Jevremović, Darko, Vasilijević, Bojana, Paunović, Svetlana A., "Viruses and viroids infecting peaches in Serbia" in Acta Horticulturae, 1352 (2022):495-500,
https://doi.org/10.17660/ActaHortic.2022.1352.67 .,
conv_1656 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Leposavić, Aleksandar
AU  - Vasilijević, Bojana
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/520
AB  - The most important berry fruits produced in Serbia are raspberries, blackberries, strawberries and blueberries, while currants and others are of less importance. Berry fruits are propagated in a vegetative manner and are prone to attack by numerous fungi, bacteria and viruses. Diseases caused by viruses are the most difficult to control. Viruses may cause great crop losses in sensitive cultivars, resulting in low yields and poor fruit quality. In single infections, numerous viruses cause latent infections and no damages, but in mixed infections can lead to significant disease severity. Extensive research of viruses infecting berry fruits in Serbia has started during 2000s in the Fruit Research Institute in Čačak. The development of molecular techniques and their application for detection and characterization of pathogens have led to the identifications of many new viruses in the country. Raspberry leaf blotch virus, black raspberry necrosis virus, Rubus yellow net virus, raspberry vein chlorosis virus, raspberry mottle virus and raspberry latent virus were identified in raspberry orchards. In blueberries, blueberry red ringspot virus, blueberry mosaicassociated virus and blueberry latent virus were reported in the last decade. No new viruses in blackberries have been found, and the research of the strawberry viruses is still negligible. In this paper, the detailed synthesis of the results obtained on berry fruit viral diseases in Serbia achieved over the past two decades is presented.
AB  - Най-важните ягодоплодни видове, произвеждани в Сърбия, са малини, къпини, ягоди и боровинки, докато касисът и други са с по-малко значение. Ягодоплодните култури се размножават по вегетативен начин и са податливи на нападение от множество гъби, бактерии и вируси. Болестите, причинени от вируси, са най-трудни за контролиране. Вирусите могат да причинят големи загуби за реколтата при чувствителни сортове, което води до ниски добиви и лошо качество на плодовете. При единични инфекции, множество вируси причиняват латентни инфекции без вреда, но при смесени инфекции могат да доведат до значителна тежест на заболяването. Обширните изследвания, на вируси по ягодоплодните култури в Сърбия, започнаха в началото на 2000 та година в Изследователски институт по овощарство в Чачак. Развитието на молекулярните технологии и тяхното
приложение за откриване и характеризиране на патогени доведе до установяването на много нови вируси в страната. В малиновите градини са открити следните вируси: листни петна по малината, върхова некроза на черна малина, жълтеница, жилкова хлороза, мозаечен вирус и латентни петна. При боровинките през последното десетилетие са посочени следните вируси: червени пръстеновидни петна, мозаечен вирус и латентни петна. Не са открити нови вируси в къпините, а изследванията на вирусите на ягодите са все още незначителни.
В настоящата статия е представен и обсъден подробен синтез на резултати, получени за вирусните заболявания на ягодоплодните култури в Сърбия, постигнати през последните две десетилетия.
PB  - Troyan : Research Institute of Mountain Stockbreeding and Agriculture
T2  - Journal of Mountain Agriculture on the Balkans
T1  - Virus diseases of berry fruits in Serbia
T1  - Вирусни заболявания по ягодоплодните култури в Сърбия
EP  - 146
IS  - 3
SP  - 133
VL  - 25
ER  - 

@article{
author = "Jevremović, Darko and Leposavić, Aleksandar and Vasilijević, Bojana",
year = "2022",
abstract = "The most important berry fruits produced in Serbia are raspberries, blackberries, strawberries and blueberries, while currants and others are of less importance. Berry fruits are propagated in a vegetative manner and are prone to attack by numerous fungi, bacteria and viruses. Diseases caused by viruses are the most difficult to control. Viruses may cause great crop losses in sensitive cultivars, resulting in low yields and poor fruit quality. In single infections, numerous viruses cause latent infections and no damages, but in mixed infections can lead to significant disease severity. Extensive research of viruses infecting berry fruits in Serbia has started during 2000s in the Fruit Research Institute in Čačak. The development of molecular techniques and their application for detection and characterization of pathogens have led to the identifications of many new viruses in the country. Raspberry leaf blotch virus, black raspberry necrosis virus, Rubus yellow net virus, raspberry vein chlorosis virus, raspberry mottle virus and raspberry latent virus were identified in raspberry orchards. In blueberries, blueberry red ringspot virus, blueberry mosaicassociated virus and blueberry latent virus were reported in the last decade. No new viruses in blackberries have been found, and the research of the strawberry viruses is still negligible. In this paper, the detailed synthesis of the results obtained on berry fruit viral diseases in Serbia achieved over the past two decades is presented., Най-важните ягодоплодни видове, произвеждани в Сърбия, са малини, къпини, ягоди и боровинки, докато касисът и други са с по-малко значение. Ягодоплодните култури се размножават по вегетативен начин и са податливи на нападение от множество гъби, бактерии и вируси. Болестите, причинени от вируси, са най-трудни за контролиране. Вирусите могат да причинят големи загуби за реколтата при чувствителни сортове, което води до ниски добиви и лошо качество на плодовете. При единични инфекции, множество вируси причиняват латентни инфекции без вреда, но при смесени инфекции могат да доведат до значителна тежест на заболяването. Обширните изследвания, на вируси по ягодоплодните култури в Сърбия, започнаха в началото на 2000 та година в Изследователски институт по овощарство в Чачак. Развитието на молекулярните технологии и тяхнотоприложение за откриване и характеризиране на патогени доведе до установяването на много нови вируси в страната. В малиновите градини са открити следните вируси: листни петна по малината, върхова некроза на черна малина, жълтеница, жилкова хлороза, мозаечен вирус и латентни петна. При боровинките през последното десетилетие са посочени следните вируси: червени пръстеновидни петна, мозаечен вирус и латентни петна. Не са открити нови вируси в къпините, а изследванията на вирусите на ягодите са все още незначителни.В настоящата статия е представен и обсъден подробен синтез на резултати, получени за вирусните заболявания на ягодоплодните култури в Сърбия, постигнати през последните две десетилетия.",
publisher = "Troyan : Research Institute of Mountain Stockbreeding and Agriculture",
journal = "Journal of Mountain Agriculture on the Balkans",
title = "Virus diseases of berry fruits in Serbia, Вирусни заболявания по ягодоплодните култури в Сърбия",
pages = "146-133",
number = "3",
volume = "25"
}

Jevremović, D., Leposavić, A.,& Vasilijević, B.. (2022). Virus diseases of berry fruits in Serbia. in Journal of Mountain Agriculture on the Balkans
Troyan : Research Institute of Mountain Stockbreeding and Agriculture., 25(3), 133-146.

Jevremović D, Leposavić A, Vasilijević B. Virus diseases of berry fruits in Serbia. in Journal of Mountain Agriculture on the Balkans. 2022;25(3):133-146..

Jevremović, Darko, Leposavić, Aleksandar, Vasilijević, Bojana, "Virus diseases of berry fruits in Serbia" in Journal of Mountain Agriculture on the Balkans, 25, no. 3 (2022):133-146.

TY  - CONF
AU  - Vujović, Tatjana
AU  - Jevremović, Darko
AU  - Anđelić, Tatjana
AU  - Vasilijević, Bojana
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/498
AB  - Fast and cost-effective clonal propagation of planting material is possible to achieve by application of tissue culture in vitro. In this way, problems associated with traditional propagation, such as rapid spreading of diseases, lack of initial material caused by dependence on seasonal growth or low propagation coefficient can be overcome. This paper deals with optimization of micropropagation of autochthonous plum ‘Metlaš’ (Prunus domestica L.) originated from Guberevci (Municipality Knić, Serbia). ‘Metlaš’ is very often considered to be the same genotype as ‘Okruglica’ (syn. ‘Dragačica’), but they can be clearly distinguished according to plant habit, stone and fruit characteristics. To optimize multiplication stage, the influence of benzyladenine (BA at 0.5, 1.0, 1.5 and 2.0 mg/l) and thidiazuron (TDZ at 0.25, 0.5, 1.0 and 1.5 mg/l) on the multiplication capacity (multiplication index, length of axial and lateral shoots) was examined. Rooting ability of shoots (rooting rate, number and length of roots, and height of rooted plants) was monitored on half strength Murashige and Skoog (MS) medium containing 1.0 mg/l indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA), each combined with 0.1 mg/l gibberellic acid (GA3). TDZ applied at 0.25 mg/l gave the highest multiplication index (9.1), while the longest axial (14.1 mm) and axillary shoots (8.6 mm) were obtained on medium with 1.5 mg/l BA. Although both auxins proved to be efficient in rhizogenesis (rooting rates being 85.7% and 95.2%), higher values of all rooting parameters were observed in the presence of IBA. Rooted shoots were successfully acclimatized.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Proceedings of XIII International Scientific Agriculture Symposium „AGROSYM 2022“
T1  - Optimization of the protocol for in vitro propagation of autochthonous plum genotype ‘Metlaš’
EP  - 172
SP  - 166
ER  - 

@conference{
author = "Vujović, Tatjana and Jevremović, Darko and Anđelić, Tatjana and Vasilijević, Bojana",
year = "2022",
abstract = "Fast and cost-effective clonal propagation of planting material is possible to achieve by application of tissue culture in vitro. In this way, problems associated with traditional propagation, such as rapid spreading of diseases, lack of initial material caused by dependence on seasonal growth or low propagation coefficient can be overcome. This paper deals with optimization of micropropagation of autochthonous plum ‘Metlaš’ (Prunus domestica L.) originated from Guberevci (Municipality Knić, Serbia). ‘Metlaš’ is very often considered to be the same genotype as ‘Okruglica’ (syn. ‘Dragačica’), but they can be clearly distinguished according to plant habit, stone and fruit characteristics. To optimize multiplication stage, the influence of benzyladenine (BA at 0.5, 1.0, 1.5 and 2.0 mg/l) and thidiazuron (TDZ at 0.25, 0.5, 1.0 and 1.5 mg/l) on the multiplication capacity (multiplication index, length of axial and lateral shoots) was examined. Rooting ability of shoots (rooting rate, number and length of roots, and height of rooted plants) was monitored on half strength Murashige and Skoog (MS) medium containing 1.0 mg/l indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA), each combined with 0.1 mg/l gibberellic acid (GA3). TDZ applied at 0.25 mg/l gave the highest multiplication index (9.1), while the longest axial (14.1 mm) and axillary shoots (8.6 mm) were obtained on medium with 1.5 mg/l BA. Although both auxins proved to be efficient in rhizogenesis (rooting rates being 85.7% and 95.2%), higher values of all rooting parameters were observed in the presence of IBA. Rooted shoots were successfully acclimatized.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Proceedings of XIII International Scientific Agriculture Symposium „AGROSYM 2022“",
title = "Optimization of the protocol for in vitro propagation of autochthonous plum genotype ‘Metlaš’",
pages = "172-166"
}

Vujović, T., Jevremović, D., Anđelić, T.,& Vasilijević, B.. (2022). Optimization of the protocol for in vitro propagation of autochthonous plum genotype ‘Metlaš’. in Book of Proceedings of XIII International Scientific Agriculture Symposium „AGROSYM 2022“
East Sarajevo : Faculty of Agriculture., 166-172.

Vujović T, Jevremović D, Anđelić T, Vasilijević B. Optimization of the protocol for in vitro propagation of autochthonous plum genotype ‘Metlaš’. in Book of Proceedings of XIII International Scientific Agriculture Symposium „AGROSYM 2022“. 2022;:166-172..

Vujović, Tatjana, Jevremović, Darko, Anđelić, Tatjana, Vasilijević, Bojana, "Optimization of the protocol for in vitro propagation of autochthonous plum genotype ‘Metlaš’" in Book of Proceedings of XIII International Scientific Agriculture Symposium „AGROSYM 2022“ (2022):166-172.