TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Paunović, Svetlana A.
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/560
AB  - Little cherry virus 1 (LChV-1, genus Velarivirus) and little cherry virus 2 (LChV-2, genus
Ampelovirus), members of the family Closteroviridae, are considered important cherry pathogens
worldwide. In susceptible sweet and sour cherry cultivars, LChV-1 and LChV-2 can cause severe
yield losses and reduce fruit quality. Beside cherries, both viruses were found in other stone fruit
species: plums, apricots, peaches, and almonds. The aim of our study was to access the
presence of LChV-1 and LChV-2 in major Prunus species cultivated in Serbia.
During 2017−2020 202 stone fruit samples (67 sweet cherries, 17 sour cherries, 53 apricots, 38
plums, and 27 peaches) were collected from several regions of Serbia. All samples were analyzed
with reverse transcription polymerase chain reaction (RT-PCR). TNA was extracted from fresh
leaves with a 2% CTAB buffer. Reverse transcription was performed using random hexamer
primers. PCR reactions were performed using the LCV1U/LCV1L primer pair that amplify a 419 bp
fragment of the LChV-1 3' non-translated region and LCV2UP2/LCV2LO2 for the amplification of
the 438-bp fragment of the LChV-2 methyltransferase gene, respectively. PCR products were
analyzed by agarose gel electrophoresis. Selected isolates of both viruses (10 LChV-1, and one
LChV-2) were sequenced and phylogenetically analyzed.
The presence of LChV-1 was confirmed in 18 analyzed samples (8.9%), and LChV-2 in only one
sweet cherry sample (0.5%). LChV-1 was detected in 10 sweet cherries, five apricots, and three
plums. Analyzed viruses were not detected in any of the tested sour cherry and peach samples.
Serbian LChV-1 isolates showed 76.10–100% nucleotide (nt) identity. A phylogenetic analysis
identified Serbian LChV-1 isolates from plums and sweet cherries as members of the G5
phylogenetic group. Two apricot isolates belong to the G3 phylogenetic group and one isolate to
the G5 group.
This report describes the first report of little cherry virus 1 infecting plums in Serbia and confirms
the presence of isolates from G3 and G5 phylogroups in Serbian stone fruit orchards. Out of all
tested isolates, little cherry virus 2 was confirmed in only one sweet cherry tree.
PB  - Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health
C3  - Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
T1  - Occurrence of two little cherry viruses in stone fruits in Serbia
EP  - 78
SP  - 78
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Paunović, Svetlana A.",
year = "2023",
abstract = "Little cherry virus 1 (LChV-1, genus Velarivirus) and little cherry virus 2 (LChV-2, genusAmpelovirus), members of the family Closteroviridae, are considered important cherry pathogensworldwide. In susceptible sweet and sour cherry cultivars, LChV-1 and LChV-2 can cause severeyield losses and reduce fruit quality. Beside cherries, both viruses were found in other stone fruitspecies: plums, apricots, peaches, and almonds. The aim of our study was to access thepresence of LChV-1 and LChV-2 in major Prunus species cultivated in Serbia.During 2017−2020 202 stone fruit samples (67 sweet cherries, 17 sour cherries, 53 apricots, 38plums, and 27 peaches) were collected from several regions of Serbia. All samples were analyzedwith reverse transcription polymerase chain reaction (RT-PCR). TNA was extracted from freshleaves with a 2% CTAB buffer. Reverse transcription was performed using random hexamerprimers. PCR reactions were performed using the LCV1U/LCV1L primer pair that amplify a 419 bpfragment of the LChV-1 3' non-translated region and LCV2UP2/LCV2LO2 for the amplification ofthe 438-bp fragment of the LChV-2 methyltransferase gene, respectively. PCR products wereanalyzed by agarose gel electrophoresis. Selected isolates of both viruses (10 LChV-1, and oneLChV-2) were sequenced and phylogenetically analyzed.The presence of LChV-1 was confirmed in 18 analyzed samples (8.9%), and LChV-2 in only onesweet cherry sample (0.5%). LChV-1 was detected in 10 sweet cherries, five apricots, and threeplums. Analyzed viruses were not detected in any of the tested sour cherry and peach samples.Serbian LChV-1 isolates showed 76.10–100% nucleotide (nt) identity. A phylogenetic analysisidentified Serbian LChV-1 isolates from plums and sweet cherries as members of the G5phylogenetic group. Two apricot isolates belong to the G3 phylogenetic group and one isolate tothe G5 group.This report describes the first report of little cherry virus 1 infecting plums in Serbia and confirmsthe presence of isolates from G3 and G5 phylogroups in Serbian stone fruit orchards. Out of alltested isolates, little cherry virus 2 was confirmed in only one sweet cherry tree.",
publisher = "Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health",
journal = "Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023",
title = "Occurrence of two little cherry viruses in stone fruits in Serbia",
pages = "78-78"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Paunović, S. A.. (2023). Occurrence of two little cherry viruses in stone fruits in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health., 78-78.

Jevremović D, Vasilijević B, Katanić V, Paunović SA. Occurrence of two little cherry viruses in stone fruits in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023. 2023;:78-78..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Paunović, Svetlana A., "Occurrence of two little cherry viruses in stone fruits in Serbia" in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023 (2023):78-78.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Katanić, Vera
AU  - Paunović, Svetlana A.
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/559
AB  - Stone fruits (plums, apricots, peaches, and cherries) are economically important species for Serbian agriculture. These fruits harbor numerous viruses that can decrease yield and fruit quality. Cherry virus A (CVA), a member of the genus Capillovirus, was discovered during the cloning of the little cherry agent. CVA is widely distributed in more than 10 countries in sweet and sour cherries, but also in other Prunus hosts. So far, CVA has been confirmed in Serbia in sweet and sour cherries. A total of 106 leaf samples from 31 orchards in 22 locations were collected and examined to determine the prevalence of CVA in stone fruit orchards in Serbia. Great majority of leaf samples were symptomless, but some exhibited symptoms of chlorotic mottling, necrotic spots, and vein necrosis. Samples were tested by reverse transcription polymerase chain reaction (RT-PCR) using a virus-specific primer pair CVA-fw1/CVA-rev1 that amplify a fragment of the movement protein (MP) gene. PCR products were analyzed by electrophoresis in 1.5% agarose gel and stained with ethidium-bromide. To further access diversity of the detected isolates, we sequenced a partial MP of four detected isolates. The results of the RT-PCR revealed that 13 samples (12.3%) were infected with CVA. An expected 302 bp fragment was obtained in eight tested samples of sweet cherry, three apricot, and two plums. No positive amplification was evidenced in analyzed sour cherry and peach samples. CVA was detected in both asymptomatic and symptomatic samples. In all positive samples with symptoms, mixed infection with other viruses (prune dwarf virus, little cherry virus 1, and cherry green ring mottle virus) was detected. It is still unclear whether CVA is associated with any of the observed symptoms. The nucleotide sequences of the isolates showed 89.7–99.6% identity. To the best of our knowledge this is the first report of cherry virus A in apricots and plums in Serbia.
PB  - Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health
C3  - Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
T1  - Molecular detection and characterization of cherry virus a in Prunus species in Serbia
EP  - 79
SP  - 79
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Katanić, Vera and Paunović, Svetlana A.",
year = "2023",
abstract = "Stone fruits (plums, apricots, peaches, and cherries) are economically important species for Serbian agriculture. These fruits harbor numerous viruses that can decrease yield and fruit quality. Cherry virus A (CVA), a member of the genus Capillovirus, was discovered during the cloning of the little cherry agent. CVA is widely distributed in more than 10 countries in sweet and sour cherries, but also in other Prunus hosts. So far, CVA has been confirmed in Serbia in sweet and sour cherries. A total of 106 leaf samples from 31 orchards in 22 locations were collected and examined to determine the prevalence of CVA in stone fruit orchards in Serbia. Great majority of leaf samples were symptomless, but some exhibited symptoms of chlorotic mottling, necrotic spots, and vein necrosis. Samples were tested by reverse transcription polymerase chain reaction (RT-PCR) using a virus-specific primer pair CVA-fw1/CVA-rev1 that amplify a fragment of the movement protein (MP) gene. PCR products were analyzed by electrophoresis in 1.5% agarose gel and stained with ethidium-bromide. To further access diversity of the detected isolates, we sequenced a partial MP of four detected isolates. The results of the RT-PCR revealed that 13 samples (12.3%) were infected with CVA. An expected 302 bp fragment was obtained in eight tested samples of sweet cherry, three apricot, and two plums. No positive amplification was evidenced in analyzed sour cherry and peach samples. CVA was detected in both asymptomatic and symptomatic samples. In all positive samples with symptoms, mixed infection with other viruses (prune dwarf virus, little cherry virus 1, and cherry green ring mottle virus) was detected. It is still unclear whether CVA is associated with any of the observed symptoms. The nucleotide sequences of the isolates showed 89.7–99.6% identity. To the best of our knowledge this is the first report of cherry virus A in apricots and plums in Serbia.",
publisher = "Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health",
journal = "Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023",
title = "Molecular detection and characterization of cherry virus a in Prunus species in Serbia",
pages = "79-79"
}

Jevremović, D., Vasilijević, B., Katanić, V.,& Paunović, S. A.. (2023). Molecular detection and characterization of cherry virus a in Prunus species in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023
Wageningen : Netherlands Institute for Vectors, Invasive plants and Plant health., 79-79.

Jevremović D, Vasilijević B, Katanić V, Paunović SA. Molecular detection and characterization of cherry virus a in Prunus species in Serbia. in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023. 2023;:79-79..

Jevremović, Darko, Vasilijević, Bojana, Katanić, Vera, Paunović, Svetlana A., "Molecular detection and characterization of cherry virus a in Prunus species in Serbia" in Book of Аbstracts : The 25th International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops, Wageningen, Netherlands, July, 9-13  2023 (2023):79-79.

TY  - CHAP
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/543
PB  - USA : Elsevier Academic Press
T2  - Viral Diseases of Field and Horticultural Crops
T1  - Temperate fruits V Small fruits /soft fruits
EP  - 740
SP  - 733
ER  - 

@inbook{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2023",
publisher = "USA : Elsevier Academic Press",
journal = "Viral Diseases of Field and Horticultural Crops",
booktitle = "Temperate fruits V Small fruits /soft fruits",
pages = "740-733"
}

Jevremović, D.,& Paunović, S. A.. (2023). Temperate fruits V Small fruits /soft fruits. in Viral Diseases of Field and Horticultural Crops
USA : Elsevier Academic Press., 733-740.

Jevremović D, Paunović SA. Temperate fruits V Small fruits /soft fruits. in Viral Diseases of Field and Horticultural Crops. 2023;:733-740..

Jevremović, Darko, Paunović, Svetlana A., "Temperate fruits V Small fruits /soft fruits" in Viral Diseases of Field and Horticultural Crops (2023):733-740.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
AU  - Paunović, Svetlana A.
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/476
AB  - Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 12 viruses (Plum pox virus, Prune dwarf virus, Prunus necrotic ringspot virus, Apple chlorotic leafspot virus, Apple mosaic virus, Cherry leaf roll virus, Cherry necrotic rusty mottle virus, Cherry rasp leaf virus, Little cherry virus-1, Little cherry virus-2, Tomato black ring virus and Tomato ringspot virus) and Peach latent mosaic viroid. DAS-ELISA and molecular RT-PCR test were used for the detection with virus/viroid-specific primers. Analysis confirmed the presence of Plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of Peach latent mosaic viroid infection was 5,9%. No other viruses were detected among the collected samples. This study confirmed very low infection rate of viruses and viroids in tested peach samples.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Viruses and viroids infecting peaches in Serbia
EP  - 500
SP  - 495
VL  - 1352
DO  - 10.17660/ActaHortic.2022.1352.67
UR  - conv_1656
ER  - 

@conference{
author = "Jevremović, Darko and Vasilijević, Bojana and Paunović, Svetlana A.",
year = "2022",
abstract = "Peach and nectarine trees were surveyed from 2005 to 2021 to investigate the presence of virus and viroid diseases in Serbia. Samples were collected from commercial orchards in the main peach-growing areas and from nurseries and mother blocks. A total of 482 samples were tested on the presence of 12 viruses (Plum pox virus, Prune dwarf virus, Prunus necrotic ringspot virus, Apple chlorotic leafspot virus, Apple mosaic virus, Cherry leaf roll virus, Cherry necrotic rusty mottle virus, Cherry rasp leaf virus, Little cherry virus-1, Little cherry virus-2, Tomato black ring virus and Tomato ringspot virus) and Peach latent mosaic viroid. DAS-ELISA and molecular RT-PCR test were used for the detection with virus/viroid-specific primers. Analysis confirmed the presence of Plum pox virus in 16.2% of tested samples from commercial orchards. Further molecular characterization revealed the presence of PPV-M and PPV-D strains in positive samples. The incidence of Peach latent mosaic viroid infection was 5,9%. No other viruses were detected among the collected samples. This study confirmed very low infection rate of viruses and viroids in tested peach samples.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Viruses and viroids infecting peaches in Serbia",
pages = "500-495",
volume = "1352",
doi = "10.17660/ActaHortic.2022.1352.67",
url = "conv_1656"
}

Jevremović, D., Vasilijević, B.,& Paunović, S. A.. (2022). Viruses and viroids infecting peaches in Serbia. in Acta Horticulturae
International Society for Horticultural Science., 1352, 495-500.
https://doi.org/10.17660/ActaHortic.2022.1352.67
conv_1656

Jevremović D, Vasilijević B, Paunović SA. Viruses and viroids infecting peaches in Serbia. in Acta Horticulturae. 2022;1352:495-500.
doi:10.17660/ActaHortic.2022.1352.67
conv_1656 .

Jevremović, Darko, Vasilijević, Bojana, Paunović, Svetlana A., "Viruses and viroids infecting peaches in Serbia" in Acta Horticulturae, 1352 (2022):495-500,
https://doi.org/10.17660/ActaHortic.2022.1352.67 .,
conv_1656 .

TY  - CONF
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Živković, Sanja
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/777
AB  - Orah je rod biljaka iz porodice Juglandaceae. Na našim prostorima uglavnom se sreće vrsta Juglans regia L. Orah je samonikla biljka, dostiže visinu i do 25 metara. Do sada je rasprostranjenost oraha u Srbiji više bila posledica povoljnih prirodnih uslova nego organizovanog uticaja nauke. Orahovo drvo ima široku upotrebu u drvnoj industriji zbog svoje boje, dobre izdržljivosti i kvaliteta. Plod oraha ima široku upotrebu u prehrambenoj industriji. Lišće oraha može se koristiti za proizvodnju vina. Zelena opna ploda za proizvodnju likera – orahovače. Orahova smola je jestiva i puna eterničnih ulja. Lišće i kora oraha koriste se često kao laksativ. A pored toga koriste se i u tretmanima kože. Uzorci su sakupljani tokom jula 2019. godine u lokalitetu Dragobraća, Kragujevac.
Sakupljeni uzorci biljaka oraha su analizirani u fitopatološkoj laboratoriji Instituta za voćarstvo u Čačku. Iz zaraženih biljaka oraha (listova i grana), standardnim mikološkim postupcima, dobijeno je više izolata gljive, od kojih su za dalja proučavanja odabrana četiri izolata O1S, O2S poreklom sa grana i LO1, LO2 poreklom sa lista, koji su podvrgnuti testovima patogenosti korišćenjem dve različite metode, sa povređivanjem i bez povređivanja.
Morfološke odlike četiri odabrana izolata Alternaria spp. proučavane su na tri hranljive podloge: podloga od kukuruznog brašna (CMA), podloga od karanfila (CLA) i krompir dekstrozna podloga (PDA). Na KDA podlozi svi testirani izolati formirali su vazdušasto tamno maslinasto zelene
kolonije. Na podlozi od kukurunog brašna (CMA) kolonije su bile svetlo maslinasto zelene kod svih testiranih izolata. Na podlozi od karanfila (CLA) svi proučavani izolati formiraju supstratnu miceliju maslinasto zelene boje. Veličina konidija za sva četiri testirana izolatabila je 15,68-53,25 x 7,85-18,59 µm u proseku 36,01 x 14,20 µm. Svi prоučаvаni izоlаti nа tеstirаnim biljnim delovima (list i plod) oraha izаzvаli su simptоmе tipičnе zа glјivе iz rоdа Alternaria, tо јеst pојаvu tаmnо brаоn nеkrоtičnih lеziја nа listovima i plodovima. Na osnovu preliminarnih istraživanja patogenih i morfoloških karakteristika utvrđeno je da ispitivani izolati O1S, O2S poreklom sa grana i LO1, LO2 poreklom sa lista pripadaju gljivama iz roda Alternaria. U toku je molekularna identifikacija i karakterizacija ispitivanih izolata.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, 22-25. februar 2021, Zlatibor, Republika Srbija
T1  - Morfološke i patogene karakteristike izolata Alternaria spp. na orahu
EP  - 22
SP  - 21
ER  - 

@conference{
author = "Vasić, Tanja and Jevremović, Darko and Živković, Sanja and Leposavić, Aleksandar and Paunović, Svetlana A.",
year = "2021",
abstract = "Orah je rod biljaka iz porodice Juglandaceae. Na našim prostorima uglavnom se sreće vrsta Juglans regia L. Orah je samonikla biljka, dostiže visinu i do 25 metara. Do sada je rasprostranjenost oraha u Srbiji više bila posledica povoljnih prirodnih uslova nego organizovanog uticaja nauke. Orahovo drvo ima široku upotrebu u drvnoj industriji zbog svoje boje, dobre izdržljivosti i kvaliteta. Plod oraha ima široku upotrebu u prehrambenoj industriji. Lišće oraha može se koristiti za proizvodnju vina. Zelena opna ploda za proizvodnju likera – orahovače. Orahova smola je jestiva i puna eterničnih ulja. Lišće i kora oraha koriste se često kao laksativ. A pored toga koriste se i u tretmanima kože. Uzorci su sakupljani tokom jula 2019. godine u lokalitetu Dragobraća, Kragujevac.Sakupljeni uzorci biljaka oraha su analizirani u fitopatološkoj laboratoriji Instituta za voćarstvo u Čačku. Iz zaraženih biljaka oraha (listova i grana), standardnim mikološkim postupcima, dobijeno je više izolata gljive, od kojih su za dalja proučavanja odabrana četiri izolata O1S, O2S poreklom sa grana i LO1, LO2 poreklom sa lista, koji su podvrgnuti testovima patogenosti korišćenjem dve različite metode, sa povređivanjem i bez povređivanja.Morfološke odlike četiri odabrana izolata Alternaria spp. proučavane su na tri hranljive podloge: podloga od kukuruznog brašna (CMA), podloga od karanfila (CLA) i krompir dekstrozna podloga (PDA). Na KDA podlozi svi testirani izolati formirali su vazdušasto tamno maslinasto zelenekolonije. Na podlozi od kukurunog brašna (CMA) kolonije su bile svetlo maslinasto zelene kod svih testiranih izolata. Na podlozi od karanfila (CLA) svi proučavani izolati formiraju supstratnu miceliju maslinasto zelene boje. Veličina konidija za sva četiri testirana izolatabila je 15,68-53,25 x 7,85-18,59 µm u proseku 36,01 x 14,20 µm. Svi prоučаvаni izоlаti nа tеstirаnim biljnim delovima (list i plod) oraha izаzvаli su simptоmе tipičnе zа glјivе iz rоdа Alternaria, tо јеst pојаvu tаmnо brаоn nеkrоtičnih lеziја nа listovima i plodovima. Na osnovu preliminarnih istraživanja patogenih i morfoloških karakteristika utvrđeno je da ispitivani izolati O1S, O2S poreklom sa grana i LO1, LO2 poreklom sa lista pripadaju gljivama iz roda Alternaria. U toku je molekularna identifikacija i karakterizacija ispitivanih izolata.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, 22-25. februar 2021, Zlatibor, Republika Srbija",
title = "Morfološke i patogene karakteristike izolata Alternaria spp. na orahu",
pages = "22-21"
}

Vasić, T., Jevremović, D., Živković, S., Leposavić, A.,& Paunović, S. A.. (2021). Morfološke i patogene karakteristike izolata Alternaria spp. na orahu. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, 22-25. februar 2021, Zlatibor, Republika Srbija
Beograd: Društvo za zaštitu bilja Srbije., 21-22.

Vasić T, Jevremović D, Živković S, Leposavić A, Paunović SA. Morfološke i patogene karakteristike izolata Alternaria spp. na orahu. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, 22-25. februar 2021, Zlatibor, Republika Srbija. 2021;:21-22..

Vasić, Tanja, Jevremović, Darko, Živković, Sanja, Leposavić, Aleksandar, Paunović, Svetlana A., "Morfološke i patogene karakteristike izolata Alternaria spp. na orahu" in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, 22-25. februar 2021, Zlatibor, Republika Srbija (2021):21-22.

TY  - CONF
AU  - Jevremović, Darko
AU  - Vujović, Tatjana
AU  - Milošević, Nebojša
AU  - Paunović, Svetlana A.
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/435
AB  - The conservation of genetic diversity and its sustainable use are essential to meet a number of challenges facing humanity. European plum (Prunus domestica L.), as the leading fruit species in Serbian agriculture, is facing with climate changes and long-term inoculum pressure of numerous pathogens. In order to identify the trees as a source for cryopreservation using vitrification-based techniques (V and D cryo-plate) we collected samples from nine autochthonous cultivars. A total of 140 samples of 'Belosljiva', 'Crvena Ranka', 'Crnosljiva', 'Cerovacki Piskavac', 'Dragacevka', 'Moravka', 'Požegaca', 'Sitnica' and 'Trnovaca' plums were collected from various geographical locations in Serbia that are considered as their origin. Samples were collected from symptomless trees showing typical pomological characteristics of the cultivars (true-to-type). All samples were tested for the presence of Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Plum bark necrosis stem pitting-associated virus (PBNSPaV), Myrobalan latent ringspot virus (MLRSV) and 'Candidatus Phytoplasma prunorum'. The analysis for the virus presence was performed by ELISA and RT-PCR test. Samples were additionally tested for PPV presence by Real-time PCR, whereas 'Candidatus Phytoplasma prunorum' was detected by nested-PCR. Of all analysed pathogens, only PPV was detected in 6 samples. The presented results showed that, even in the high virus inoculum pressure, it is possible to select virus-free trees from existing old orchards. After analysis, selected healthy trees were used as a source of explants for the establishment of aseptic culture of each genotype for the conservation purpose.
PB  - Leuven : International Society for Horticultural Science
C3  - Proceedings : XII International Symposium on Plum and Prune Genetics, Breeding and Pomology,  September 14–17, 2021, Zlatibor, Republic of Serbia, Acta Horticulturae
T1  - Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes
EP  - 81
SP  - 77
VL  - 1322
DO  - 10.17660/ActaHortic.2021.1322.12
UR  - conv_1660
ER  - 

@conference{
author = "Jevremović, Darko and Vujović, Tatjana and Milošević, Nebojša and Paunović, Svetlana A.",
year = "2021",
abstract = "The conservation of genetic diversity and its sustainable use are essential to meet a number of challenges facing humanity. European plum (Prunus domestica L.), as the leading fruit species in Serbian agriculture, is facing with climate changes and long-term inoculum pressure of numerous pathogens. In order to identify the trees as a source for cryopreservation using vitrification-based techniques (V and D cryo-plate) we collected samples from nine autochthonous cultivars. A total of 140 samples of 'Belosljiva', 'Crvena Ranka', 'Crnosljiva', 'Cerovacki Piskavac', 'Dragacevka', 'Moravka', 'Požegaca', 'Sitnica' and 'Trnovaca' plums were collected from various geographical locations in Serbia that are considered as their origin. Samples were collected from symptomless trees showing typical pomological characteristics of the cultivars (true-to-type). All samples were tested for the presence of Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Plum bark necrosis stem pitting-associated virus (PBNSPaV), Myrobalan latent ringspot virus (MLRSV) and 'Candidatus Phytoplasma prunorum'. The analysis for the virus presence was performed by ELISA and RT-PCR test. Samples were additionally tested for PPV presence by Real-time PCR, whereas 'Candidatus Phytoplasma prunorum' was detected by nested-PCR. Of all analysed pathogens, only PPV was detected in 6 samples. The presented results showed that, even in the high virus inoculum pressure, it is possible to select virus-free trees from existing old orchards. After analysis, selected healthy trees were used as a source of explants for the establishment of aseptic culture of each genotype for the conservation purpose.",
publisher = "Leuven : International Society for Horticultural Science",
journal = "Proceedings : XII International Symposium on Plum and Prune Genetics, Breeding and Pomology,  September 14–17, 2021, Zlatibor, Republic of Serbia, Acta Horticulturae",
title = "Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes",
pages = "81-77",
volume = "1322",
doi = "10.17660/ActaHortic.2021.1322.12",
url = "conv_1660"
}

Jevremović, D., Vujović, T., Milošević, N.,& Paunović, S. A.. (2021). Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes. in Proceedings : XII International Symposium on Plum and Prune Genetics, Breeding and Pomology,  September 14–17, 2021, Zlatibor, Republic of Serbia, Acta Horticulturae
Leuven : International Society for Horticultural Science., 1322, 77-81.
https://doi.org/10.17660/ActaHortic.2021.1322.12
conv_1660

Jevremović D, Vujović T, Milošević N, Paunović SA. Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes. in Proceedings : XII International Symposium on Plum and Prune Genetics, Breeding and Pomology,  September 14–17, 2021, Zlatibor, Republic of Serbia, Acta Horticulturae. 2021;1322:77-81.
doi:10.17660/ActaHortic.2021.1322.12
conv_1660 .

Jevremović, Darko, Vujović, Tatjana, Milošević, Nebojša, Paunović, Svetlana A., "Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes" in Proceedings : XII International Symposium on Plum and Prune Genetics, Breeding and Pomology,  September 14–17, 2021, Zlatibor, Republic of Serbia, Acta Horticulturae, 1322 (2021):77-81,
https://doi.org/10.17660/ActaHortic.2021.1322.12 .,
conv_1660 .

TY  - CONF
AU  - Paunović, Svetlana A.
AU  - Glišić, Ivana
AU  - Karaklajić-Stajić, Žaklina
AU  - Milošević, Nebojša
AU  - Jevremović, Darko
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/437
AB  - 'Lana' was recognized as a new plum cultivar developed at the Fruit Research Institute, Cacak in 2020. It is the 18th plum cultivar created at the Institute. 'Lana' was developed by planned hybridization of 'California Blue' × 'Ruth Gershtetter' and aimed selection of obtained plum hybrids of early fruit ripening time, good fruit quality, positive pomological properties, regular and optimal yield, and resistance or tolerance to the main plum pathogens. The new cultivar is characterized by a moderately vigorous to vigorous tree, a rare canopy with narrow-pyramidal shape. The branches are well garnished with fruiting wood. Flowering time is medium early similar to standard plum cultivar 'Cacanska Rana' with which it was evaluated in parallel. It is a self-incompatible cultivar. The ripening season is early, occurring in the second decade of July, about 4-10 days after 'Cacanska Rana'. It crops regularly and moderately. In field conditions, under natural pathogen infections pressure, 'Lana' shows no susceptibility to the Polistigma rubrum (Pers.) DC, Puccinia pruni-spinosae (Pers.: Pers.) and Taphrina pruni (Fuck.) Tul, while sporadic symptoms of brown rot can be observed pointing at moderate susceptibility to Monilinia laxa (Aderhold & Ruhland) Honey. 'Lana' is tolerant to Sharka, it could exhibit sporadic symptoms on individual leaves but not on the fruits. The fruit is large, with average weight of 86.34 g, roundish, with very pronounced suture line. The skin is purple-blue with mildly expressed bloom. The flesh is yellow-greenish, moderately firm, juicy and aromatic with sweet-subacidic flavor, having 97.08% of share in the fruit weight. It contains an average of 14.5% soluble solids, 9.95% total sugar and 1.34% total acids. The stone is large and partially separated from the flesh. 'Lana' is a plum cultivar intended primarily for fresh consumption. It is recommended for growing in suitable agro-ecological conditions of vineyard areas.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Performance of 'Lana', a new plum cultivar developed at Fruit Research Institute, Cacak
EP  - 32
SP  - 25
VL  - 1322
DO  - 10.17660/ActaHortic.2021.1322.5
UR  - conv_1662
ER  - 

@conference{
author = "Paunović, Svetlana A. and Glišić, Ivana and Karaklajić-Stajić, Žaklina and Milošević, Nebojša and Jevremović, Darko",
year = "2021",
abstract = "'Lana' was recognized as a new plum cultivar developed at the Fruit Research Institute, Cacak in 2020. It is the 18th plum cultivar created at the Institute. 'Lana' was developed by planned hybridization of 'California Blue' × 'Ruth Gershtetter' and aimed selection of obtained plum hybrids of early fruit ripening time, good fruit quality, positive pomological properties, regular and optimal yield, and resistance or tolerance to the main plum pathogens. The new cultivar is characterized by a moderately vigorous to vigorous tree, a rare canopy with narrow-pyramidal shape. The branches are well garnished with fruiting wood. Flowering time is medium early similar to standard plum cultivar 'Cacanska Rana' with which it was evaluated in parallel. It is a self-incompatible cultivar. The ripening season is early, occurring in the second decade of July, about 4-10 days after 'Cacanska Rana'. It crops regularly and moderately. In field conditions, under natural pathogen infections pressure, 'Lana' shows no susceptibility to the Polistigma rubrum (Pers.) DC, Puccinia pruni-spinosae (Pers.: Pers.) and Taphrina pruni (Fuck.) Tul, while sporadic symptoms of brown rot can be observed pointing at moderate susceptibility to Monilinia laxa (Aderhold & Ruhland) Honey. 'Lana' is tolerant to Sharka, it could exhibit sporadic symptoms on individual leaves but not on the fruits. The fruit is large, with average weight of 86.34 g, roundish, with very pronounced suture line. The skin is purple-blue with mildly expressed bloom. The flesh is yellow-greenish, moderately firm, juicy and aromatic with sweet-subacidic flavor, having 97.08% of share in the fruit weight. It contains an average of 14.5% soluble solids, 9.95% total sugar and 1.34% total acids. The stone is large and partially separated from the flesh. 'Lana' is a plum cultivar intended primarily for fresh consumption. It is recommended for growing in suitable agro-ecological conditions of vineyard areas.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Performance of 'Lana', a new plum cultivar developed at Fruit Research Institute, Cacak",
pages = "32-25",
volume = "1322",
doi = "10.17660/ActaHortic.2021.1322.5",
url = "conv_1662"
}

Paunović, S. A., Glišić, I., Karaklajić-Stajić, Ž., Milošević, N.,& Jevremović, D.. (2021). Performance of 'Lana', a new plum cultivar developed at Fruit Research Institute, Cacak. in Acta Horticulturae
International Society for Horticultural Science., 1322, 25-32.
https://doi.org/10.17660/ActaHortic.2021.1322.5
conv_1662

Paunović SA, Glišić I, Karaklajić-Stajić Ž, Milošević N, Jevremović D. Performance of 'Lana', a new plum cultivar developed at Fruit Research Institute, Cacak. in Acta Horticulturae. 2021;1322:25-32.
doi:10.17660/ActaHortic.2021.1322.5
conv_1662 .

Paunović, Svetlana A., Glišić, Ivana, Karaklajić-Stajić, Žaklina, Milošević, Nebojša, Jevremović, Darko, "Performance of 'Lana', a new plum cultivar developed at Fruit Research Institute, Cacak" in Acta Horticulturae, 1322 (2021):25-32,
https://doi.org/10.17660/ActaHortic.2021.1322.5 .,
conv_1662 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/467
AB  - This study describes molecular detection, high prevalence and geographical distribution of blueberry latent virus (BlLV) in Serbia. BlLV was discovered in the USA and the main signature of the virus is latent infection. A number of 110 samples from 14 highbush blueberry cultivars collected from different locations in 9 municipalities of Serbia were tested by RT-PCR with BlLV-specific primers. The presence of BlLV was confirmed in 69 samples (62.7%) in all surveyed locations. BlLV was confirmed in single infections, but also in a mixed infection with blueberry mosaic-associated virus and blueberry red ringspot virus. To access the genetic diversity, direct sequencing of the fragment from the fusion protein gene of 14 Serbian isolates was done. Serbian isolates showed 99.64-100% nucleotide (nt) and 98.94-100% amino acid (aa) identity. In comparison with the available BlLV sequences of the isolates from Bosnia and Herzegovina, Japan, South Korea and the USA, our isolates showed 99.29-100 nt and 97.87-100% aa similarity.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Plant Diseases and Protection
T1  - Molecular detection, geographical distribution and genetic diversity of blueberry latent virus in highbush blueberries in Serbia
EP  - 605
IS  - 2
SP  - 601
VL  - 128
DO  - 10.1007/s41348-020-00418-7
UR  - conv_1462
ER  - 

@article{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2021",
abstract = "This study describes molecular detection, high prevalence and geographical distribution of blueberry latent virus (BlLV) in Serbia. BlLV was discovered in the USA and the main signature of the virus is latent infection. A number of 110 samples from 14 highbush blueberry cultivars collected from different locations in 9 municipalities of Serbia were tested by RT-PCR with BlLV-specific primers. The presence of BlLV was confirmed in 69 samples (62.7%) in all surveyed locations. BlLV was confirmed in single infections, but also in a mixed infection with blueberry mosaic-associated virus and blueberry red ringspot virus. To access the genetic diversity, direct sequencing of the fragment from the fusion protein gene of 14 Serbian isolates was done. Serbian isolates showed 99.64-100% nucleotide (nt) and 98.94-100% amino acid (aa) identity. In comparison with the available BlLV sequences of the isolates from Bosnia and Herzegovina, Japan, South Korea and the USA, our isolates showed 99.29-100 nt and 97.87-100% aa similarity.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Plant Diseases and Protection",
title = "Molecular detection, geographical distribution and genetic diversity of blueberry latent virus in highbush blueberries in Serbia",
pages = "605-601",
number = "2",
volume = "128",
doi = "10.1007/s41348-020-00418-7",
url = "conv_1462"
}

Jevremović, D.,& Paunović, S. A.. (2021). Molecular detection, geographical distribution and genetic diversity of blueberry latent virus in highbush blueberries in Serbia. in Journal of Plant Diseases and Protection
Springer Heidelberg, Heidelberg., 128(2), 601-605.
https://doi.org/10.1007/s41348-020-00418-7
conv_1462

Jevremović D, Paunović SA. Molecular detection, geographical distribution and genetic diversity of blueberry latent virus in highbush blueberries in Serbia. in Journal of Plant Diseases and Protection. 2021;128(2):601-605.
doi:10.1007/s41348-020-00418-7
conv_1462 .

Jevremović, Darko, Paunović, Svetlana A., "Molecular detection, geographical distribution and genetic diversity of blueberry latent virus in highbush blueberries in Serbia" in Journal of Plant Diseases and Protection, 128, no. 2 (2021):601-605,
https://doi.org/10.1007/s41348-020-00418-7 .,
conv_1462 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/466
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - First report of raspberry latent virus in Europe
EP  - 1013
IS  - 3
SP  - 1013
VL  - 103
DO  - 10.1007/s42161-021-00833-1
UR  - conv_1612
ER  - 

@article{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2021",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "First report of raspberry latent virus in Europe",
pages = "1013-1013",
number = "3",
volume = "103",
doi = "10.1007/s42161-021-00833-1",
url = "conv_1612"
}

Jevremović, D.,& Paunović, S. A.. (2021). First report of raspberry latent virus in Europe. in Journal of Plant Pathology
Springer, New York., 103(3), 1013-1013.
https://doi.org/10.1007/s42161-021-00833-1
conv_1612

Jevremović D, Paunović SA. First report of raspberry latent virus in Europe. in Journal of Plant Pathology. 2021;103(3):1013-1013.
doi:10.1007/s42161-021-00833-1
conv_1612 .

Jevremović, Darko, Paunović, Svetlana A., "First report of raspberry latent virus in Europe" in Journal of Plant Pathology, 103, no. 3 (2021):1013-1013,
https://doi.org/10.1007/s42161-021-00833-1 .,
conv_1612 .

TY  - CONF
AU  - Glišić, Ivana
AU  - Paunović, Svetlana A.
AU  - Milatović, Dragan
AU  - Jevremović, Darko
AU  - Milošević, Nebojša
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/455
AB  - Plum breeding at the Fruit Research Institute, Cacak, Republic of Serbia, has a decades-long tradition and significant results achieved. So far, 15 new plum cultivars have been recognized. Positive selection of promising hybrids obtained in continuous breeding programmes is based not only on biological, pomological and productive properties but also on their resistance or tolerance to the causal agents of economically the most important diseases. Reaction of promising plum hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87, and the cultivar 'Nada' to Plum pox virus - PPV and causal agents of red leaf spot [Polystigma rubrum (Pers.) DC], rust [Puccinia prunispinosae (Pers.: Pers.)] and blossom blight and brown rot [Monilinia laxa (Aderhold & Ruhland) Honey] were evaluated in the field under natural high infection pressure condition during a three-year period (2009-2011). Resistance to PPV was also examined by artificial inoculation with characterized PPV-M, PPV-D and PPV-Rec isolates in controlled conditions. The hybrids 32/21/87, 34/41/87 and 22/17/87 exhibited high levels of resistance to natural infections by PPV and causal agents of red leaf spot and rust. No typical symptoms of blossom blight and brown rot were found on the hybrids IV/63/81 and 32/21/87. The hybrids 32/21/87, 34/41/87 and 22/17/87 showed also resistance or high degree of tolerance to infection with certain PPV strains by artificial inoculation.
PB  - International Society for Horticultural Science, Leuven 1
C3  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future
T1  - Evaluation of promising plum (Prunus domestica L.) genotypes for resistance to causal agents of the most important diseases
EP  - 332
SP  - 325
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.46
UR  - conv_1630
ER  - 

@conference{
author = "Glišić, Ivana and Paunović, Svetlana A. and Milatović, Dragan and Jevremović, Darko and Milošević, Nebojša",
year = "2021",
abstract = "Plum breeding at the Fruit Research Institute, Cacak, Republic of Serbia, has a decades-long tradition and significant results achieved. So far, 15 new plum cultivars have been recognized. Positive selection of promising hybrids obtained in continuous breeding programmes is based not only on biological, pomological and productive properties but also on their resistance or tolerance to the causal agents of economically the most important diseases. Reaction of promising plum hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87, and the cultivar 'Nada' to Plum pox virus - PPV and causal agents of red leaf spot [Polystigma rubrum (Pers.) DC], rust [Puccinia prunispinosae (Pers.: Pers.)] and blossom blight and brown rot [Monilinia laxa (Aderhold & Ruhland) Honey] were evaluated in the field under natural high infection pressure condition during a three-year period (2009-2011). Resistance to PPV was also examined by artificial inoculation with characterized PPV-M, PPV-D and PPV-Rec isolates in controlled conditions. The hybrids 32/21/87, 34/41/87 and 22/17/87 exhibited high levels of resistance to natural infections by PPV and causal agents of red leaf spot and rust. No typical symptoms of blossom blight and brown rot were found on the hybrids IV/63/81 and 32/21/87. The hybrids 32/21/87, 34/41/87 and 22/17/87 showed also resistance or high degree of tolerance to infection with certain PPV strains by artificial inoculation.",
publisher = "International Society for Horticultural Science, Leuven 1",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future",
title = "Evaluation of promising plum (Prunus domestica L.) genotypes for resistance to causal agents of the most important diseases",
pages = "332-325",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.46",
url = "conv_1630"
}

Glišić, I., Paunović, S. A., Milatović, D., Jevremović, D.,& Milošević, N.. (2021). Evaluation of promising plum (Prunus domestica L.) genotypes for resistance to causal agents of the most important diseases. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future
International Society for Horticultural Science, Leuven 1., 1308, 325-332.
https://doi.org/10.17660/ActaHortic.2021.1308.46
conv_1630

Glišić I, Paunović SA, Milatović D, Jevremović D, Milošević N. Evaluation of promising plum (Prunus domestica L.) genotypes for resistance to causal agents of the most important diseases. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future. 2021;1308:325-332.
doi:10.17660/ActaHortic.2021.1308.46
conv_1630 .

Glišić, Ivana, Paunović, Svetlana A., Milatović, Dragan, Jevremović, Darko, Milošević, Nebojša, "Evaluation of promising plum (Prunus domestica L.) genotypes for resistance to causal agents of the most important diseases" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future, 1308 (2021):325-332,
https://doi.org/10.17660/ActaHortic.2021.1308.46 .,
conv_1630 .

TY  - CONF
AU  - Krstić, Branka
AU  - Paunović, Svetlana A.
AU  - Jasnić, Stevan
AU  - Bagi, Ferenc
AU  - Mijatović, Mirjana
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
AU  - Delibašić, Goran
AU  - Milošević, Drago
AU  - Jevremović, Darko
AU  - Milošević, Dragana
AU  - Stanković, Ivana
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/566
AB  - Neprestani demografski rast sveta i potreba da se stanovništvo snabde prirodnim
proizvodima i hranom kontrolisanog kvaliteta, nametnuli su potrebu proučavanja virusnih
bolesti biljaka. Osim praktičnog značaja u razvijanju uspešnih strategija u suzbijanju virusa i
sprečavanju ili umanjenju šteta izazvanih virusima, biljna virusologija ima i veliki
fundamentalni značaj u otkrivanju bioloških, epidemioloških i evolutivnih zakonitosti i pojava.
Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck
proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum
fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su
naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub
Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja
viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj
virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu
(otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja
i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava
virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa,
mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije
virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim
međuodnosima). Baveći se različitim oblastima biljne virusologije u proteklih gotovo osam
decenija, brojni virusolozi ostvarili su značajna dostignuća. Posebne zasluge za razvoj biljne
virusologije svakako pripadaju Dragoljubu Šutiću, koji je zahvaljujući svom širokim
obrazovanju, uspešnom eksperimetalnom radu, dobro razvijenoj domaćoj i međunarodnoj
saradnji, ostvarenim rezultatima i brojnim naučnim radovima i publikacijama, značajno
doprineo obrazovanju mnogih generacija studenata, i dao nemerljiv doprinos unapređenju zdravlja bilja. Razvoj biljne virusologije u našoj zemlji nije zavisio samo od opšteg napretka
ove nauke u svetu, već u velikoj meri i od uslova za razvoj naučnih istraživanja. I pored
relativno skromnih uslova koji vladaju na naučno-istraživačkoj sceni virusologije, rad biljnih
virusologa u proteklom periodu podigao je kvalitet istraživanja trudeći se da dostigne i ne
zaostaje za kvalitetom razvijenih zemalja. Značajno iskustvo u decenijskom radu u oblasti
razvojnih i primenjenih istraživanja u biljnoj virusologiji i sva stečena saznanja o biljnim
virusima bila su, i sada su od neprocenjivog značaja za poljoprivredne proizvođače i
unapređenje njihove proizvodnje, za unapređenje sistema i efikasnosti karantinske i
fitosanitarne službe, rad ovlašćenih dijagnostičkih laboratorija, ispunjavanje međunarodnih
fitosanitarnih zahteva u prometu i razmeni biljnog materijala, i podizanju ugleda Srbije.
Većina publikacija je rezultat istraživanja etiologije, simptomatologije, epidemiologije,
genetičke strukture populacije, otpornosti prema virusima i drugih mera kontrole sa ciljem
iznalaženja rešenja od praktičnog značaja.
Iako je razvoj biljne virusologije u Srbiji pokrio mnoge fundamentalne i praktične
aspekte ove nauke, očekuju se nastavak istraživanja zasnovanih na mogućnostima koja nam
pružaju druge nauke, pre svega molekularna biologija i bioinformatika. Zalog za sigurnu
budućnost virusologije u našoj zemlji je obrazovanje i osposobljavanje mladih istraživača.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021
T1  - Istorijski razvoj biljne virusologije u Srbiji
EP  - 20
SP  - 19
ER  - 

@conference{
author = "Krstić, Branka and Paunović, Svetlana A. and Jasnić, Stevan and Bagi, Ferenc and Mijatović, Mirjana and Starović, Mira and Kuzmanović, Slobodan and Delibašić, Goran and Milošević, Drago and Jevremović, Darko and Milošević, Dragana and Stanković, Ivana",
year = "2021",
abstract = "Neprestani demografski rast sveta i potreba da se stanovništvo snabde prirodnimproizvodima i hranom kontrolisanog kvaliteta, nametnuli su potrebu proučavanja virusnihbolesti biljaka. Osim praktičnog značaja u razvijanju uspešnih strategija u suzbijanju virusa isprečavanju ili umanjenju šteta izazvanih virusima, biljna virusologija ima i velikifundamentalni značaj u otkrivanju bioloških, epidemioloških i evolutivnih zakonitosti i pojava.Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinckproučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivumfluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela sunaučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, DragoljubŠutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanjaviroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvojvirusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu(otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanjai širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastavavirusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa,mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacijevirusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnimmeđuodnosima). Baveći se različitim oblastima biljne virusologije u proteklih gotovo osamdecenija, brojni virusolozi ostvarili su značajna dostignuća. Posebne zasluge za razvoj biljnevirusologije svakako pripadaju Dragoljubu Šutiću, koji je zahvaljujući svom širokimobrazovanju, uspešnom eksperimetalnom radu, dobro razvijenoj domaćoj i međunarodnojsaradnji, ostvarenim rezultatima i brojnim naučnim radovima i publikacijama, značajnodoprineo obrazovanju mnogih generacija studenata, i dao nemerljiv doprinos unapređenju zdravlja bilja. Razvoj biljne virusologije u našoj zemlji nije zavisio samo od opšteg napretkaove nauke u svetu, već u velikoj meri i od uslova za razvoj naučnih istraživanja. I poredrelativno skromnih uslova koji vladaju na naučno-istraživačkoj sceni virusologije, rad biljnihvirusologa u proteklom periodu podigao je kvalitet istraživanja trudeći se da dostigne i nezaostaje za kvalitetom razvijenih zemalja. Značajno iskustvo u decenijskom radu u oblastirazvojnih i primenjenih istraživanja u biljnoj virusologiji i sva stečena saznanja o biljnimvirusima bila su, i sada su od neprocenjivog značaja za poljoprivredne proizvođače iunapređenje njihove proizvodnje, za unapređenje sistema i efikasnosti karantinske ifitosanitarne službe, rad ovlašćenih dijagnostičkih laboratorija, ispunjavanje međunarodnihfitosanitarnih zahteva u prometu i razmeni biljnog materijala, i podizanju ugleda Srbije.Većina publikacija je rezultat istraživanja etiologije, simptomatologije, epidemiologije,genetičke strukture populacije, otpornosti prema virusima i drugih mera kontrole sa ciljemiznalaženja rešenja od praktičnog značaja.Iako je razvoj biljne virusologije u Srbiji pokrio mnoge fundamentalne i praktičneaspekte ove nauke, očekuju se nastavak istraživanja zasnovanih na mogućnostima koja nampružaju druge nauke, pre svega molekularna biologija i bioinformatika. Zalog za sigurnubudućnost virusologije u našoj zemlji je obrazovanje i osposobljavanje mladih istraživača.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021",
title = "Istorijski razvoj biljne virusologije u Srbiji",
pages = "20-19"
}

Krstić, B., Paunović, S. A., Jasnić, S., Bagi, F., Mijatović, M., Starović, M., Kuzmanović, S., Delibašić, G., Milošević, D., Jevremović, D., Milošević, D.,& Stanković, I.. (2021). Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021
Beograd: Društvo za zaštitu bilja Srbije., 19-20.

Krstić B, Paunović SA, Jasnić S, Bagi F, Mijatović M, Starović M, Kuzmanović S, Delibašić G, Milošević D, Jevremović D, Milošević D, Stanković I. Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 2021;:19-20..

Krstić, Branka, Paunović, Svetlana A., Jasnić, Stevan, Bagi, Ferenc, Mijatović, Mirjana, Starović, Mira, Kuzmanović, Slobodan, Delibašić, Goran, Milošević, Drago, Jevremović, Darko, Milošević, Dragana, Stanković, Ivana, "Istorijski razvoj biljne virusologije u Srbiji" in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021 (2021):19-20.

TY  - CONF
AU  - Vasić, Tanja
AU  - Jevremović, Darko
AU  - Živković, Sanja
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/565
AB  - Poslednjih decenija američka visokožbunasta borovnica (Vaccinium corymbosum L.)
dobija sve značajnije mesto u voćarstvu više zemalja sveta, a posebno u visokorazvijenim.
Takođe, sve je veći interes proizvođača jagodastog voća iz Srbije za podizanje zasada
visokožbunaste borovnice. Trenutno se u Srbiji visokožbunasta borovnica gaji na površini od
oko 2.500 ha sa tendencijom rasta proizvodnje. Borovnicu napadaju brojne bolesti koje mogu
prouzrokovati veće ekonomske štete, a u izuzetnim slučajevima dovesti do propadanja čitavih
zasada.
Sistematskih istraživanja mikopopulacije borovnice u Srbiji do sada nije bilo. U ovome radu
iznosimo rezultate preliminarnih istraživanja mikopopulacije borovnice poreklom iz Srbije.
Uzorci su prikupljeni u periodu jun-avgust 2020. godine. Ukupno je analizirano 18 uzoraka sa
pet lokaliteta (Ivanjica 3, Čačak 3, Sopot 3, Bačka Topla 6 i Zlatibor 3). Delovi korenovog
varata i grana su pažljivo isprani pod tekućom vodom. Tako pripremljeni uzorci dezinfikovani
su sa 1% natrijum-hipokloritom (NaOCl) 1 min. i isprani tri puta u sterilnoj destilovanoj vodi.
Potom su prosušeni na sterilnom filter papiru i stavljani na krompir dekstrozni agar (KDA). U
svaku Petri kutiju stavljeno je po 5 biljnih delova, u pet ponavljanja. Držani su u termostatu
na 24°C . Pregled se obavljao svaka 3 dana, a na većini uzoraka micelija se razvila do 14. dana.
Razvijene micelije presejane su na novu KDA podlogu te je, nakon početnog porasta, vršni dio
micelija ponovno presejan na KDA. Mikroskopski pregled obavljen je pomoću mikroskopa
marke Olympus CX31. Morfološka identifikacija gljiva do roda obavljena je pomoću
standardnih ključeva.
Ukupno je pregledano 350 biljnih delova sa kojih je izolovano 8 rodova gljiva:
Fusarium, Phomopsis (Diaporthe), Alternaria, Epicoccum, Penicillium, Ulocladium, Trichoderma
i Pestalotiopsis. Na biljkama iz kojih su izolovane gljive bili su jasno vidljivi makroskopski
simptomi zaraze.
S оbzirоm nа sve veći znаčај borovnice kao jagodaste voćne vrste, cilј оvоg rаdа је
dеtеrminаciја glјivа, potencijalnih prоuzrоkоvаčа bоlеsti nа borovnici, rаdi јаsniјеg
sаglеdаvаnjа prоblеmа (izumirаnjе bilјаkа, smаnjеnjе prinоsа i drugо) kојi nаstајu kао
pоslеdicа prisustvа glјivа nа borovnici.
PB  - Beograd: Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021
T1  - Mikopopulacija na američkoj visokožbunastoj borovnici u Srbiji
EP  - 41
SP  - 41
ER  - 

@conference{
author = "Vasić, Tanja and Jevremović, Darko and Živković, Sanja and Leposavić, Aleksandar and Paunović, Svetlana A.",
year = "2021",
abstract = "Poslednjih decenija američka visokožbunasta borovnica (Vaccinium corymbosum L.)dobija sve značajnije mesto u voćarstvu više zemalja sveta, a posebno u visokorazvijenim.Takođe, sve je veći interes proizvođača jagodastog voća iz Srbije za podizanje zasadavisokožbunaste borovnice. Trenutno se u Srbiji visokožbunasta borovnica gaji na površini odoko 2.500 ha sa tendencijom rasta proizvodnje. Borovnicu napadaju brojne bolesti koje moguprouzrokovati veće ekonomske štete, a u izuzetnim slučajevima dovesti do propadanja čitavihzasada.Sistematskih istraživanja mikopopulacije borovnice u Srbiji do sada nije bilo. U ovome raduiznosimo rezultate preliminarnih istraživanja mikopopulacije borovnice poreklom iz Srbije.Uzorci su prikupljeni u periodu jun-avgust 2020. godine. Ukupno je analizirano 18 uzoraka sapet lokaliteta (Ivanjica 3, Čačak 3, Sopot 3, Bačka Topla 6 i Zlatibor 3). Delovi korenovogvarata i grana su pažljivo isprani pod tekućom vodom. Tako pripremljeni uzorci dezinfikovanisu sa 1% natrijum-hipokloritom (NaOCl) 1 min. i isprani tri puta u sterilnoj destilovanoj vodi.Potom su prosušeni na sterilnom filter papiru i stavljani na krompir dekstrozni agar (KDA). Usvaku Petri kutiju stavljeno je po 5 biljnih delova, u pet ponavljanja. Držani su u termostatuna 24°C . Pregled se obavljao svaka 3 dana, a na većini uzoraka micelija se razvila do 14. dana.Razvijene micelije presejane su na novu KDA podlogu te je, nakon početnog porasta, vršni diomicelija ponovno presejan na KDA. Mikroskopski pregled obavljen je pomoću mikroskopamarke Olympus CX31. Morfološka identifikacija gljiva do roda obavljena je pomoćustandardnih ključeva.Ukupno je pregledano 350 biljnih delova sa kojih je izolovano 8 rodova gljiva:Fusarium, Phomopsis (Diaporthe), Alternaria, Epicoccum, Penicillium, Ulocladium, Trichodermai Pestalotiopsis. Na biljkama iz kojih su izolovane gljive bili su jasno vidljivi makroskopskisimptomi zaraze.S оbzirоm nа sve veći znаčај borovnice kao jagodaste voćne vrste, cilј оvоg rаdа јеdеtеrminаciја glјivа, potencijalnih prоuzrоkоvаčа bоlеsti nа borovnici, rаdi јаsniјеgsаglеdаvаnjа prоblеmа (izumirаnjе bilјаkа, smаnjеnjе prinоsа i drugо) kојi nаstајu kаоpоslеdicа prisustvа glјivа nа borovnici.",
publisher = "Beograd: Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021",
title = "Mikopopulacija na američkoj visokožbunastoj borovnici u Srbiji",
pages = "41-41"
}

Vasić, T., Jevremović, D., Živković, S., Leposavić, A.,& Paunović, S. A.. (2021). Mikopopulacija na američkoj visokožbunastoj borovnici u Srbiji. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021
Beograd: Društvo za zaštitu bilja Srbije., 41-41.

Vasić T, Jevremović D, Živković S, Leposavić A, Paunović SA. Mikopopulacija na američkoj visokožbunastoj borovnici u Srbiji. in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 2021;:41-41..

Vasić, Tanja, Jevremović, Darko, Živković, Sanja, Leposavić, Aleksandar, Paunović, Svetlana A., "Mikopopulacija na američkoj visokožbunastoj borovnici u Srbiji" in Zbornik rezimea radova : XVI savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021 (2021):41-41.

TY  - CONF
AU  - Jevremović, Darko
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/417
AB  - Black raspberry necrosis virus (BRNV) is considered to be economically important virus infecting raspberries. In black raspberry (Rubus occidentalis L.) BRNV causes cane tip necrosis, but infected red raspberry (Rubus idaeus L.) cultivars in general do not express foliar symptoms. From 2014–2018, a number of 105 samples from 9 red raspberry cultivars (‘Amira’, ‘Fertödi Zamatos’, ‘Glen Ample’, ‘Meeker’, ‘San Rafael’, ‘Polana’, ‘Polka’, ‘Tulameen’ and ‘Willamette’) were collected from different localities in Serbia. Samples were tested on the BRNV presence by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers targeting 417 bp nucleotide (nt) fragment of the RNA-dependent RNA polymerase (RdRp) region of RNA 1 of the BRNV genome. BRNV was detected in 19 samples in cultivars ‘Fertödi Zamatos’, ‘Polana’, ‘Tulameen’ and ‘Willamette’. A 417 nt sequence of the RdRp was determined for 5 isolates. The nt sequences of these isolates were 94.2–99.7% identical. When compared to available sequences of BRNV isolates from North America and Europe, Serbian isolates showed significant divergence (80.0–82.2% of nt identity). A portion of the BRNV infected plants of cultivar ‘Fertödi Zamatos’ were further tested on indicator R. occidentalis by inarch bottle grafting. Two months after inoculation cane tip begun to curl down and became necrotic on all inoculated plants. Non-grafted control plants were symptomless.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Molecular and biological characterization of black raspberry necrosis virus on red raspberry in Serbia
EP  - 87
SP  - 82
VL  - 78
DO  - 10.1007/978-3-030-40049-1_10
UR  - conv_1671
ER  - 

@conference{
author = "Jevremović, Darko and Leposavić, Aleksandar and Paunović, Svetlana A.",
year = "2020",
abstract = "Black raspberry necrosis virus (BRNV) is considered to be economically important virus infecting raspberries. In black raspberry (Rubus occidentalis L.) BRNV causes cane tip necrosis, but infected red raspberry (Rubus idaeus L.) cultivars in general do not express foliar symptoms. From 2014–2018, a number of 105 samples from 9 red raspberry cultivars (‘Amira’, ‘Fertödi Zamatos’, ‘Glen Ample’, ‘Meeker’, ‘San Rafael’, ‘Polana’, ‘Polka’, ‘Tulameen’ and ‘Willamette’) were collected from different localities in Serbia. Samples were tested on the BRNV presence by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers targeting 417 bp nucleotide (nt) fragment of the RNA-dependent RNA polymerase (RdRp) region of RNA 1 of the BRNV genome. BRNV was detected in 19 samples in cultivars ‘Fertödi Zamatos’, ‘Polana’, ‘Tulameen’ and ‘Willamette’. A 417 nt sequence of the RdRp was determined for 5 isolates. The nt sequences of these isolates were 94.2–99.7% identical. When compared to available sequences of BRNV isolates from North America and Europe, Serbian isolates showed significant divergence (80.0–82.2% of nt identity). A portion of the BRNV infected plants of cultivar ‘Fertödi Zamatos’ were further tested on indicator R. occidentalis by inarch bottle grafting. Two months after inoculation cane tip begun to curl down and became necrotic on all inoculated plants. Non-grafted control plants were symptomless.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Molecular and biological characterization of black raspberry necrosis virus on red raspberry in Serbia",
pages = "87-82",
volume = "78",
doi = "10.1007/978-3-030-40049-1_10",
url = "conv_1671"
}

Jevremović, D., Leposavić, A.,& Paunović, S. A.. (2020). Molecular and biological characterization of black raspberry necrosis virus on red raspberry in Serbia. in IFMBE Proceedings
Springer., 78, 82-87.
https://doi.org/10.1007/978-3-030-40049-1_10
conv_1671

Jevremović D, Leposavić A, Paunović SA. Molecular and biological characterization of black raspberry necrosis virus on red raspberry in Serbia. in IFMBE Proceedings. 2020;78:82-87.
doi:10.1007/978-3-030-40049-1_10
conv_1671 .

Jevremović, Darko, Leposavić, Aleksandar, Paunović, Svetlana A., "Molecular and biological characterization of black raspberry necrosis virus on red raspberry in Serbia" in IFMBE Proceedings, 78 (2020):82-87,
https://doi.org/10.1007/978-3-030-40049-1_10 .,
conv_1671 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
AU  - Leposavić, Aleksandar
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/521
AB  - Blueberry mosaic has been described several decades ago, but the viral nature of the disease was confirmed recently. The causal agent of the disease is blueberry mosaic associated virus (BlMaV), genus Ophiovirus. BlMaV induce leaf mottling and bright yellow, green, and pink to red mosaic patterns in infected bushes. Symptoms usually appear on several leaves or cover the entire bush, but the infection may be latent for several years. BlMaV was confirmed in two locations in Serbia in varieties ‘Duke’, ‘Bluecrop’ and ‘Goldtraub’. The aim of this study was to evaluate the influence of BlMaV on some fruit traits of highbush blueberry ‘Duke’. The trial was conducted in 2019 in locality Budoželja, West Serbia in the orchard with the confirmed BlMaV presence. Based on the results of RT-PCR analysis, three groups of blueberry ‘Duke’ bushes were selected: BlMaV-free, BlMaV infected – mosaic symptoms present on only several leaves, and BlMaV infected – mosaic symptoms on entire bush. The influence of BlMaV on pomological variables (fruit length, width and height, shape, fruit weight, and soluble solids content) was examined. The results showed that BlMaV did not have statistically significant impact on any of the examined fruit traits. The evaluation of virus influence on the composition of nutritive and bioactive compounds is underway.
AB  - Мозаечният вирус по боровинката е описан преди няколко десетилетия, но вирусната природа на заболяването е потвърдена наскоро. Причинителят на заболяването е мозаечният вирус по боровинката (BlMaV), род Ophiovirus. BlMaV причинява листни петна и ярко жълти, зелени и розови до червени мозаечни шарки в заразените храсти. Симптомите обикновено се появяват на няколко листа или покриват целия храст, но инфекцията може да е латентна в продължение на няколко години. BlMaV е потвърден на две места в Сърбия при сортове „Дюк“, „Блукроб“ и „Голдтрауб“. Целта на настоящото проучване е да се оцени влиянието на BlMaV върху някои плодови характеристики на висококачествена боровинка „Дюк“. Опитът е проведен през 2019 г. В местност Будожела, Западна Сърбия в овощна градина с потвърденото присъствие на BlMaV. Въз основа на резултатите от RT-PCR анализа са избрани три групи от боровинки „Дюк“: Без зараза BlMaV, BlMaV инфектирани – мозаечни симптоми присъстват само на няколко листа, и BlMaV инфектирани - мозаечни симптоми върху целия храст. Изследвано е влиянието на BlMaV върху помологични променливи (дължина, ширина и височина на плодовете, форма, тегло на плодовете и съдържание на разтворимисухи вещества). Резултатите показват, че BlMaV не оказва статистически значимо въздействие върху нито една от изследваните плодови характеристики. В момента се извършва оценка на влиянието на вируса върху състава на хранителни и биоактивни съединения.
PB  - Troyan : Research Institute of Mountain Stockbreeding and Agriculture
T2  - Journal of Mountain Agriculture on the Balkans
T1  - Influence of blueberry mosaic associated virus on some fruit traits of highbush blueberry ‘Duke’
T1  - Влияние на мозаечния вирус върху някои характеристики на плодовете на високохрастовидна боровинка сорт "Дюк"
EP  - 203
IS  - 3
SP  - 195
VL  - 23
ER  - 

@article{
author = "Jevremović, Darko and Paunović, Svetlana A. and Leposavić, Aleksandar",
year = "2020",
abstract = "Blueberry mosaic has been described several decades ago, but the viral nature of the disease was confirmed recently. The causal agent of the disease is blueberry mosaic associated virus (BlMaV), genus Ophiovirus. BlMaV induce leaf mottling and bright yellow, green, and pink to red mosaic patterns in infected bushes. Symptoms usually appear on several leaves or cover the entire bush, but the infection may be latent for several years. BlMaV was confirmed in two locations in Serbia in varieties ‘Duke’, ‘Bluecrop’ and ‘Goldtraub’. The aim of this study was to evaluate the influence of BlMaV on some fruit traits of highbush blueberry ‘Duke’. The trial was conducted in 2019 in locality Budoželja, West Serbia in the orchard with the confirmed BlMaV presence. Based on the results of RT-PCR analysis, three groups of blueberry ‘Duke’ bushes were selected: BlMaV-free, BlMaV infected – mosaic symptoms present on only several leaves, and BlMaV infected – mosaic symptoms on entire bush. The influence of BlMaV on pomological variables (fruit length, width and height, shape, fruit weight, and soluble solids content) was examined. The results showed that BlMaV did not have statistically significant impact on any of the examined fruit traits. The evaluation of virus influence on the composition of nutritive and bioactive compounds is underway., Мозаечният вирус по боровинката е описан преди няколко десетилетия, но вирусната природа на заболяването е потвърдена наскоро. Причинителят на заболяването е мозаечният вирус по боровинката (BlMaV), род Ophiovirus. BlMaV причинява листни петна и ярко жълти, зелени и розови до червени мозаечни шарки в заразените храсти. Симптомите обикновено се появяват на няколко листа или покриват целия храст, но инфекцията може да е латентна в продължение на няколко години. BlMaV е потвърден на две места в Сърбия при сортове „Дюк“, „Блукроб“ и „Голдтрауб“. Целта на настоящото проучване е да се оцени влиянието на BlMaV върху някои плодови характеристики на висококачествена боровинка „Дюк“. Опитът е проведен през 2019 г. В местност Будожела, Западна Сърбия в овощна градина с потвърденото присъствие на BlMaV. Въз основа на резултатите от RT-PCR анализа са избрани три групи от боровинки „Дюк“: Без зараза BlMaV, BlMaV инфектирани – мозаечни симптоми присъстват само на няколко листа, и BlMaV инфектирани - мозаечни симптоми върху целия храст. Изследвано е влиянието на BlMaV върху помологични променливи (дължина, ширина и височина на плодовете, форма, тегло на плодовете и съдържание на разтворимисухи вещества). Резултатите показват, че BlMaV не оказва статистически значимо въздействие върху нито една от изследваните плодови характеристики. В момента се извършва оценка на влиянието на вируса върху състава на хранителни и биоактивни съединения.",
publisher = "Troyan : Research Institute of Mountain Stockbreeding and Agriculture",
journal = "Journal of Mountain Agriculture on the Balkans",
title = "Influence of blueberry mosaic associated virus on some fruit traits of highbush blueberry ‘Duke’, Влияние на мозаечния вирус върху някои характеристики на плодовете на високохрастовидна боровинка сорт 'Дюк'",
pages = "203-195",
number = "3",
volume = "23"
}

Jevremović, D., Paunović, S. A.,& Leposavić, A.. (2020). Influence of blueberry mosaic associated virus on some fruit traits of highbush blueberry ‘Duke’. in Journal of Mountain Agriculture on the Balkans
Troyan : Research Institute of Mountain Stockbreeding and Agriculture., 23(3), 195-203.

Jevremović D, Paunović SA, Leposavić A. Influence of blueberry mosaic associated virus on some fruit traits of highbush blueberry ‘Duke’. in Journal of Mountain Agriculture on the Balkans. 2020;23(3):195-203..

Jevremović, Darko, Paunović, Svetlana A., Leposavić, Aleksandar, "Influence of blueberry mosaic associated virus on some fruit traits of highbush blueberry ‘Duke’" in Journal of Mountain Agriculture on the Balkans, 23, no. 3 (2020):195-203.

TY  - JOUR
AU  - Paunović, Svetlana A.
AU  - Jevremović, Darko
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/431
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - The first detection of raspberry leaf mottle virus in Serbia
EP  - 925
IS  - 3
SP  - 925
VL  - 102
DO  - 10.1007/s42161-020-00507-4
UR  - conv_1601
ER  - 

@article{
author = "Paunović, Svetlana A. and Jevremović, Darko",
year = "2020",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "The first detection of raspberry leaf mottle virus in Serbia",
pages = "925-925",
number = "3",
volume = "102",
doi = "10.1007/s42161-020-00507-4",
url = "conv_1601"
}

Paunović, S. A.,& Jevremović, D.. (2020). The first detection of raspberry leaf mottle virus in Serbia. in Journal of Plant Pathology
Springer, New York., 102(3), 925-925.
https://doi.org/10.1007/s42161-020-00507-4
conv_1601

Paunović SA, Jevremović D. The first detection of raspberry leaf mottle virus in Serbia. in Journal of Plant Pathology. 2020;102(3):925-925.
doi:10.1007/s42161-020-00507-4
conv_1601 .

Paunović, Svetlana A., Jevremović, Darko, "The first detection of raspberry leaf mottle virus in Serbia" in Journal of Plant Pathology, 102, no. 3 (2020):925-925,
https://doi.org/10.1007/s42161-020-00507-4 .,
conv_1601 .

TY  - CONF
AU  - Miletić, Nemanja
AU  - Jevremović, Darko
AU  - Mitrović, Olga
AU  - Gvozdenović, O.
AU  - Pajić, M.
AU  - Paunović, Svetlana A.
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/425
AB  - Plum pox virus (PPV) is economically the most important virus of stone fruits. In sensitive plum (Prunus domestica L.) cultivars, PPV causes foliar symptoms, fruits deformation, changes in the chemical composition of fruits and premature fruit drop. Fruits of ‘Čačanska lepotica’ plum cultivar were collected from an experimental orchard in locality Čačak. Samples were collected from 15 trees (5 infected with PPV-D, 5 infected with PPV-Rec and 5 from PPV-free trees). Trees for sampling were selected after the RT-PCR analysis of the trees from the plantation using PPV strain-specific primers. The fruits were picked at five different terms at seven-days interval during the entire maturation stage. All tested samples, 10 PPV-infected and 5 uninfected, were further chemically analyzed. The analyses included determination of soluble solid content, titratable acidity and total phenolic content. The aim of this work was to reveal the possible changes in chemical composition in the fruits of ‘Čačanska lepotica’ cultivar, grown at the same locality, caused by the two different PPV strains.
PB  - Springer
C3  - IFMBE Proceedings
T1  - Influence of different plum pox virus strains on chemical composition of 'Čačanska lepotica' plum fruit cultivar
EP  - 81
SP  - 76
VL  - 78
DO  - 10.1007/978-3-030-40049-1_9
UR  - conv_1672
ER  - 

@conference{
author = "Miletić, Nemanja and Jevremović, Darko and Mitrović, Olga and Gvozdenović, O. and Pajić, M. and Paunović, Svetlana A.",
year = "2020",
abstract = "Plum pox virus (PPV) is economically the most important virus of stone fruits. In sensitive plum (Prunus domestica L.) cultivars, PPV causes foliar symptoms, fruits deformation, changes in the chemical composition of fruits and premature fruit drop. Fruits of ‘Čačanska lepotica’ plum cultivar were collected from an experimental orchard in locality Čačak. Samples were collected from 15 trees (5 infected with PPV-D, 5 infected with PPV-Rec and 5 from PPV-free trees). Trees for sampling were selected after the RT-PCR analysis of the trees from the plantation using PPV strain-specific primers. The fruits were picked at five different terms at seven-days interval during the entire maturation stage. All tested samples, 10 PPV-infected and 5 uninfected, were further chemically analyzed. The analyses included determination of soluble solid content, titratable acidity and total phenolic content. The aim of this work was to reveal the possible changes in chemical composition in the fruits of ‘Čačanska lepotica’ cultivar, grown at the same locality, caused by the two different PPV strains.",
publisher = "Springer",
journal = "IFMBE Proceedings",
title = "Influence of different plum pox virus strains on chemical composition of 'Čačanska lepotica' plum fruit cultivar",
pages = "81-76",
volume = "78",
doi = "10.1007/978-3-030-40049-1_9",
url = "conv_1672"
}

Miletić, N., Jevremović, D., Mitrović, O., Gvozdenović, O., Pajić, M.,& Paunović, S. A.. (2020). Influence of different plum pox virus strains on chemical composition of 'Čačanska lepotica' plum fruit cultivar. in IFMBE Proceedings
Springer., 78, 76-81.
https://doi.org/10.1007/978-3-030-40049-1_9
conv_1672

Miletić N, Jevremović D, Mitrović O, Gvozdenović O, Pajić M, Paunović SA. Influence of different plum pox virus strains on chemical composition of 'Čačanska lepotica' plum fruit cultivar. in IFMBE Proceedings. 2020;78:76-81.
doi:10.1007/978-3-030-40049-1_9
conv_1672 .

Miletić, Nemanja, Jevremović, Darko, Mitrović, Olga, Gvozdenović, O., Pajić, M., Paunović, Svetlana A., "Influence of different plum pox virus strains on chemical composition of 'Čačanska lepotica' plum fruit cultivar" in IFMBE Proceedings, 78 (2020):76-81,
https://doi.org/10.1007/978-3-030-40049-1_9 .,
conv_1672 .

TY  - JOUR
AU  - Paunović, Svetlana A.
AU  - Jevremović, Darko
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/423
AB  - Uzorci crvene maline (Rubus idaeus L.) iz najvažnijih područja gajenja u Srbiji su analizirani na prisustvo četiri najvažnija virusa maline, virusa mrljavosti lista maline (RLBV), virusa nekroze crne maline (BRNV), virusa žute mrežavosti Rubusa (RYNV) i virusa šarenila lista maline (RLMV), njihovu pojedinačnu, kao i zastupljenost u mešanim infekcijama. Testirana su 74 uzorka osam sorti maline, odabrana sa 32 najvažnija lokaliteta gajenja, od kojih su 62 uzorka listova bili sa simptomima koji bi mogli biti izazvani virusima, a 12 uzoraka bez simptoma. Prisustvo virusa je testirano primenom odgovarajuće metode, RT-PCR i PCR, korišćenjem virus specifi čnih prajmera. Detektovano je prisustvo sva četiri virusa u zasadima u Srbiji sa visoko značajnim razlikama u zastupljenosti u ukupno šezdeset jednom zaraženom uzorku sa simptomima. Zaraze sa jednim od četiri ispitivana virusa su detektovane u 59,0% svih zaraženih uzoraka. Najzastupljeni je bio RLBV koji je povezan sa sindromom lisne mrljavosti maline u združenom prisustvu sa svojim vektorom, grinjom lista i pupoljka maline P. gracillis, detektovan u 70,5% zaraženih uzoraka. Slede RYNV sa učestalošću od 42,6%, BRNV sa 36,1% i RLMV sa zastupljenošću od 9,8% svih zaraženih uzoraka. Dobijeni rezultati su po prvi put u Srbiji pokazali značajnu zastupljenost (41,0%) mešanih infekcija sa dva do četiri virusa. Najzastupljenije su infekcije sa dva virusa, 68,0% svih mešano zaraženih uzoraka, a među njima mešane infekcijie RLBV i RYNV (44,0%). Virusi iz kompleksa mozaika maline, BRNV, RYNV i RLMV, uključujući i mešane-infekcije 2-3 navedena virusa sa RLBV, su utvrđeni u visokoj združenoj zastupljenosti od 14,7% svih zaraženih uzoraka, odnosno 36,0% uzoraka sa mešanim infekcijama.
AB  - Samples of red raspberry (Rubus idaeus L.) from the most important growing areas in Serbia were analyzed for the presence of four most important raspberry viruses: raspberry leaf blotch virus (RLBV), raspberry leaf mottle virus (RLMV), black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and their incidence in individual and mixed infected samples. Seventy-four samples of eight raspberry cultivars, selected from 32 main cultivation sites were tested, of which 62 leaf samples showed symptoms that could be caused by viruses and 12 samples were asymptomatic. The presence of viruses was tested by appropriate RT-PCR and PCR methods, using virus-specific primers. All four viruses were detected with highly significant differences of incidence in a total of 61 infected symptomatic samples. Infection with one of the four tested viruses was detected in 59.0% of all infected samples. The most prevalent was RLBV, which was associated with raspberry leaf blotch disorder in coexistence with its vector raspberry leaf and bud mite P. gracillis, and it was detected in 70.5% of the infected samples. It was followed by RYNV with an incidence of 42.6%, BRNV with 36.1% and RLMV with 9.8% incidence, considering all infected samples. The obtained results for the first time in Serbia showed a high proportion (41.0%) of mixed infections involving between two and four viruses. The most common were infections with two viruses, 68.0% of all mixed-infected samples, and among them coinfections with RLBV and RYNV (44.0%). Viruses of the raspberry mosaic complex, BRNV, RYNV and RLMV, including coinfections of 2-3 listed viruses along with RLBV, were found in a high joined proportion of 14.7% of all infected samples, i.e. 36.0% of mixed infected samples.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Visoka zastupljenost mešanih virusnih infekcija u zasadima maline u Srbiji
T1  - High proportion of mixed virus infections in raspberry plantations in Serbia
EP  - 37
IS  - 1
SP  - 27
VL  - 35
DO  - 10.2298/PIF2001027P
UR  - conv_1301
ER  - 

@article{
author = "Paunović, Svetlana A. and Jevremović, Darko",
year = "2020",
abstract = "Uzorci crvene maline (Rubus idaeus L.) iz najvažnijih područja gajenja u Srbiji su analizirani na prisustvo četiri najvažnija virusa maline, virusa mrljavosti lista maline (RLBV), virusa nekroze crne maline (BRNV), virusa žute mrežavosti Rubusa (RYNV) i virusa šarenila lista maline (RLMV), njihovu pojedinačnu, kao i zastupljenost u mešanim infekcijama. Testirana su 74 uzorka osam sorti maline, odabrana sa 32 najvažnija lokaliteta gajenja, od kojih su 62 uzorka listova bili sa simptomima koji bi mogli biti izazvani virusima, a 12 uzoraka bez simptoma. Prisustvo virusa je testirano primenom odgovarajuće metode, RT-PCR i PCR, korišćenjem virus specifi čnih prajmera. Detektovano je prisustvo sva četiri virusa u zasadima u Srbiji sa visoko značajnim razlikama u zastupljenosti u ukupno šezdeset jednom zaraženom uzorku sa simptomima. Zaraze sa jednim od četiri ispitivana virusa su detektovane u 59,0% svih zaraženih uzoraka. Najzastupljeni je bio RLBV koji je povezan sa sindromom lisne mrljavosti maline u združenom prisustvu sa svojim vektorom, grinjom lista i pupoljka maline P. gracillis, detektovan u 70,5% zaraženih uzoraka. Slede RYNV sa učestalošću od 42,6%, BRNV sa 36,1% i RLMV sa zastupljenošću od 9,8% svih zaraženih uzoraka. Dobijeni rezultati su po prvi put u Srbiji pokazali značajnu zastupljenost (41,0%) mešanih infekcija sa dva do četiri virusa. Najzastupljenije su infekcije sa dva virusa, 68,0% svih mešano zaraženih uzoraka, a među njima mešane infekcijie RLBV i RYNV (44,0%). Virusi iz kompleksa mozaika maline, BRNV, RYNV i RLMV, uključujući i mešane-infekcije 2-3 navedena virusa sa RLBV, su utvrđeni u visokoj združenoj zastupljenosti od 14,7% svih zaraženih uzoraka, odnosno 36,0% uzoraka sa mešanim infekcijama., Samples of red raspberry (Rubus idaeus L.) from the most important growing areas in Serbia were analyzed for the presence of four most important raspberry viruses: raspberry leaf blotch virus (RLBV), raspberry leaf mottle virus (RLMV), black raspberry necrosis virus (BRNV) and Rubus yellow net virus (RYNV), and their incidence in individual and mixed infected samples. Seventy-four samples of eight raspberry cultivars, selected from 32 main cultivation sites were tested, of which 62 leaf samples showed symptoms that could be caused by viruses and 12 samples were asymptomatic. The presence of viruses was tested by appropriate RT-PCR and PCR methods, using virus-specific primers. All four viruses were detected with highly significant differences of incidence in a total of 61 infected symptomatic samples. Infection with one of the four tested viruses was detected in 59.0% of all infected samples. The most prevalent was RLBV, which was associated with raspberry leaf blotch disorder in coexistence with its vector raspberry leaf and bud mite P. gracillis, and it was detected in 70.5% of the infected samples. It was followed by RYNV with an incidence of 42.6%, BRNV with 36.1% and RLMV with 9.8% incidence, considering all infected samples. The obtained results for the first time in Serbia showed a high proportion (41.0%) of mixed infections involving between two and four viruses. The most common were infections with two viruses, 68.0% of all mixed-infected samples, and among them coinfections with RLBV and RYNV (44.0%). Viruses of the raspberry mosaic complex, BRNV, RYNV and RLMV, including coinfections of 2-3 listed viruses along with RLBV, were found in a high joined proportion of 14.7% of all infected samples, i.e. 36.0% of mixed infected samples.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Visoka zastupljenost mešanih virusnih infekcija u zasadima maline u Srbiji, High proportion of mixed virus infections in raspberry plantations in Serbia",
pages = "37-27",
number = "1",
volume = "35",
doi = "10.2298/PIF2001027P",
url = "conv_1301"
}

Paunović, S. A.,& Jevremović, D.. (2020). Visoka zastupljenost mešanih virusnih infekcija u zasadima maline u Srbiji. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 35(1), 27-37.
https://doi.org/10.2298/PIF2001027P
conv_1301

Paunović SA, Jevremović D. Visoka zastupljenost mešanih virusnih infekcija u zasadima maline u Srbiji. in Pesticidi i fitomedicina. 2020;35(1):27-37.
doi:10.2298/PIF2001027P
conv_1301 .

Paunović, Svetlana A., Jevremović, Darko, "Visoka zastupljenost mešanih virusnih infekcija u zasadima maline u Srbiji" in Pesticidi i fitomedicina, 35, no. 1 (2020):27-37,
https://doi.org/10.2298/PIF2001027P .,
conv_1301 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/522
AB  - Plum pox virus (PPV, genus Potyvirus) is the causal agent of Sharka disease that is considered the most detrimental viral disease of stone fruits. Since its discovery in 1932 in Bulgaria, the disease has spread progressively to entire Europe and other continents, except Australia. PPV causes significant yield losses and reduces fruit quality in sensitive plum, peach and apricot cultivars. Sharka was reported in Serbia in 1935, and so far the presence of three major strains (PPV-M, -D and -Rec) was confirmed. Fifteen years ago, a largescale study on the presence, distribution, genetic diversity and epidemiology of Plum pox virus strains has begun at the Fruit Research Institute, Čačak. In this review, we summarized the results of our field and laboratory research and presented the future prospects for disease control in the country where Sharka is endemic to maintain feasible commercial fruit production.
AB  - Plum pox virus (PPV, род Potyvirus) е причинител на болестта шарка, която се счита за най-пагубното вирусно заболяване при костилковите видове. От своето откритие през 1932 г. в България болестта се разпространява прогресивно в цяла Европа и други континенти, с изключение на Австралия. PPV причинява значителни загуби в добива и понижава качеството на плодовете при чувствителни сортове сливи, праскови и кайсии. Шарката е установена в Сърбия през 1935 г. И досега е потвърдено наличието на три основни щама (PPV-M, -D и -Rec). Преди петнадесет години в Изследователски институт по овощарство, Чачак, започна широко мащабно проучване за наличието, разпространението, генетичното разнообразие и епидемиологията на щамовете на Plum pox virus. В настоящoто изследване са обобщени резултатите от наши полски и лабораторни изследвания, предста вени са бъдещи перспективи за контрол върху болестта в Сърбия, където шарка та е ендемична, за приемливо поддържане търговско производство на плодове.
PB  - Troyan : Research Institute of Mountain Stockbreeding and Agriculture
T2  - Journal of Mountain Agriculture on the Balkans
T1  - Sharka (plum pox virus) in Serbia – previous research and future prospects for Its control
T1  - Болестта шарка (plum pox virus) в Сърбия – предишни изследвания и бъдещи перспективи за контрол
EP  - 176
IS  - 2
SP  - 162
VL  - 22
ER  - 

@article{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2019",
abstract = "Plum pox virus (PPV, genus Potyvirus) is the causal agent of Sharka disease that is considered the most detrimental viral disease of stone fruits. Since its discovery in 1932 in Bulgaria, the disease has spread progressively to entire Europe and other continents, except Australia. PPV causes significant yield losses and reduces fruit quality in sensitive plum, peach and apricot cultivars. Sharka was reported in Serbia in 1935, and so far the presence of three major strains (PPV-M, -D and -Rec) was confirmed. Fifteen years ago, a largescale study on the presence, distribution, genetic diversity and epidemiology of Plum pox virus strains has begun at the Fruit Research Institute, Čačak. In this review, we summarized the results of our field and laboratory research and presented the future prospects for disease control in the country where Sharka is endemic to maintain feasible commercial fruit production., Plum pox virus (PPV, род Potyvirus) е причинител на болестта шарка, която се счита за най-пагубното вирусно заболяване при костилковите видове. От своето откритие през 1932 г. в България болестта се разпространява прогресивно в цяла Европа и други континенти, с изключение на Австралия. PPV причинява значителни загуби в добива и понижава качеството на плодовете при чувствителни сортове сливи, праскови и кайсии. Шарката е установена в Сърбия през 1935 г. И досега е потвърдено наличието на три основни щама (PPV-M, -D и -Rec). Преди петнадесет години в Изследователски институт по овощарство, Чачак, започна широко мащабно проучване за наличието, разпространението, генетичното разнообразие и епидемиологията на щамовете на Plum pox virus. В настоящoто изследване са обобщени резултатите от наши полски и лабораторни изследвания, предста вени са бъдещи перспективи за контрол върху болестта в Сърбия, където шарка та е ендемична, за приемливо поддържане търговско производство на плодове.",
publisher = "Troyan : Research Institute of Mountain Stockbreeding and Agriculture",
journal = "Journal of Mountain Agriculture on the Balkans",
title = "Sharka (plum pox virus) in Serbia – previous research and future prospects for Its control, Болестта шарка (plum pox virus) в Сърбия – предишни изследвания и бъдещи перспективи за контрол",
pages = "176-162",
number = "2",
volume = "22"
}

Jevremović, D.,& Paunović, S. A.. (2019). Sharka (plum pox virus) in Serbia – previous research and future prospects for Its control. in Journal of Mountain Agriculture on the Balkans
Troyan : Research Institute of Mountain Stockbreeding and Agriculture., 22(2), 162-176.

Jevremović D, Paunović SA. Sharka (plum pox virus) in Serbia – previous research and future prospects for Its control. in Journal of Mountain Agriculture on the Balkans. 2019;22(2):162-176..

Jevremović, Darko, Paunović, Svetlana A., "Sharka (plum pox virus) in Serbia – previous research and future prospects for Its control" in Journal of Mountain Agriculture on the Balkans, 22, no. 2 (2019):162-176.

TY  - CONF
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/783
AB  - Plum pox virus (PPV, genus Potyvirus) is the causal agent of Sharka disease that is considered the most detrimental viral disease of stone fruits. Since its discovery in 1932 in Bulgaria, the disease has spread progressively to entire Europe and other continents, except Australia. PPV causes significant yield losses and reduces fruit quality in sensitive plum, peach and apricot cultivars. Sharka was reported in Serbia in 1935, and so far the presence of three major strains (PPV-M, -D and -Rec) was confirmed. Fifteen years ago, a largescale study on the presence, distribution, genetic diversity and epidemiology of Plum pox virus strains has begun at the Fruit Research Institute, Čačak. In this review, we summarized the results of our field and laboratory research and presented the future prospects for disease control in the country where Sharka is endemic to maintain feasible commercial fruit production.
AB  - Plum pox virus (PPV, род Potyvirus) е причинител на болестта шарка, която се счита за най-пагубното вирусно заболяване при костилковите видове. От своето откритие през 1932 г. в България болестта се разпространява прогресивно в цяла Европа и други континенти, с изключение на Австралия. PPV причинява значителни загуби в добива и понижава качеството на плодовете при чувствителни сортове сливи, праскови и кайсии. Шарката е установена в Сърбия през 1935 г. И досега е потвърдено наличието на три основни щама (PPV-M, -D и -Rec). Преди петнадесет години в Изследователски институт по овощарство, Чачак, започна широко мащабно проучване за наличието, разпространението, генетичното разнообразие и епидемиологията на щамовете на Plum pox virus. В настоящoто изследване са обобщени резултатите от наши полски и лабораторни изследвания, предста вени са бъдещи перспективи за контрол върху болестта в Сърбия, където шарка та е ендемична, за приемливо поддържане търговско производство на плодове.
PB  - Troyan : Research Institute of Mountain Stockbreeding and Agriculture
C3  - Book of Summaries : 22nd International Scientific Conference ‘EcoMountain - 2019’, May 16-17, 2019, Troyan, Bulgaria
T1  - Sharka (plum pox virus) in Serbia – previous research and future prospects for its control
T1  - Болестта шарка (plum pox virus) в Сърбия – предишни изследвания и бъдещи перспективи за контрол
EP  - 119
SP  - 119
ER  - 

@conference{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2019",
abstract = "Plum pox virus (PPV, genus Potyvirus) is the causal agent of Sharka disease that is considered the most detrimental viral disease of stone fruits. Since its discovery in 1932 in Bulgaria, the disease has spread progressively to entire Europe and other continents, except Australia. PPV causes significant yield losses and reduces fruit quality in sensitive plum, peach and apricot cultivars. Sharka was reported in Serbia in 1935, and so far the presence of three major strains (PPV-M, -D and -Rec) was confirmed. Fifteen years ago, a largescale study on the presence, distribution, genetic diversity and epidemiology of Plum pox virus strains has begun at the Fruit Research Institute, Čačak. In this review, we summarized the results of our field and laboratory research and presented the future prospects for disease control in the country where Sharka is endemic to maintain feasible commercial fruit production., Plum pox virus (PPV, род Potyvirus) е причинител на болестта шарка, която се счита за най-пагубното вирусно заболяване при костилковите видове. От своето откритие през 1932 г. в България болестта се разпространява прогресивно в цяла Европа и други континенти, с изключение на Австралия. PPV причинява значителни загуби в добива и понижава качеството на плодовете при чувствителни сортове сливи, праскови и кайсии. Шарката е установена в Сърбия през 1935 г. И досега е потвърдено наличието на три основни щама (PPV-M, -D и -Rec). Преди петнадесет години в Изследователски институт по овощарство, Чачак, започна широко мащабно проучване за наличието, разпространението, генетичното разнообразие и епидемиологията на щамовете на Plum pox virus. В настоящoто изследване са обобщени резултатите от наши полски и лабораторни изследвания, предста вени са бъдещи перспективи за контрол върху болестта в Сърбия, където шарка та е ендемична, за приемливо поддържане търговско производство на плодове.",
publisher = "Troyan : Research Institute of Mountain Stockbreeding and Agriculture",
journal = "Book of Summaries : 22nd International Scientific Conference ‘EcoMountain - 2019’, May 16-17, 2019, Troyan, Bulgaria",
title = "Sharka (plum pox virus) in Serbia – previous research and future prospects for its control, Болестта шарка (plum pox virus) в Сърбия – предишни изследвания и бъдещи перспективи за контрол",
pages = "119-119"
}

Jevremović, D.,& Paunović, S. A.. (2019). Sharka (plum pox virus) in Serbia – previous research and future prospects for its control. in Book of Summaries : 22nd International Scientific Conference ‘EcoMountain - 2019’, May 16-17, 2019, Troyan, Bulgaria
Troyan : Research Institute of Mountain Stockbreeding and Agriculture., 119-119.

Jevremović D, Paunović SA. Sharka (plum pox virus) in Serbia – previous research and future prospects for its control. in Book of Summaries : 22nd International Scientific Conference ‘EcoMountain - 2019’, May 16-17, 2019, Troyan, Bulgaria. 2019;:119-119..

Jevremović, Darko, Paunović, Svetlana A., "Sharka (plum pox virus) in Serbia – previous research and future prospects for its control" in Book of Summaries : 22nd International Scientific Conference ‘EcoMountain - 2019’, May 16-17, 2019, Troyan, Bulgaria (2019):119-119.

TY  - JOUR
AU  - Popović, Branko
AU  - Mitrović, Olga
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
AU  - Jevremović, Darko
AU  - Nikićević, Ninoslav
AU  - Tešević, Vele
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/410
AB  - During the two-year research, a comparative analysis of the contents of 24 major volatile compounds and sensory characteristics of the plum spirits produced by spontaneous alcoholic fermentation of the plum mashes with or without stones from cultivar Cacanska Rodna and its parent cultivars - Stanley and Pozegaca were carried out. The plum spirits obtained from Cacanska Rodna cultivar contain lower amounts of methanol, 1-hexanol, ethyl acetate and acetaldehyde and higher amounts of 3-methyl-1-butanol and 2-phenyl-ethanol than the spirits from its parent cultivars. Contents of 1-propanol, 1-butanol and hexanoic acid in the spirits obtained from Cacanska Rodna cultivar were lower than the contents in the spirits from Pozegaca and higher than those in the spirits obtained from Stanley. The sensory quality of plum spirits, in addition to the cultivar, has been significantly influenced by the presence of stones in the mash during processing of plums into spirits. In processing plums with stones, the best-graded plum spirit was from Pozegaca (17.88), whereas in processing without stones, the best was Cacanska Rodna spirit (17.78). The spirits obtained from Stanley cultivar had the lowest sensory grades regardless of the processing method.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Chemical and sensory characterization of plum spirits obtained from cultivar Cacanska Rodna and its parent cultivars
EP  - 1390
IS  - 12
SP  - 1381
VL  - 84
DO  - 10.2298/JSC190307061P
UR  - conv_1598
ER  - 

@article{
author = "Popović, Branko and Mitrović, Olga and Leposavić, Aleksandar and Paunović, Svetlana A. and Jevremović, Darko and Nikićević, Ninoslav and Tešević, Vele",
year = "2019",
abstract = "During the two-year research, a comparative analysis of the contents of 24 major volatile compounds and sensory characteristics of the plum spirits produced by spontaneous alcoholic fermentation of the plum mashes with or without stones from cultivar Cacanska Rodna and its parent cultivars - Stanley and Pozegaca were carried out. The plum spirits obtained from Cacanska Rodna cultivar contain lower amounts of methanol, 1-hexanol, ethyl acetate and acetaldehyde and higher amounts of 3-methyl-1-butanol and 2-phenyl-ethanol than the spirits from its parent cultivars. Contents of 1-propanol, 1-butanol and hexanoic acid in the spirits obtained from Cacanska Rodna cultivar were lower than the contents in the spirits from Pozegaca and higher than those in the spirits obtained from Stanley. The sensory quality of plum spirits, in addition to the cultivar, has been significantly influenced by the presence of stones in the mash during processing of plums into spirits. In processing plums with stones, the best-graded plum spirit was from Pozegaca (17.88), whereas in processing without stones, the best was Cacanska Rodna spirit (17.78). The spirits obtained from Stanley cultivar had the lowest sensory grades regardless of the processing method.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical and sensory characterization of plum spirits obtained from cultivar Cacanska Rodna and its parent cultivars",
pages = "1390-1381",
number = "12",
volume = "84",
doi = "10.2298/JSC190307061P",
url = "conv_1598"
}

Popović, B., Mitrović, O., Leposavić, A., Paunović, S. A., Jevremović, D., Nikićević, N.,& Tešević, V.. (2019). Chemical and sensory characterization of plum spirits obtained from cultivar Cacanska Rodna and its parent cultivars. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(12), 1381-1390.
https://doi.org/10.2298/JSC190307061P
conv_1598

Popović B, Mitrović O, Leposavić A, Paunović SA, Jevremović D, Nikićević N, Tešević V. Chemical and sensory characterization of plum spirits obtained from cultivar Cacanska Rodna and its parent cultivars. in Journal of the Serbian Chemical Society. 2019;84(12):1381-1390.
doi:10.2298/JSC190307061P
conv_1598 .

Popović, Branko, Mitrović, Olga, Leposavić, Aleksandar, Paunović, Svetlana A., Jevremović, Darko, Nikićević, Ninoslav, Tešević, Vele, "Chemical and sensory characterization of plum spirits obtained from cultivar Cacanska Rodna and its parent cultivars" in Journal of the Serbian Chemical Society, 84, no. 12 (2019):1381-1390,
https://doi.org/10.2298/JSC190307061P .,
conv_1598 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/408
AB  - Raspberry leaf blotch emaravirus (RLBV) is a recently characterised virus infecting raspberries reported in several European countries. RLBV causes yellow blotching, the distortion of leaf margins, and the twisting of raspberry leaves. For a long time, similar symptoms were attributed to the feeding damage caused by raspberry leaf and bud mite (Phyllocoptes gracilis). From 2014-2017, a large-scale survey was conducted in Serbia to investigate the degree of association of the observed symptoms with the RLBV infection. A total of 98 symptomatic and asymptomatic samples were collected from 30 locations. All collected samples were tested on the RLBV presence by reverse transcription and polymerase chain reaction (RT-PCR) using three sets of RNA-specific primers targeting RNA-1, RNA-3, and RNA-5 of the RLBV genome. RT-PCR analysis revealed high incidence of RLBV in tested samples (68.7%). RLBV was confirmed in raspberries 'Fertodi Zamatos', 'Glen Ample', 'Meeker', 'Polana', 'Tulameen' and 'Willamette'. Twenty-one isolates were selected for sequencing the portion of the nucleocapsid (NC) gene. The nucleotide sequences of the isolates showed 93.2-100% identity. Phylogenetic analysis confirmed significant genetic variability of the Serbian RLBV isolates based on the nucleocapsid-encoding sequences and revealed the existence of two main clusters. Phylogenetic analysis of the 45 RLBV sequences from Finland, Slovakia, Scotland, and this study also confirmed the existence of two main clusters of isolates.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia
IS  - 1
VL  - 17
DO  - 10.5424/sjar/2019171-13861
UR  - conv_1594
ER  - 

@article{
author = "Jevremović, Darko and Leposavić, Aleksandar and Paunović, Svetlana A.",
year = "2019",
abstract = "Raspberry leaf blotch emaravirus (RLBV) is a recently characterised virus infecting raspberries reported in several European countries. RLBV causes yellow blotching, the distortion of leaf margins, and the twisting of raspberry leaves. For a long time, similar symptoms were attributed to the feeding damage caused by raspberry leaf and bud mite (Phyllocoptes gracilis). From 2014-2017, a large-scale survey was conducted in Serbia to investigate the degree of association of the observed symptoms with the RLBV infection. A total of 98 symptomatic and asymptomatic samples were collected from 30 locations. All collected samples were tested on the RLBV presence by reverse transcription and polymerase chain reaction (RT-PCR) using three sets of RNA-specific primers targeting RNA-1, RNA-3, and RNA-5 of the RLBV genome. RT-PCR analysis revealed high incidence of RLBV in tested samples (68.7%). RLBV was confirmed in raspberries 'Fertodi Zamatos', 'Glen Ample', 'Meeker', 'Polana', 'Tulameen' and 'Willamette'. Twenty-one isolates were selected for sequencing the portion of the nucleocapsid (NC) gene. The nucleotide sequences of the isolates showed 93.2-100% identity. Phylogenetic analysis confirmed significant genetic variability of the Serbian RLBV isolates based on the nucleocapsid-encoding sequences and revealed the existence of two main clusters. Phylogenetic analysis of the 45 RLBV sequences from Finland, Slovakia, Scotland, and this study also confirmed the existence of two main clusters of isolates.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia",
number = "1",
volume = "17",
doi = "10.5424/sjar/2019171-13861",
url = "conv_1594"
}

Jevremović, D., Leposavić, A.,& Paunović, S. A.. (2019). Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia. in Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 17(1).
https://doi.org/10.5424/sjar/2019171-13861
conv_1594

Jevremović D, Leposavić A, Paunović SA. Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia. in Spanish Journal of Agricultural Research. 2019;17(1).
doi:10.5424/sjar/2019171-13861
conv_1594 .

Jevremović, Darko, Leposavić, Aleksandar, Paunović, Svetlana A., "Genetic diversity of Raspberry leaf blotch emaravirus in red raspberries from Serbia" in Spanish Journal of Agricultural Research, 17, no. 1 (2019),
https://doi.org/10.5424/sjar/2019171-13861 .,
conv_1594 .

TY  - JOUR
AU  - Jevremović, Darko
AU  - Paunović, Svetlana A.
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/396
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - First report of persimmon cryptic virus in persimmon in North Macedonia
EP  - 1285
IS  - 4
SP  - 1285
VL  - 101
DO  - 10.1007/s42161-019-00351-1
UR  - conv_1596
ER  - 

@article{
author = "Jevremović, Darko and Paunović, Svetlana A.",
year = "2019",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "First report of persimmon cryptic virus in persimmon in North Macedonia",
pages = "1285-1285",
number = "4",
volume = "101",
doi = "10.1007/s42161-019-00351-1",
url = "conv_1596"
}

Jevremović, D.,& Paunović, S. A.. (2019). First report of persimmon cryptic virus in persimmon in North Macedonia. in Journal of Plant Pathology
Springer, New York., 101(4), 1285-1285.
https://doi.org/10.1007/s42161-019-00351-1
conv_1596

Jevremović D, Paunović SA. First report of persimmon cryptic virus in persimmon in North Macedonia. in Journal of Plant Pathology. 2019;101(4):1285-1285.
doi:10.1007/s42161-019-00351-1
conv_1596 .

Jevremović, Darko, Paunović, Svetlana A., "First report of persimmon cryptic virus in persimmon in North Macedonia" in Journal of Plant Pathology, 101, no. 4 (2019):1285-1285,
https://doi.org/10.1007/s42161-019-00351-1 .,
conv_1596 .

TY  - JOUR
AU  - Popović, Branko
AU  - Mitrović, Olga
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
AU  - Jevremović, Darko
AU  - Nikićević, Ninoslav
AU  - Tešević, Vele
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/583
AB  - During the two-year research, a comparative analysis of the contents of 24 major volatile compounds and sensory characteristics of the plum spirits produced by spontaneous alcoholic fermentation of the plum mashes with or without stones from cultivar Čačanska Rodna and its parent cultivars – Stanley and Požegača were carried out. The plum spirits obtained from Čačanska Rodna cultivar contain lower amounts of methanol, 1-hexanol, ethyl acetate and acetaldehyde and higher amounts of 3-methyl-1-butanol and 2-phenylethanol than the spirits from its parent cultivars. Contents of 1-propanol, 1-butanol and hexanoic acid in the spirits obtained from Čačanska Rodna cultivar were lower than the contents in the spirits from Požegača and higher than those in the spirits obtained from Stanley. The sensory quality of plum spirits, in addition to the cultivar, has been significantly influenced by the presence of stones in the mash during processing of plums into spirits. In processing plums with stones, the best-graded plum spirit was from Požegača (17.88), whereas in processing without stones, the best was Čačanska Rodna spirit (17.78). The spirits obtained from Stanley cultivar had the lowest sensory grades regardless of the processing method.
AB  - Тоkоm dvоgоdišnjih ispitivаnjа, аnаlizirаni su sаdržајi 24 vаžniје ispаrlјivе kоmpоnеntе i sеnzоrnе kаrаktеristikе šlјivоvicа prоizvеdеnih оd spоntаnо fеrmеntisаnih klјukоvа šlјivе sа kоšticаmа ili bеz njih sоrtе čаčаnskа rоdnа i njеnih rоditеlјskih sоrаtа – stеnlеја i pоžеgаčе. Sоrtа šlјivе znаčајnо utičе nа hеmiјski sаstаv šlјivоvicа. Šlјivоvicе dоbiјеnе оd sоrtе čаčаnskа rоdnа sаdržаlе su mаnjе mеtаnоlа, 1-hеksаnоlа, еtilаcеtаtа i аcеtаldеhidа, а višе 3-mеtil-1-butаnоlа i 2-fеnilеtаnоlа nеgо šlјivоvicе prоizvеdеnе оd rоditеlјskih sоrаtа. Sаdržајi 1-prоpаnоlа, 1-butаnоlа i hеksаnskе kisеlinе u rаkiјаmа оd sоrtе čаčаnskа rоdnа bili su niži оd sаdržаја u šlјivоvicаmа оd sоrtе pоžеgаčа, а viši nеgо u šlјivоvicаmа dоbiјеnim оd sоrtе stеnlеј. Pоrеd sоrtе, nа sеnzоrni kvаlitеt šlјivоvicа је znаčајnо uticаlо i prisustvо kоšticа u klјuku tоkоm prеrаdе šlјivа u rаkiјu. Pri prеrаdi šlјivа sа kоšticаmа, nајbоlје је оcеnjеnа šlјivоvicа sоrtе pоžеgаčа (17,88), dоk је pri prеrаdi bеz kоšticа nајvišu оcеnu dоbilа šlјivоvicа sоrtе čаčаnskа rоdnа (17,78). Šlјivоvicе sоrtе stеnlеј dоbilе su nајnižе sеnzоrnе оcеnе, bеz оbzirа nа nаčin prеrаdе. Nа оsnоvu dоbiјеnih rеzultаtа mоžе sе zаklјučiti dа је sоrtа čačanska rodna pogodnа sirovinа za proizvodnju kvalitetne rakije šlјivovice.
PB  - Beograd : Srpsko hemijsko društvo
T2  - Journal of Serbian Chemical Society
T1  - Chemical and sensory characterization of plum spirits obtained from cultivar Čačanska Rodna and its parent cultivars
T1  - Hеmiјskа i sеnzоrnа kаrаktеrizаciја šlјivоvicа dоbiјеnih оd sоrtе Čаčаnskа rоdnа i njеnih rоditеlјskih sоrаtа
EP  - 1390
IS  - 12
SP  - 1381
VL  - 84
ER  - 

@article{
author = "Popović, Branko and Mitrović, Olga and Leposavić, Aleksandar and Paunović, Svetlana A. and Jevremović, Darko and Nikićević, Ninoslav and Tešević, Vele",
year = "2019",
abstract = "During the two-year research, a comparative analysis of the contents of 24 major volatile compounds and sensory characteristics of the plum spirits produced by spontaneous alcoholic fermentation of the plum mashes with or without stones from cultivar Čačanska Rodna and its parent cultivars – Stanley and Požegača were carried out. The plum spirits obtained from Čačanska Rodna cultivar contain lower amounts of methanol, 1-hexanol, ethyl acetate and acetaldehyde and higher amounts of 3-methyl-1-butanol and 2-phenylethanol than the spirits from its parent cultivars. Contents of 1-propanol, 1-butanol and hexanoic acid in the spirits obtained from Čačanska Rodna cultivar were lower than the contents in the spirits from Požegača and higher than those in the spirits obtained from Stanley. The sensory quality of plum spirits, in addition to the cultivar, has been significantly influenced by the presence of stones in the mash during processing of plums into spirits. In processing plums with stones, the best-graded plum spirit was from Požegača (17.88), whereas in processing without stones, the best was Čačanska Rodna spirit (17.78). The spirits obtained from Stanley cultivar had the lowest sensory grades regardless of the processing method., Тоkоm dvоgоdišnjih ispitivаnjа, аnаlizirаni su sаdržајi 24 vаžniје ispаrlјivе kоmpоnеntе i sеnzоrnе kаrаktеristikе šlјivоvicа prоizvеdеnih оd spоntаnо fеrmеntisаnih klјukоvа šlјivе sа kоšticаmа ili bеz njih sоrtе čаčаnskа rоdnа i njеnih rоditеlјskih sоrаtа – stеnlеја i pоžеgаčе. Sоrtа šlјivе znаčајnо utičе nа hеmiјski sаstаv šlјivоvicа. Šlјivоvicе dоbiјеnе оd sоrtе čаčаnskа rоdnа sаdržаlе su mаnjе mеtаnоlа, 1-hеksаnоlа, еtilаcеtаtа i аcеtаldеhidа, а višе 3-mеtil-1-butаnоlа i 2-fеnilеtаnоlа nеgо šlјivоvicе prоizvеdеnе оd rоditеlјskih sоrаtа. Sаdržајi 1-prоpаnоlа, 1-butаnоlа i hеksаnskе kisеlinе u rаkiјаmа оd sоrtе čаčаnskа rоdnа bili su niži оd sаdržаја u šlјivоvicаmа оd sоrtе pоžеgаčа, а viši nеgо u šlјivоvicаmа dоbiјеnim оd sоrtе stеnlеј. Pоrеd sоrtе, nа sеnzоrni kvаlitеt šlјivоvicа је znаčајnо uticаlо i prisustvо kоšticа u klјuku tоkоm prеrаdе šlјivа u rаkiјu. Pri prеrаdi šlјivа sа kоšticаmа, nајbоlје је оcеnjеnа šlјivоvicа sоrtе pоžеgаčа (17,88), dоk је pri prеrаdi bеz kоšticа nајvišu оcеnu dоbilа šlјivоvicа sоrtе čаčаnskа rоdnа (17,78). Šlјivоvicе sоrtе stеnlеј dоbilе su nајnižе sеnzоrnе оcеnе, bеz оbzirа nа nаčin prеrаdе. Nа оsnоvu dоbiјеnih rеzultаtа mоžе sе zаklјučiti dа је sоrtа čačanska rodna pogodnа sirovinа za proizvodnju kvalitetne rakije šlјivovice.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Journal of Serbian Chemical Society",
title = "Chemical and sensory characterization of plum spirits obtained from cultivar Čačanska Rodna and its parent cultivars, Hеmiјskа i sеnzоrnа kаrаktеrizаciја šlјivоvicа dоbiјеnih оd sоrtе Čаčаnskа rоdnа i njеnih rоditеlјskih sоrаtа",
pages = "1390-1381",
number = "12",
volume = "84"
}

Popović, B., Mitrović, O., Leposavić, A., Paunović, S. A., Jevremović, D., Nikićević, N.,& Tešević, V.. (2019). Chemical and sensory characterization of plum spirits obtained from cultivar Čačanska Rodna and its parent cultivars. in Journal of Serbian Chemical Society
Beograd : Srpsko hemijsko društvo., 84(12), 1381-1390.

Popović B, Mitrović O, Leposavić A, Paunović SA, Jevremović D, Nikićević N, Tešević V. Chemical and sensory characterization of plum spirits obtained from cultivar Čačanska Rodna and its parent cultivars. in Journal of Serbian Chemical Society. 2019;84(12):1381-1390..

Popović, Branko, Mitrović, Olga, Leposavić, Aleksandar, Paunović, Svetlana A., Jevremović, Darko, Nikićević, Ninoslav, Tešević, Vele, "Chemical and sensory characterization of plum spirits obtained from cultivar Čačanska Rodna and its parent cultivars" in Journal of Serbian Chemical Society, 84, no. 12 (2019):1381-1390.

TY  - CONF
AU  - Jevremović, Darko
AU  - Leposavić, Aleksandar
AU  - Paunović, Svetlana A.
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/570
AB  - Black raspberry necrosis virus (BRNV) is considered as economically important virus infecting raspberries. In black raspberry (Rubus occidentalis L.) BRNV is causing cane tip necrosis, but infected red raspberry (Rubus idaeus L.) cultivars in general do not express foliar symptoms.
From 2014−2018, a number of 105 samples from 9 red raspberry cultivars (‘Almira’, ’Fertödi Zamatos’, ’Glen Ample’, ’Meeker’, ‘San Rafael’, ’Polana’, ’Polka’, ’Tulameen’ and ‘Willamette’) were collected from different localities in Serbia. Samples were tested on the BRNV presence by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers targeting 417 bp nucleotide (nt) fragment of the RNA-dependent RNA polymerase (RdRp) region of RNA 1 of the BRNV genome. BNRV was detected in 19 samples in cultivars ’Fertödi Zamatos’, ’Polana’, ’Tulameen’ and ‘Willamette’.
A 417nt sequence of the RdRp was determined for 5 isolates. The nt sequences of these isolates were 94.2−99.7% identical. When compared to available sequences of BRNV isolates from North America and Europe, Serbian isolates showed significant divergence (80.0−82.2% of nt identity).
A portion of the BRNV infected plants of cultivar ’Fertödi Zamatos’ were further tested on indicator R. occidentalis by inarch bottle grafting. Two months after inoculation cane tip begun to curl down and became necrotic on all inoculated plants. Non-grafted control plants were symptomless.
PB  - Sarajevo : University of Sarajevo, Faculty of Agriculture and Food Sciences
C3  - Book of abstracts : 30th International Scientific-Expert Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, 25-27 September 2019
T1  - Molecular and biological characterization of Black raspberry necrosis virus on red raspberry in Serbia
EP  - 33
SP  - 33
ER  - 

@conference{
author = "Jevremović, Darko and Leposavić, Aleksandar and Paunović, Svetlana A.",
year = "2019",
abstract = "Black raspberry necrosis virus (BRNV) is considered as economically important virus infecting raspberries. In black raspberry (Rubus occidentalis L.) BRNV is causing cane tip necrosis, but infected red raspberry (Rubus idaeus L.) cultivars in general do not express foliar symptoms.From 2014−2018, a number of 105 samples from 9 red raspberry cultivars (‘Almira’, ’Fertödi Zamatos’, ’Glen Ample’, ’Meeker’, ‘San Rafael’, ’Polana’, ’Polka’, ’Tulameen’ and ‘Willamette’) were collected from different localities in Serbia. Samples were tested on the BRNV presence by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers targeting 417 bp nucleotide (nt) fragment of the RNA-dependent RNA polymerase (RdRp) region of RNA 1 of the BRNV genome. BNRV was detected in 19 samples in cultivars ’Fertödi Zamatos’, ’Polana’, ’Tulameen’ and ‘Willamette’.A 417nt sequence of the RdRp was determined for 5 isolates. The nt sequences of these isolates were 94.2−99.7% identical. When compared to available sequences of BRNV isolates from North America and Europe, Serbian isolates showed significant divergence (80.0−82.2% of nt identity).A portion of the BRNV infected plants of cultivar ’Fertödi Zamatos’ were further tested on indicator R. occidentalis by inarch bottle grafting. Two months after inoculation cane tip begun to curl down and became necrotic on all inoculated plants. Non-grafted control plants were symptomless.",
publisher = "Sarajevo : University of Sarajevo, Faculty of Agriculture and Food Sciences",
journal = "Book of abstracts : 30th International Scientific-Expert Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, 25-27 September 2019",
title = "Molecular and biological characterization of Black raspberry necrosis virus on red raspberry in Serbia",
pages = "33-33"
}

Jevremović, D., Leposavić, A.,& Paunović, S. A.. (2019). Molecular and biological characterization of Black raspberry necrosis virus on red raspberry in Serbia. in Book of abstracts : 30th International Scientific-Expert Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, 25-27 September 2019
Sarajevo : University of Sarajevo, Faculty of Agriculture and Food Sciences., 33-33.

Jevremović D, Leposavić A, Paunović SA. Molecular and biological characterization of Black raspberry necrosis virus on red raspberry in Serbia. in Book of abstracts : 30th International Scientific-Expert Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, 25-27 September 2019. 2019;:33-33..

Jevremović, Darko, Leposavić, Aleksandar, Paunović, Svetlana A., "Molecular and biological characterization of Black raspberry necrosis virus on red raspberry in Serbia" in Book of abstracts : 30th International Scientific-Expert Conference of Agriculture and Food Industry, Sarajevo, Bosnia and Herzegovina, 25-27 September 2019 (2019):33-33.

TY  - CONF
AU  - Paunović, Svetlana A
AU  - Jevremović, Darko
PY  - 2019
UR  - https://refri.institut-cacak.org/handle/123456789/540
AB  - Little cherry virus-1 (genus Velaravirus, family Closteroviridae; LChV-1) is considered an important
pathogen of cherries worldwide. Together with Little cherry virus-2 (LChV-2) it is associated with little
cherry disease. Infection of susceptible cherry cultivars with one or both viruses can lead to severe
reduction of fruit quality and loss in yields. It is graft transmissible virus with unknown vector. Over
the past twenty years, it has been found that other Prunus species are also hosts of the LChV-1, but
mainly without visible symptoms. Since there is no data on the presence of LChV-1 on P. armeniaca
and P. domestica in Serbia, except our preliminary results of its presence on P. avium and P. cerasus,
the main goal of this study was to analyze the presence of the LChV-1 on these hosts and to characterize
Serbian isolates by sequence and phylogenetic analyses.
Occurrence of LChV-1 in sweet and sour cherries, apricot and plum was examined over two
growing seasons (2017 and 2018). A total of 116 leaf samples were collected from orchards and nurseries
of CAC planting materials, out of which 62 were sweet cherry, 16 sour cherry, 21 apricot and 16
plum samples. Samples comprising of 35 varieties and 3 unknown cultivars were collected during
the summer season at 16 localities. Total RNAs extracted from the leaves were tested by RT-PCR using
LChV-1 specific primers (LCV1U16390/LCV1L16809) targeting an ORF8 fragment.
LChV-1 specific PCR product, DNA fragment of 419 bp, was detected in 11 out of 116 tested
Prunus samples (9.48%). Nine of the 62 P. avium (14.52%) and two of the 21 P. armeniaca (9.52%)
samples were infected with LChV-1. None of the tested P. cerasus and P. domestica was infected. PCR
amplicons generated from one selected apricot (LC1) and five sweet cherry isolates (LC2-6), were
bidirectionally sequenced (Macrogen, Amsterdam) and characterized. The nucleotide sequences of the 419 bp fragments confirmed the identity of the LChV-1 for all sequenced isolates LC1-6 (with
sequences identity between 76.3% and 100%) and revealed a high genetic variability among the
Serbian apricot and sweet cherry isolates (in the range of 22.1% to 23.9%). Serbian apricot LC1 isolate
grouped together with other LChV-1 apricot isolates from the GenBank and showed 98.1% nt identity
with two Czech isolates KX831095 and KX831097. Serbian cherry isolates shared sequence identities
in the range from 90.9% to 100%, grouped in two separate groups. Isolates LC2 and LC5 (100% identical)
and LC6 grouped together with cherry isolates from Bulgaria MG458879 (shearing 94.5% and
93.6% identity, respectively), sour cherry isolates from Poland HE580104 (98.9% and 97.8% identity)
and Japanese isolate originated from P. serrulata MG934545 (98.1% and 97.1% identity). The other
two cherry isolates LC3 i LC4 showing 99.2% nt identity were clustered in a separate subgroup within
the group with already mentioned cherry isolates.
Detection of LChV-1 in apricot and cherry imposes the need for phytosanitary control in the
production of planting material. To the best of our knowledge, this is the first report of confirmed
detection of LChV-1 on apricot and sweet cherry in Serbia.
PB  - Belgrade : Plant Protection Society of Serbia
C3  - Book of Аbstracts : VIII Congress on Pant Protection, Zlatibor, Serbia, November 25-29, 2019
T1  - Occurrence and characterization of Little cherry virus-1 in Serbian sweet cherry and apricot trees
EP  - 170
SP  - 169
ER  - 

@conference{
author = "Paunović, Svetlana A and Jevremović, Darko",
year = "2019",
abstract = "Little cherry virus-1 (genus Velaravirus, family Closteroviridae; LChV-1) is considered an importantpathogen of cherries worldwide. Together with Little cherry virus-2 (LChV-2) it is associated with littlecherry disease. Infection of susceptible cherry cultivars with one or both viruses can lead to severereduction of fruit quality and loss in yields. It is graft transmissible virus with unknown vector. Overthe past twenty years, it has been found that other Prunus species are also hosts of the LChV-1, butmainly without visible symptoms. Since there is no data on the presence of LChV-1 on P. armeniacaand P. domestica in Serbia, except our preliminary results of its presence on P. avium and P. cerasus,the main goal of this study was to analyze the presence of the LChV-1 on these hosts and to characterizeSerbian isolates by sequence and phylogenetic analyses.Occurrence of LChV-1 in sweet and sour cherries, apricot and plum was examined over twogrowing seasons (2017 and 2018). A total of 116 leaf samples were collected from orchards and nurseriesof CAC planting materials, out of which 62 were sweet cherry, 16 sour cherry, 21 apricot and 16plum samples. Samples comprising of 35 varieties and 3 unknown cultivars were collected duringthe summer season at 16 localities. Total RNAs extracted from the leaves were tested by RT-PCR usingLChV-1 specific primers (LCV1U16390/LCV1L16809) targeting an ORF8 fragment.LChV-1 specific PCR product, DNA fragment of 419 bp, was detected in 11 out of 116 testedPrunus samples (9.48%). Nine of the 62 P. avium (14.52%) and two of the 21 P. armeniaca (9.52%)samples were infected with LChV-1. None of the tested P. cerasus and P. domestica was infected. PCRamplicons generated from one selected apricot (LC1) and five sweet cherry isolates (LC2-6), werebidirectionally sequenced (Macrogen, Amsterdam) and characterized. The nucleotide sequences of the 419 bp fragments confirmed the identity of the LChV-1 for all sequenced isolates LC1-6 (withsequences identity between 76.3% and 100%) and revealed a high genetic variability among theSerbian apricot and sweet cherry isolates (in the range of 22.1% to 23.9%). Serbian apricot LC1 isolategrouped together with other LChV-1 apricot isolates from the GenBank and showed 98.1% nt identitywith two Czech isolates KX831095 and KX831097. Serbian cherry isolates shared sequence identitiesin the range from 90.9% to 100%, grouped in two separate groups. Isolates LC2 and LC5 (100% identical)and LC6 grouped together with cherry isolates from Bulgaria MG458879 (shearing 94.5% and93.6% identity, respectively), sour cherry isolates from Poland HE580104 (98.9% and 97.8% identity)and Japanese isolate originated from P. serrulata MG934545 (98.1% and 97.1% identity). The othertwo cherry isolates LC3 i LC4 showing 99.2% nt identity were clustered in a separate subgroup withinthe group with already mentioned cherry isolates.Detection of LChV-1 in apricot and cherry imposes the need for phytosanitary control in theproduction of planting material. To the best of our knowledge, this is the first report of confirmeddetection of LChV-1 on apricot and sweet cherry in Serbia.",
publisher = "Belgrade : Plant Protection Society of Serbia",
journal = "Book of Аbstracts : VIII Congress on Pant Protection, Zlatibor, Serbia, November 25-29, 2019",
title = "Occurrence and characterization of Little cherry virus-1 in Serbian sweet cherry and apricot trees",
pages = "170-169"
}

Paunović, S. A.,& Jevremović, D.. (2019). Occurrence and characterization of Little cherry virus-1 in Serbian sweet cherry and apricot trees. in Book of Аbstracts : VIII Congress on Pant Protection, Zlatibor, Serbia, November 25-29, 2019
Belgrade : Plant Protection Society of Serbia., 169-170.

Paunović SA, Jevremović D. Occurrence and characterization of Little cherry virus-1 in Serbian sweet cherry and apricot trees. in Book of Аbstracts : VIII Congress on Pant Protection, Zlatibor, Serbia, November 25-29, 2019. 2019;:169-170..

Paunović, Svetlana A, Jevremović, Darko, "Occurrence and characterization of Little cherry virus-1 in Serbian sweet cherry and apricot trees" in Book of Аbstracts : VIII Congress on Pant Protection, Zlatibor, Serbia, November 25-29, 2019 (2019):169-170.