TY  - JOUR
AU  - Vujović, Tatjana
AU  - Anđelić, Tatjana
AU  - Ružić, Đurđina
PY  - 2023
UR  - https://refri.institut-cacak.org/handle/123456789/518
AB  - We presented the results about preserving in vitro selected fruit genotypes by cold storage strategy and the capacity for following shoot multiplication. The investigation included five fruit genotypes: plum ʻPožegača’, cherry rootstocks (ʻGisela 5’ and ʻTabel Edabriz’), raspberry ʻMeeker’, and blackberry ʻČačanska Bestrna’. The best results were achieved with shoots of the plum cultivar ʿPožegačaʾ which can be successfully stored at 5°C up to 10 months, without subculturing producing viable shoots. Furthermore, a significant increase in the multiplication index (13.0) of this genotype was noticed especially in shoots cultivated for 21 days under standard growing conditions, followed by storage at 5°C in total darkness for six months. ʻGisela 5’ and ʻTabel Edabriz’ cherry rootstocks were successfully preserved for three months in the same manner, and the plants (100%) well recovered. After being stored in cold conditions, the multiplication index of shoots in both rootstocks exhibited an increase ranging from 1.7 to 4.8 times compared to those grown under standard conditions. The viability rate of in vitro shoots of the raspberry cultivar ʻMeeker’ in cold conditions was also high (>67%). In this genotype, as well as in both cherry rootstocks, slow growth storage was accompanied by slow nitrogen uptake from the medium. In addition, the multiplication potential of encapsulated raspberry ʻMeeker’ and blackberry ʻČačanska Bestrna’ and cold stored (5°C, darkness) shoot tips was evaluated. Encapsulated shoot tips of ʻČačanska Bestrna’ (18.8%) and ʻMeeker’ (6.3%) survived the monthly cold storage period and exhibited regrowth. Shoots of selected genotypes were capable of multiplication in successive subcultures after cold storage, and often the multiplication indexes were higher compared to the control shoots that were consistently grown under standard conditions in the growth chamber. These results represent a significant contribution to the revitalization and further development of the national fruit gene bank.
AB  - U ovom radu su prikazani rezultati in vitro čuvanja kultura voćaka korišćenjem strategije hladnog skladištenja i sposobnosti izdanaka za multiplikaciju nakon konzervacije. U istraživanja je bilo uključeno pet genotipova različitih vrsta voćaka: sorta šljive Požegača, vegetativne podloge za trešnju i višnju Gisela 5 i Tabel Edabriz, sorta maline Meeker i sorta kupine Čačanska bestrna. Sorta šljive Požegača je uspešno čuvana do 10 meseci, dajući izdanke sa visokim indeksom multiplikacije koji je bio posebno izražen kod izdanaka uzgajanih 21 dan u standardnim uslovima, a zatim skladištenih na 5°C u potpunom mraku tokom šest meseci. Podloge za kalemljenje trešnje i višnje Gisela 5 i Tabel Edabriz su takođe uspešno očuvane na isti način tokom tri meseca, a stopa preživljavanja in vitro izdanaka je iznosila 100%. Nakon skladištenja u hladnim uslovima, indeks multiplikacije izdanaka kod vegetativnih podloga Gisela 5 i Tabel Edabriz je bio povećan od 1,7 do 4,8 puta po redosledu u odnosu na izdanke uzgajane u standardnim uslovima. Stopa preživljavanja in vitro izdanaka maline sorte Meeker je takođe bila visoka (iznad 67%) u hladnim uslovima. Kod ovog genotipa, kao i kod obe vegetativne podloge, spor rast je pratio sporu apsorpciju azota iz podloge. Vrhovi izdanaka sorte maline Meeker i kupine Čačanska bestrna su takođe bili inkapsulirani u kalcijum-alginatne kuglice i čuvani u hladnim uslovima (na +5°C, u potpunom mraku). Samo 18,8% vrhova izdanaka sorte Čačanska bestrna i 6,3% vrhova izdanaka sorte Meeker, koji su bili inkapsulirani u kalcijum-alginatne kuglice, preživeli su jednomesečni period hladnog skladištenja i dali regeneraciju vijabilnih izdanaka u in vitro uslovima. Međutim, izdanci su bili sposobni za multiplikaciju u sukcesivnim subkulturama nakon regeneracije, a indeksi multiplikacije su bili veći u odnosu na kontrolne izdanke koji su konstantno gajeni u standardnim uslovima u komori za gajenje in vitro biljaka.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo
T1  - Evaluation of multiplication potential of cold stored shoot cultures of selected fruit genotypes
T1  - Praćenje kapaciteta za multiplikaciju izdanaka različitih voćaka nakon konzervacije primenom tehnike hladnog skladištenja in vitro
EP  - 46
IS  - 215-216
SP  - 37
VL  - 57
DO  - 10.18485/pomology.2023.57.215_216.4
ER  - 

@article{
author = "Vujović, Tatjana and Anđelić, Tatjana and Ružić, Đurđina",
year = "2023",
abstract = "We presented the results about preserving in vitro selected fruit genotypes by cold storage strategy and the capacity for following shoot multiplication. The investigation included five fruit genotypes: plum ʻPožegača’, cherry rootstocks (ʻGisela 5’ and ʻTabel Edabriz’), raspberry ʻMeeker’, and blackberry ʻČačanska Bestrna’. The best results were achieved with shoots of the plum cultivar ʿPožegačaʾ which can be successfully stored at 5°C up to 10 months, without subculturing producing viable shoots. Furthermore, a significant increase in the multiplication index (13.0) of this genotype was noticed especially in shoots cultivated for 21 days under standard growing conditions, followed by storage at 5°C in total darkness for six months. ʻGisela 5’ and ʻTabel Edabriz’ cherry rootstocks were successfully preserved for three months in the same manner, and the plants (100%) well recovered. After being stored in cold conditions, the multiplication index of shoots in both rootstocks exhibited an increase ranging from 1.7 to 4.8 times compared to those grown under standard conditions. The viability rate of in vitro shoots of the raspberry cultivar ʻMeeker’ in cold conditions was also high (>67%). In this genotype, as well as in both cherry rootstocks, slow growth storage was accompanied by slow nitrogen uptake from the medium. In addition, the multiplication potential of encapsulated raspberry ʻMeeker’ and blackberry ʻČačanska Bestrna’ and cold stored (5°C, darkness) shoot tips was evaluated. Encapsulated shoot tips of ʻČačanska Bestrna’ (18.8%) and ʻMeeker’ (6.3%) survived the monthly cold storage period and exhibited regrowth. Shoots of selected genotypes were capable of multiplication in successive subcultures after cold storage, and often the multiplication indexes were higher compared to the control shoots that were consistently grown under standard conditions in the growth chamber. These results represent a significant contribution to the revitalization and further development of the national fruit gene bank., U ovom radu su prikazani rezultati in vitro čuvanja kultura voćaka korišćenjem strategije hladnog skladištenja i sposobnosti izdanaka za multiplikaciju nakon konzervacije. U istraživanja je bilo uključeno pet genotipova različitih vrsta voćaka: sorta šljive Požegača, vegetativne podloge za trešnju i višnju Gisela 5 i Tabel Edabriz, sorta maline Meeker i sorta kupine Čačanska bestrna. Sorta šljive Požegača je uspešno čuvana do 10 meseci, dajući izdanke sa visokim indeksom multiplikacije koji je bio posebno izražen kod izdanaka uzgajanih 21 dan u standardnim uslovima, a zatim skladištenih na 5°C u potpunom mraku tokom šest meseci. Podloge za kalemljenje trešnje i višnje Gisela 5 i Tabel Edabriz su takođe uspešno očuvane na isti način tokom tri meseca, a stopa preživljavanja in vitro izdanaka je iznosila 100%. Nakon skladištenja u hladnim uslovima, indeks multiplikacije izdanaka kod vegetativnih podloga Gisela 5 i Tabel Edabriz je bio povećan od 1,7 do 4,8 puta po redosledu u odnosu na izdanke uzgajane u standardnim uslovima. Stopa preživljavanja in vitro izdanaka maline sorte Meeker je takođe bila visoka (iznad 67%) u hladnim uslovima. Kod ovog genotipa, kao i kod obe vegetativne podloge, spor rast je pratio sporu apsorpciju azota iz podloge. Vrhovi izdanaka sorte maline Meeker i kupine Čačanska bestrna su takođe bili inkapsulirani u kalcijum-alginatne kuglice i čuvani u hladnim uslovima (na +5°C, u potpunom mraku). Samo 18,8% vrhova izdanaka sorte Čačanska bestrna i 6,3% vrhova izdanaka sorte Meeker, koji su bili inkapsulirani u kalcijum-alginatne kuglice, preživeli su jednomesečni period hladnog skladištenja i dali regeneraciju vijabilnih izdanaka u in vitro uslovima. Međutim, izdanci su bili sposobni za multiplikaciju u sukcesivnim subkulturama nakon regeneracije, a indeksi multiplikacije su bili veći u odnosu na kontrolne izdanke koji su konstantno gajeni u standardnim uslovima u komori za gajenje in vitro biljaka.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo",
title = "Evaluation of multiplication potential of cold stored shoot cultures of selected fruit genotypes, Praćenje kapaciteta za multiplikaciju izdanaka različitih voćaka nakon konzervacije primenom tehnike hladnog skladištenja in vitro",
pages = "46-37",
number = "215-216",
volume = "57",
doi = "10.18485/pomology.2023.57.215_216.4"
}

Vujović, T., Anđelić, T.,& Ružić, Đ.. (2023). Evaluation of multiplication potential of cold stored shoot cultures of selected fruit genotypes. in Voćarstvo
Čačak : Naučno voćarsko društvo Srbije., 57(215-216), 37-46.
https://doi.org/10.18485/pomology.2023.57.215_216.4

Vujović T, Anđelić T, Ružić Đ. Evaluation of multiplication potential of cold stored shoot cultures of selected fruit genotypes. in Voćarstvo. 2023;57(215-216):37-46.
doi:10.18485/pomology.2023.57.215_216.4 .

Vujović, Tatjana, Anđelić, Tatjana, Ružić, Đurđina, "Evaluation of multiplication potential of cold stored shoot cultures of selected fruit genotypes" in Voćarstvo, 57, no. 215-216 (2023):37-46,
https://doi.org/10.18485/pomology.2023.57.215_216.4 . .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Anđelić, Tatjana
AU  - Jevremović, Darko
AU  - Vasilijević, Bojana
PY  - 2022
AB  - Krioprezervacija predstavlja savremenu metodu za dugotrajno čuvanje biljnog materijala na ultra niskim temperaturama (tečni azot, -196 °C). Ova metoda je komplementarna konvencionalnim metodama čuvanja jer značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima (ex situ). Program primene ove strategije za dugotrajno očuvanje biljnih genetičkih resursa iniciran je i u Institutu za voćarstvo, Čaćak u okviru Odeljenja za fiziologiju voćaka pre više od jedne decenije. Istraživanja su fokusirana na optimizaciju protokola za različite in vitro tehnike krioprezervacije koje se baziraju na direktnom potapanju biljnog materijala u tečni azot i njihovu primenu u čuvanju genetičkih resursa kontinentalnih voćaka. Primenjene tehnike podrazumavaju primenu hemijske (vitrifikacija, „droplet“ vitrifikacija, „V cryo-plate“) ili fizičke dehidratacije (inkapsulacija-dehidracija, „D cryo-plate“) biljnog materijala pre potapanja u tečni azot. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike i sorte kupine Čaćanska bestrna. Ispitivanje primene tehnike vitrifikacija kod vrsta roda Prunus i Malus pokazalo je da se značajno povećanje kapaciteta regeneracije vrhova izdanaka podloge za trešnju Gisela 5 i sorte jabuke Gala Must posle otapanja iz tečnog azota može postići variranjem dužine dehidratacije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije postignuta je ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidratacije i dužine osmotske rehidratacije kod predstavnika rodova Prunus (autohtone sorte šljive Crvena Ranka, Sitnica i Požegača, džanarika, podloga za tešnju Gisela 5), Malus (jabuka Gala Must) i Rubus (kupina Čačanska bestrna). Poslednjih godina, inicirana su takođe i istraživanja primene novorazvijenih „V cryo-plate“ i „D cryoplate“ tehnika krioprezervacije. Ove tehnike omogućavaju prevazila`enje izvesnih ograničenja vezanih za nedovoljnu dehidrataciju biljnog tkiva, oštećenje i gubitak biljnog materijala tokom pripreme za krioprezervacione protokole, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem navedenih tehnika uspešno su krioprezervirani vrhovi izdanaka različitih genotipova autohtonih šjiva (Crvena Ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge za trešnju Gisela 5, sorte jagode Clery, borovnice Toro i mongolske borovnice (Amelanchier alnifolia) koji su gajeni u uslovima kulture in vitro. Dosadašnji rezultati postignuti u ovim istraživanjima predstavljaju značajan doprinos uspostavljanju optimizirane tehnologije dugotrajnog čuvanja biljnog materijala primenom različitih tehnika krioprezervacije. Ovaj pregled istraživanja daje dobru osnovu za dalju optimizaciju protokola koje će biti moguće direktno primeniti u budući program uspostavljanja in vitro banke gena (germplazme) voćnih biljnih vrsta koje se gaje u Srbiji., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’, and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods. Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.
AB  - Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’,
and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods.
Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo
T1  - Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak
T1  - Long-term conservation of fruit tree germplasm using different in vitro cryopreservation techniques at Fruit Research Institute, Čačak
EP  - 92
IS  - 213-214
SP  - 79
VL  - 56
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Anđelić, Tatjana and Jevremović, Darko and Vasilijević, Bojana",
year = "2022",
abstract = "Krioprezervacija predstavlja savremenu metodu za dugotrajno čuvanje biljnog materijala na ultra niskim temperaturama (tečni azot, -196 °C). Ova metoda je komplementarna konvencionalnim metodama čuvanja jer značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima (ex situ). Program primene ove strategije za dugotrajno očuvanje biljnih genetičkih resursa iniciran je i u Institutu za voćarstvo, Čaćak u okviru Odeljenja za fiziologiju voćaka pre više od jedne decenije. Istraživanja su fokusirana na optimizaciju protokola za različite in vitro tehnike krioprezervacije koje se baziraju na direktnom potapanju biljnog materijala u tečni azot i njihovu primenu u čuvanju genetičkih resursa kontinentalnih voćaka. Primenjene tehnike podrazumavaju primenu hemijske (vitrifikacija, „droplet“ vitrifikacija, „V cryo-plate“) ili fizičke dehidratacije (inkapsulacija-dehidracija, „D cryo-plate“) biljnog materijala pre potapanja u tečni azot. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike i sorte kupine Čaćanska bestrna. Ispitivanje primene tehnike vitrifikacija kod vrsta roda Prunus i Malus pokazalo je da se značajno povećanje kapaciteta regeneracije vrhova izdanaka podloge za trešnju Gisela 5 i sorte jabuke Gala Must posle otapanja iz tečnog azota može postići variranjem dužine dehidratacije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije postignuta je ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidratacije i dužine osmotske rehidratacije kod predstavnika rodova Prunus (autohtone sorte šljive Crvena Ranka, Sitnica i Požegača, džanarika, podloga za tešnju Gisela 5), Malus (jabuka Gala Must) i Rubus (kupina Čačanska bestrna). Poslednjih godina, inicirana su takođe i istraživanja primene novorazvijenih „V cryo-plate“ i „D cryoplate“ tehnika krioprezervacije. Ove tehnike omogućavaju prevazila`enje izvesnih ograničenja vezanih za nedovoljnu dehidrataciju biljnog tkiva, oštećenje i gubitak biljnog materijala tokom pripreme za krioprezervacione protokole, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem navedenih tehnika uspešno su krioprezervirani vrhovi izdanaka različitih genotipova autohtonih šjiva (Crvena Ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge za trešnju Gisela 5, sorte jagode Clery, borovnice Toro i mongolske borovnice (Amelanchier alnifolia) koji su gajeni u uslovima kulture in vitro. Dosadašnji rezultati postignuti u ovim istraživanjima predstavljaju značajan doprinos uspostavljanju optimizirane tehnologije dugotrajnog čuvanja biljnog materijala primenom različitih tehnika krioprezervacije. Ovaj pregled istraživanja daje dobru osnovu za dalju optimizaciju protokola koje će biti moguće direktno primeniti u budući program uspostavljanja in vitro banke gena (germplazme) voćnih biljnih vrsta koje se gaje u Srbiji., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’, and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods. Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country., Cryopreservation represents contemporary method for long-term conservation of plant germplasm at ultralow temperature (liquid nitrogen, -196oC). Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become a complementary approach to conventional conservation methods. Program for application of this strategy was also initiated within Department of Fruit Physiology of FRI, Čačak more than a decade ago. Most of the research has been focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving temperate fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of cherry plum and blackberry ‘Čačanska Bestrna’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’,and ‘Krina’, cherry plum, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’, ‘Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), cherry plum, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon (Amelanchier alnifolia) were successfully cryopreserved using these methods.Results obtained in this research represent a substantial contribution to establishing optimized conservation technology by application of different vitrification based cryopreservation techniques, as well as a good basis for further optimization of the protocols that will be possible to directly implement in the future program of establishing an in vitro germplasm bank of fruit tree species in our country.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo",
title = "Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak, Long-term conservation of fruit tree germplasm using different in vitro cryopreservation techniques at Fruit Research Institute, Čačak",
pages = "92-79",
number = "213-214",
volume = "56"
}

Vujović, T., Ružić, Đ., Anđelić, T., Jevremović, D.,& Vasilijević, B.. (2022). Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak. in Voćarstvo
Čačak : Naučno voćarsko društvo Srbije., 56(213-214), 79-92.

Vujović T, Ružić Đ, Anđelić T, Jevremović D, Vasilijević B. Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak. in Voćarstvo. 2022;56(213-214):79-92..

Vujović, Tatjana, Ružić, Đurđina, Anđelić, Tatjana, Jevremović, Darko, Vasilijević, Bojana, "Dugotrajno čuvanje germplazme voćaka primenom in vitro tehnika za krioprezervaciju vrhova izdanaka u Institutu za voćarstvo, Čačak" in Voćarstvo, 56, no. 213-214 (2022):79-92.

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Anđelić, Tatjana
AU  - Jevremović, Darko
AU  - Radičević, Sanja
AU  - Đorđević, Milena
PY  - 2022
UR  - https://refri.institut-cacak.org/handle/123456789/512
AB  - Čuvanje biljnog materijala na izuzetno niskim temperaturama (tečni azot, -196°C) je veoma značajana metoda za dugotrajnu konzervaciju biljne germplazme. Pošto krioprezervacija značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima, ova metoda je prepoznata kao komplementarana konvencionalnim metodama čuvanja. Program primene ovakve strategije u očuvanju biljnih genetičkih resursa je iniciran i u Institutu za voćarstvo, Čačak. Istraživanja su fokusirana na optimizaciju protokola za različite tehnike krioprezervacije koje se baziraju na fenomenu vitrifikacije i na njihovu primenu u konzervaciji germpazme kontinentalnih voćaka. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike, kupine Čačanska bestrna i maline ‘Latham’. Ispitivanje primene tehnike vitrifikacija u konzervaciji germplazme Prunus-a i Malus-a je pokazalo da se značajno povećanje kapaciteta regeneracije krioprezerviranih vrhova izdanaka podloge za trešnju ‘Gisela 5’ i jabuke ‘Gala Must’ može postići variranjem dužine dehidracije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije kod predstavnika rodova Prunus (šljive Crvena ranka, Sitnica, Požegača, Krina, Džanarika i podloga ‘Gisela 5’), Malus (jabuka ‘Gala Must’) i Rubus (kupina Čačanska bestrna) je postignuta ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidracije i dužine rehidracije na ponovno rastenje krioprezerviranih eksplantata. Poslednjih godina, inicirana su takođe i istraživanja primene novo razvijenih „V cryo-plate“ i „D cryo-plate“ metoda krioprezervacije. Ove metode omogućavaju prevazilaženje izvesnih ograničenja vezanih za nedovoljnu dehidraciju, oštećenje i gubitak biljnog materijala, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem ovih metoda uspešno su krioprezervirani vrhovi in vitro izdanaka različitih autohtonih šljiva (Crvena ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge ‘Gisela 5’, jagode ‘Clery’, borovnice ‘Toro’ i mongolske borovnice. Pored krioprezervacije in vitro eksplantata, u Institutu se vrše i ispitivanja uticaja kriotretmana na reproduktivnu sposobnost polena in vitro i in vivo kod različitih sorti šljive (Valjevka, Valerija, Čačanska lepotica) i trešnje (‘Kordia’, ‘Summit’).
AB  - Storage of plant material at ultra-low temperature (liquid nitrogen, -196°C), has become important tool for long-term conservation of plant germplasm. Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become а complementary approach to conventional conservation methods. Program for application of this strategy has also been initiated within Department of Fruit Physiology of FRI, Čačak. Most of the research is focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of myrobalan, blackberry ‘Čačanska Bestrna’ and raspberry ‘Latham’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’ and ‘Krina’, myrobalan, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’,‘ Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), myrobalan, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon were successfully cryopreserved using these methods. Besides cryopreservation of in vitro explants, the effect of cryo-treatment on pollen reproductive ability in vitro and in vivo has also been examined in different plum (‘Valjevka’, ‘Valerija’, ‘Čačanska Lepotica’) and sweet cherry (‘Kordia’, ‘Summit’) cultivars.
PB  - Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet
C3  - Zbornik apstrakata : 16. kongres voćara i vinogradara srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbija
T1  - Dugotrajna konzervacija germplazme voćaka primenom različitih tehnika krioprezervacije u Institutu za voćarstvo, Čačak
T1  - Long-term conservation of fruit tree germplasm using different cryopreservation techniques at Fruit Research Institute, Čačak
EP  - 121
SP  - 118
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Anđelić, Tatjana and Jevremović, Darko and Radičević, Sanja and Đorđević, Milena",
year = "2022",
abstract = "Čuvanje biljnog materijala na izuzetno niskim temperaturama (tečni azot, -196°C) je veoma značajana metoda za dugotrajnu konzervaciju biljne germplazme. Pošto krioprezervacija značajno snižava troškove i rizike vezane za održavanje biljnih kolekcija u poljskim uslovima, ova metoda je prepoznata kao komplementarana konvencionalnim metodama čuvanja. Program primene ovakve strategije u očuvanju biljnih genetičkih resursa je iniciran i u Institutu za voćarstvo, Čačak. Istraživanja su fokusirana na optimizaciju protokola za različite tehnike krioprezervacije koje se baziraju na fenomenu vitrifikacije i na njihovu primenu u konzervaciji germpazme kontinentalnih voćaka. Tehnika inkapsulacije-dehidracije je uspešno primenjena u krioprezervaciji džanarike, kupine Čačanska bestrna i maline ‘Latham’. Ispitivanje primene tehnike vitrifikacija u konzervaciji germplazme Prunus-a i Malus-a je pokazalo da se značajno povećanje kapaciteta regeneracije krioprezerviranih vrhova izdanaka podloge za trešnju ‘Gisela 5’ i jabuke ‘Gala Must’ može postići variranjem dužine dehidracije i/ili vrste korišćenih vitrifikacionih rastvora. Optimizacija protokola za tehniku „droplet“ vitrifikacije kod predstavnika rodova Prunus (šljive Crvena ranka, Sitnica, Požegača, Krina, Džanarika i podloga ‘Gisela 5’), Malus (jabuka ‘Gala Must’) i Rubus (kupina Čačanska bestrna) je postignuta ispitivanjem uticaja različitih vitrifikacionih rastvora, dužine tretmana, temperature dehidracije i dužine rehidracije na ponovno rastenje krioprezerviranih eksplantata. Poslednjih godina, inicirana su takođe i istraživanja primene novo razvijenih „V cryo-plate“ i „D cryo-plate“ metoda krioprezervacije. Ove metode omogućavaju prevazilaženje izvesnih ograničenja vezanih za nedovoljnu dehidraciju, oštećenje i gubitak biljnog materijala, kao i manipulativnih problema u protokolima drugih tehnika vitrifikacije. Korišćenjem ovih metoda uspešno su krioprezervirani vrhovi in vitro izdanaka različitih autohtonih šljiva (Crvena ranka, Požegača, Sitnica, Moravka, Belošljiva), džanarike, podloge ‘Gisela 5’, jagode ‘Clery’, borovnice ‘Toro’ i mongolske borovnice. Pored krioprezervacije in vitro eksplantata, u Institutu se vrše i ispitivanja uticaja kriotretmana na reproduktivnu sposobnost polena in vitro i in vivo kod različitih sorti šljive (Valjevka, Valerija, Čačanska lepotica) i trešnje (‘Kordia’, ‘Summit’)., Storage of plant material at ultra-low temperature (liquid nitrogen, -196°C), has become important tool for long-term conservation of plant germplasm. Since cryopreservation reduces cost and risks linked to management of field germplasm collections, it has become а complementary approach to conventional conservation methods. Program for application of this strategy has also been initiated within Department of Fruit Physiology of FRI, Čačak. Most of the research is focused on optimization of the protocols for different vitrification-based cryopreservation techniques and their application for conserving fruit tree genotypes. Encapsulation-dehydration technique has been successfully applied for cryopreservation of myrobalan, blackberry ‘Čačanska Bestrna’ and raspberry ‘Latham’. Research on application of vitrification technique for conservation of Prunus and Malus germplasm has shown that significant increase in regrowth of cryopreserved shoot tips of cherry rootstock ‘Gisela 5’ and apple ‘Gala Must’ can be achieved by varying duration of dehydration and/or type of vitrification solution (VS) employed. Optimization of the droplet-vitrification protocol in representatives of Prunus (plums ‘Crvena Ranka’, ‘Sitnica’, ‘Požegača’ and ‘Krina’, myrobalan, rootstock ‘Gisela 5’), Malus (apple ‘Gala Must’) and Rubus (blackberry ‘Čačanska Bestrna’) genera was performed by evaluating the effect of different VSs, treatment durations, temperature of dehydration and duration of unloading on recovery of cryopreserved explants. Recently, the research on application of newly developed V and D cryo-plate techniques has been initiated, too. These methods allow overcoming disadvantages of other vitrification procedures such as mistiming dehydration, damage and loss of material and manipulative problems. Shoot tips of number of autochthonous plums (‘Crvena Ranka’,‘ Požegača’, ‘Sitnica’, ‘Moravka’, ‘Belošljiva’), myrobalan, rootstock ‘Gisela 5’, strawberry ‘Clery’, blueberry ‘Toro’ and saskatoon were successfully cryopreserved using these methods. Besides cryopreservation of in vitro explants, the effect of cryo-treatment on pollen reproductive ability in vitro and in vivo has also been examined in different plum (‘Valjevka’, ‘Valerija’, ‘Čačanska Lepotica’) and sweet cherry (‘Kordia’, ‘Summit’) cultivars.",
publisher = "Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet",
journal = "Zbornik apstrakata : 16. kongres voćara i vinogradara srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbija",
title = "Dugotrajna konzervacija germplazme voćaka primenom različitih tehnika krioprezervacije u Institutu za voćarstvo, Čačak, Long-term conservation of fruit tree germplasm using different cryopreservation techniques at Fruit Research Institute, Čačak",
pages = "121-118"
}

Vujović, T., Ružić, Đ., Anđelić, T., Jevremović, D., Radičević, S.,& Đorđević, M.. (2022). Dugotrajna konzervacija germplazme voćaka primenom različitih tehnika krioprezervacije u Institutu za voćarstvo, Čačak. in Zbornik apstrakata : 16. kongres voćara i vinogradara srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbija
Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet., 118-121.

Vujović T, Ružić Đ, Anđelić T, Jevremović D, Radičević S, Đorđević M. Dugotrajna konzervacija germplazme voćaka primenom različitih tehnika krioprezervacije u Institutu za voćarstvo, Čačak. in Zbornik apstrakata : 16. kongres voćara i vinogradara srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbija. 2022;:118-121..

Vujović, Tatjana, Ružić, Đurđina, Anđelić, Tatjana, Jevremović, Darko, Radičević, Sanja, Đorđević, Milena, "Dugotrajna konzervacija germplazme voćaka primenom različitih tehnika krioprezervacije u Institutu za voćarstvo, Čačak" in Zbornik apstrakata : 16. kongres voćara i vinogradara srbije sa međunarodnim učešćem, 28. februar – 03. mart 2022. godine, Vrdnik, Republika Srbija (2022):118-121.

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Jevremović, Darko
AU  - Marjanović, Tatjana
AU  - Ružić, Đurđina
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/463
AB  - Vujovic T., D. Jevremovic, T. Marjanovic, D. Ruzic (2021). Cryopreservation of Serbian autochthonous plum 'Crvena Ranka' using aluminium cryo-plates. - Genetika, Vol 53, No.1, 283 -294. 'Crvena Ranka' is one of the oldest autochthonous plum cultivars in Serbia. However, it is endangered due to the long-term pathogen pressure and continuous suppression by commercially important cultivars and therefore faced with genetic erosion. This study was carried out to investigate the suitability of two efficient and simple cryopreservation methods using aluminium cryo-plates for its conservation. Precultured shoot tips of this genotype were embedded in alginate gel into cryo-plates wells. Osmoprotection was performed using loading solution with 1.9 M glycerol and 0.5 M sucrose (30 min at room temperature). In the V cryo-plate protocol, explants were dehydrated at room temperature for 20 or 40 min with PVS A3 (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or with PVS3 (50% glycerol and 50% sucrose) for 60 min. In the D cryo-plate protocol, desiccation for 2, 2.5 or 3 h was performed over silica gel. Then, the cryo-plates were directly immersed into liquid nitrogen. Unloading was done in MS medium containing 0.8 M sucrose (30 min at room temperature). In the V cryo-plate procedure regrowth of cryopreserved explants dehydrated with PVS A3 was between 50% and 51.9%, while in those dehydrated with PVS3 it was 66.7%. As for the D cryo-plate method, regrowth of cryopreserved explants ranged between 30-40%. After regrowth, shoots were successfully multiplied and rooted. Results prove the feasibility of these new cryogenic methods for a long-term storage of this valuable Prunus genotype.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Cryopreservation of Serbian autochthonous plum 'Crvena ranka' using aluminium cryo-plates
EP  - 294
IS  - 1
SP  - 283
VL  - 53
DO  - 10.2298/GENSR2101283V
UR  - conv_1615
ER  - 

@article{
author = "Vujović, Tatjana and Jevremović, Darko and Marjanović, Tatjana and Ružić, Đurđina",
year = "2021",
abstract = "Vujovic T., D. Jevremovic, T. Marjanovic, D. Ruzic (2021). Cryopreservation of Serbian autochthonous plum 'Crvena Ranka' using aluminium cryo-plates. - Genetika, Vol 53, No.1, 283 -294. 'Crvena Ranka' is one of the oldest autochthonous plum cultivars in Serbia. However, it is endangered due to the long-term pathogen pressure and continuous suppression by commercially important cultivars and therefore faced with genetic erosion. This study was carried out to investigate the suitability of two efficient and simple cryopreservation methods using aluminium cryo-plates for its conservation. Precultured shoot tips of this genotype were embedded in alginate gel into cryo-plates wells. Osmoprotection was performed using loading solution with 1.9 M glycerol and 0.5 M sucrose (30 min at room temperature). In the V cryo-plate protocol, explants were dehydrated at room temperature for 20 or 40 min with PVS A3 (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or with PVS3 (50% glycerol and 50% sucrose) for 60 min. In the D cryo-plate protocol, desiccation for 2, 2.5 or 3 h was performed over silica gel. Then, the cryo-plates were directly immersed into liquid nitrogen. Unloading was done in MS medium containing 0.8 M sucrose (30 min at room temperature). In the V cryo-plate procedure regrowth of cryopreserved explants dehydrated with PVS A3 was between 50% and 51.9%, while in those dehydrated with PVS3 it was 66.7%. As for the D cryo-plate method, regrowth of cryopreserved explants ranged between 30-40%. After regrowth, shoots were successfully multiplied and rooted. Results prove the feasibility of these new cryogenic methods for a long-term storage of this valuable Prunus genotype.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Cryopreservation of Serbian autochthonous plum 'Crvena ranka' using aluminium cryo-plates",
pages = "294-283",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101283V",
url = "conv_1615"
}

Vujović, T., Jevremović, D., Marjanović, T.,& Ružić, Đ.. (2021). Cryopreservation of Serbian autochthonous plum 'Crvena ranka' using aluminium cryo-plates. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 283-294.
https://doi.org/10.2298/GENSR2101283V
conv_1615

Vujović T, Jevremović D, Marjanović T, Ružić Đ. Cryopreservation of Serbian autochthonous plum 'Crvena ranka' using aluminium cryo-plates. in Genetika-Belgrade. 2021;53(1):283-294.
doi:10.2298/GENSR2101283V
conv_1615 .

Vujović, Tatjana, Jevremović, Darko, Marjanović, Tatjana, Ružić, Đurđina, "Cryopreservation of Serbian autochthonous plum 'Crvena ranka' using aluminium cryo-plates" in Genetika-Belgrade, 53, no. 1 (2021):283-294,
https://doi.org/10.2298/GENSR2101283V .,
conv_1615 .

TY  - CONF
AU  - Mitrović, Olga
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Popović, Branko
AU  - Leposavić, Aleksandar
AU  - Karaklajić-Stajić, Žaklina
AU  - Korićanac, Aleksandra
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/506
AB  - Berry fruits are a valuable source of phytochemicals, primarily sugars, organic acids and phenolic compounds. Since sugars and organic acids are the most abundant soluble solids, they greatly determine fruit’s taste, which is one of the most important parameters for consumers’ acceptance. Due to the high content of naturally occurring antioxidants, particularly flavonoids, phenolic acids and anthocyanins, berries are considered as an important source of health-promoting compounds. The Republic of Serbia is one of the leading producers of raspberry and blackberry in Europe. Although high yield and satisfactory fruit quality largely depend on agroecological conditions, they cannot be reached without high-quality planting material. Raspberry and blackberry are mostly propagated vegetatively by conventional methods. However, micropropagation enables a year-round supply of physiologically uniform, true-to-type and disease-free planting material. Thus, we established the orchard using planting material propagated by standard technique (ST) and by in vitro tissue culture (TC). Three-year study evaluated the fruit quality of raspberry ‘Meeker’ and blackberry ‘Čačanska Bestrna’, considering the different origins of the planting material. Several chemical parameters of fruit quality (dry matter, content of sugars, total acids content, pH, total pectins) were analysed. High-performance liquid chromatography was used for the determination of phenolic acids (protocatechuic, 4-hydroxybenzoic, ellagic, gallic, p-coumaric, caffeic and ferulic acid), flavonols (quercetin) and anthocyanins (cyanidin and pelargonidin). No significant differences were observed between berries from the ST and TC plants in both fruit species regarding any analysed chemical parameter. As expected, the most abundant phenolic acid in all berry samples was ellagic acid. With exception of 4-hydroxybenzoic acid in blackberry, the type of planting material did not significantly affect the content of bioactive compounds in both blackberry and raspberry. On the other side, experimental year significantly influenced the abovementioned compounds, excluding the content of ellagic acid, 4-hydroxybenzoic acid and gallic acid in raspberry.
PB  - Belgrade : University of Belgrade
C3  - Book of Abstracts : Unifood conference, Belgrade, September 24-25, 2021
T1  - Chemical and phenolic composition of fruits of raspberry and blackberry propagated by standard and in vitro techniques
EP  - 117
SP  - 117
ER  - 

@conference{
author = "Mitrović, Olga and Ružić, Đurđina and Vujović, Tatjana and Popović, Branko and Leposavić, Aleksandar and Karaklajić-Stajić, Žaklina and Korićanac, Aleksandra",
year = "2021",
abstract = "Berry fruits are a valuable source of phytochemicals, primarily sugars, organic acids and phenolic compounds. Since sugars and organic acids are the most abundant soluble solids, they greatly determine fruit’s taste, which is one of the most important parameters for consumers’ acceptance. Due to the high content of naturally occurring antioxidants, particularly flavonoids, phenolic acids and anthocyanins, berries are considered as an important source of health-promoting compounds. The Republic of Serbia is one of the leading producers of raspberry and blackberry in Europe. Although high yield and satisfactory fruit quality largely depend on agroecological conditions, they cannot be reached without high-quality planting material. Raspberry and blackberry are mostly propagated vegetatively by conventional methods. However, micropropagation enables a year-round supply of physiologically uniform, true-to-type and disease-free planting material. Thus, we established the orchard using planting material propagated by standard technique (ST) and by in vitro tissue culture (TC). Three-year study evaluated the fruit quality of raspberry ‘Meeker’ and blackberry ‘Čačanska Bestrna’, considering the different origins of the planting material. Several chemical parameters of fruit quality (dry matter, content of sugars, total acids content, pH, total pectins) were analysed. High-performance liquid chromatography was used for the determination of phenolic acids (protocatechuic, 4-hydroxybenzoic, ellagic, gallic, p-coumaric, caffeic and ferulic acid), flavonols (quercetin) and anthocyanins (cyanidin and pelargonidin). No significant differences were observed between berries from the ST and TC plants in both fruit species regarding any analysed chemical parameter. As expected, the most abundant phenolic acid in all berry samples was ellagic acid. With exception of 4-hydroxybenzoic acid in blackberry, the type of planting material did not significantly affect the content of bioactive compounds in both blackberry and raspberry. On the other side, experimental year significantly influenced the abovementioned compounds, excluding the content of ellagic acid, 4-hydroxybenzoic acid and gallic acid in raspberry.",
publisher = "Belgrade : University of Belgrade",
journal = "Book of Abstracts : Unifood conference, Belgrade, September 24-25, 2021",
title = "Chemical and phenolic composition of fruits of raspberry and blackberry propagated by standard and in vitro techniques",
pages = "117-117"
}

Mitrović, O., Ružić, Đ., Vujović, T., Popović, B., Leposavić, A., Karaklajić-Stajić, Ž.,& Korićanac, A.. (2021). Chemical and phenolic composition of fruits of raspberry and blackberry propagated by standard and in vitro techniques. in Book of Abstracts : Unifood conference, Belgrade, September 24-25, 2021
Belgrade : University of Belgrade., 117-117.

Mitrović O, Ružić Đ, Vujović T, Popović B, Leposavić A, Karaklajić-Stajić Ž, Korićanac A. Chemical and phenolic composition of fruits of raspberry and blackberry propagated by standard and in vitro techniques. in Book of Abstracts : Unifood conference, Belgrade, September 24-25, 2021. 2021;:117-117..

Mitrović, Olga, Ružić, Đurđina, Vujović, Tatjana, Popović, Branko, Leposavić, Aleksandar, Karaklajić-Stajić, Žaklina, Korićanac, Aleksandra, "Chemical and phenolic composition of fruits of raspberry and blackberry propagated by standard and in vitro techniques" in Book of Abstracts : Unifood conference, Belgrade, September 24-25, 2021 (2021):117-117.

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
PY  - 2021
UR  - https://refri.institut-cacak.org/handle/123456789/459
AB  - This paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype.
PB  - International Society for Horticultural Science, Leuven 1
C3  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future
T1  - Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration
EP  - 40
SP  - 33
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.6
UR  - conv_1623
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav",
year = "2021",
abstract = "This paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype.",
publisher = "International Society for Horticultural Science, Leuven 1",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future",
title = "Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration",
pages = "40-33",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.6",
url = "conv_1623"
}

Vujović, T., Ružić, Đ.,& Cerović, R.. (2021). Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future
International Society for Horticultural Science, Leuven 1., 1308, 33-40.
https://doi.org/10.17660/ActaHortic.2021.1308.6
conv_1623

Vujović T, Ružić Đ, Cerović R. Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future. 2021;1308:33-40.
doi:10.17660/ActaHortic.2021.1308.6
conv_1623 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits For the Future, 1308 (2021):33-40,
https://doi.org/10.17660/ActaHortic.2021.1308.6 .,
conv_1623 .

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Vranić, Dragana
AU  - Marjanović, Tatjana
PY  - 2020
UR  - https://refri.institut-cacak.org/handle/123456789/419
AB  - The aim of this study was to develop an efficient and rapid droplet-vitrification protocol to cryopreserve in vitro shoot tips of Malus x domestica Borkh. 'Gala Must'. Following excision, shoot tips were pre-cultured in liquid Murashige and Skoog (MS) medium enriched with sucrose at two concentrations (0.3 M for 15 h, then 0.7 M for 5 h). Loading took place at room temperature in a solution containing 1.9 M glycerol and 0.5 M sucrose for 30 min. Explants were dehydrated using either modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO, w/v) or PVS3 solution (50% sucrose and 50% glycerol, w/v). PVS A3-based dehydration was done for 30, 40 and 50 min either at room temperature or at 0 degrees C. As regards PVS3, explants were dehydrated at room temperature for 40, 50, 60, 90 and 120 min. Regrowth of the cryopreserved shoot tips dehydrated at room temperature with PVS A3 solution ranged between 0 and 14.5%, the highest rate being achieved with the shortest treatment duration. Dehydration with the same VS at 0 degrees C resulted in a considerably higher regrowth capacity of cryopreserved shoots (45-70%), the best results were achieved with 40-min treatment. Regrowth of PVS3-treated shoot tips (40-60 min) did not markedly vary (45-50%), while prolonged dehydration (90-120 min) resulted in a significant decrease of regeneration capacity of cryopreserved shoot tips (12.1-5%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3-based dehydration at 0 degrees C for a long-term storage of this genotype using droplet-vitrification technique, although good results were also achieved with shorter PVS3 treatments at room temperature.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Cryopreservation in vitro of apple shoot tips following droplet-vitrification
EP  - 8
SP  - 1
VL  - 1289
DO  - 10.17660/ActaHortic.2020.1289.1
UR  - conv_1668
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Vranić, Dragana and Marjanović, Tatjana",
year = "2020",
abstract = "The aim of this study was to develop an efficient and rapid droplet-vitrification protocol to cryopreserve in vitro shoot tips of Malus x domestica Borkh. 'Gala Must'. Following excision, shoot tips were pre-cultured in liquid Murashige and Skoog (MS) medium enriched with sucrose at two concentrations (0.3 M for 15 h, then 0.7 M for 5 h). Loading took place at room temperature in a solution containing 1.9 M glycerol and 0.5 M sucrose for 30 min. Explants were dehydrated using either modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO, w/v) or PVS3 solution (50% sucrose and 50% glycerol, w/v). PVS A3-based dehydration was done for 30, 40 and 50 min either at room temperature or at 0 degrees C. As regards PVS3, explants were dehydrated at room temperature for 40, 50, 60, 90 and 120 min. Regrowth of the cryopreserved shoot tips dehydrated at room temperature with PVS A3 solution ranged between 0 and 14.5%, the highest rate being achieved with the shortest treatment duration. Dehydration with the same VS at 0 degrees C resulted in a considerably higher regrowth capacity of cryopreserved shoots (45-70%), the best results were achieved with 40-min treatment. Regrowth of PVS3-treated shoot tips (40-60 min) did not markedly vary (45-50%), while prolonged dehydration (90-120 min) resulted in a significant decrease of regeneration capacity of cryopreserved shoot tips (12.1-5%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3-based dehydration at 0 degrees C for a long-term storage of this genotype using droplet-vitrification technique, although good results were also achieved with shorter PVS3 treatments at room temperature.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Cryopreservation in vitro of apple shoot tips following droplet-vitrification",
pages = "8-1",
volume = "1289",
doi = "10.17660/ActaHortic.2020.1289.1",
url = "conv_1668"
}

Vujović, T., Ružić, Đ., Vranić, D.,& Marjanović, T.. (2020). Cryopreservation in vitro of apple shoot tips following droplet-vitrification. in Acta Horticulturae
International Society for Horticultural Science., 1289, 1-8.
https://doi.org/10.17660/ActaHortic.2020.1289.1
conv_1668

Vujović T, Ružić Đ, Vranić D, Marjanović T. Cryopreservation in vitro of apple shoot tips following droplet-vitrification. in Acta Horticulturae. 2020;1289:1-8.
doi:10.17660/ActaHortic.2020.1289.1
conv_1668 .

Vujović, Tatjana, Ružić, Đurđina, Vranić, Dragana, Marjanović, Tatjana, "Cryopreservation in vitro of apple shoot tips following droplet-vitrification" in Acta Horticulturae, 1289 (2020):1-8,
https://doi.org/10.17660/ActaHortic.2020.1289.1 .,
conv_1668 .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Marjanović, Tatjana
AU  - Ružić, Đurđina
AU  - Glišić, Ivana
PY  - 2018
UR  - https://refri.institut-cacak.org/handle/123456789/507
AB  - To determine the effect of successive subculturing on multiplication capacity of shoots, three rootstocks for plum, i.e. Cherry plum (Prunus cerasifera Ehrh.), ‘Docera 6’ (P. domestica × P. cerasifera) and ‘Dospina 235’ (P. domestica × P. spinosa) were repeatedly subcultured for five subcultures on Murashige & Skoog (MS) medium of unchanged plant growth regulator composition. Monitoring of the shoot formation capacity revealed constant increase in multiplication index and length of axial and lateral shoots in Cherry plum during each consecutive multiplication cycle. As for ‘Docera’ and ‘Dospina’, significan increase in multiplication index and length of axial shoots was observed in the third subculture. After that their values gradually decreased to the fifth subculture, but remained considerabely higher in comparison with the values in the first two subcultures. This study has confirmed that response of in vitro cultures to constant subculturing is genotype dependant. Considering that the decline in multiplication index was already observed after third subculture, it is neccesary to determine when cytokinin type and concentration should be reduced, or the hormon-free medium should be deployed to delay the decrease. MS medium with mineral salts reduced to ½-strength and organic complex unchanged was used in rooting stage. The highest capacity for in vitro rooting was observed in Cherry plum (100%), followed by ‘Docera’ (91.7%), while the lowest was in ‘Dospina’ (51.9%). Therefore, an additional optimization of this stage by testing other types and concentration of auxin should be done in order to achieve higher rooting percentage in ‘Dospina’.
AB  - Tri podloge za šljivu, džanarika (Prunus cerasifera Ehrh.), Docera 6 (P. domestica × P. cerasifera) i Dospina 235 (P. domestica × P. spinosa) su umnožavane in vitro tokom pet uzastopnih supkultura na Murashige & Skoog (MS) hranljivoj podlozi konstantnog sadržaja biljnih regulatora rastenja u cilju ispitivanja uticaja sukcesivnog supkultivisanja na parametre multiplikacije. Praćenjem regenerativnog kapaciteta utvrđeno je konstantno povećanje indeksa multiplikacije, dužine osovinskih i bo~nih izdanaka kod podloge džanarika. Kod podloga Docera i Dospina uočeno je značajno povećanje indeksa multiplikacije i dužine osovinskih izdanaka u trećoj supkulturi, posle čega njihova vrednost postepeno opada do pete supkulture, ali i dalje ostaje značajno veća u odnosu na prve dve supkulture. Ovi rezultati potvrđuju činjenicu da uticaj supkultivisanja na kapacitet za multiplikaciju in vitro značajno varira u zavisnosti od genotipa. Takođe, uzimajući u obzir da je kod podloga Docera i Dospina pad regenerativnog kapaciteta izražen, kroz indeks multiplikacije, uočen već posle treće supkulture, neophodno je precizno definisati da li se i kada vrsta i koncentracija primenjenih citokinina mora promeniti ili pak upotrebiti hranljiva podloga bez hormona u cilju odlaganja ili prevazilaženja ovog smanjenja. U fazi ožiljavanja je korišćena MS hranljiva podloga sa mineralnim solima smanjenim na ½ i organskim kompleksom nepromenjenim prema MS. Najveći kapacitet ožiljavanja in vitro utvrđen je kod podloge džanarika (100%), pa zatim kod podloge Docera (91,7%), a najniži kod podloge Dospina (51.9%). Stoga je kod podloge Dospina potrebno izvršiti dodatnu optimizaciju ove faze testiranjem drugih vrsta auksina u cilju postizanja većih procenata ožiljavanja.
PB  - Čačak : Naučno voćarsko društvo Srbije
T2  - Voćarstvo : Journal of Pomology
T1  - In vitro propagation of plum rootstocks
T1  - In vitro razmnožavanje podloga za šljivu
EP  - 97
IS  - 203-204
SP  - 91
VL  - 52
ER  - 

@article{
author = "Vujović, Tatjana and Marjanović, Tatjana and Ružić, Đurđina and Glišić, Ivana",
year = "2018",
abstract = "To determine the effect of successive subculturing on multiplication capacity of shoots, three rootstocks for plum, i.e. Cherry plum (Prunus cerasifera Ehrh.), ‘Docera 6’ (P. domestica × P. cerasifera) and ‘Dospina 235’ (P. domestica × P. spinosa) were repeatedly subcultured for five subcultures on Murashige & Skoog (MS) medium of unchanged plant growth regulator composition. Monitoring of the shoot formation capacity revealed constant increase in multiplication index and length of axial and lateral shoots in Cherry plum during each consecutive multiplication cycle. As for ‘Docera’ and ‘Dospina’, significan increase in multiplication index and length of axial shoots was observed in the third subculture. After that their values gradually decreased to the fifth subculture, but remained considerabely higher in comparison with the values in the first two subcultures. This study has confirmed that response of in vitro cultures to constant subculturing is genotype dependant. Considering that the decline in multiplication index was already observed after third subculture, it is neccesary to determine when cytokinin type and concentration should be reduced, or the hormon-free medium should be deployed to delay the decrease. MS medium with mineral salts reduced to ½-strength and organic complex unchanged was used in rooting stage. The highest capacity for in vitro rooting was observed in Cherry plum (100%), followed by ‘Docera’ (91.7%), while the lowest was in ‘Dospina’ (51.9%). Therefore, an additional optimization of this stage by testing other types and concentration of auxin should be done in order to achieve higher rooting percentage in ‘Dospina’., Tri podloge za šljivu, džanarika (Prunus cerasifera Ehrh.), Docera 6 (P. domestica × P. cerasifera) i Dospina 235 (P. domestica × P. spinosa) su umnožavane in vitro tokom pet uzastopnih supkultura na Murashige & Skoog (MS) hranljivoj podlozi konstantnog sadržaja biljnih regulatora rastenja u cilju ispitivanja uticaja sukcesivnog supkultivisanja na parametre multiplikacije. Praćenjem regenerativnog kapaciteta utvrđeno je konstantno povećanje indeksa multiplikacije, dužine osovinskih i bo~nih izdanaka kod podloge džanarika. Kod podloga Docera i Dospina uočeno je značajno povećanje indeksa multiplikacije i dužine osovinskih izdanaka u trećoj supkulturi, posle čega njihova vrednost postepeno opada do pete supkulture, ali i dalje ostaje značajno veća u odnosu na prve dve supkulture. Ovi rezultati potvrđuju činjenicu da uticaj supkultivisanja na kapacitet za multiplikaciju in vitro značajno varira u zavisnosti od genotipa. Takođe, uzimajući u obzir da je kod podloga Docera i Dospina pad regenerativnog kapaciteta izražen, kroz indeks multiplikacije, uočen već posle treće supkulture, neophodno je precizno definisati da li se i kada vrsta i koncentracija primenjenih citokinina mora promeniti ili pak upotrebiti hranljiva podloga bez hormona u cilju odlaganja ili prevazilaženja ovog smanjenja. U fazi ožiljavanja je korišćena MS hranljiva podloga sa mineralnim solima smanjenim na ½ i organskim kompleksom nepromenjenim prema MS. Najveći kapacitet ožiljavanja in vitro utvrđen je kod podloge džanarika (100%), pa zatim kod podloge Docera (91,7%), a najniži kod podloge Dospina (51.9%). Stoga je kod podloge Dospina potrebno izvršiti dodatnu optimizaciju ove faze testiranjem drugih vrsta auksina u cilju postizanja većih procenata ožiljavanja.",
publisher = "Čačak : Naučno voćarsko društvo Srbije",
journal = "Voćarstvo : Journal of Pomology",
title = "In vitro propagation of plum rootstocks, In vitro razmnožavanje podloga za šljivu",
pages = "97-91",
number = "203-204",
volume = "52"
}

Vujović, T., Marjanović, T., Ružić, Đ.,& Glišić, I.. (2018). In vitro propagation of plum rootstocks. in Voćarstvo : Journal of Pomology
Čačak : Naučno voćarsko društvo Srbije., 52(203-204), 91-97.

Vujović T, Marjanović T, Ružić Đ, Glišić I. In vitro propagation of plum rootstocks. in Voćarstvo : Journal of Pomology. 2018;52(203-204):91-97..

Vujović, Tatjana, Marjanović, Tatjana, Ružić, Đurđina, Glišić, Ivana, "In vitro propagation of plum rootstocks" in Voćarstvo : Journal of Pomology, 52, no. 203-204 (2018):91-97.

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Marjanović, Tatjana
PY  - 2018
UR  - https://refri.institut-cacak.org/handle/123456789/508
AB  - Vegetativne podloge za šljivu Docera, Dospina i St. Julien A su umnožavane in vitro pet uzastopnih supkultura na medijumu konstantnog sastava u cilju ispitivanja uticaja sukcesivnog supkultivisanja na parametre multiplikacije. Kod podloga Docera i Dospina uočeno je značajno povećanje indeksa multiplikacije i dužine osovinskih izdanaka u 3. supkulturi, posle čega njihova vrednost postepeno opada do 5. supkulture, ali i dalje ostaje značajno veća u odnosu na prve dve supkulture. Suprotno, kod podloge St. Julien A uočen je trend pada indeksa multiplikacije od 1. ka 3. supkulturi, posle čega njegova vrednost ponovo raste i ostaje na nivou 1. supkulture. Dužina osovinskih izdanaka se tokom supkultivisanjamenjala na isti način kao kod podloga Docera i Dospina. Najveći kapacitet ožiljavanja in vitro utvrđen je kod podloge Docera (91,7%), a najniži kod St. Julien A (44,4%).
AB  - To determine the effect of successive subculturing on multiplication capacity of shoots, vegetative rootstocks for plum Docera, Dospina and St. Julien A were repeatedly subcultured for 5 subcultures on medium of unchanged composition. As for Docera and Dospina, significan increase in multiplication index and length of axial shoots was observed in the 3. subculture, whereupon their values gradually decreased to the 5. subculture, but remained considerabely higher in comparison with the values in the first two subcultures. Contrary, in St. Julien A shoot multiplication declined over the first three subcultures, then increased and remained at the level of the 1. subculture. Length of axial shoots was changed in the same way as for Docera and Dospina. The highest cacpacity for in vitro rooting was observed in Docera (91.7%), while the lowest was in St. Julien A (44.4%).
PB  - Čačak : Univerzitet u Kragujevcu, Agronomski fakultet
C3  - Zbornik radova : XXIII savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 9-10. mart 2018. godine
T1  - In vitro razmnožavanje novih vegetativnih podloga za šlјivu
T1  - In vitro propagation of contemporary vegetative rootstocks for plum
EP  - 202
SP  - 197
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Marjanović, Tatjana",
year = "2018",
abstract = "Vegetativne podloge za šljivu Docera, Dospina i St. Julien A su umnožavane in vitro pet uzastopnih supkultura na medijumu konstantnog sastava u cilju ispitivanja uticaja sukcesivnog supkultivisanja na parametre multiplikacije. Kod podloga Docera i Dospina uočeno je značajno povećanje indeksa multiplikacije i dužine osovinskih izdanaka u 3. supkulturi, posle čega njihova vrednost postepeno opada do 5. supkulture, ali i dalje ostaje značajno veća u odnosu na prve dve supkulture. Suprotno, kod podloge St. Julien A uočen je trend pada indeksa multiplikacije od 1. ka 3. supkulturi, posle čega njegova vrednost ponovo raste i ostaje na nivou 1. supkulture. Dužina osovinskih izdanaka se tokom supkultivisanjamenjala na isti način kao kod podloga Docera i Dospina. Najveći kapacitet ožiljavanja in vitro utvrđen je kod podloge Docera (91,7%), a najniži kod St. Julien A (44,4%)., To determine the effect of successive subculturing on multiplication capacity of shoots, vegetative rootstocks for plum Docera, Dospina and St. Julien A were repeatedly subcultured for 5 subcultures on medium of unchanged composition. As for Docera and Dospina, significan increase in multiplication index and length of axial shoots was observed in the 3. subculture, whereupon their values gradually decreased to the 5. subculture, but remained considerabely higher in comparison with the values in the first two subcultures. Contrary, in St. Julien A shoot multiplication declined over the first three subcultures, then increased and remained at the level of the 1. subculture. Length of axial shoots was changed in the same way as for Docera and Dospina. The highest cacpacity for in vitro rooting was observed in Docera (91.7%), while the lowest was in St. Julien A (44.4%).",
publisher = "Čačak : Univerzitet u Kragujevcu, Agronomski fakultet",
journal = "Zbornik radova : XXIII savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 9-10. mart 2018. godine",
title = "In vitro razmnožavanje novih vegetativnih podloga za šlјivu, In vitro propagation of contemporary vegetative rootstocks for plum",
pages = "202-197"
}

Vujović, T., Ružić, Đ.,& Marjanović, T.. (2018). In vitro razmnožavanje novih vegetativnih podloga za šlјivu. in Zbornik radova : XXIII savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 9-10. mart 2018. godine
Čačak : Univerzitet u Kragujevcu, Agronomski fakultet., 197-202.

Vujović T, Ružić Đ, Marjanović T. In vitro razmnožavanje novih vegetativnih podloga za šlјivu. in Zbornik radova : XXIII savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 9-10. mart 2018. godine. 2018;:197-202..

Vujović, Tatjana, Ružić, Đurđina, Marjanović, Tatjana, "In vitro razmnožavanje novih vegetativnih podloga za šlјivu" in Zbornik radova : XXIII savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 9-10. mart 2018. godine (2018):197-202.

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
AU  - Ružić, Đurđina
AU  - Marjanović, Tatjana
PY  - 2018
UR  - https://refri.institut-cacak.org/handle/123456789/503
AB  - The most important aspect of in vitro propagation technology is to verify genetic fidelity of tissue culture-raised plants. In the present study, three techniques were employed to assess genetic integrity of micropropagated shoots of Pyrodwarf (Pyrus communis L.) pear rootstock. In vitro shoots derived either from organized meristems (axillary origin) or from leaf explants (adventitious origin). Flow cytometry analysis deployed to estimate the DNA ploidy level revealed no significant differences in nuclear DNA content among adventitious regeneration derived shoots, in vitro shoots of axillary origin and in vivo control plants from open field. Chromosome counting in root tip meristems also showed a normal diploid chromosome number (2n = 2x = 34) in both type of in vitro shoots. However, polyacrylamide gel electrophoresis of peroxidases (POX) extracted from the leaf tissue revealed the same POX banding patterns in open field mother plants and in vitro shoots of axillary origin, while adventitious-derived in vitro shoots exhibited polymorphism in POX profiles depending on the specific cytokinin/auxin combination used for adventitious regeneration. The obtained results indicate that although tissue culture plants maintained gross genetic stability at cytogenetic level, further investigation using powerful DNA markers is required, especially in adventitious regeneration derived shoots.
AB  - Най-важният аспект при in vitro размножаването е да се провери генетичната сигурност на тъканните култури. В настоящото изследване са използвани три техники за оценка на генетичната еднаквост на растения получени чрез микроразмножение от подложка за круша Pyrodwarf (Pyrus communis L.). In vitro леторастите са получени от диференцирани меристеми (с аксиларен произход) и листни експланти (адвентивна издънкова регенерация). Направеният анализ, за оценка нивото на плоидност на ДНК, чрез поточна цитометрия, не показва значими разлики в съдържанието на ядрената ДНК, между леторасти от адвентивна издънкова регенерация, in vitro леторасти от аксиларен произход и in vivo контролни растения, отглеждани на открито. Преброяването на хромозоми във връхна коренова меристема също показва нормален брой диплоидни хромозоми (2n = 2x = 34) и при двата вида in vitro леторасти. Въпреки това, електрофорезата с полиакриламиден гел на пероксидаза (POX), от листната тъкан, разкрива същите POX модели, като при майчини растения отглеждани на открито и in vitro леторастите от аксиларен произход, докато леторастите с адвентивна издънкова регенерация показват аксиларен полиморфизъм в POX профилите, в зависимост от специфичната цитокинин/ауксинова комбинация,използвана за адвентивна издънкова регенерация. Получените резултати показват, че въпреки че тъканните култури поддържат обща генетична стабилност на цитогенетично ниво, е необходимо допълнително изследване с мощни ДНК маркери, особено при леторасти с адвентивна издънкова регенерация.
PB  - Troyan, Bulgaria : Research Institute of Mountain Stockbreeding and Agriculture (RIMSA)
T2  - Journal of Mountain Agriculture on the Balkans
T1  - An assessment of genetic integrity of in vitro shoots of Pyrodwarf pear rootstock
T1  - Оценка на генетичната идентичност на in vitro подложки за круша Pyrodwarf
EP  - 182
IS  - 4
SP  - 166
VL  - 21
ER  - 

@article{
author = "Vujović, Tatjana and Cerović, Radosav and Ružić, Đurđina and Marjanović, Tatjana",
year = "2018",
abstract = "The most important aspect of in vitro propagation technology is to verify genetic fidelity of tissue culture-raised plants. In the present study, three techniques were employed to assess genetic integrity of micropropagated shoots of Pyrodwarf (Pyrus communis L.) pear rootstock. In vitro shoots derived either from organized meristems (axillary origin) or from leaf explants (adventitious origin). Flow cytometry analysis deployed to estimate the DNA ploidy level revealed no significant differences in nuclear DNA content among adventitious regeneration derived shoots, in vitro shoots of axillary origin and in vivo control plants from open field. Chromosome counting in root tip meristems also showed a normal diploid chromosome number (2n = 2x = 34) in both type of in vitro shoots. However, polyacrylamide gel electrophoresis of peroxidases (POX) extracted from the leaf tissue revealed the same POX banding patterns in open field mother plants and in vitro shoots of axillary origin, while adventitious-derived in vitro shoots exhibited polymorphism in POX profiles depending on the specific cytokinin/auxin combination used for adventitious regeneration. The obtained results indicate that although tissue culture plants maintained gross genetic stability at cytogenetic level, further investigation using powerful DNA markers is required, especially in adventitious regeneration derived shoots., Най-важният аспект при in vitro размножаването е да се провери генетичната сигурност на тъканните култури. В настоящото изследване са използвани три техники за оценка на генетичната еднаквост на растения получени чрез микроразмножение от подложка за круша Pyrodwarf (Pyrus communis L.). In vitro леторастите са получени от диференцирани меристеми (с аксиларен произход) и листни експланти (адвентивна издънкова регенерация). Направеният анализ, за оценка нивото на плоидност на ДНК, чрез поточна цитометрия, не показва значими разлики в съдържанието на ядрената ДНК, между леторасти от адвентивна издънкова регенерация, in vitro леторасти от аксиларен произход и in vivo контролни растения, отглеждани на открито. Преброяването на хромозоми във връхна коренова меристема също показва нормален брой диплоидни хромозоми (2n = 2x = 34) и при двата вида in vitro леторасти. Въпреки това, електрофорезата с полиакриламиден гел на пероксидаза (POX), от листната тъкан, разкрива същите POX модели, като при майчини растения отглеждани на открито и in vitro леторастите от аксиларен произход, докато леторастите с адвентивна издънкова регенерация показват аксиларен полиморфизъм в POX профилите, в зависимост от специфичната цитокинин/ауксинова комбинация,използвана за адвентивна издънкова регенерация. Получените резултати показват, че въпреки че тъканните култури поддържат обща генетична стабилност на цитогенетично ниво, е необходимо допълнително изследване с мощни ДНК маркери, особено при леторасти с адвентивна издънкова регенерация.",
publisher = "Troyan, Bulgaria : Research Institute of Mountain Stockbreeding and Agriculture (RIMSA)",
journal = "Journal of Mountain Agriculture on the Balkans",
title = "An assessment of genetic integrity of in vitro shoots of Pyrodwarf pear rootstock, Оценка на генетичната идентичност на in vitro подложки за круша Pyrodwarf",
pages = "182-166",
number = "4",
volume = "21"
}

Vujović, T., Cerović, R., Ružić, Đ.,& Marjanović, T.. (2018). An assessment of genetic integrity of in vitro shoots of Pyrodwarf pear rootstock. in Journal of Mountain Agriculture on the Balkans
Troyan, Bulgaria : Research Institute of Mountain Stockbreeding and Agriculture (RIMSA)., 21(4), 166-182.

Vujović T, Cerović R, Ružić Đ, Marjanović T. An assessment of genetic integrity of in vitro shoots of Pyrodwarf pear rootstock. in Journal of Mountain Agriculture on the Balkans. 2018;21(4):166-182..

Vujović, Tatjana, Cerović, Radosav, Ružić, Đurđina, Marjanović, Tatjana, "An assessment of genetic integrity of in vitro shoots of Pyrodwarf pear rootstock" in Journal of Mountain Agriculture on the Balkans, 21, no. 4 (2018):166-182.

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Marjanović, Tatjana
AU  - Cerović, Radosav
PY  - 2018
UR  - https://refri.institut-cacak.org/handle/123456789/502
AB  - In this work we employed two vitrification-based techniques (vitrification and droplet vitrification) to cryopreserve in vitro grown shoot tips of apple ‘Gala Must’ (Malus × domestica Borkh.). After preculture, shoot tips were osmoprotected at room temperature in a solution containing 2 M glycerol and 0.4 M sucrose for 20 min, and then dehydrated in the following plant vitrification solutions: PVS2 (13.7% sucrose, 30% glycerol, 15% ethylene glycol, 15% DMSO), PVS A3 (22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30, 40 and 50 min at 0 °C and PVS3 (50% glycerol and 50% sucrose) for 40, 50 and 60 min at room temperature. Explants dehydrated with PVS2 and PVS A3 were cryopreserved by vitrification while those dehydrated with PVS A3 and PVS3 were cryopreserved using droplet vitrification. In vitrification protocol, regrowth of the cryopreserved shoot tips dehydrated with PVS2 ranged between 20–40%. Dehydration with PVS A3 resulted in considerably higher regrowth rates (15–75%) using the same protocol. The highest values of regrowth were achieved with the longest treatment duration (50 min) for both vitrification solutions. As for droplet vitrification, regrowth of cryopreserved explants dehydrated with PVS A3 varied between 45–70%, and between 45–50% for those dehydrated with PVS3. The highest regrowth values, 70% and 50%, were achieved after 40-min PVS A3 treatment and 50-min PVS3 treatment, respectively. These results prove the feasibility of the PVS A3-based vitrification for the long-term storage of this genotype.
PB  - Belgrade : Serbian Plant Physiology Society
PB  - Belgrade : University of Belgrade, Institute for Biological Research “Siniša Stanković”
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - Book of Abstracts : 3rd International Conference on Plant Biology and 22nd SPPS Meeting, 9-12 June 2018, Belgrade
T1  - Cryopreservation of apple
EP  - 97
SP  - 97
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Marjanović, Tatjana and Cerović, Radosav",
year = "2018",
abstract = "In this work we employed two vitrification-based techniques (vitrification and droplet vitrification) to cryopreserve in vitro grown shoot tips of apple ‘Gala Must’ (Malus × domestica Borkh.). After preculture, shoot tips were osmoprotected at room temperature in a solution containing 2 M glycerol and 0.4 M sucrose for 20 min, and then dehydrated in the following plant vitrification solutions: PVS2 (13.7% sucrose, 30% glycerol, 15% ethylene glycol, 15% DMSO), PVS A3 (22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30, 40 and 50 min at 0 °C and PVS3 (50% glycerol and 50% sucrose) for 40, 50 and 60 min at room temperature. Explants dehydrated with PVS2 and PVS A3 were cryopreserved by vitrification while those dehydrated with PVS A3 and PVS3 were cryopreserved using droplet vitrification. In vitrification protocol, regrowth of the cryopreserved shoot tips dehydrated with PVS2 ranged between 20–40%. Dehydration with PVS A3 resulted in considerably higher regrowth rates (15–75%) using the same protocol. The highest values of regrowth were achieved with the longest treatment duration (50 min) for both vitrification solutions. As for droplet vitrification, regrowth of cryopreserved explants dehydrated with PVS A3 varied between 45–70%, and between 45–50% for those dehydrated with PVS3. The highest regrowth values, 70% and 50%, were achieved after 40-min PVS A3 treatment and 50-min PVS3 treatment, respectively. These results prove the feasibility of the PVS A3-based vitrification for the long-term storage of this genotype.",
publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade : University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts : 3rd International Conference on Plant Biology and 22nd SPPS Meeting, 9-12 June 2018, Belgrade",
title = "Cryopreservation of apple",
pages = "97-97"
}

Vujović, T., Ružić, Đ., Marjanović, T.,& Cerović, R.. (2018). Cryopreservation of apple. in Book of Abstracts : 3rd International Conference on Plant Biology and 22nd SPPS Meeting, 9-12 June 2018, Belgrade
Belgrade : Serbian Plant Physiology Society., 97-97.

Vujović T, Ružić Đ, Marjanović T, Cerović R. Cryopreservation of apple. in Book of Abstracts : 3rd International Conference on Plant Biology and 22nd SPPS Meeting, 9-12 June 2018, Belgrade. 2018;:97-97..

Vujović, Tatjana, Ružić, Đurđina, Marjanović, Tatjana, Cerović, Radosav, "Cryopreservation of apple" in Book of Abstracts : 3rd International Conference on Plant Biology and 22nd SPPS Meeting, 9-12 June 2018, Belgrade (2018):97-97.

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
AU  - Leposavić, Aleksandar
AU  - Karaklajić-Stajić, Žaklina
AU  - Mitrović, Olga
AU  - Zurawicz, Edward
PY  - 2017
UR  - https://refri.institut-cacak.org/handle/123456789/377
AB  - In vitro clonal propagation of small fruit species has been one of the most broadly exploited approaches in biotechnology. However, the most critical aspect of this propagation technology has been the maintenance of genetic fidelity of tissue culture-raised plants. In the present study, three techniques were deployed in the assessment of genetic fidelity of micropropagated plants (TC plants) of blackberry cultivar Cacanska Bestrna' and raspberry cultivar 'Meeker' in the second year after planting in the open field, in comparison to plants originating from the standard planting material (ST plants). Flow cytometry analysis deployed to estimate the DNA ploidy level and relative nuclear DNA content in TC and ST plants revealed no significant differences in nuclear DNA content between plants of different origin. Chromosome counting in root/shoot tip meristems also showed a normal tetraploid chromosome number (2n = 4x = 28) in TC blackberry plants and a normal diploid chromosome number (2n = 2x = 14) in TC raspberry plants. Polyacrylamide gel electrophoresis of peroxidase (POX) extracted from the leaf tissue revealed no differences in POX profiles between TC and ST plants either. The results obtained in this study verify the suitability of micropropagation by axillary branching for commercial exploitation in blackberry and raspberry.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - An assessment of the genetic integrity of micropropagated raspberry and blackberry plants
EP  - 461
SP  - 454
VL  - 225
DO  - 10.1016/j.scienta.2017.07.020
UR  - conv_1570
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav and Leposavić, Aleksandar and Karaklajić-Stajić, Žaklina and Mitrović, Olga and Zurawicz, Edward",
year = "2017",
abstract = "In vitro clonal propagation of small fruit species has been one of the most broadly exploited approaches in biotechnology. However, the most critical aspect of this propagation technology has been the maintenance of genetic fidelity of tissue culture-raised plants. In the present study, three techniques were deployed in the assessment of genetic fidelity of micropropagated plants (TC plants) of blackberry cultivar Cacanska Bestrna' and raspberry cultivar 'Meeker' in the second year after planting in the open field, in comparison to plants originating from the standard planting material (ST plants). Flow cytometry analysis deployed to estimate the DNA ploidy level and relative nuclear DNA content in TC and ST plants revealed no significant differences in nuclear DNA content between plants of different origin. Chromosome counting in root/shoot tip meristems also showed a normal tetraploid chromosome number (2n = 4x = 28) in TC blackberry plants and a normal diploid chromosome number (2n = 2x = 14) in TC raspberry plants. Polyacrylamide gel electrophoresis of peroxidase (POX) extracted from the leaf tissue revealed no differences in POX profiles between TC and ST plants either. The results obtained in this study verify the suitability of micropropagation by axillary branching for commercial exploitation in blackberry and raspberry.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "An assessment of the genetic integrity of micropropagated raspberry and blackberry plants",
pages = "461-454",
volume = "225",
doi = "10.1016/j.scienta.2017.07.020",
url = "conv_1570"
}

Vujović, T., Ružić, Đ., Cerović, R., Leposavić, A., Karaklajić-Stajić, Ž., Mitrović, O.,& Zurawicz, E.. (2017). An assessment of the genetic integrity of micropropagated raspberry and blackberry plants. in Scientia Horticulturae
Elsevier, Amsterdam., 225, 454-461.
https://doi.org/10.1016/j.scienta.2017.07.020
conv_1570

Vujović T, Ružić Đ, Cerović R, Leposavić A, Karaklajić-Stajić Ž, Mitrović O, Zurawicz E. An assessment of the genetic integrity of micropropagated raspberry and blackberry plants. in Scientia Horticulturae. 2017;225:454-461.
doi:10.1016/j.scienta.2017.07.020
conv_1570 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, Leposavić, Aleksandar, Karaklajić-Stajić, Žaklina, Mitrović, Olga, Zurawicz, Edward, "An assessment of the genetic integrity of micropropagated raspberry and blackberry plants" in Scientia Horticulturae, 225 (2017):454-461,
https://doi.org/10.1016/j.scienta.2017.07.020 .,
conv_1570 .

TY  - JOUR
AU  - Leposavić, Aleksandar
AU  - Ružić, Đurđina
AU  - Karaklajić-Stajić, Žaklina
AU  - Cerović, Radosav
AU  - Vujović, Tatjana
AU  - Zurawicz, Edward
AU  - Mitrović, Olga
PY  - 2016
UR  - https://refri.institut-cacak.org/handle/123456789/366
AB  - The objective of this study was to test yield and fruit quality of the red raspberry 'Meeker' and the blackberry 'Cacanska Bestrna' propagated using the standard method (ST) and in vitro, by tissue culture (TC). The monitored parameters included the total number of canes, cane number per one metre of the planted row, yield per cane (raspberry) or per bush (blackberry) and total yield. Monitoring fruit quality parameters included the weight and dimensions of fruit, the number, weight and dimensions of individual drupelets and weight of drupelet seeds. No significant differences were determined either for the total number of canes and/or the cane number per row metre in plants originating from both types of planting material in both genotypes. Significant differences were observed to the advantage of the raspberry ST plants, in the total yield, as well as the fruit weight in the blackberry ST plants. A significantly higher weight of drupelet seeds was observed in TC plants of both genotypes. Concerning the organoleptic assessment of fruits, no significant differences were recorded between fruits coming from ST and TC plants.
PB  - Wydawnictwo Akad Rolniczej W Lublinie
T2  - Acta Scientiarum Polonorum, Hortorum Cultus
T1  - Field performance of micropropagated rubus species
EP  - 14
IS  - 5
SP  - 3
VL  - 15
UR  - conv_1695
ER  - 

@article{
author = "Leposavić, Aleksandar and Ružić, Đurđina and Karaklajić-Stajić, Žaklina and Cerović, Radosav and Vujović, Tatjana and Zurawicz, Edward and Mitrović, Olga",
year = "2016",
abstract = "The objective of this study was to test yield and fruit quality of the red raspberry 'Meeker' and the blackberry 'Cacanska Bestrna' propagated using the standard method (ST) and in vitro, by tissue culture (TC). The monitored parameters included the total number of canes, cane number per one metre of the planted row, yield per cane (raspberry) or per bush (blackberry) and total yield. Monitoring fruit quality parameters included the weight and dimensions of fruit, the number, weight and dimensions of individual drupelets and weight of drupelet seeds. No significant differences were determined either for the total number of canes and/or the cane number per row metre in plants originating from both types of planting material in both genotypes. Significant differences were observed to the advantage of the raspberry ST plants, in the total yield, as well as the fruit weight in the blackberry ST plants. A significantly higher weight of drupelet seeds was observed in TC plants of both genotypes. Concerning the organoleptic assessment of fruits, no significant differences were recorded between fruits coming from ST and TC plants.",
publisher = "Wydawnictwo Akad Rolniczej W Lublinie",
journal = "Acta Scientiarum Polonorum, Hortorum Cultus",
title = "Field performance of micropropagated rubus species",
pages = "14-3",
number = "5",
volume = "15",
url = "conv_1695"
}

Leposavić, A., Ružić, Đ., Karaklajić-Stajić, Ž., Cerović, R., Vujović, T., Zurawicz, E.,& Mitrović, O.. (2016). Field performance of micropropagated rubus species. in Acta Scientiarum Polonorum, Hortorum Cultus
Wydawnictwo Akad Rolniczej W Lublinie., 15(5), 3-14.
conv_1695

Leposavić A, Ružić Đ, Karaklajić-Stajić Ž, Cerović R, Vujović T, Zurawicz E, Mitrović O. Field performance of micropropagated rubus species. in Acta Scientiarum Polonorum, Hortorum Cultus. 2016;15(5):3-14.
conv_1695 .

Leposavić, Aleksandar, Ružić, Đurđina, Karaklajić-Stajić, Žaklina, Cerović, Radosav, Vujović, Tatjana, Zurawicz, Edward, Mitrović, Olga, "Field performance of micropropagated rubus species" in Acta Scientiarum Polonorum, Hortorum Cultus, 15, no. 5 (2016):3-14,
conv_1695 .

TY  - CONF
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2016
UR  - https://refri.institut-cacak.org/handle/123456789/345
AB  - The most important aspect of successful micropropagation, among other in vitro factors, is to use the optimal types and concentrations of plant growth regulators. With the aim of optimization of in vitro multiplication of pear rootstock 'Pyrodwarf' the effects of different cytokinins including benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) have been studied at concentrations of 0.2, 0.4, 1, 2 and 3 mg L-1, singly and combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0.1, 0.5 and 1 mg L-1. Murashige and Skoog (1962) was the basic medium used in all combinations. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. The highest multiplication index was obtained on medium with 1 mg L-1 BA and 0.1 mg L-1 IBA, hovewer, the highest length of axial and lateral shoots was obtained on media also with 1 mg L-1 BA without and with 0.5 mg L-1 IBA, respectively. Generally, very poor multiplication was achieved on media with 2iP, KIN and TDZ whereas in many utilized combinations of low concentration of 2iP and KIN with IBA, and particularly in those with KIN rhyzogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of ` Pyrodwarf' rootstock is limited to BA. KIN and 2iP in smaller concentration could be used for regeneration of sturdy and long shoots, especially for elongation phase, but also for rooting phase.
PB  - International Society for Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - In vitro multiplication of semi-dwarfing pear rootstock 'Pyrodwarf' in relation to cytokinin types
EP  - 284
SP  - 279
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.49
UR  - conv_1552
ER  - 

@conference{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2016",
abstract = "The most important aspect of successful micropropagation, among other in vitro factors, is to use the optimal types and concentrations of plant growth regulators. With the aim of optimization of in vitro multiplication of pear rootstock 'Pyrodwarf' the effects of different cytokinins including benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) have been studied at concentrations of 0.2, 0.4, 1, 2 and 3 mg L-1, singly and combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0.1, 0.5 and 1 mg L-1. Murashige and Skoog (1962) was the basic medium used in all combinations. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. The highest multiplication index was obtained on medium with 1 mg L-1 BA and 0.1 mg L-1 IBA, hovewer, the highest length of axial and lateral shoots was obtained on media also with 1 mg L-1 BA without and with 0.5 mg L-1 IBA, respectively. Generally, very poor multiplication was achieved on media with 2iP, KIN and TDZ whereas in many utilized combinations of low concentration of 2iP and KIN with IBA, and particularly in those with KIN rhyzogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of ` Pyrodwarf' rootstock is limited to BA. KIN and 2iP in smaller concentration could be used for regeneration of sturdy and long shoots, especially for elongation phase, but also for rooting phase.",
publisher = "International Society for Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "In vitro multiplication of semi-dwarfing pear rootstock 'Pyrodwarf' in relation to cytokinin types",
pages = "284-279",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.49",
url = "conv_1552"
}

Ružić, Đ., Vujović, T.,& Cerović, R.. (2016). In vitro multiplication of semi-dwarfing pear rootstock 'Pyrodwarf' in relation to cytokinin types. in III Balkan Symposium on Fruit Growing
International Society for Horticultural Science, Leuven 1., 1139, 279-284.
https://doi.org/10.17660/ActaHortic.2016.1139.49
conv_1552

Ružić Đ, Vujović T, Cerović R. In vitro multiplication of semi-dwarfing pear rootstock 'Pyrodwarf' in relation to cytokinin types. in III Balkan Symposium on Fruit Growing. 2016;1139:279-284.
doi:10.17660/ActaHortic.2016.1139.49
conv_1552 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "In vitro multiplication of semi-dwarfing pear rootstock 'Pyrodwarf' in relation to cytokinin types" in III Balkan Symposium on Fruit Growing, 1139 (2016):279-284,
https://doi.org/10.17660/ActaHortic.2016.1139.49 .,
conv_1552 .

TY  - CONF
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
AU  - Đorđević, Milena
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/335
AB  - We studied the capacity of jasmonic acid (JA) to improve the in vitro rooting phase in micropropagated shoots of very popular low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6, respectively. The experiment was performed during the rooting phase and it included 7 medium types containing Murashige and Skoog (1962) macro-and micro-salts reduced to half, organic complex unchanged and supplemented with JA at four concentrations -0.2, 0.5, 1 and 2 mg/L. The trial also involved two independent, standard rooting media supplemented with 1 mg/L of indole-3-butyric acid (IBA) and 1 mg/L of alpha-napthyl acetic acid (NAA), both combined with 0.1 mg/L gibberellic acid (GA3). The hormone-free medium (HF) served as a control. The rooting parameters: rooting rate (%), number and length of roots per rooted plant and quality of rooted plants were monitored in the study. The highest rooting rate in both genotypes was obtained on medium with the lowest JA concentration, i.e., 0.2 mg/L (93.3% Pyrodwarf and 40% Gisela 6), this medium also gave the greatest number of roots. Higher JA concentrations (0.5 and 1 mg/L) produced increment in root length. However, rooted plants were grown for longer time period on media supplemented with IBA or NAA. JA applied at 2 mg/L inhibited roots formation in both genotypes. Hormone-free medium had only a marginal effect on rooting in Pyrodwarf (16.7%) where roots were exceptionally long (4.6 cm in average), and had no effect on rooting in Gisela 6 genotype. The obtained results suggest that lower concentrations of JA should be used to improve the rooting process as they ensure a good root system and vigorous, high quality plantlets which, most importantly, are easier to acclimatize.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - Potential Application of Jasmonic Acid in In Vitro Rooting of Low Vigorous Pear and Cherry Rootstocks
EP  - 900
SP  - 895
VL  - 1099
DO  - 10.17660/ActaHortic.2015.1099.114
UR  - conv_1696
ER  - 

@conference{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav and Đorđević, Milena",
year = "2015",
abstract = "We studied the capacity of jasmonic acid (JA) to improve the in vitro rooting phase in micropropagated shoots of very popular low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6, respectively. The experiment was performed during the rooting phase and it included 7 medium types containing Murashige and Skoog (1962) macro-and micro-salts reduced to half, organic complex unchanged and supplemented with JA at four concentrations -0.2, 0.5, 1 and 2 mg/L. The trial also involved two independent, standard rooting media supplemented with 1 mg/L of indole-3-butyric acid (IBA) and 1 mg/L of alpha-napthyl acetic acid (NAA), both combined with 0.1 mg/L gibberellic acid (GA3). The hormone-free medium (HF) served as a control. The rooting parameters: rooting rate (%), number and length of roots per rooted plant and quality of rooted plants were monitored in the study. The highest rooting rate in both genotypes was obtained on medium with the lowest JA concentration, i.e., 0.2 mg/L (93.3% Pyrodwarf and 40% Gisela 6), this medium also gave the greatest number of roots. Higher JA concentrations (0.5 and 1 mg/L) produced increment in root length. However, rooted plants were grown for longer time period on media supplemented with IBA or NAA. JA applied at 2 mg/L inhibited roots formation in both genotypes. Hormone-free medium had only a marginal effect on rooting in Pyrodwarf (16.7%) where roots were exceptionally long (4.6 cm in average), and had no effect on rooting in Gisela 6 genotype. The obtained results suggest that lower concentrations of JA should be used to improve the rooting process as they ensure a good root system and vigorous, high quality plantlets which, most importantly, are easier to acclimatize.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Potential Application of Jasmonic Acid in In Vitro Rooting of Low Vigorous Pear and Cherry Rootstocks",
pages = "900-895",
volume = "1099",
doi = "10.17660/ActaHortic.2015.1099.114",
url = "conv_1696"
}

Ružić, Đ., Vujović, T., Cerović, R.,& Đorđević, M.. (2015). Potential Application of Jasmonic Acid in In Vitro Rooting of Low Vigorous Pear and Cherry Rootstocks. in Acta Horticulturae
International Society for Horticultural Science., 1099, 895-900.
https://doi.org/10.17660/ActaHortic.2015.1099.114
conv_1696

Ružić Đ, Vujović T, Cerović R, Đorđević M. Potential Application of Jasmonic Acid in In Vitro Rooting of Low Vigorous Pear and Cherry Rootstocks. in Acta Horticulturae. 2015;1099:895-900.
doi:10.17660/ActaHortic.2015.1099.114
conv_1696 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, Đorđević, Milena, "Potential Application of Jasmonic Acid in In Vitro Rooting of Low Vigorous Pear and Cherry Rootstocks" in Acta Horticulturae, 1099 (2015):895-900,
https://doi.org/10.17660/ActaHortic.2015.1099.114 .,
conv_1696 .

TY  - CONF
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/332
AB  - The droplet vitrification technique was adapted to blackberry 'Cacanska Bestrna' (Rubus fruticosus L.) by applying two different plant vitrification solutions as well as by optimizing the duration of vitrification treatments. Apical shoot tips were pretreated in liquid Murashige and Skoog (MS) medium with progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in solution containing 1.9 M glycerol and 0.5 M sucrose for 30 min. After loading, explants were dehydrated for 20, 30 and 40 min on ice with vitrification solution A3 (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40, 50 and 60 min at room temperature with PVS3 solution (glycerol 50% and sucrose 50%). Explants were frozen in individual microdroplets of vitrification solution by direct immersion in liquid nitrogen. After rapid rewarming performed by direct immersion in unloading solution containing 0.8 M sucrose for 30 min, explants were placed onto solid MS medium supplemented with 1 mg/L N-6-benzyladenine and 0.1 mg/L indole-3-butyric acid, cultivated in the dark for 7 days and than transferred to standard growth conditions. Under the described experimental conditions, survival and regrowth of cryopreserved shoot tips dehydrated on ice with A3 solution ranged between 45.0-90.9 and 25.0-61.8%, respectively. Longer exposure of explants to A3 solution brought about higher survival and regrowth rates. On the other hand, dehydration with PVS3 resulted in considerably higher survival rates (90.0-95.9%) and higher regrowth rates (77.3-90.0%) of cryopreserved explants. The shortest PVS3 treatment gave the highest survival and regrowth. The results obtained prove the feasibility of PVS3-based droplet vitrification technique for long-term storage of this genotype, while further research will focus on evaluation of the optimized protocol for its applicability to different genotypes of the Rubus genus.
PB  - International Society for Horticultural Science, Leuven 1
C3  - II International Symposium on Horticulture In Europe
T1  - Optimization of Droplet Vitrification Protocol for Cryopreservation of In Vitro Grown Blackberry Shoot Tips
EP  - 601
SP  - 595
VL  - 1099
DO  - 10.17660/ActaHortic.2015.1099.72
UR  - conv_1548
ER  - 

@conference{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav",
year = "2015",
abstract = "The droplet vitrification technique was adapted to blackberry 'Cacanska Bestrna' (Rubus fruticosus L.) by applying two different plant vitrification solutions as well as by optimizing the duration of vitrification treatments. Apical shoot tips were pretreated in liquid Murashige and Skoog (MS) medium with progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in solution containing 1.9 M glycerol and 0.5 M sucrose for 30 min. After loading, explants were dehydrated for 20, 30 and 40 min on ice with vitrification solution A3 (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40, 50 and 60 min at room temperature with PVS3 solution (glycerol 50% and sucrose 50%). Explants were frozen in individual microdroplets of vitrification solution by direct immersion in liquid nitrogen. After rapid rewarming performed by direct immersion in unloading solution containing 0.8 M sucrose for 30 min, explants were placed onto solid MS medium supplemented with 1 mg/L N-6-benzyladenine and 0.1 mg/L indole-3-butyric acid, cultivated in the dark for 7 days and than transferred to standard growth conditions. Under the described experimental conditions, survival and regrowth of cryopreserved shoot tips dehydrated on ice with A3 solution ranged between 45.0-90.9 and 25.0-61.8%, respectively. Longer exposure of explants to A3 solution brought about higher survival and regrowth rates. On the other hand, dehydration with PVS3 resulted in considerably higher survival rates (90.0-95.9%) and higher regrowth rates (77.3-90.0%) of cryopreserved explants. The shortest PVS3 treatment gave the highest survival and regrowth. The results obtained prove the feasibility of PVS3-based droplet vitrification technique for long-term storage of this genotype, while further research will focus on evaluation of the optimized protocol for its applicability to different genotypes of the Rubus genus.",
publisher = "International Society for Horticultural Science, Leuven 1",
journal = "II International Symposium on Horticulture In Europe",
title = "Optimization of Droplet Vitrification Protocol for Cryopreservation of In Vitro Grown Blackberry Shoot Tips",
pages = "601-595",
volume = "1099",
doi = "10.17660/ActaHortic.2015.1099.72",
url = "conv_1548"
}

Vujović, T., Ružić, Đ.,& Cerović, R.. (2015). Optimization of Droplet Vitrification Protocol for Cryopreservation of In Vitro Grown Blackberry Shoot Tips. in II International Symposium on Horticulture In Europe
International Society for Horticultural Science, Leuven 1., 1099, 595-601.
https://doi.org/10.17660/ActaHortic.2015.1099.72
conv_1548

Vujović T, Ružić Đ, Cerović R. Optimization of Droplet Vitrification Protocol for Cryopreservation of In Vitro Grown Blackberry Shoot Tips. in II International Symposium on Horticulture In Europe. 2015;1099:595-601.
doi:10.17660/ActaHortic.2015.1099.72
conv_1548 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Optimization of Droplet Vitrification Protocol for Cryopreservation of In Vitro Grown Blackberry Shoot Tips" in II International Symposium on Horticulture In Europe, 1099 (2015):595-601,
https://doi.org/10.17660/ActaHortic.2015.1099.72 .,
conv_1548 .

TY  - CONF
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/331
AB  - Protocols for in vitro conservation of cherry plum (Prunus cerasifera Ehrh.) have been developed. In vitro grown shoot tips were tested for regrowth after conservation using encapsulation dehydration (E-D) method described by Dereuddre et al. (1990) and 'Cold storage' (CS) techniques. For E-D osmotic dehydration in 0.75 M sucrose followed by 8-h desiccation gave the highest regrowth (60%) of explants encapsulated in 3 and 5% alginate beads. Cryopreserved shoot tips multiplied in the three subcultures had normal morphology. During CS, shoots were maintained at + 5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Three months after CS, shoots of cherry plum were viable but etiolated showing symptoms of leaf necrosis. However, after 6 and 9 months of CS shoots showed severe signs of necrosis. Our results have shown that in vitro propagated cherry plum can be preserved for a longer period by E-D technique, while only 3 months by CS.
PB  - International Society for Horticultural Science
C3  - Acta Horticulturae
T1  - In Vitro Conservation of Prunus cerasifera Ehrh. by Encapsulation Dehydration and 'Cold Storage' Techniques
EP  - 594
SP  - 587
VL  - 1099
DO  - 10.17660/ActaHortic.2015.1099.71
UR  - conv_1697
ER  - 

@conference{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2015",
abstract = "Protocols for in vitro conservation of cherry plum (Prunus cerasifera Ehrh.) have been developed. In vitro grown shoot tips were tested for regrowth after conservation using encapsulation dehydration (E-D) method described by Dereuddre et al. (1990) and 'Cold storage' (CS) techniques. For E-D osmotic dehydration in 0.75 M sucrose followed by 8-h desiccation gave the highest regrowth (60%) of explants encapsulated in 3 and 5% alginate beads. Cryopreserved shoot tips multiplied in the three subcultures had normal morphology. During CS, shoots were maintained at + 5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Three months after CS, shoots of cherry plum were viable but etiolated showing symptoms of leaf necrosis. However, after 6 and 9 months of CS shoots showed severe signs of necrosis. Our results have shown that in vitro propagated cherry plum can be preserved for a longer period by E-D technique, while only 3 months by CS.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "In Vitro Conservation of Prunus cerasifera Ehrh. by Encapsulation Dehydration and 'Cold Storage' Techniques",
pages = "594-587",
volume = "1099",
doi = "10.17660/ActaHortic.2015.1099.71",
url = "conv_1697"
}

Ružić, Đ., Vujović, T.,& Cerović, R.. (2015). In Vitro Conservation of Prunus cerasifera Ehrh. by Encapsulation Dehydration and 'Cold Storage' Techniques. in Acta Horticulturae
International Society for Horticultural Science., 1099, 587-594.
https://doi.org/10.17660/ActaHortic.2015.1099.71
conv_1697

Ružić Đ, Vujović T, Cerović R. In Vitro Conservation of Prunus cerasifera Ehrh. by Encapsulation Dehydration and 'Cold Storage' Techniques. in Acta Horticulturae. 2015;1099:587-594.
doi:10.17660/ActaHortic.2015.1099.71
conv_1697 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "In Vitro Conservation of Prunus cerasifera Ehrh. by Encapsulation Dehydration and 'Cold Storage' Techniques" in Acta Horticulturae, 1099 (2015):587-594,
https://doi.org/10.17660/ActaHortic.2015.1099.71 .,
conv_1697 .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/338
AB  - In vitro shoot tips of Serbian autochthonous plums 'Sitnica' (Prunus domestica L.) and 'Crvena Ranka' (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum 'Sitnica', with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. 'Crvena Ranka' exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3-81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.
PB  - Springer, New York
T2  - Biologia
T1  - Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification
EP  - 1365
IS  - 10
SP  - 1359
VL  - 70
DO  - 10.1515/biolog-2015-0162
UR  - conv_1537
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav",
year = "2015",
abstract = "In vitro shoot tips of Serbian autochthonous plums 'Sitnica' (Prunus domestica L.) and 'Crvena Ranka' (Prunus insititia L.) were tested for recovery after cryopreservation using the droplet-vitrification technique. After 30-min loading with 1.9 M glycerol and 0.5 M sucrose, explants were dehydrated at room temperature for 10, 20, 30, 40 and 50 min with PVS A3 solution (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or for 60, 90 and 120 min using PVS3 solution (50% glycerol and 50% sucrose). Rewarming was performed in unloading solution containing 0.8 M sucrose for 30 or 60 min. Duration of PVS3 treatment significantly affected survival (27.3-72.7%) and regrowth (0-18.2%) of cryopreserved explants in plum 'Sitnica', with the highest values of both parameters being achieved with the 90-min treatment. Also, survival of explants dehydrated with PVS A3 solution significantly varied between 18.2-73.9%, depending on duration of both dehydration and unloading treatments. However, cryopreserved explants displayed a very low regrowth capacity, the highest being 10% for 30-min dehydration in combination with 60-min unloading. 'Crvena Ranka' exhibited a higher regrowth capacity after cryopreservation. Duration of PVS3 treatment significantly affected survival (22.2-77.8%) and regrowth (0-30.0%) of cryopreserved explants, with the highest values of both parameters being achieved with the shortest treatment duration. As regards PVS A3 treatments, both survival and regrowth significantly varied between 27.3-81.8%, and 0-36.4%, respectively. The highest regrowth was achieved with 20- and 30-min treatment durations combined with 30-min unloading. Further optimization of the protocol is necessary to improve recovery after cryopreservation.",
publisher = "Springer, New York",
journal = "Biologia",
title = "Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification",
pages = "1365-1359",
number = "10",
volume = "70",
doi = "10.1515/biolog-2015-0162",
url = "conv_1537"
}

Vujović, T., Ružić, Đ.,& Cerović, R.. (2015). Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification. in Biologia
Springer, New York., 70(10), 1359-1365.
https://doi.org/10.1515/biolog-2015-0162
conv_1537

Vujović T, Ružić Đ, Cerović R. Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification. in Biologia. 2015;70(10):1359-1365.
doi:10.1515/biolog-2015-0162
conv_1537 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Cryopreservation of Serbian autochthonous Prunus spp. by droplet-vitrification" in Biologia, 70, no. 10 (2015):1359-1365,
https://doi.org/10.1515/biolog-2015-0162 .,
conv_1537 .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Chatelet, Philippe
AU  - Ružić, Đurđina
AU  - Engelmann, Florent
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/339
AB  - Cryopreservation using aluminium cryo-plates was successfully applied to in vitro-grown shoot tips of two Prunus genotypes, cherry plum (Prunus cerasifera Ehrh.) and the plum cultivar 'Pozegaca' (Prunus domestica L.). Shoot tips were dissected from the shoots and precultured for 1 day at 23 degrees C in the dark on Murashige and Skoog medium containing 0.3 M sucrose. The precultured shoot tips were placed on aluminium cryo-plates containing 10 or 12 wells and embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates in two types of loading solution (LS1 - 2 M glycerol + 0.4 M sucrose or C4 - 1.9 M glycerol + 0.5 M sucrose) for 30 min at room temperature. In the V cryo-plate protocol, dehydration was performed for 30 min at room temperature in a plant vitrification solution containing 37.5% (w/v) glycerol, 15% (w/v) dimethylsulfoxide, 15% (w/v) ethylene glycol and 22.5% (w/v) sucrose. In the D cryo-plate protocol, dehydration was performed by placing the cryo-plates for 2, 2.5 or 3 h under the air current of the laminar flow cabinet or in closed glass containers over silica gel. In both protocols, cooling was performed by placing the cryo-plates in uncapped 2 mL cryotubes, which were immersed in liquid nitrogen. Rewarming was done by direct plunging of cryo-plates in liquid MS medium containing 0.8 M sucrose at room temperature for 30 min (plum cultivar) or 60 min (cherry plum). All experiments were conducted twice in two different laboratories (IRD Montpellier, France and FRI Cgak, Serbia). In the V cryo-plate procedure regrowth (calculated as average values for two laboratories) of cryopreserved shoot tips loaded with C4 solution was 41.7% (cherry plum) and 34.2% (plum cultivar 'Pogegga'), while in those loaded with LS1 solution regrowth was 56.1% and 44.6%, respectively. As for the D cryo-plate procedure, the average regrowth of cryopreserved explants ranged between 57.7% and 77.5% in cherry plum and between 28.5% and 47.5% in plum cultivar 'Pozegaca'. Although during the first subculture after regrowth, the multiplication capacity of cryopreserved explants was lower compared with those originating from dissection controls, by the third subculture they regained and even exceeded the multiplication capacity of shoots regenerated from explants placed on regrowth directly after dissection. The results obtained clearly indicate that both cryopreservation procedures using aluminium cryo-plates can facilitate efficient cryostorage of Prunus germplasm.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Cryopreservation of Prunus spp. using aluminium cryo-plates
EP  - 182
SP  - 173
VL  - 195
DO  - 10.1016/j.scienta.2015.09.016
UR  - conv_1536
ER  - 

@article{
author = "Vujović, Tatjana and Chatelet, Philippe and Ružić, Đurđina and Engelmann, Florent",
year = "2015",
abstract = "Cryopreservation using aluminium cryo-plates was successfully applied to in vitro-grown shoot tips of two Prunus genotypes, cherry plum (Prunus cerasifera Ehrh.) and the plum cultivar 'Pozegaca' (Prunus domestica L.). Shoot tips were dissected from the shoots and precultured for 1 day at 23 degrees C in the dark on Murashige and Skoog medium containing 0.3 M sucrose. The precultured shoot tips were placed on aluminium cryo-plates containing 10 or 12 wells and embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates in two types of loading solution (LS1 - 2 M glycerol + 0.4 M sucrose or C4 - 1.9 M glycerol + 0.5 M sucrose) for 30 min at room temperature. In the V cryo-plate protocol, dehydration was performed for 30 min at room temperature in a plant vitrification solution containing 37.5% (w/v) glycerol, 15% (w/v) dimethylsulfoxide, 15% (w/v) ethylene glycol and 22.5% (w/v) sucrose. In the D cryo-plate protocol, dehydration was performed by placing the cryo-plates for 2, 2.5 or 3 h under the air current of the laminar flow cabinet or in closed glass containers over silica gel. In both protocols, cooling was performed by placing the cryo-plates in uncapped 2 mL cryotubes, which were immersed in liquid nitrogen. Rewarming was done by direct plunging of cryo-plates in liquid MS medium containing 0.8 M sucrose at room temperature for 30 min (plum cultivar) or 60 min (cherry plum). All experiments were conducted twice in two different laboratories (IRD Montpellier, France and FRI Cgak, Serbia). In the V cryo-plate procedure regrowth (calculated as average values for two laboratories) of cryopreserved shoot tips loaded with C4 solution was 41.7% (cherry plum) and 34.2% (plum cultivar 'Pogegga'), while in those loaded with LS1 solution regrowth was 56.1% and 44.6%, respectively. As for the D cryo-plate procedure, the average regrowth of cryopreserved explants ranged between 57.7% and 77.5% in cherry plum and between 28.5% and 47.5% in plum cultivar 'Pozegaca'. Although during the first subculture after regrowth, the multiplication capacity of cryopreserved explants was lower compared with those originating from dissection controls, by the third subculture they regained and even exceeded the multiplication capacity of shoots regenerated from explants placed on regrowth directly after dissection. The results obtained clearly indicate that both cryopreservation procedures using aluminium cryo-plates can facilitate efficient cryostorage of Prunus germplasm.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Cryopreservation of Prunus spp. using aluminium cryo-plates",
pages = "182-173",
volume = "195",
doi = "10.1016/j.scienta.2015.09.016",
url = "conv_1536"
}

Vujović, T., Chatelet, P., Ružić, Đ.,& Engelmann, F.. (2015). Cryopreservation of Prunus spp. using aluminium cryo-plates. in Scientia Horticulturae
Elsevier, Amsterdam., 195, 173-182.
https://doi.org/10.1016/j.scienta.2015.09.016
conv_1536

Vujović T, Chatelet P, Ružić Đ, Engelmann F. Cryopreservation of Prunus spp. using aluminium cryo-plates. in Scientia Horticulturae. 2015;195:173-182.
doi:10.1016/j.scienta.2015.09.016
conv_1536 .

Vujović, Tatjana, Chatelet, Philippe, Ružić, Đurđina, Engelmann, Florent, "Cryopreservation of Prunus spp. using aluminium cryo-plates" in Scientia Horticulturae, 195 (2015):173-182,
https://doi.org/10.1016/j.scienta.2015.09.016 .,
conv_1536 .

TY  - JOUR
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2015
UR  - https://refri.institut-cacak.org/handle/123456789/340
AB  - The paper presents results of the application of 'Cold Storage' (CS), a very simple in vitro technique preservation of cultures at +5 degrees C in total darkness. A protocol has been developed for in vitro preservation of cherry rootstock Gisela 5 based on this method. Upon the establishment of aseptic culture, the studied genotype was propagated in vitro on MS medium supplemented with BA at a concentration of 3.37 mg/L. During CS, in vitro shoots were maintained at +5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after their respective period of time, the shoots were examined for viability for further propagation, together with their multiplication index and length of axial and lateral shoots. Three months after CS, shoots showed high shoot viability (55%), which however declined considerably after 6 and 9 months (15% and 0%, resp.). After 9 months of preservation under cold conditions, shoots showed severe signs of necrosis (55%). The transfer of cultures from the cold chamber to standard growth conditions led to prompt development and greening of leaves which regained morphology and capacity for multiplication and rooting.
PB  - National Institute of Science Communication and Information Resources
T2  - Indian Journal of Traditional Knowledge
T1  - In vitro conservation of cherry rootstock Gisela 5
EP  - 197
IS  - 2
SP  - 191
VL  - 14
UR  - conv_1699
ER  - 

@article{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2015",
abstract = "The paper presents results of the application of 'Cold Storage' (CS), a very simple in vitro technique preservation of cultures at +5 degrees C in total darkness. A protocol has been developed for in vitro preservation of cherry rootstock Gisela 5 based on this method. Upon the establishment of aseptic culture, the studied genotype was propagated in vitro on MS medium supplemented with BA at a concentration of 3.37 mg/L. During CS, in vitro shoots were maintained at +5 degrees C in cold chamber for 3, 6 and 9 months in total darkness. Seven days after their respective period of time, the shoots were examined for viability for further propagation, together with their multiplication index and length of axial and lateral shoots. Three months after CS, shoots showed high shoot viability (55%), which however declined considerably after 6 and 9 months (15% and 0%, resp.). After 9 months of preservation under cold conditions, shoots showed severe signs of necrosis (55%). The transfer of cultures from the cold chamber to standard growth conditions led to prompt development and greening of leaves which regained morphology and capacity for multiplication and rooting.",
publisher = "National Institute of Science Communication and Information Resources",
journal = "Indian Journal of Traditional Knowledge",
title = "In vitro conservation of cherry rootstock Gisela 5",
pages = "197-191",
number = "2",
volume = "14",
url = "conv_1699"
}

Ružić, Đ., Vujović, T.,& Cerović, R.. (2015). In vitro conservation of cherry rootstock Gisela 5. in Indian Journal of Traditional Knowledge
National Institute of Science Communication and Information Resources., 14(2), 191-197.
conv_1699

Ružić Đ, Vujović T, Cerović R. In vitro conservation of cherry rootstock Gisela 5. in Indian Journal of Traditional Knowledge. 2015;14(2):191-197.
conv_1699 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "In vitro conservation of cherry rootstock Gisela 5" in Indian Journal of Traditional Knowledge, 14, no. 2 (2015):191-197,
conv_1699 .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
PY  - 2014
UR  - https://refri.institut-cacak.org/handle/123456789/311
AB  - The paper presents evaluation of the influence of 30 combinations of plant growth regulators (PGRs) on induction of adventitious organogenesis from leaves of in vitro propagated shoots in 5 genotypes: sour cherry 'Cacanski Rubin', cherry rootstock Gisela 5, pear rootstock Pyrodwarf, blackberry 'Cacanska Bestrna' and raspberry 'Meeker'. Blackberry 'Cacanska Bestrna' showed the highest regeneration capacity (regeneration was induced with 12 PGR combinations, the highest regeneration frequency 41.7%), followed by sour cherry 'Cacanski Rubin' (9 PGR combinations, max. regeneration frequency 20.8%) and rootstocks Pyrodwarf (6 PGR combinations, max. regeneration frequency 12.5%) and Gisela 5 (3 PGR combinations, max. regeneration frequency 8.3%). Raspberry 'Meeker' displayed the lowest regeneration ability (1 PGR combination, 8.3%). Regeneration was mostly indirect via intermediate callus formation. Consistency and quantity of the formed callus were primarily determined by type and concentration of applied auxins. Two main types of callus were observed: hard, compact calli with nodular structures on the surface and soft, friable ones. In most of the genotypes evaluated, friable calli were rarely formed, with the exception of regeneration media containing 2,4-D where the formation of large amount of soft, watery and white or yellowish callus was noticed. Indirect regeneration occurred through hard, compact callus at cross-sectional area of leaf blade, mid vein and petiole, while it was never observed on intact parts of leaves including leaf edges.
PB  - Editura Ars Docendi
T2  - Romanian Biotechnological Letters
T1  - Adventitious organogenesis via intermediate callus formation in representatives of Prunus, Pyrus and Rubus genera
EP  - 9309
IS  - 3
SP  - 9297
VL  - 19
UR  - conv_1674
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav",
year = "2014",
abstract = "The paper presents evaluation of the influence of 30 combinations of plant growth regulators (PGRs) on induction of adventitious organogenesis from leaves of in vitro propagated shoots in 5 genotypes: sour cherry 'Cacanski Rubin', cherry rootstock Gisela 5, pear rootstock Pyrodwarf, blackberry 'Cacanska Bestrna' and raspberry 'Meeker'. Blackberry 'Cacanska Bestrna' showed the highest regeneration capacity (regeneration was induced with 12 PGR combinations, the highest regeneration frequency 41.7%), followed by sour cherry 'Cacanski Rubin' (9 PGR combinations, max. regeneration frequency 20.8%) and rootstocks Pyrodwarf (6 PGR combinations, max. regeneration frequency 12.5%) and Gisela 5 (3 PGR combinations, max. regeneration frequency 8.3%). Raspberry 'Meeker' displayed the lowest regeneration ability (1 PGR combination, 8.3%). Regeneration was mostly indirect via intermediate callus formation. Consistency and quantity of the formed callus were primarily determined by type and concentration of applied auxins. Two main types of callus were observed: hard, compact calli with nodular structures on the surface and soft, friable ones. In most of the genotypes evaluated, friable calli were rarely formed, with the exception of regeneration media containing 2,4-D where the formation of large amount of soft, watery and white or yellowish callus was noticed. Indirect regeneration occurred through hard, compact callus at cross-sectional area of leaf blade, mid vein and petiole, while it was never observed on intact parts of leaves including leaf edges.",
publisher = "Editura Ars Docendi",
journal = "Romanian Biotechnological Letters",
title = "Adventitious organogenesis via intermediate callus formation in representatives of Prunus, Pyrus and Rubus genera",
pages = "9309-9297",
number = "3",
volume = "19",
url = "conv_1674"
}

Vujović, T., Ružić, Đ.,& Cerović, R.. (2014). Adventitious organogenesis via intermediate callus formation in representatives of Prunus, Pyrus and Rubus genera. in Romanian Biotechnological Letters
Editura Ars Docendi., 19(3), 9297-9309.
conv_1674

Vujović T, Ružić Đ, Cerović R. Adventitious organogenesis via intermediate callus formation in representatives of Prunus, Pyrus and Rubus genera. in Romanian Biotechnological Letters. 2014;19(3):9297-9309.
conv_1674 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Adventitious organogenesis via intermediate callus formation in representatives of Prunus, Pyrus and Rubus genera" in Romanian Biotechnological Letters, 19, no. 3 (2014):9297-9309,
conv_1674 .

TY  - JOUR
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2014
UR  - https://refri.institut-cacak.org/handle/123456789/326
AB  - In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Horticultural Science
T1  - Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure
EP  - 63
IS  - 2
SP  - 55
VL  - 41
DO  - 10.17221/234/2013-HORTSCI
UR  - conv_1517
ER  - 

@article{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2014",
abstract = "In vitro-grown shoot tips of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock were tested for regrowth after cryopreservation using vitrification technique. Explants were precultured in the dark at 23 degrees C, in a liquid MS medium with a progressively increasing sucrose concentration (0.3 M for 15 h, then 0.7 M for 5 h), and subsequently loaded in a solution containing 2 M glycerol and 0.4 M sucrose for 20 minutes. Shoot tips were dehydrated at 0 degrees C using either the original PVS2 or modified PVS2 solution (PVS A3 - 22.5% sucrose, 37.5% glycerol, 15% ethylene glycol and 15% DMSO) for 30,40 and 50 minutes. The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 36-54% and 8-17%, respectively. However, the dehydration with the PVS A3 solution resulted in considerably higher survival rates (81-92%), as well as higher regrowth rates (39-56%) after cryopreservation. These results prove the feasibility of the PVS A3-based vitrification technique for a long-term storage of this genotype.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Horticultural Science",
title = "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure",
pages = "63-55",
number = "2",
volume = "41",
doi = "10.17221/234/2013-HORTSCI",
url = "conv_1517"
}

Ružić, Đ., Vujović, T.,& Cerović, R.. (2014). Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science
Czech Academy Agricultural Sciences, Prague., 41(2), 55-63.
https://doi.org/10.17221/234/2013-HORTSCI
conv_1517

Ružić Đ, Vujović T, Cerović R. Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure. in Horticultural Science. 2014;41(2):55-63.
doi:10.17221/234/2013-HORTSCI
conv_1517 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "Cryopreservation of cherry rootstock Gisela 5 using vitrification procedure" in Horticultural Science, 41, no. 2 (2014):55-63,
https://doi.org/10.17221/234/2013-HORTSCI .,
conv_1517 .

TY  - JOUR
AU  - Ružić, Đurđina
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
PY  - 2013
UR  - https://refri.institut-cacak.org/handle/123456789/292
AB  - The capacity of Jasmonic acid (JA) to improve in vitro multiplication phase in micropropagated shoots of low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6 respectively, was studied. The experiment was performed during the multiplication phase and it included 23 media types containing Murashige and Skoog (1962) macro, micro salts and organic complex supplemented with JA at four concentrations (1, 2.3, 5 and 10 μM) alone and in combination with benzyl adenine - BA (4.4 μM), indole-3-butyric acid - IBA (1, 2.30, 2.46, 5 and 10 μM), a-napthyl acetic acid -NAA (1, 2.30, 2.68, 5, 10 μM), and indole-3-acetic acid - IAA (1, 2.30, 2.85, 5, 10 μM). The multiplication parameters, such as multiplication index, length of axial and lateral shoots, FW and DW of shoots as well as quality of multiplied shoots were monitored. The highest multiplication rate in both genotypes was obtained on medium with BA (4.4 μM) in combination with IBA (1 and 2.3 μM). The longest axial and lateral shoots were also obtained on media with BA in combination with JA or other three auxins used. The main characteristics of the JA effect (used alone) were big shoots, large green leaves, small quantity of firm, nodular callus as well as occurrence of rooting. The obtained results suggest that JA used alone should not be used to improve the multiplication process, but only in combination with BA and IBA or NAA for the high quality plantlets which is important for the rooting phase, to skip elongation phase.
PB  - Research Institute for Fruit Growing
T2  - Fruit Growing Research
T1  - Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică
T1  - Effect of jasmonic acid on in vitro multiplication of low vigorous Pear and cherry rootstocks
EP  - 112
IS  - 1
SP  - 106
VL  - 29
UR  - conv_1716
ER  - 

@article{
author = "Ružić, Đurđina and Vujović, Tatjana and Cerović, Radosav",
year = "2013",
abstract = "The capacity of Jasmonic acid (JA) to improve in vitro multiplication phase in micropropagated shoots of low vigorous pear and cherry rootstocks - Pyrodwarf and Gisela 6 respectively, was studied. The experiment was performed during the multiplication phase and it included 23 media types containing Murashige and Skoog (1962) macro, micro salts and organic complex supplemented with JA at four concentrations (1, 2.3, 5 and 10 μM) alone and in combination with benzyl adenine - BA (4.4 μM), indole-3-butyric acid - IBA (1, 2.30, 2.46, 5 and 10 μM), a-napthyl acetic acid -NAA (1, 2.30, 2.68, 5, 10 μM), and indole-3-acetic acid - IAA (1, 2.30, 2.85, 5, 10 μM). The multiplication parameters, such as multiplication index, length of axial and lateral shoots, FW and DW of shoots as well as quality of multiplied shoots were monitored. The highest multiplication rate in both genotypes was obtained on medium with BA (4.4 μM) in combination with IBA (1 and 2.3 μM). The longest axial and lateral shoots were also obtained on media with BA in combination with JA or other three auxins used. The main characteristics of the JA effect (used alone) were big shoots, large green leaves, small quantity of firm, nodular callus as well as occurrence of rooting. The obtained results suggest that JA used alone should not be used to improve the multiplication process, but only in combination with BA and IBA or NAA for the high quality plantlets which is important for the rooting phase, to skip elongation phase.",
publisher = "Research Institute for Fruit Growing",
journal = "Fruit Growing Research",
title = "Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică, Effect of jasmonic acid on in vitro multiplication of low vigorous Pear and cherry rootstocks",
pages = "112-106",
number = "1",
volume = "29",
url = "conv_1716"
}

Ružić, Đ., Vujović, T.,& Cerović, R.. (2013). Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică. in Fruit Growing Research
Research Institute for Fruit Growing., 29(1), 106-112.
conv_1716

Ružić Đ, Vujović T, Cerović R. Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică. in Fruit Growing Research. 2013;29(1):106-112.
conv_1716 .

Ružić, Đurđina, Vujović, Tatjana, Cerović, Radosav, "Efectul acidului jasmonic asupra multiplicării in vitro a portaltoilor de păr şi cireş de vigoare mică" in Fruit Growing Research, 29, no. 1 (2013):106-112,
conv_1716 .

TY  - JOUR
AU  - Ružić, Đurđina
AU  - Leposavić, Aleksandar
AU  - Cerović, Radosav
AU  - Karaklajić-Stajić, Žaklina
AU  - Vujović, Tatjana
AU  - Miletić, Nemanja
AU  - Zurawicz, Edward
PY  - 2013
UR  - https://refri.institut-cacak.org/handle/123456789/278
AB  - Ova istraživanja su finansirana sredstvima projekta EU FP7 KBBE - 2010 -4, No. 265942 (The sustainable improvement of European berry production, quality and nutritional value in changing environment: Strawberries, Currants, Blackberries, Blueberries and Raspberries - EUBERRY), sa ciljem da se utvrdi potencijal upotrebe mikropropagacije in vitro u masovnoj proizvodnji kupine i maline za dobijanje zdravog sadnog materijala, genetički stabilnog i sortno ispravnog. U ogledu su korišćene kupina cv Cačanska bestrna i malina cv Meeker, razmnožene mikropropagacijom in vitro (TC) i standardnim načinom (SP) posađene na otvorenom polju na površini od 1,8 ari. Praćeni su fenološki parametri (početak listanja; formiranje/razvoj cvetnih pupoljaka; početak, puno i kraj cvetanja; početak, puno i kraj zrenja; period zrenja i trajanje vegetacije), vegetativni potencijal i parametri prinosa uključujući ukupan broj izdanaka, broj izdanaka po dužnom metru, prinos po izdanku (kg), prinos po dužnom metru (kg), ukupan prinos (kg ha-1). Statistički značajne razlike nisu utvrđene za praćene fenološke paramentre i vegetativni potencijal. Međutim, prinos po izdanku, po dužnom metru i ukupan prinos su se statistički značajno razlikovali u korist SP biljaka kupine, a samo prinos po izdanku, takođe u korist SP biljaka maline.
AB  - The present studies, financed by the project titled EU FP7 KBBE - 2010 -4 265942 (The sustainable improvement of European berry production, quality and nutritional value in changing environment: Strawberries, Currants, Blackberries, Blueberries and Raspberries -EUBERRY), were conducted to establish potentials of in vitro micropropagation for massive blackberry and raspberry production, primarily aimed at obtaining healthy, genetically stable and true-to-type planting material. Blackberry ‘Cačanska Bestrna’ and raspberry ‘Meeker’ plants propagated by standard (SP) and in vitro micropropagation (TC) techniques and planted in open field (1.8 acres) were used in the trial. The following parameters were monitored: phenological parameters (leafing onset, flower-cluster development, flowering onset, fool blooming, end of flowering, ripening onset, full ripening, end of ripening, as well as period of fruit ripening and duration of growing period), vegetative potential and yield parameters, including total number of canes, cane number per row meter, yield per cane (kg), yield per row meter (kg), and total yield (kg ha-1). No significant differences were established for the monitored phenological parameters and vegetative potential. However, yield per cane, yield per row meter and total yield differed statistically in favor of blackberry propagated by standard technique, whereas statistical differences were significant only for yield per cane also in favor of SP propagated raspberry plants.
PB  - Naučno voćarsko društvo Srbije, Čačak
T2  - Voćarstvo
T1  - Fiziološke osobine maline i kupine razmnožene mikropropagacijom in vitro i standardnim načinom
T1  - Physiological properties of raspberry and blackberry propagated by in vitro micropropagation and standard techniques
EP  - 61
IS  - 181-182
SP  - 55
VL  - 47
UR  - conv_1332
ER  - 

@article{
author = "Ružić, Đurđina and Leposavić, Aleksandar and Cerović, Radosav and Karaklajić-Stajić, Žaklina and Vujović, Tatjana and Miletić, Nemanja and Zurawicz, Edward",
year = "2013",
abstract = "Ova istraživanja su finansirana sredstvima projekta EU FP7 KBBE - 2010 -4, No. 265942 (The sustainable improvement of European berry production, quality and nutritional value in changing environment: Strawberries, Currants, Blackberries, Blueberries and Raspberries - EUBERRY), sa ciljem da se utvrdi potencijal upotrebe mikropropagacije in vitro u masovnoj proizvodnji kupine i maline za dobijanje zdravog sadnog materijala, genetički stabilnog i sortno ispravnog. U ogledu su korišćene kupina cv Cačanska bestrna i malina cv Meeker, razmnožene mikropropagacijom in vitro (TC) i standardnim načinom (SP) posađene na otvorenom polju na površini od 1,8 ari. Praćeni su fenološki parametri (početak listanja; formiranje/razvoj cvetnih pupoljaka; početak, puno i kraj cvetanja; početak, puno i kraj zrenja; period zrenja i trajanje vegetacije), vegetativni potencijal i parametri prinosa uključujući ukupan broj izdanaka, broj izdanaka po dužnom metru, prinos po izdanku (kg), prinos po dužnom metru (kg), ukupan prinos (kg ha-1). Statistički značajne razlike nisu utvrđene za praćene fenološke paramentre i vegetativni potencijal. Međutim, prinos po izdanku, po dužnom metru i ukupan prinos su se statistički značajno razlikovali u korist SP biljaka kupine, a samo prinos po izdanku, takođe u korist SP biljaka maline., The present studies, financed by the project titled EU FP7 KBBE - 2010 -4 265942 (The sustainable improvement of European berry production, quality and nutritional value in changing environment: Strawberries, Currants, Blackberries, Blueberries and Raspberries -EUBERRY), were conducted to establish potentials of in vitro micropropagation for massive blackberry and raspberry production, primarily aimed at obtaining healthy, genetically stable and true-to-type planting material. Blackberry ‘Cačanska Bestrna’ and raspberry ‘Meeker’ plants propagated by standard (SP) and in vitro micropropagation (TC) techniques and planted in open field (1.8 acres) were used in the trial. The following parameters were monitored: phenological parameters (leafing onset, flower-cluster development, flowering onset, fool blooming, end of flowering, ripening onset, full ripening, end of ripening, as well as period of fruit ripening and duration of growing period), vegetative potential and yield parameters, including total number of canes, cane number per row meter, yield per cane (kg), yield per row meter (kg), and total yield (kg ha-1). No significant differences were established for the monitored phenological parameters and vegetative potential. However, yield per cane, yield per row meter and total yield differed statistically in favor of blackberry propagated by standard technique, whereas statistical differences were significant only for yield per cane also in favor of SP propagated raspberry plants.",
publisher = "Naučno voćarsko društvo Srbije, Čačak",
journal = "Voćarstvo",
title = "Fiziološke osobine maline i kupine razmnožene mikropropagacijom in vitro i standardnim načinom, Physiological properties of raspberry and blackberry propagated by in vitro micropropagation and standard techniques",
pages = "61-55",
number = "181-182",
volume = "47",
url = "conv_1332"
}

Ružić, Đ., Leposavić, A., Cerović, R., Karaklajić-Stajić, Ž., Vujović, T., Miletić, N.,& Zurawicz, E.. (2013). Fiziološke osobine maline i kupine razmnožene mikropropagacijom in vitro i standardnim načinom. in Voćarstvo
Naučno voćarsko društvo Srbije, Čačak., 47(181-182), 55-61.
conv_1332

Ružić Đ, Leposavić A, Cerović R, Karaklajić-Stajić Ž, Vujović T, Miletić N, Zurawicz E. Fiziološke osobine maline i kupine razmnožene mikropropagacijom in vitro i standardnim načinom. in Voćarstvo. 2013;47(181-182):55-61.
conv_1332 .

Ružić, Đurđina, Leposavić, Aleksandar, Cerović, Radosav, Karaklajić-Stajić, Žaklina, Vujović, Tatjana, Miletić, Nemanja, Zurawicz, Edward, "Fiziološke osobine maline i kupine razmnožene mikropropagacijom in vitro i standardnim načinom" in Voćarstvo, 47, no. 181-182 (2013):55-61,
conv_1332 .

TY  - JOUR
AU  - Vujović, Tatjana
AU  - Ružić, Đurđina
AU  - Cerović, Radosav
PY  - 2013
UR  - https://refri.institut-cacak.org/handle/123456789/297
AB  - U radu je prikazan protokol za mikropropagaciju sorte višnje Čačanski rubin (Prunus cerasus L.) stvorene u Institutu za voćarstvo u Čačku. U cilju razvijanja protokola za brzu i efikasnu mikropropagaciju ovog genotipa ispitana je promena regenerativne sposobnosti in vitro izdanaka tokom supkultivisanja, kao i uticaj promene vrste i koncentracije biljnih regulatora rastenja na fazu multiplikacije i ožiljavanja. Primenom standardne metode povrsinske sterilizacije početnih eksplantata uspostavljena je aseptična kultura i inicirane lisne rozete na Murashige i Skoog (1962) (MS) hranljivoj podlozi sa 2,0 mg l-1 BAP, 0,5 mg l-1 IBA i 0,1 mg l-1 GA3. Po uspostavljanju aseptične kulture izdanci su multiplicirani u pet uzastopnih supkultura na MS hranljivoj podlozi koja je sadržavala 0,5 mg l-1 BAP u kombinaciji sa po 0,1 mg l-1 IBA i GA3. Praceni su sledeći parametri: indeks multiplikacije, dužina osovinskog i bočnih izdanaka. U cilju optimizacije faze multiplikacije, u 5. supkulturi je ispitan uticaj promene koncentracije BAP i/ili vrste auksina (IBA ili NAA) na parametre multiplikacije, svežu i suvu masu izdanaka. Utvrđeno je da se supkultivisanjem smanjila regenerativna sposobnost izdanaka prikazana kroz indeks multiplikacije u odnosu na prvu supkulturu (3,11), sto je, međutim, bilo praćeno povećanjem rastenja izdanaka, tako da je najveća prosečna dužina osovinskog izdanka (1,22 cm) utvrđena u petoj supkulturi. Iako je povećanje koncentracije BAP sa 0,5 na 1,0 mg l-1 i promena vrste auksina (NAA umesto IBA) u petoj supkulturi dovelo do povećanja indeksa multiplikacije, zbog značajnog smanjenja dužine, kao i pojave hiperhidričnosti multiplicirani izdanaci nisu bili pogodni za dalje gajenje. Sorta Čačanski rubin je pokazala nizak kapacitet za ožiljavanje na hranljivoj podlozi bez biljnih regulatora rastenja, kao i na hranljivoj podlozi sa 1,0 mg l-1 IBA koji je najčešće korišćen auksin za ožiljavanje vrsta roda Prunus, dok na hranljivoj podlozi sa istom koncentracijom NAA nije uočeno ožiljavanje. Najveći procenat ožiljavanja (60,0%) i prosecan broj korenova po ožiljenoj biljci (6,3) postignut je gajenjem na hranljivoj podlozi bez biljnih regulatora rastenja uz pretretman uranjanja bazalnog dela izdanaka u rastvor NAA (500 mg l-1). U uslovima 'mist' izmaglice procenat aklimatizacije izdanaka ožiljenih in vitro (55,6%) je bio značajno veći u odnosu na neožiljene izdanke (14,8%), kojima je prethodila indukcija rizogeneze.
AB  - The paper describes the protocol for micropropagation of sour cherry 'Čačanski Rubin' (Prunus cerasus L.) bred at the Fruit Research Institute in Čačak. Aiming at developing efficient protocol for micropropagation of this genotype, the influence of repeated subculturing on proliferation capacity of in vitro shoots as well as the effect of change of type and concentration of plant growth regulators on multiplication and rooting phases were studied. With standard method of surface sterilization of initial explants, aseptic culture was established and leaf rosettes were initiated on Murashige and Skoog (1962) medium (MS) with 2.0 mg l-1 BAP, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3. After establishment of aseptic culture, regenerated shoots were multiplied in five successive subcultures on MS medium supplemented with 0.5 mg l-1 BAP in combination with IBA and GA3 each applied at 0.1 mg l-1. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. With aim to optimize multiplication stage and determine the optimal hormonal composition of multiplication medium, the influence of increase in BAP concentration and/or type of auxins (IBA or NAA), on multiplication index, fresh and dry weight of shoots was examined in fifth subculture after initiation. During multiplication on MS medium with constant hormonal composition, the decline in shoot formation capacity over repeated subcultures was observed, so as the highest value of multiplication index (3.11) was noticed in first subculture after initiation. However the subculturing improved the shoot elongation, and the highest value of shoots length, especially in axial shoots (1.22 cm), was observed in fifth subculture. Although increase in BAP concentration from 0.5 to 1.0 mg l-1 in combination with NAA instead of IBA in fifth subculture increased multiplication index, the shoots multiplied on this medium were not suitable for further growing due to the significant decrease in shoot length as well as to the appearance of hyperhydricity. Sour cherry 'Čačanski Rubin' displayed very low rooting ability on HF medium as well as on medium with 1.0 mg l-1 IBA which is the most common medium used for rooting of different species of Prunus genus, while on medium containing NAA at same concentration rhizogenesis was not observed. The highest rooting rate (60.0%) as well as the highest number of roots per shoot (6.3) in this genotype were achieved with 1-min dip treatment in NAA dissolved in sterile water (500 mg l-1) followed by growing on HF medium. The percentage of acclimatization under the 'mist' system in green house was significantly higher for shoots rooted in vitro (55.6%) than for unrooted shoots (14,8%).
PB  - Naučno voćarsko društvo Srbije, Čačak
T2  - Voćarstvo
T1  - Mikropropagacija sorte višnje Čačanski rubin (Prunus cerasus L.)
T1  - Micropropagation of sour cherry 'Čačanski Rubin' (Prunus cerasus L.)
EP  - 119
IS  - 183-184
SP  - 109
VL  - 47
UR  - conv_1336
ER  - 

@article{
author = "Vujović, Tatjana and Ružić, Đurđina and Cerović, Radosav",
year = "2013",
abstract = "U radu je prikazan protokol za mikropropagaciju sorte višnje Čačanski rubin (Prunus cerasus L.) stvorene u Institutu za voćarstvo u Čačku. U cilju razvijanja protokola za brzu i efikasnu mikropropagaciju ovog genotipa ispitana je promena regenerativne sposobnosti in vitro izdanaka tokom supkultivisanja, kao i uticaj promene vrste i koncentracije biljnih regulatora rastenja na fazu multiplikacije i ožiljavanja. Primenom standardne metode povrsinske sterilizacije početnih eksplantata uspostavljena je aseptična kultura i inicirane lisne rozete na Murashige i Skoog (1962) (MS) hranljivoj podlozi sa 2,0 mg l-1 BAP, 0,5 mg l-1 IBA i 0,1 mg l-1 GA3. Po uspostavljanju aseptične kulture izdanci su multiplicirani u pet uzastopnih supkultura na MS hranljivoj podlozi koja je sadržavala 0,5 mg l-1 BAP u kombinaciji sa po 0,1 mg l-1 IBA i GA3. Praceni su sledeći parametri: indeks multiplikacije, dužina osovinskog i bočnih izdanaka. U cilju optimizacije faze multiplikacije, u 5. supkulturi je ispitan uticaj promene koncentracije BAP i/ili vrste auksina (IBA ili NAA) na parametre multiplikacije, svežu i suvu masu izdanaka. Utvrđeno je da se supkultivisanjem smanjila regenerativna sposobnost izdanaka prikazana kroz indeks multiplikacije u odnosu na prvu supkulturu (3,11), sto je, međutim, bilo praćeno povećanjem rastenja izdanaka, tako da je najveća prosečna dužina osovinskog izdanka (1,22 cm) utvrđena u petoj supkulturi. Iako je povećanje koncentracije BAP sa 0,5 na 1,0 mg l-1 i promena vrste auksina (NAA umesto IBA) u petoj supkulturi dovelo do povećanja indeksa multiplikacije, zbog značajnog smanjenja dužine, kao i pojave hiperhidričnosti multiplicirani izdanaci nisu bili pogodni za dalje gajenje. Sorta Čačanski rubin je pokazala nizak kapacitet za ožiljavanje na hranljivoj podlozi bez biljnih regulatora rastenja, kao i na hranljivoj podlozi sa 1,0 mg l-1 IBA koji je najčešće korišćen auksin za ožiljavanje vrsta roda Prunus, dok na hranljivoj podlozi sa istom koncentracijom NAA nije uočeno ožiljavanje. Najveći procenat ožiljavanja (60,0%) i prosecan broj korenova po ožiljenoj biljci (6,3) postignut je gajenjem na hranljivoj podlozi bez biljnih regulatora rastenja uz pretretman uranjanja bazalnog dela izdanaka u rastvor NAA (500 mg l-1). U uslovima 'mist' izmaglice procenat aklimatizacije izdanaka ožiljenih in vitro (55,6%) je bio značajno veći u odnosu na neožiljene izdanke (14,8%), kojima je prethodila indukcija rizogeneze., The paper describes the protocol for micropropagation of sour cherry 'Čačanski Rubin' (Prunus cerasus L.) bred at the Fruit Research Institute in Čačak. Aiming at developing efficient protocol for micropropagation of this genotype, the influence of repeated subculturing on proliferation capacity of in vitro shoots as well as the effect of change of type and concentration of plant growth regulators on multiplication and rooting phases were studied. With standard method of surface sterilization of initial explants, aseptic culture was established and leaf rosettes were initiated on Murashige and Skoog (1962) medium (MS) with 2.0 mg l-1 BAP, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3. After establishment of aseptic culture, regenerated shoots were multiplied in five successive subcultures on MS medium supplemented with 0.5 mg l-1 BAP in combination with IBA and GA3 each applied at 0.1 mg l-1. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. With aim to optimize multiplication stage and determine the optimal hormonal composition of multiplication medium, the influence of increase in BAP concentration and/or type of auxins (IBA or NAA), on multiplication index, fresh and dry weight of shoots was examined in fifth subculture after initiation. During multiplication on MS medium with constant hormonal composition, the decline in shoot formation capacity over repeated subcultures was observed, so as the highest value of multiplication index (3.11) was noticed in first subculture after initiation. However the subculturing improved the shoot elongation, and the highest value of shoots length, especially in axial shoots (1.22 cm), was observed in fifth subculture. Although increase in BAP concentration from 0.5 to 1.0 mg l-1 in combination with NAA instead of IBA in fifth subculture increased multiplication index, the shoots multiplied on this medium were not suitable for further growing due to the significant decrease in shoot length as well as to the appearance of hyperhydricity. Sour cherry 'Čačanski Rubin' displayed very low rooting ability on HF medium as well as on medium with 1.0 mg l-1 IBA which is the most common medium used for rooting of different species of Prunus genus, while on medium containing NAA at same concentration rhizogenesis was not observed. The highest rooting rate (60.0%) as well as the highest number of roots per shoot (6.3) in this genotype were achieved with 1-min dip treatment in NAA dissolved in sterile water (500 mg l-1) followed by growing on HF medium. The percentage of acclimatization under the 'mist' system in green house was significantly higher for shoots rooted in vitro (55.6%) than for unrooted shoots (14,8%).",
publisher = "Naučno voćarsko društvo Srbije, Čačak",
journal = "Voćarstvo",
title = "Mikropropagacija sorte višnje Čačanski rubin (Prunus cerasus L.), Micropropagation of sour cherry 'Čačanski Rubin' (Prunus cerasus L.)",
pages = "119-109",
number = "183-184",
volume = "47",
url = "conv_1336"
}

Vujović, T., Ružić, Đ.,& Cerović, R.. (2013). Mikropropagacija sorte višnje Čačanski rubin (Prunus cerasus L.). in Voćarstvo
Naučno voćarsko društvo Srbije, Čačak., 47(183-184), 109-119.
conv_1336

Vujović T, Ružić Đ, Cerović R. Mikropropagacija sorte višnje Čačanski rubin (Prunus cerasus L.). in Voćarstvo. 2013;47(183-184):109-119.
conv_1336 .

Vujović, Tatjana, Ružić, Đurđina, Cerović, Radosav, "Mikropropagacija sorte višnje Čačanski rubin (Prunus cerasus L.)" in Voćarstvo, 47, no. 183-184 (2013):109-119,
conv_1336 .