Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia
Abstract
In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch's postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.
Keywords:
TEF1 / ITS / Daffodil / Bulb rotSource:
Journal of Plant Diseases and Protection, 2021, 128, 5, 1357-1361Publisher:
- Springer Heidelberg, Heidelberg
Funding / projects:
- Fruit Research Institute, Cacak, Serbia
DOI: 10.1007/s41348-021-00476-5
ISSN: 1861-3829
WoS: 000648200800002
Scopus: 2-s2.0-85105457893
Collections
Institution/Community
Institut za voćarstvoTY - JOUR AU - Vasić, Tanja AU - Jevremović, Darko AU - Krnjaja, Vesna AU - Živković, Sanja AU - Trkulja, Vojislav PY - 2021 UR - https://refri.institut-cacak.org/handle/123456789/465 AB - In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch's postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country. PB - Springer Heidelberg, Heidelberg T2 - Journal of Plant Diseases and Protection T1 - Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia EP - 1361 IS - 5 SP - 1357 VL - 128 DO - 10.1007/s41348-021-00476-5 UR - conv_1613 ER -
@article{ author = "Vasić, Tanja and Jevremović, Darko and Krnjaja, Vesna and Živković, Sanja and Trkulja, Vojislav", year = "2021", abstract = "In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch's postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.", publisher = "Springer Heidelberg, Heidelberg", journal = "Journal of Plant Diseases and Protection", title = "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia", pages = "1361-1357", number = "5", volume = "128", doi = "10.1007/s41348-021-00476-5", url = "conv_1613" }
Vasić, T., Jevremović, D., Krnjaja, V., Živković, S.,& Trkulja, V.. (2021). Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection Springer Heidelberg, Heidelberg., 128(5), 1357-1361. https://doi.org/10.1007/s41348-021-00476-5 conv_1613
Vasić T, Jevremović D, Krnjaja V, Živković S, Trkulja V. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia. in Journal of Plant Diseases and Protection. 2021;128(5):1357-1361. doi:10.1007/s41348-021-00476-5 conv_1613 .
Vasić, Tanja, Jevremović, Darko, Krnjaja, Vesna, Živković, Sanja, Trkulja, Vojislav, "Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet's daffodil (Narcissus poeticus L.) in Serbia" in Journal of Plant Diseases and Protection, 128, no. 5 (2021):1357-1361, https://doi.org/10.1007/s41348-021-00476-5 ., conv_1613 .