Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover
2016
Преузимање 🢃
Аутори
Vasić, TanjaKrnjaja, Vesna
Jevremović, Darko
Stanković, Slavica
Terzić, Dragan
Milenković, Jasmina
Marković, Jordan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geog...raphical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.
Кључне речи:
VCGs / T. pratense / RFLP / M. sativa / C. destructivumИзвор:
Genetika-Belgrade, 2016, 48, 1, 187-198Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Побољшање генетичког потенцијала и технологија производње крмног биља у функцији одрживог развоја сточарства (RS-MESTD-Technological Development (TD or TR)-31057)
DOI: 10.2298/GENSR1601187V
ISSN: 0534-0012
WoS: 000376744800016
Scopus: 2-s2.0-84971219286
Институција/група
Institut za voćarstvoTY - JOUR AU - Vasić, Tanja AU - Krnjaja, Vesna AU - Jevremović, Darko AU - Stanković, Slavica AU - Terzić, Dragan AU - Milenković, Jasmina AU - Marković, Jordan PY - 2016 UR - https://refri.institut-cacak.org/handle/123456789/359 AB - A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover EP - 198 IS - 1 SP - 187 VL - 48 DO - 10.2298/GENSR1601187V UR - conv_1544 ER -
@article{ author = "Vasić, Tanja and Krnjaja, Vesna and Jevremović, Darko and Stanković, Slavica and Terzić, Dragan and Milenković, Jasmina and Marković, Jordan", year = "2016", abstract = "A total of 17 isolates of Colletofrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non utilising (nit) mutants were isolated from each of investigated C. desfructivum isolates by selecting chlorate -resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self -incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletofrichum species. An identical profile was produced for all C. desfructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. desfructivum isolates.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover", pages = "198-187", number = "1", volume = "48", doi = "10.2298/GENSR1601187V", url = "conv_1544" }
Vasić, T., Krnjaja, V., Jevremović, D., Stanković, S., Terzić, D., Milenković, J.,& Marković, J.. (2016). Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 48(1), 187-198. https://doi.org/10.2298/GENSR1601187V conv_1544
Vasić T, Krnjaja V, Jevremović D, Stanković S, Terzić D, Milenković J, Marković J. Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover. in Genetika-Belgrade. 2016;48(1):187-198. doi:10.2298/GENSR1601187V conv_1544 .
Vasić, Tanja, Krnjaja, Vesna, Jevremović, Darko, Stanković, Slavica, Terzić, Dragan, Milenković, Jasmina, Marković, Jordan, "Vegetative compatibility and RFLP analysis of colletotrichum destructivum isolates from alfalfa and red clover" in Genetika-Belgrade, 48, no. 1 (2016):187-198, https://doi.org/10.2298/GENSR1601187V ., conv_1544 .